Antimicrobial Effects of Ocimum gratissimum Extracts on the Spoilage Organisms Isolated from Yoghurt Samples
DOI:
https://doi.org/10.47430/ujmr.25103.030Keywords:
Ocimum gratissimum, antimicrobial effects, yoghurt, minimum inhibitory concentration, minimum cidal concentrationAbstract
Study’s Excerpt:
- OG extracts inhibited major spoilage organisms in stored yoghurt samples.
- Ethanolic OG extract showed higher activity than aqueous extract at same concentrations.
- MIC of ethanolic extract was as low as 31.25 mg/mL for subtilis, Bacillus spp., and A. niger.
- MCC values confirmed strong cidal action, especially in ethanolic extract.
- OG extracts show promise as natural yoghurt preservatives.
Full Abstract:
Ocimum gratissimum (OG) is recognised for its nutritional, antioxidant, and antimicrobial properties, making it a strong candidate for natural food preservation. This study evaluated the effects of ethanolic and aqueous OG leaf extracts against spoilage organisms isolated from laboratory-prepared yoghurt samples stored at room temperature for ten days. The agar well diffusion technique was employed for antimicrobial screening, while the broth microdilution method was used to determine both minimum inhibitory concentration (MIC) and minimum cidal concentration (MCC). The isolated organisms included Bacillus subtilis, Bacillus spp., Saccharomyces cerevisiae, Saccharomyces bulderi, Pichia kudriavzevii, Aspergillus niger, and Aspergillus flavus, identified by standard microbiological methods. The aqueous extract at a concentration as low as 62.50 mg/mL inhibited Bacillus subtilis, Saccharomyces bulderi, Pichia kudriavzevii, and Saccharomyces cerevisiae, with inhibition zones ranging from 5.00±0.27 to 6.20±0.87 mm. The ethanolic extract exhibited antimicrobial effects against Bacillus subtilis, Bacillus spp., Saccharomyces bulderi, Pichia kudriavzevii, and Saccharomyces cerevisiae at the same concentration (62.50 mg/mL), but with larger inhibition zones ranging from 5.30±0.45 to 11.35±2.10 mm. The aqueous extract showed substantial inhibition of Bacillus subtilis, Saccharomyces bulderi, and Pichia kudriavzevii, with an MIC of 62.50 mg/mL. In contrast, the ethanolic extract demonstrated an MIC of 31. 25 mg/mL for Bacillus subtilis, Bacillus spp., and Aspergillus niger. The MCC of the aqueous extract was 250 mg/mL for Bacillus subtilis, Saccharomyces bulderi, Pichia kudriavzevii, Aspergillus flavus, and Saccharomyces cerevisiae, while the MCC of the ethanolic extract was 125 mg/mL for Bacillus subtilis and Bacillus spp. Overall, the OG extracts exhibited substantial antimicrobial effects, effectively inhibiting the growth of spoilage organisms isolated from stored yoghurt samples. Harnessing the potential of OG can lead to an extended shelf life for yoghurt, positioning OG as a promising natural preservative.
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References
Abu-Zaid, A. A., Al-Barty, A., Morsy, K., & Hamdi, H. (2022). In vitro study of antimicrobial activity of some plant seeds against bacterial strains causing food poisoning diseases. Brazilian Journal of Biology, 82, 1–7. https://doi.org/10.1590/1519-6984.256409
Abu, M. L. (2024). A Review of the Efficiency of Ocimum gratissimum as a Vegetable Condiment. African Journal of Agriculture and Food Science, 7(2), 219–230. https://doi.org/10.52589/ajafs-cksmj20q
Akinduti, P. A., Motayo, B., Idowu, O. M., Isibor, P. O., Olasehinde, G. I., Obafemi, Y. D., Ugboko, H. U., Oyewale, J. O., Oluwadun, A., & Adeyemi, G. A. (2019). Suitability of spectrophotometric assay for determination of honey microbial inhibition. Journal of Physics: Conference Series, 1299(1). https://doi.org/10.1088/1742-6596/1299/1/012131
Akwetey, W. Y., Adzitey, F., & Teye, G. A. (2021). Cured Characteristics, Physicochemical Properties and Sensory Profile of Frankfurters Produced with Ocimum gratissimum Extract Leaf Extracts. Food Science & Nutrition Research, 4(1), 3–7. https://doi.org/10.33425/2641-4295.1040
Alexander, P. (2016). Phytochemical Screening and Mineral Composition of the Leaves of Ocimum gratissimum (Scent Leaf). International Journal of Applied Sciences and Biotechnology, 4(2), 161–165. https://doi.org/10.3126/ijasbt.v4i2.15101
Amenu, K., Wieland, B., Szonyi, B., & Grace, D. (2019). Milk handling practices and consumption behaviour among Borana pastoralists in southern Ethiopia. Journal of Health, Population, and Nutrition, 38(1), 6. https://doi.org/10.1186/s41043-019-0163-7
Anagboso, O., Ohaebuka, J. C, Mbah, I. E, Osuala, J.O., Daokoru-Olukole, G. C, Onu, N. E, Agedah, C. E, Nwankwo, A. G., Okafor, O. F, Silas-Olu, D.,& Okonko,O. (2024). Microbial contamination and antibiotic resistance in selected yoghurt brands inElele River State Nigeria12(2), 1–7.
Atwaa, E. S. H., Shahein, M. R., El-Sattar, E. S. A., Hijazy, H. H. A., Albrakati, A., & Elmahallawy, E. K. (2022). Bioactivity, Physicochemical and Sensory Properties of Probiotic Yoghurt Made from Whole Milk Powder Reconstituted in Aqueous Fennel Extract. Fermentation, 8(2). https://doi.org/10.3390/fermentation8020052
Azuamah, Y., Uguru, K., Eluchie, E., & Johnson, C. (2024). Antibacterial activity of Ocimum gratissimum (scent leaf) extracts on selected bacteria isolates. Journal of Medicine and Biomedical Research, 23(1), 36–44. https://doi.org/10.4314/jmbr.v23i1.7
Chimezie, G. D., Gloria, L., & Ebere, I. (2015). Microbiological load of yoghurt sold in Omoku schools, Rivers State, Nigeria. African Journal of Microbiology Research, 9(34), 1960–1963. https://doi.org/10.5897/ajmr2015.7556
Ebabhi, A. M., Adeogun, O. O., Adekunle, A. A., & Onafeko, A. O. (2019). Bio-preservation Potential of Leaf Extracts of Ocimum gratissimum L. on Fresh-Cut Fruits of Citrullus lanatus (Thunb). Journal of Applied Sciences and Environmental Management, 23(7), 1383. https://doi.org/10.4314/jasem.v23i7.30
Edo, G. I., Samuel, P. O., Ossai, S., Nwachukwu, S. C., Okolie, M. C., Oghenegueke, O., Akpoghelie, P. O., Ugbune, U., Ezekiel, G. O., Onoharigho, F. O., & Agbo, J. J. (2023). Phytochemistry and Pharmacological Compounds Present in Scent Leaf. A Review. Food Chemistry Advances, 100300. https://doi.org/10.1016/j.focha.2023.100300
Faraz, A. (2022). Milk homogenisation and standardisation. Rural Development by Livestick Extension Education in Southern Punjab, 5(8), 1–10.
Gajic, I., Kabic, J., Kekic, D., Jovicevic, M., Milenkovic, M., Mitic Culafic, D., Trudic, A., Ranin, L., & Opavski, N. (2022). Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods. Antibiotics, 11(4), 1–26. https://doi.org/10.3390/antibiotics11040427
Ginovyan, M., Petrosyan, M., & Trchounian, A. (2017). Antimicrobial activity of some plant materials used in Armenian traditional medicine. BMC Complementary and Alternative Medicine, 17(1), 1–9. https://doi.org/10.1186/s12906-017-1573-y
Hidayat, R., & Patricia Wulandari. (2021). Methods of Extraction: Maceration, Percolation and Decoction. Eureka Herba Indonesia, 2(1), 73–79. https://doi.org/10.37275/ehi.v2i1.15.
Ibrahim, A. N., Igwe, C. E., Asogwa, I. S., Agbaka, J. I., & Ajibo, Q. C. (2020b). Influence of Ocimum gratissimum (Scent Leaf) on the Organoleptic Acceptability and Shelf Stability of Yoghurt. Asian Food Science Journal, 16(1), 28–44. https://doi.org/10.9734/afsj/2020/v16i130163
Kovalchuk, Y. (2021). Bio-based preservatives : A natural alternative to synthetic additives. 01(December 2020), 56–70.
Le Ba, T., Dam, M. S., Nguyen, L. L. P., Baranyai, L., & Kaszab, T. (2025). A Review of Processing Techniques and Rheological Properties of Yogurts. Journal of Texture Studies, 56(1). https://doi.org/10.1111/jtxs.70006
Mafe, A. N., Edo, G. I., Makia, R. S., Joshua, O. A., Akpoghelie, P. O., Gaaz, T. S., Jikah, A. N., Yousif, E., Isoje, E. F., Igbuku, U. A., Ahmed, D. S., Essaghah, A. E. A., & Umar, H. (2024). A review on food spoilage mechanisms, food borne diseases and commercial aspects of food preservation and processing. Food Chemistry Advances, 5(November), 100852. https://doi.org/10.1016/j.focha.2024.100852
Matasyoh, L. G., Matasyoh, J. C., Wachira, F. N., Kinyua, M. G., Thairu Muigai, A. W., & Mukiama, T. K. (2008). Antimicrobial activity of essential oils of Ocimum gratissimum L. from different populations of Kenya. African Journal of Traditional, Complementary and Alternative Medicines, 5(2), 187–193. https://doi.org/10.4314/ajtcam.v5i2.31272
Mehra, R., Kumar, H., Rafiq, S., Kumar, N., Buttar, H. S., Leicht, K., Okpala, C. O. R., & Korzeniowska, M. (2022). Enhancing yogurt products’ ingredients: preservation strategies, processing conditions, analytical detection methods, and therapeutic delivery—an overview. PeerJ, 10(October). https://doi.org/10.7717/peerj.14177
Melo, R. S., Azevedo, Á. M. A., Pereira, A. M. G., Rocha, R. R., Cavalcante, R. M. B., Matos, M. N. C., Lopes, P. H. R., Gomes, G. A., Rodrigues, T. H. S., Dos Santos, H. S., Ponte, I. L., Costa, R. A., Brito, G. S., Catunda, F. E. A., & Carneiro, V. A. (2019). Chemical composition and antimicrobial effectiveness of Ocimum gratissimum l. essential oil against multidrug-resistant isolates of Staphylococcus aureus and Escherichia coli. Molecules, 24(21). https://doi.org/10.3390/molecules24213864
Mureşan, C. C., Marc, R. A., Jimborean, M., Rusu, I., Mureşan, A., Nistor, A., Cozma, A., & Suharoschi, R. (2020). Food safety system (Haccp) as quality checkpoints in a spin-off small-scale yoghurt processing plant. Sustainability (Switzerland), 12(22), 1–20. https://doi.org/10.3390/su12229472
Nijris, O. (2021). Biochemical tests. Medical Microbiology Biochemical Test 1. February. https://doi.org/10.13140/RG.2.2.30399.84640
Nor-Khaizura, M. A. R., Flint, S. H., Mccarthy, O. J., Palmer, J. S., Golding, M., & Jaworska, A. (2018). “Made-in-transit” Yoghurt processing: A review of basic concepts and technological implications. International Journal of Food Studies, 7(2), 117–135. https://doi.org/10.7455/IJFS/7.2.2018.A10
OyebodeO. A., Oyelade W. A., &JejeO. A., (2023). Phytochemical screening and antibacterial activities of Ocimum gratissimum against some clinical isolates. GSC Biological and Pharmaceutical Sciences, 24(2), 148–153. https://doi.org/10.30574/gscbps.2023.24.2.0249
Okeke L. A., Clement O. A., David C. N., V. K. O. (2023). Phytochemical analysis and antibacterial activities of various leaf extracts of Ocimum gratissimum and Newbouldia laevis. GSC Biological and Pharmaceutical Sciences, 22(3), 144–152. https://doi.org/10.30574/gscbps.2023.22.3.0104
Onasanya, G. O., Adeleye, A., & Saidu, S. (2019).Detection of bacterial contaminants in locally processed yoghurt : evidence from bacteriological analyses FudmaJournal of Agriculture and Agricultural Technology ISSN : 2504-9496. December.
Onyebuchi, C., & Kavaz, D. (2020). Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts. Scientific Reports, 10(1), 1–11. https://doi.org/10.1038/s41598-020-78847-5
Oyebode O.A., Oyelade W.A., Jeje. O. A. (2023). Phytochemical screening and antibacterial activities of Ocimum gratissimum against some clinical isolates. GSC Biological and Pharmaceutical Sciences, 24(2), 148–153. https://doi.org/10.30574/gscbps.2023.24.2.0249
Oyeniyi, A. O., Aworh, O. C., J.O., & Olaniyan, &. (2014). Effect of Flavourings on Quality and Consumer Acceptability of Soy-Yoghurt. IOSR Journal of Environmental Science, Toxicology and Food Technology, 8(1), 38–44. https://doi.org/10.9790/2402-08133844
Pavithra, K. H. G. (2019). Qualitative and Quantitative Phytochemical Analysis on Ocimum Species of Karnataka. Indian Journal of Pure & Applied Biosciences, 7(6), 192–202. https://doi.org/10.18782/2582-2845.7876
Rodríguez-Melcón, C., Alonso-Calleja, C., García-Fernández, C., Carballo, J., & Capita, R. (2022). Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for Twelve Antimicrobials (Biocides and Antibiotics) in Eight Strains of Listeria monocytogenes. Biology, 11(1). https://doi.org/10.3390/biology11010046
Saleem, G. N., Gu, R., Qu, H., Bahar Khaskheli, G., Rashid Rajput, I., Qasim, M., & Chen, X. (2024). Therapeutic potential of popular fermented dairy products and its benefits on human health. Frontiers in Nutrition, 11(February), 1–17. https://doi.org/10.3389/fnut.2024.1328620
Shu’aibu, I., Abdullahi, H., Hanna Kadum, S., Hamza, A. J., Mustapha, Y. K., Muhammad, T. A., Garba, L., Muhammad, K., Nuru, A. G., Mani, M. A., & Abubakar, G. G. (2021). Raw Milk as a Potential Source of Food Poisoning Outbreaks. Journal of Environmental Bioremediation and Toxicology, 4(2), 23–26. https://doi.org/10.54987/jebat.v4i2.629
Shwetnisha and Nongmaithem Mangalleima, R. S. M. N. (2021). The Product and the Manufacturing of Yoghurt. International Journal for Modern Trends in Science and Technology, 7(10), 48–51. https://doi.org/10.46501/ijmtst0710007
Silva, J. C., Silva Pereira, R. L., Sampaio de Freitas, T., Rocha, J. E., Macedo, N. S., de Fatima Alves Nonato, C., Linhares, M. L., Arruda Tavares, D. S., Bezerra da Cunha, F. A., Melo Coutinho, H. D., Gonçalo de Lima, S., Pereira-Junior, F. N., Araújo Maia, F. P., Pita Neto, I. C., Galvão Rodrigues, F. F., & Garcia Santos, G. J. (2022). Evaluation of antibacterial and toxicological activities of essential oil of Ocimum gratissimum L. and its major constituent eugenol. Food Bioscience, 50. https://doi.org/10.1016/j.fbio.2022.102128
Sinmiat Abiodun, O., Felix Akinsola, A., TT, A., & AO, O. (2020). Effects of Ocimum gratissimum and Citric Acid on the Nutritive and Sensory Qualities of Stored Pineapple Juice. Journal of Food Science and Nutrition Research, 03(02), 34–49. https://doi.org/10.26502/jfsnr.2642-11000037
Soola, J. O., Kukoyi, B. I., Olooto, W. E., Adenusi, H. A., & Monisola, A. (2021). Phytochemical ( qualitative, quantitative ), proximate analysis, toxicological implication and characterisation of aqueous Ocimum gratissimum leaf extract. Nigerian Journal of Pharmaceuticals and Applied Science Research, 10(3), 1–9.
Sukhdev S. H., Suman P. S. K., Gennaro L., D. D. R. (2008). Extraction techniques of Medicinal plants and aromatic plants. International Centre for Science and High Technology, 1–10. http://scioteca.caf.com/bitstream/handle/123456789/1091/RED2017-Eng8ene.pdf?sequence=12&isAllowed=y%0Ahttp://dx.doi.org/10.1016/j.regsciurbeco.2008.06.005%0Ahttps://www.researchgate.net/publication/305320484_SISTEM_PEMBETUNGAN_TERPUSAT_STRATEGI_MELESTARI
Swebocki, T., Barras, A., Maria Kocot, A., Plotka, M., & Boukherroub, R. (2023). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays using broth microdilution method. Protocols, Mic, 1–11. https://doi.org/10.17504/protocols.io.5qpvo3x6dv4o/v1
Talabi, J. Y., & Makanjuola, S. A. (2017). Proximate, phytochemical, and in vitro antimicrobial properties of dried leaves from Ocimum gratissimum. Preventive Nutrition and Food Science, 22(3), 191–194. https://doi.org/10.3746/pnf.2017.22.3.191
Tanko, Y1. Magaji, G. M. Yerima, M. Magaji, R. A.Mohammed, A. (2008). Anti-nociceptive and anti-inflammatory activities of aqueous leaves extract of Ocimum gratissimum ( labiate ) in rodents.Afr. J. Traditional, Complementary and Alternative Medic, 5(2), 141–146. https://doi.org/10.4314/ajtcam.v5i2.31265
Tordzagla, N., Kansake, B., & Bakang, R. (2024). Evaluation of phytochemicals , antimicrobial activity and antioxidant potential of methanolic extract of Vitellaria paradoxa nut ( shea nut ).
Ugbogu, O. C., Emmanuel, O., Agi, G. O., Ibe, C., Ekweogu, C. N., Ude, V. C., Uche, M. E., Nnanna, R. O., & Ugbogu, E. A. (2021). A review on the traditional uses, phytochemistry, and pharmacological activities of clove basil (Ocimum gratissimum L.). Heliyon, 7(11), e08404. https://doi.org/10.1016/j.heliyon.2021.e08404
Unegbu, V., Ndidi, O. C., & Nkwoemeka, N. E. (2020). Phytochemical and antibacterial activities of Ocimum gratissimum. August 2023.
Wang, X., Wang, L., Wei, X., Xu, C., Cavender, G., Lin, W., & Sun, S. (2025). Invited review: Advances in yoghurt development—Microbiological safety, quality, functionality, sensory evaluation, and consumer perceptions across different dairy and plant-based alternative sources. Journal of Dairy Science, 108(1), 33–58. https://doi.org/10.3168/jds.2024-25322
Xie, Y., Zhang, C., Mei, J., & Xie, J. (2023). Antimicrobial Effect of Ocimum gratissimum L. Essential Oil on Shewanella putrefaciens: Insights Based on the Cell Membrane and External Structure. International Journal of Molecular Sciences, 24(13). https://doi.org/10.3390/ijms241311066
Zhang, Q. W., Lin, L. G., & Ye, W. C. (2018). Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Medicine (United Kingdom), 13(1), 1–26. https://doi.org/10.1186/s13020-018-0177-x
Zouine, N., Ghachtouli, N. El, Abed, S. El, & Koraichi, S. I. (2024). A comprehensive review on medicinal plant extracts as antibacterial agents: Factors, mechanism insights and future prospects. Scientific African, 26(August), e02395. https://doi.org/10.1016/j.sciaf.2024.e02395
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