Larvicidal Activity of Spores and Metabolites Extract of Aspergillus Niger Against Culex Mosquito

Abstract

Mosquitoes are vectors for a variety of diseases, including, malaria, dengue, filariasis, Japanese encephalitis, chikungunya and yellow fever to humans and other domesticated animals. Culex quinquefasciatus mosquitoes are vectors of diseases like lymphatic filariasis, Japanese encephalitis, Ross River viral and West Nile Virus. Approximately 120 million individuals are affected globally. Repeated use of chemical insecticides is led to environmental pollution and deleterious effects on non-target organisms. Therefore, this research is aimed to evaluate the larvicidal effect of metabolites and spores extracts of Aspergillus niger against Culex mosquito. The lethal concentrations (LC50 and LC90) were calculated by Probit analysis. The highest mortality rate (100%) was recorded in highest concentrations of metabolites (40 mg/mL) extracts of Aspergillus niger. A remarkable mortality rate (100%) was recorded at spores concentration of 4.5× 108 CFU/ml. The crude metabolites chemical constituents were characterized using GC-MS analysis and FTIR analysis. The major components identified from the GC-MS analyses include, Cetene, Dodecanoic acid Tetradecanoic acid, n-Hexadecanoic acid, cis-Vaccenic acid, Oleic Acid, and 3-Eicosene, (E)-. The findings of this study clearly revealed that Aspergillus niger derived crude metabolites and spores extract have potential for controlling the mosquito vectors.