Isolation, Screening and Characterization of Phosphate Solubilizing Bacteria from Rice Farm in Dutsen Kura Minna Niger State

Biofertilizer

Abstract

There is direct correlation between the unending rise in human population and food production. Phosphorus is considered to be the second vital macronutrient subsequent to Nitrogen in restricting crop growth and development. Soluble phosphate is highly reactive with elements such as iron (Fe), Aluminium (Al), and Calcium (Ca). which has been the factor hindering crop production. The study was conducted at department of microbiology laboratory federal university of technology Minna from June, 2024 to November, 2024.In this study effort was made to Isolate the Phosphate solubilizing bacteria. Soil sample was collected from depth of 6-15cm from rice farm and aseptically stored in polythene bags maintained at 4ºC in the laboratory for further study. Isolation were carried out using Pikovskaya’s medium followed by screening of the two-bacterial isolates selected with high Phosphorus Solubilization index and high phosphate concentration (mg/l). The best of pH and temperature for phosphorus solubilization was optimized. Both Isolates had highest phosphate concentration at neutral pH and 35 ºC. The Isolates were identified as Acinetobacter sp and Bacillus sp.