Biodegradation of Premium Motor Spirit using surfactant-expressing bacteria from mechanic workshops in Malumfashi, Katsina State, Nigeria

Abstract

This study was aimed at investigating the potential use of biosurfactant-expressing bacterial isolates in the biodegradation of premium motor spirit (PMS) at various concentrations (100-100,000ppm). The biosurfactant-expressing bacteria were isolated from mechanic workshop in Malumfashi, Katsina, Nigeria using standard techniques. The isolates identified belonged to the genera Acinetobacter, Bacillus, Micrococcus, Pseudomonas and Stenotrophomonas. These isolates were screened for biosurfactant expression using drop collapse, haemolysis, oil–water behavior assays and emulsification index test. Positives isolates were investigated for PMS degradation by growing the isolates on mineral salt media supplemented with (0.1ml) premium motor spirit (PMS) as sole source of carbon. Although, higher total hydrocarbon degrading bacterial counts were obtained from soils where isolates positive for biosurfactant expression are predominant, there was no statistically significant difference between isolate source using Kruskal-Wallis H test (p = 0.67). The isolates Bacillus velezensis and Stenotrophomonas maltophilia were positive for biosurfactant-production potential using drop-collapse, β-haemolysis, oil spreading, and emulsification index and drop collapse tests with higher tolerance to PMS at concentrations up to 100,000 ppm. Statistical analysis using multiple-comparison analysis of variance (ANOVA) confirmed that the isolates exhibited varying PMS degradation response (p = 0.0066); furthermore, the tolerance of the bacteria to the PMS is dose-dependent (p = 0.00012). Post-hoc analysis using Tukey’s test identified Bacillus velezensis as the most efficient biosurfactant-producing and hydrocarbon degrading isolate (p = 0.0264 and 0.0034); moreover, the threshold concentration for high PMS tolerance was found to be 1000ppm and above (p = 0.0174, 0.0008 and 0.0001).These isolates’ ability to grow on mineral salt media supplemented with PMS as a sole source of carbon presents a veritable avenue for exploitation in biotechnology, towards biosurfactants-mediated bioremediation of hydrocarbon pollutants in oil contaminated soils.

Keywords:  Biodegradation, Petroleum Hydrocarbons, Premium Motor Spirit

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UJMR News

The Editorial Board of UJMR is glad to have published the Twelfth edition (Volume 7 Number 1, June, 2022). The Journal covers general and Applied Microbiology, Biotechnology, Biological Sciences, Biochemistry, Bioinformatics, Medical, Public Health and Environmental sciences.

This year, UMYU Journal of Microbiology Research (UJMR) has reached its 5th year of publication which started in 2016. Over these years, many changes have been made to the typeface and general formats of the manuscripts, both the print version and online copies, to make the Journal meet the international standard and more visually appealing. From volume 3 (2) onward, all the online copies of UJMR papers on our website have been uploaded with their full colours. In addition, both authors and co-author are issued with e-certificates of publication for their respective published manuscripts.

We are glad that UJMR is gaining wider acceptance within Nigeria and in some foreign countries, and the number of submissions to the Journal is increasing. Despite the increase in manuscripts, UJMR maintained its quality as reflected by the rigorous peer-reviewing process. All articles submitted are accepted for publication based on a fair, objective, and anonymous assessment by our editors backed by the cogent decision by the editorial board. UJMR has thus increased the number of her associate editors by bringing in more people from academia who are experts in various fields within the scope of the Journal to enhance/maintain the speedy review of manuscripts received from various authors. The UJMR website has been upgraded so that UJMR readers and authors could get vital services such as online submission of manuscripts, downloading UJMR archives, author guidelines, etc.

UJMR’s vision is to be an internationally recognised premier journal of Microbiology, to contribute toward high-quality research and dissemination of UJMR to reach a larger audience worldwide. The journal is now indexed with reputable indexing organizations such as Crossref, Index Corpernicus International, International Institute of Organized Research (I2OR), Google Scholar, CiteSeerX, among others. In addition, the Journal has scored a publication impact factor of 2.525 for the year 2020 and Index Corpernicus value (ICV) of 85.02. Moreover, all our published articles are now assigned DOI. We hope that anyone with the expertise would join us and collaborate with our effort to obtain a much higher Impact Factor. We are delighted that UJMR is the first University-based microbiology journal in Nigeria. Therefore, we urge our academic colleagues to join hands with us towards projecting scientific research.

We appreciate the tireless efforts of the editorial board members, our editorial advisers, associate editors, and authors that contributed towards making UJMR a high-quality research journal. The support extended to the Journal by the Vice Chancellor and Umaru Musa Yar’adua University management is highly appreciated.

 

Dr. Bashir Abdulkadir

Editor – in – Chief       

Abstracting and Indexing