ANTIBACTERIAL POTENTIALS AND PYTOCHEMICAL ANALYSIS OF LEAVE EXTRACTS OF MENTHA AVENSIS

Abstract

 Nature has been a source of medicinal agents since time immemorial. The importance of herbs in the management of human ailments can not be over emphasized. In the present study the pytochemical analysis and antibacterial potential of Mentha avensis was asses against the activity of different strains of bacteria. The phytochemical screening involved the identification of various secondary metabolites, such as alkaloids, flavonoids phenols, tannins, terpenoids, and saponins. The anti-bacterial activities was assessed using the agar diffusion technique, while the broth dilution method was employed to determine the minimum inhibitory concentration (MIC) value respectively. The anti-bacterial activities of the plant extracts were evaluated against Salmonella paratyphi, Pseudomonas aeruginosa, and Staphylococcus aureus. The minimum inhibitory (MIC) value was taken as micro gram per miligram (μg/mL) the serial dilution was conducted starting concentration of the plants started with 40mg, 20mg, 10mg 5mg to 1.25mg respectively,Standard antibiotics (tetracycline)was employed as positive controls. The largest yield was obtained from ethanol fractional extract as compared with that of chloroform extracts. The zone of inhibition of Mentha arvensis leaves extract when used at a concentration of 40 mg/ml, the crude extract had similar antibacterial effects as the positive control tetracycline (0.1 mg/ml). From the extracts 10mg/ml crude ethanol extract of Mentha arvensis was observed to suppress the growth of Salmonella paratyphi, Pseudomonas aeruginosa, and Staphylococcus aureus, according to the MIC values. At various concentration all the extracts showed the highest inhibition zone diameter against Salmonella paratyphi. The phytochemical components discovered in the leaves of Mentha avensis included alkaloids, flavonoids, saponins, and tannins. Among these, the tannin content was remarkably higher. The current results could provide a rational support for the traditional use of Mentha avensis  in primary healthcare due to it high antibacterial potentials.