Physicochemical and Characterization of Nitrogen Fixing Bacteria from Soil Samples Within the Vicinity of Telecommunication Mast (Site No: 000148) Located at Karfi Town Kura Local Government, Kano State
DOI:
https://doi.org/10.47430/ujmr.2161.010Keywords:
Non symbiotic Bacteria, telecommunication mast.Abstract
The telecommunication mast associated-radiation is one of the primary factors influencing the way in which microorganisms interact with ecosystem. This study aims to assess the physicochemical and non-symbiotic nitrogen fixing bacteria (NNFB) from soil samples within the vicinity of telecommunication mast located at Karfi town Kura local government Kano state. Soil samples A, B, and C were collected within the vicinity of the mast at an interval of 10 meters, 20 meters and 30 meters from the mast respectively and control sample (D) was collected from location *(outside the vicinity of the mast)*. Physicochemical parameters of the soil samples were analyzed, isolation and identification of non-symbiotic nitrogen fixing bacteria were carried out using standard procedures. Samples B showed higher values of pH (8.02), phosphorus (23.95mg/kg), organic carbon (1.45%), nitrogen (0.28%) and organic matter content (2.50%) while control sample (D) showed lower values with 6.24, 2.77mg/kg, 0.41%, 0.07% and 0.71% of pH, phosphorus, organic carbon, nitrogen and organic matter content respectively. However, the moisture content(0.21%) of control sample is higher than that of sample A and B with 0.12% and 0.11% respectively The mean count of NNFB of the soil samples were 3.20 ± 0.06, 1.80± 0.12, 1.40±0.23, 1.20±0.20 for sample B, C, A and D respectively. Total of 14 isolates of the species Azomonas agilis 1(7.14%), Azotomonas insolita 1(7.14%), Bacillus megaterium 2(14.28 %), Bacillus azotoformans 1(7.14%), Bacillus mycoides 3(21.42%), Enterobacter cloacae 3(21.42%), and Klebsiella pneumonia 3(21.42%) were obtained. This indicates that the electromagnetic radiation from the mast has no effect on soil physicochemical parameters as well as non symbiotic nitrogen fixing bacteria proliferation.
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References
Adebayo, E.A., Adeeyo, A.O., Ayandele, A.A. and Omomowo, I.O. (2014). Effect of Radiofrequency Radiation from Telecommunication Based Stations on Microbial Diversity and Antibiotic Resistance. Journal of Applied Science and Environmental Management, 18(4): 669-674.
https://doi.org/10.4314/jasem.v18i4.16
Ahmed, I., Istivan, T., Cosic, I and Pirogova, E (2013). Irradiation of E. coli by Extremely Low Frequency (ELF) Pulsed Electromagnetic Field: Evaluation of Bacterial Survival. Journal of Electromagnetic Waves, 29(1):26-37.
https://doi.org/10.1080/09205071.2014.974684
Balmori, A. (2016). Radiotelemetry and Wildlife: Highlighting a Gap in the Knowledge on Radiofrequency Radiation Effects. Science of the Total Environment, 543 (pt A), 662-669.
https://doi.org/10.1016/j.scitotenv.2015.11.073
Belbe, M.G. and Tofana, M.(2010).Effects of Ionizing Radiation on Microbiological Contaminants of Food. Bulletin UASVM Agriculture, 67(2) Pp.179-181
https://doi.org/10.15835/buasvmcn-agr:5052
Belyaev, I. (2005). Non-thermal Biological Effects of Microwaves. Microwave Review, 11: 13-29
Belyaev, I. (2011). Toxicity and SOS-response to ELF Magnetic Fields and Nalidixic Acid in E. coli cells. Mutation Research, 722: 56- 61.
https://doi.org/10.1016/j.mrgentox.2011.03.012
Benton, J.J. (1999). Soil Analysis Handbook of Reference Methods. Soil and Plant Analysis Council.Inc. Soil Science. 2nd ed. CRC Press.
Cheesbrough M. (2006). Medical Laboratory Practice inTropical countries. Part 2, 2nd ed. United Kingdom: Cambridge University Press; Page 157-200.
https://doi.org/10.1017/CBO9780511543470
Ding, Y., Wang, J., Liu, Y. and Chen, S. (2005). Isolation and Identification of Nitrogen Fixing Bacilli from Plant Rhizospheres in Beijing Region. Journal of Applied Microbiology, 99: 1271 - 1281
https://doi.org/10.1111/j.1365-2672.2005.02738.x
Emmyrafedziawati, A.K.R. and Stella, M. (2018). Identification of Free Living Nitrogen Fixing Bacteria Isolated From EFB Compost, Molecular Detection of NifH Gene and Measurement of Nitrogenase Activity. Journal of Tropical Agriculture and Food Science, 46(1): 39-46.
Eno, J.U., Ibia, T.O., Ogunwale, J.A., Ano, A.O. and Esu I.E. (2009). Manual of Soil, Plant and Water Analysis. Sibon Book Publishers Lagos .Pp 6-7.
Fomenky, N.N., Tening, A.S., Chuyong, B.G., Mbene, K., Asongwe, G.A. and Vivian,B.C. (2018). Selected Physicochemical Properties and Quality of Soils Around Some Rivers of Cameroon. Journal of Soil Science and Environmental Management, 9(5): 68-80.
Gee, W.G. and Or, D. (2002). Particle Size Analysis. In: Method of Soil Analysis. Book series 5. Part 4. Soil Science Society of America. Pp 255-293
https://doi.org/10.2136/sssabookser5.4.c12
Habtamu A (2011) Land Use/Land Covers Change and Impacts of Jatropha on Soil Fertility: The Case of Mieso and Bati Districts, Ethiopia (M.Sc. Thesis) Haramaya University, Ethiopia. Pp. 11-30.
Hoffman-Findeklee, C., Gadkari, D. and Meyer, O. (2000). Superoxide-dependent Nitrogen Fixation. In Nitrogen Fixation: From Molecules to Crop Productivity. pp. 23-30.
https://doi.org/10.1007/0-306-47615-0_5
Ibrahim, U.B., Ijah, U.J.J., Riskuwa, M.L. and Manga, S.B. (2014).Microbial and Heavy Metals Qualities of Agricultural Soils of Tsafe Local Government Area in Zamfara State-Nigeria. Journal of Zoological and Bioscience Research, 1(2): 18-30.
Iheme, C.I., Nwaougikpe, R.N., Nwaogu, L.A., Ujowundu, C.O., Ukairo, D.I. and Orji, C.E. (2016). Effect of Electromagnetic Radiation (EMR) on Some Soil Physicochemical Parameters, Catalase and Dehydrogenase Activities. IOSR Journal of Environmental Science Toxicology and Food Technology (IOSR- JESTFT), 10(1): 25-34.
Kekane, S.S., Chavan, R.P., Shinde, D.N., Patil,C.L. and Sagar, S.S. (2015). A Review on Physico-chemical Properties of Soil. International Journal of Chemical Studies, 3(4): 29-32. Ku S.T, and Sangita, I. (2015). A Review on Role of Physico-Chemical Properties in Soil Quality. Chemical Science Review Letters, 4(13):57-66.
Li, X., Wang, Y. and Lu, X. (2017). Long term agricultural activity affects anthropogenic soil on Chinese loess plateu. Journal of Arid Land, 9: 678-687.
https://doi.org/10.1007/s40333-017-0026-2
Merlo, C. and Susana, G. (2014). Environmental Factors Associated with Heterotrophic Nitrogen Fixing Bacteria in Water, Sediment and Riparian Soil of Suquia River. Limnologica, 48: 71-79.
https://doi.org/10.1016/j.limno.2014.06.004
Nelson, D.W. and Sommers, L.E. (1982). Total Carbon, Organic Carbon, and Organic Matter. In: Page, A. L. and Keeney, D. R. (eds), Methods of Soil Analysis Part 2. Madison, WI. American Society of Agronomy. 539-579.
https://doi.org/10.2134/agronmonogr9.2.2ed.c29
Onyeze, R., Onah, G. and Cecilia, I. (2013). Isolation and characterization of nitrogen fixing bacteria in the soil. International Journal of Life Sciences, 2(3): 438-445
Reed, S.C., Cleverlend, C.C and Townsend, A.R (2011). Functional Ecology of Free Living Nitrogen Fixation: A Contemporary Perspective. Annual Review of Ecology Evol ution and Systematics. 42.489-512.
https://doi.org/10.1146/annurev-ecolsys-102710-145034
Rifat, H., Safdar, A., Ummay, A., Rabia, K. and Iftikhar, A. (2010). Soil Beneficial Bacteria and Their Role in Plant Growth Promotion: A Review. Annals of Microbiology, 60(4): 579-598.
https://doi.org/10.1007/s13213-010-0117-1
Rousk, J., Bengston, P. and Stemgren, E. (2010). Archael Abundance Across a pH Gradient in an Arable Soil and its Relationship to Bacterial and Fungal Growth Rates. Applied and Environmental Microbiology, 78(16): 5906-5911.
https://doi.org/10.1128/AEM.01476-12
Saleh, H.S., Alharbi, A.S., Faden , A.A and Wainwright ,M. (2018). Evaluation of the Effect of High Frequency Electromagnetic Field on Growth and Antibiotic Sensitivity of Bacteria. Saudi Journal of Biological science, 25(I):105-110
https://doi.org/10.1016/j.sjbs.2017.07.006
Sandip, S.B., Vaneet, K., Navdeep, S., Vasudha, S., Jaswinder, S., Jatinder, K.K., Avinash, KN (2016). Physico-chemical Properties and Heavy Metal Contents of Soils and Kharif crops of Punjab, India. Proceedia Environmental Science. 3:801-808.
https://doi.org/10.1016/j.proenv.2016.07.096
Sienkiewicz, Z., Calderon,C., Kerry, A.B., Addison, D., Gavarrd, A. Louise, L. and Maslanyj,M.(2017). Are Exposure to Multiple Frequencies the Key to Future Radiofrequency Research?. Frontiers in Public Health. 5:328
https://doi.org/10.3389/fpubh.2017.00328
Tai, X.S., Mao, W.L., Liu, G.X., Chen, T., Zhang,W., Wu, X.K., Long, H.Z., Zhang, B.G.and Zhang, Y. (2013). High Diversity of Nitrogen-fixing Bacteria in the Upper Reaches of the Heihe River, Northwestern China. Biogeosciences, 10: 5589-5600.
https://doi.org/10.5194/bg-10-5589-2013
Vummiti, D. (2015). Determination of Available Nutrients in Soil using the Agilent 4200 MP-AES. Agilent Technology Inc pp 2-3
Wick K, Heumesser C. and Schmidt, E. (2012). Groundwater Nitrate Contamination: Factors and Indicators. Journal of Environmental Management, 111(3):178-186.
https://doi.org/10.1016/j.jenvman.2012.06.030
Yari, S., Asadi, A.F., Jarrahi, A.M and Mohammadi, N.M. (2019). Biological Effects of Electromagnetic Waves with Emphasis on Radio and Microwaves. An Environmental Carcinogen, 2(1)35-41.
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