Survey of bla genes in UPEC among patients attending Federal Teaching Hospital and General Hospital Katsina

Abstract

The presence of bla genes (bla_TEM, bla_SHV and bla_CTX-M) among extended beta-lactamase producing UPEC were investigated from Federal Teaching Hospital Katsina (FTHK) and General Hospital Katsina (GHK) patients. In vitro antibiotic susceptibility test was conducted using disc diffusion method. Phenotypic identification of extended ß-lactamase-producing (ESBL) UPEC was confirmed using double disc synergy test (DDS). The phenotypically positive ESBL isolates were molecularly characterized for the presence of bla_TEM, bla_SHV and bla_CTX-M genes by polymerase chain reaction (PCR). Three (3) UPEC isolates from a total of (n=25) in FTHK and two (2) UPEC isolates from a total of (n=20) in GHK patients harbored either of the bla genes. The overall incidence of bla-genes in the study population was found to be 11.11%. A total of five (5) isolates from both hospitals harbored ESBL-genes. The frequency of UPEC antibiotic resistance varied in both hospitals. Continuous surveillance and routine clinical detection of ESBL-producing organisms are necessary to prevent public health problems in clinical settings.

Keywords: Uropathogenic Escherichia coli, Phenotypic, Polymerase chain reaction, Antibiotics