Seroprevalence and Socioeconomic Characteristics of Human Sapovirus Infection among Children Attending Specialist Hospital, Sokoto, Sokoto State, Nigeria
DOI:
https://doi.org/10.47430/ujmr.25103.036Keywords:
ELISA, IgG/IgM, Prevalence, SapovirusAbstract
Study’s Excerpt:
- Sapovirus prevalence among children aged 0–5 in Sokoto was 7.7%.
- Highest infection rate (17.6%) occurred in children aged 49–60 months.
- Rural children had higher infection (8.2%) than urban counterparts.
- Parents with secondary education level showed highest infection (3.3%).
- Farmers’ children recorded the highest sapovirus prevalence (3.3%).
Full Abstract:
The study investigates the seroprevalence and socioeconomic characteristics of human sapovirus infection among children aged 0-5 years attending a specialist hospital in Sokoto, Sokoto State, Nigeria. A total of 100 blood samples were collected for human sapovirus detection and Sapovirus IgG/IgM. A questionnaire was used to obtain the socioeconomic characteristics associated with human sapovirus infection. Sapovirus IgG/IgM was detected using ELISA kit. The results showed a prevalence of 7.70% sapovirus infection among children. There was an association of sapovirus among males (4.40%) and females (3.30%). Children within 49-60 months had the highest sapovirus infection of 17.60% while Parents/Guidance with secondary level of education had the highest sapovirus infection of 3.30%. Parents/Guidance residing in the rural areas had a higher sapovirus infection rate of 8.20%. Children living in families of 4-6 had the highest sapovirus infection rate of 4.0%. There was an association between the occupational status of the children's parents/guidance and the prevalence of sapovirus infection; farmers had the highest prevalence of 3.30%. The serological assays (ELISA) provide information about the prevalence of HSaV infection among children in the study area. Routine testing for all enteric viruses, especially Human Sapovirus, is needed.
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Ahmed, M. U., Kobayashi, N., Wakuda, M., Sanekata, T., Taniguchi, K., Kader, A., & Mise, K. (2004). Genetic analysis of group B human rotaviruses detected in Bangladesh in 2000 and 2001. Journal of Medical Virology, 72(1), 149–155. https://doi.org/10.1002/jmv.10546
Alhassan, J., Dadari, S. A., Shebayan, J. A., & Babaji, B. A. (2012). Phytosociological attributes of weeds in lowland paddy at Talata Mafa. International Journal of Agronomy and Agricultural Research, 6(4), 8–13.
Al-Shuwaikh, A. M. (2016). Frequency of rotavirus, adenovirus and astrovirus among patients with acute diarrhea by chromatographic immunoassay and enzyme-linked immunosorbent assay. Journal of Biotechnology Research Center, 10(2), 58–64. https://doi.org/10.24126/jobrc.2016.10.2.485
Amar, C. F., East, C. L., Gray, J., Iturriza-Gomara, M., Maclure, E. A., & McLauchlin, J. (2007). Detection by PCR of eight groups of enteric pathogens in 4,627 faecal samples: Re-examination of the English case-control Infectious Intestinal Disease Study (1993–1996). European Journal of Clinical Microbiology & Infectious Diseases, 26, 311–323. https://doi.org/10.1007/s10096-007-0290-8
Becker-Dreps, S., Bucardo, F., & Vinjé, J. (2019). Sapovirus: An important cause of acute gastroenteritis in children. The Lancet Child & Adolescent Health, 3(11), 758–759. https://doi.org/10.1016/S2352-4642(19)30270-6
Becker-Dreps, S., Bucardo, F., Vilchez, S., Zambrana, L. E., Liu, L., Weber, D. J., Peña, R., Barclay, L., Vinjé, J., Hudgens, M. G., Nordgren, J., Svensson, L., Morgan, D. R., Espinoza, F., & Paniagua, M. (2014). Etiology of childhood diarrhea after rotavirus vaccine introduction: A prospective, population-based study in Nicaragua. The Pediatric Infectious Disease Journal, 33(11), 1156–1163. https://doi.org/10.1097/INF.0000000000000427
Biscaro, V., Piccinelli, G., Gargiulo, F., Laniro, G., Caruso, A., Caccuri, F., & De Francesco, M. A. (2018). Detection and molecular characterisation of enteric viruses in children with acute gastroenteritis in Northern Italy. Infection, Genetics and Evolution, 60, 35–41. https://doi.org/10.1016/j.meegid.2018.02.011
Dey, S. K., Phan, T. G., Nguyen, T. A., Nishio, O., Salim, A. F. M., Yagyu, F., Okitsu, S., & Ushijima, H. (2007). Prevalence of sapovirus infection among infants and children with acute gastroenteritis in Dhaka City, Bangladesh during 2004–2005. Journal of Medical Virology, 79(5), 633–638. https://doi.org/10.1002/jmv.20859
Fazeli, Z., Baghaie, N., Khavarinejad, R. A., Khoramdel, M., Sigaroodi, A., & Nadji, S. A. (2016). Hospital-based study of prevalence and genotyping of noroviruses and sapoviruses isolated from children with acute gastroenteritis referred to Masih Daneshvari Hospital. Gastroenterology and Hepatology from Bed to Bench, 9(2), 91–97.
Gelaw, A., Pietsch, C., Mann, P., & Liebert, U. G. (2019). Molecular detection and characterisation of sapoviruses and noroviruses in outpatient children with diarrhea in Northwest Ethiopia. Epidemiology & Infection, 147, 218. https://doi.org/10.1017/S0950268819001031
Hansman, G. S., Oka, T., Katayama, K., & Takeda, N. (2007). Human sapoviruses: Genetic diversity, recombination, and classification. Reviews in Medical Virology, 17(2), 133–141. https://doi.org/10.1002/rmv.533
Hussein, R. A. (2016). Evaluation of multiplex real-time PCR and ELISA in detection of intestinal protozoan parasites and co-infection with rotavirus from children with diarrheal disease [PhD thesis]. College of Medicine, Al-Nahrain University, Iraq.
Japhet, M. O., Famurewa, O., Adesina, O. A., Opaleye, O. O., Wang, B., Höhne, M., Bock, C. T., Mas Marques, A., & Niendorf, S. (2019). Viral gastroenteritis among children of 0–5 years in Nigeria: Characterisation of the first Nigerian aichivirus, recombinant noroviruses and detection of a zoonotic astrovirus. Journal of Clinical Virology, 111, 4–11. https://doi.org/10.1016/j.jcv.2018.12.004
Kgomotso, M., Kwana, L., Sikhulile, M., David, M., Goldfarb, J., Pernica, M., Lemme, P., & Kebaabetswe, N. (2020). Molecular characterisation of sapovirus from children with gastroenteritis in Botswana. Journal of Clinical Virology Plus, 2(5), 100077–100085. https://doi.org/10.1016/j.jcvp.2022.100077
Khira, S., Mouna, H., Jean-Baptiste, B., Katia, A. B., Maha, M. A., Hakima, G., Zaidoun, A., Nabil, S., Mohamed, N.-G., Pierre, P., & Mahjoub, A. (2010). Aichi virus IgG seroprevalence in Tunisia parallels genomic detection and clinical presentation in children with gastroenteritis. Clinical and Vaccine Immunology, 17(7), 1111–1116. https://doi.org/10.1128/CVI.00059-10
Liu, X., Yamamoto, D., Saito, M., Imagawa, T., Ablola, A., Tandoc, A. O., Segubre-Mercado, E., & Lupisan, S. P. (2016). Molecular detection and characterisation of sapovirus in hospitalised children with acute gastroenteritis in the Philippines. Journal of Clinical Virology, 68, 83–88. https://doi.org/10.1016/j.jcv.2015.05.001
Magwalivha, M., Kabue, J. P., & Traore, A. N. (2018). Prevalence of human sapovirus in low and middle income countries. Advances in Virology, 2018, 5986549. https://doi.org/10.1155/2018/5986549
Mariam, S. A., Arwa, M. A., & Ismail, I. L. (2020). Detection and comparison of sapovirus, rotavirus and norovirus among infants and young children with acute gastroenteritis. Annals of Tropical Medicine and Public Health, 23(S14), SP231439. https://doi.org/10.36295/ASRO.2020.231439
Maysaa, E. S., Zaki, R., Shrief, R., & Hassan, H. (2022). Molecular study of sapovirus in acute gastroenteritis in children: A cross-sectional study. F1000Research, 10, 123. https://doi.org/10.12688/f1000research.29991.2
Nakata, S., Chiba, S., Terashima, H., Yokoyama, T., & Nakao, T. (2007). Humoral immunity in infants with gastroenteritis caused by human calicivirus. Journal of Infectious Diseases, 152(2), 274–279. https://doi.org/10.1093/infdis/152.2.274
National Population Commission (NPC). (2006). Nigeria national census: Population distribution by sex, state, LGAs and senatorial district (Vol. 3, pp. 5, 76–78).
Oka, T., Wang, Q., Katayama, K., & Saif, L. J. (2015). Comprehensive review of human sapoviruses. Clinical Microbiology Reviews, 28(1), 32–53. https://doi.org/10.1128/CMR.00011-14
Pang, X. L., Lee, B. E., Tyrrell, G. J., & Preiksaitis, J. K. (2001). Epidemiology and genotype analysis of sapovirus associated with gastroenteritis outbreaks in Alberta. Journal of Infectious Diseases, 199(4), 547–551. https://doi.org/10.1086/596210
Petronella, A. A. (2012). Prevalence and epidemiology of enteric viruses in children attending Lwak Mission Hospital in Asembo, Western Kenya [Master's thesis, Jomo Kenyatta University of Agriculture and Technology].
Romani, S., Azimzadeh, P., Mohebbi, S. R., Bozorgi, S. M., Zali, N., & Jadali, F. (2012). Prevalence of sapovirus infection among infant and adult patients with acute gastroenteritis in Tehran, Iran. Gastroenterology and Hepatology from Bed to Bench, 5(1), 43–54.
Saito, M., Goel-Apaza, S., Espetia, S., Velasquez, D., Cabrera, L., Loli, S., Crabtree, J. E., Black, R. E., Kosek, M., Checkley, W., Zimic, M., Bern, C., Cama, V., Gilman, R. H., & Norovirus Working Group in Peru. (2014). Multiple norovirus infections in a birth cohort in a Peruvian periurban community. Clinical Infectious Diseases, 58(4), 483–491. https://doi.org/10.1093/cid/cit763
Sanchez, G. J., Mayta, H., Pajuelo, M. J., Neira, K., Xiaofang, L., Cabrera, L., Ballard, S. B., Crabtree, J. E., Kelleher, D., & Cama, V. (2018). Epidemiology of sapovirus infections in a birth cohort in Peru. Clinical Infectious Diseases, 66(12), 1858–1863. https://doi.org/10.1093/cid/cix1103
Svraka, S., Vennema, H., van der Veer, B., Hedlund, K. O., Thorhagen, M., & Siebenga, J. (2010). Epidemiology and genotype analysis of emerging sapovirus-associated infections across Europe. Journal of Clinical Microbiology, 48(6), 2191–2198. https://doi.org/10.1128/JCM.02427-09
Yamashita, T., Ito, M., Tsuzuki, H., & Sakae, K. (2001). Identification of Aichi virus infection by measurement of immunoglobulin responses in an enzyme-linked immunosorbent assay. Journal of Clinical Microbiology, 39(11), 4178–4180. https://doi.org/10.1128/JCM.39.11.4178-4180.2001
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