Prevalence of Multidrug Resistant Escherichia Coli In Suspected Cases of Urinary Tract Infection Among Patients Attending Ahmadu Bello University Medical Center, Zaria
DOI:
https://doi.org/10.47430/ujmr.2052.017Keywords:
Prevalence, Escherichia coli, Multi drug resistant, Urinary tract infectionAbstract
Urinary tract infections (UTIs) are a major health problem, the second most prevalent human bacterial infection after respiratory tract infection. Patient with urinary tract infection is a potential source of multi drug resistant (MDR) Escherichia coli (E. coli) with the potentials to spread antimicrobial resistant genes to other bacteria in the environment and other human populations. The aim of the study was to isolate and determine the prevalence of MDR Escherichia coli from patients suspected with urinary tract infection attending Ahmadu Bello University Medical Center, Zaria. A total of 95 urine samples were collected and processed according to standard microbiological methods for the isolation and identification of E. coli. Antibacterial susceptibility pattern of the isolates was determined using Kirby-Bauer’s disk diffusion technique as well as MAR indices. Out of the 95 urine samples collected 32 were from males and 63 were from females, whose ages were between 5 and 74 years. The results revealed that 35 (36.8%) out of the 95 samples collected were positive for E. coli with high prevalence among the female patients 23(24.2%) compared to the male patients 12(12.4%). High prevalence of E. coli was also reported among the patients within the age ranges of 15-24 and 25-34 years. The Escherichia coli isolates demonstrated high resistance to sparfloxacin (91.4%), followed by cotrimoxazole and amoxicillin (82.9%). Additionally, 30 (85.7%) of the isolates exhibited multi drug resistance and 94.3% (n = 33/35) had a MAR index above 0.2. The study demonstrated that some of the E. coli isolates in the study are from high-risk contaminated sources where there may be high frequency of antibiotic usage. Therefore, the study indicated the need for Physicians to prescribed antibiotics to patients following standard antibacterial susceptibility testing.
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References
Aboderin, O. A., Abdu, A. R., Odetoyin, B. W. and Lamikanra, A. (2009). Antimicrobial resistance in Escherichia coli strains from urinary tract infections. Journal of the national medical association, 101(12), 1268-1273.
https://doi.org/10.1016/S0027-9684(15)31138-X
Aibinu, I., Odugbemi, T., Koenig, W. and Ghebremedhin, B. (2012). Sequence type ST131 and ST10 complex (ST617) predominant among CTX-M-15- producing Escherichia coli isolates from Nigeria. Clinical Microbiology Infection 18: E49-E51.
https://doi.org/10.1111/j.1469-0691.2011.03730.x
Al-jiffri, O., Zahira, M. F., El-Sayed, F. and Al- Sharif, M. (2011). Urinary Tract Infection with E. coli and antibacterial activity of some plant extract. International Journal of Microbiology Research. 2:1-7.
Arredondo-Garcia, J. L. and Amabile-Cuevas,C.F. (2008). High resistance prevalence towards ampicillin, cotrimoxazole and ciprofloxacin, among uropathogenic Escherichia coli isolates in Mexico City. Journal of Infection in Developing Countries. 2:350-353.
https://doi.org/10.3855/jidc.195
Babalola, O. O. and Lamikanra, A. (2002). Pattern of antibiotic purchases in community pharmacies in South Western Nigeria. Journal of Social and AdministrativePharmacy.19:33 - 38.
Batabyal, B. and Himanshu (2018). Isolation and antimicrobial resistance patterns of Escherichia coli causing urinary tract infections in children aged 1 to 12 years. Journal of Bacteriol Infec Dis.2 (1):35-42.
Bauer, A.W., Kirby, W. M., and Sherris, J. C. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology; 45(4):493-6.
https://doi.org/10.1093/ajcp/45.4_ts.493
Bogaard, V. A. E. (2001). Antibiotic resistance of faecal Escherichia coli in poultry, poultry farmers and poultry slaughterers. Journal of Antimicrobial Chemo therapy. 47: 763-771.https://doi.org/10.1093/jac/47.6.763 PMID: 11389108
https://doi.org/10.1093/jac/47.6.763
Campbell, J. I., Nhung, N. T., Nhung, H. N., Van Minh, P. and Wagenaar, J. A. (2015). Prevalence and risk factors for carriage of antimicrobial-resistant Escherichia coli on household and small-scale chicken farms in the Mekong Delta of Vietnam. 2: 144-2152. https://doi.org/10.1093/jac/dkv053 PMID: 25755000.
https://doi.org/10.1093/jac/dkv053
Caudell, M. A., Mair, C., Subbiah, M., Matthews, L., Quinlan, R. J. and Quinlan, M. B. (2018). Articles Identification of risk factors associated with carriage of resistant Escherichia coli in three culturally diverse ethnic groups in Tanzania: a biological and socioeconomic analysis. 2: e489-e497. https:// doi.org/10.1016/S2542- 5196(18)30225-0 PMID: 30396440.
https://doi.org/10.1016/S2542-5196(18)30225-0
Cheesbrough, M. (2006). District laboratory practice in tropical countries, part 2. Cambridge university press. Pp132-234.
https://doi.org/10.1017/CBO9780511543470
Chen, X., He, L., Li, Y., Zeng, Z., Deng, Y., Liu,Y. and Liu, J. H. (2014). Complete sequence of a F2: A-: B- plasmid pHN3A11 carrying rmtB and qepA, and its dissemination in China. Vet Microbiol 174: 267-271.
https://doi.org/10.1016/j.vetmic.2014.08.023
Cho, S. H., Lim, Y. S. and Kang, Y. H. (2012).Comparison of Antimicrobial Resistance in Escherichia coli Strains Isolated from Healthy Poultry and Swine Farm Workers Using Antibiotics in Korea. Osong Public Heal Res Perspect.; 3: 151-155. https://doi.org/10.1016/j.phrp.2012.0 7.002 PMID: 24159507.
https://doi.org/10.1016/j.phrp.2012.07.002
Collee, J. G., Duguid, J. P. and Fraser, A. G. (1996). Laboratory strategy in diagnosis of infective syndromes. In: Mackie and McCartney Practical Medical Microbiology, 14th ed. London: Churchill Livingstone.:53-94.
Eghieye, M. O., Jodi, S. M., Bassey, B. E., Nkene, I. H., Abimiku, R. H. and Ngwai, Y. B. (2018). Antimicrobial Resistance Profile of Escherichia coli Isolated from Urine of Patients in Selected General Hospitals in Abuja Municipal, Nigeria. Asian Journal of Advanced Research and Reports, 2(2): 1-10,
https://doi.org/10.9734/ajarr/2018/v2i229745
Farouk, S. N., Muhammad, A., Muhammad, S.A. and Abubakar, U. Z. (2019). Prevalence and Antibiotic Susceptibility Pattern of Escherichia coli Isolated from Urine Samples of Urinary Tract Infection Patients. ARC Journal of Urology, (4)1:14-20.
https://doi.org/10.20431/2456-060X.0401004
Foxman, B., Barlow, R., D'Arcy, H., Gillespie,B. and Solbel, J. (2000). Urinary tract infection: Self-reported incidence and associated costs. Annals of Epidemiology; 10:509-515
https://doi.org/10.1016/S1047-2797(00)00072-7
Foxman, B. and Brown, P. (2003). Epidemiology of urinary tract infections: Transmission and risk factors, incidence, and costs. Infectious Disease Clinics of North America; 17:227-241
https://doi.org/10.1016/S0891-5520(03)00005-9
Frye, J. G. and Jackson, C. R. (2013). Genetic mechanisms of antimicrobial resistance identified in Salmonella enterica, Escherichia coli, and Enterococcus spp. isolated from U.S. food animals. Front Microbiology 4: 1-22.Gales, A. C., Jones, R. N., Gordon, K. A.,
https://doi.org/10.3389/fmicb.2013.00135
Sader, H. S., Wilke, W. W., Beach, M.L. and SENTRY Study Group Latin America, T. (2000). Activity and spectrum of 22 antimicrobial agents tested against urinary tract infection pathogens in hospitalized patients in Latin America: report from the second year of the SENTRY antimicrobial surveillance program (1998). Journal of Antimicrobial Chemotherapy, 45(3), 295-303.
https://doi.org/10.1093/jac/45.3.295
Gupta, K., Sahm, D. F. and Mayfield, D. (2001). Antimicrobial resistance among uropathogens that cause community- acquired urinary tract infections in women: a nationwide analysis. Clinical Infectious Diseases.33:89-94.
https://doi.org/10.1086/320880
Gupta, K. and Stamm, W. E. (2005). Urinary tract infections. In: Dale DC, Federman DD, editors. ACP Medicine. New York: WebMD. Section 7, Chap. 23.
Hima-Lerible, H., Ménard, D. and Talarmin, A. (2003). Antimicrobial resistance among uropathogens that cause community- acquired urinary tract infections in Bangui, Central African Republic. Journal of Antimicrobial Chemotheraphy.51:192-194.http://www.facm.ucl.ac.be/intranet/CLSI/CLSI-2015- M100-S25-original.pdf
https://doi.org/10.1093/jac/dkg053
Johnson, J. R., Kuskowski, M. A., O'Bryan, T. T., Colodner, R. and Raz, R. (2005). Virulence genotype and phylogenetic origin in relation to antibiotic resistance profile among Escherichia coli urine sample isolates from Israeli women with acute uncomplicated cystitis. Antimicrobial agents and chemotherapy, 49(1), 26-31.
https://doi.org/10.1128/AAC.49.1.26-31.2005
Kariuki, S., Revathi, G., Corkill, J., Kiiru, J., Mwituria, N. and Hart, C. A. (2007). Escherichia coli from community- acquired urinary tract infections resistant to fluoroquinolones and extended-spectrum beta-lactams. Journal of Infection in Developing Countries.1:257-262.
https://doi.org/10.3855/jidc.361
Kamweli, M. A., Kwaga, J., Okolocha, J., Mba,N. and Thaku, S. (2019). Prevalence and risk factors for multi-drug resistant Escherichia coli among poultry workers in the Federal Capital Territory, Abuja, Nigeria. PLoS ONE 14(11): e0225379. https://doi. org/10.1371/journal.pone.0225379.
https://doi.org/10.1371/journal.pone.0225379
Lau, S. H., Kaufmann, M. E., Livermore, D. M., Woodford, N., Willshaw, G. A., Cheasty, T., Stamper, K., Reddy, S., Cheesbrough, J., Bolton, F. J., Fox, A.J. and Upton, M. (2008). UK epidemic Escherichia coli strains A-E, with CTX- M-15 beta-lactamase, all belong to the international O25:H4-ST131 clone. J Antimicrob Chemo ther 62: 1241-1244.
https://doi.org/10.1093/jac/dkn380
Margiorakos, A. P., Srinivasan, A., Carey, R. B., Carmeli, Y. and Falagas, M. E., (2012). Multi-drug resistant, extensively drug resistant and pan drug-resistant bacteria: An international expert proposal for interim standard definition for acquired resistance. Clinical Microbiology Infection. 18(3), 268-281.
https://doi.org/10.1111/j.1469-0691.2011.03570.x
Manges, A. R., Natarajan, P., Solberg, O. D., Dietrich, P. S. and Riley, L. W. (2006). The changing prevalence of drug- resistant Escherichia coli clonal groups in a community: evidence for community outbreaks of urinary tract infections. Epidemiology & Infection, 134(2), 425-431.
https://doi.org/10.1017/S0950268805005005
Manges, A. R., Tabor, H., Tellis, P., Vincent, C., and Tellier, P. P. (2008). Endemic and epidemic lineages of Escherichia coli that cause urinary tract infections. Emerging infectious diseases, 14(10), 1575.
https://doi.org/10.3201/eid1410.080102
Namrathan, W. N. (2015). Profile of urinary tract infection and quinolone resistances among Escherichia coli and Klebsellia species isolates. International Journal of Current Microbiology and Applied Sciences.; 4(7):749-756.
Nikaido, H. (2009). Multidrug Resistance in Bacteria. Annual Review Biochemistry.; 78: 119-146. https://doi.org/10.1146/annurev.bioche m.78.082907.145923 PMID: 19231985
https://doi.org/10.1146/annurev.biochem.78.082907.145923
Ovalle, A. and Levancini, M. (2001). Urinary tract infections in pregnancy. Current Opinion in Urology.11:55-59
https://doi.org/10.1097/00042307-200101000-00008
Petty, N. K., Ben-Zakour, N. L., Stanton-Cook, M., Skippington, E., Totsika, M., Forde, B. M., Phan, M. D., Gomes, M. D., Peters,K. M., Davies, M., Rogers, B. A., Dougan, G., Rodriguez-Bano, J., Pascual, A., Pitout, J. D., Upton, M., Paterson, D. L., Walsh, T. R., Schembri, M. A. and Beatson, S. A. (2014). Global dissemination of a multidrug resistant Escherichia coli clone. Proc Natl Acad Sci U S A 111: 5694-5699.
https://doi.org/10.1073/pnas.1322678111
Philippon, A., Labia, R. and Jacoby, G. (1989). Extended spectrum beta lactamases. Journal of Antimicrob Agents Chemo.33:1131-6
https://doi.org/10.1128/AAC.33.8.1131
Rogers, B. A., Sidjabat, H. E. and Paterson, D.L. (2011). Escherichia coli O25b-ST131: a pandemic, multi resistant, community- associated strain. Journal of Antimicrobial Chemo theraphy. 66: 1-14.
https://doi.org/10.1093/jac/dkq415
Ruiz, J., Simon, K., Horcajada, J. P., Velasco, M., Barranco, M., Roig, G., Moreno- Martinez, A., Martinez, J.A., Jimenez, de Anta T., Mensa, J. and Vila, J. (2002). Differences in virulence factors among clinical isolates of Escherichia coli causing cystitis and pyelonephritis in women and prostatitisin men. Journal of Clinical Microbiology; 40:4445-4449.
https://doi.org/10.1128/JCM.40.12.4445-4449.2002
Sabir, S., Anjum, A. A., Ijaz, T., Ali, M. A., Khan, M. R. and Nawaz, M. (2014). Isolation and antibiotic susceptibility of E. coli from urinary tract infections in a tertiary care hospital. Pak Journal of Medical Science; 30 (2):389-392.
https://doi.org/10.12669/pjms.302.4289
Sire, J. M., Nabeth, P. and Perrier-Gros-Claude,J. D. (2007). Antimicrobial resistance in outpatient Escherichia coli urinary isolates in Dakar, Senegal. Journal of infection in Developing Countries.1:263- 268.
https://doi.org/10.3855/jidc.362
Sotto, A., De Boever, C. M. and Fabbro-Peray,P. (2001). Risk Factors for Antibiotic- resistant Escherichia coli isolated from hospitalized patients with urinary tract infections: a prospective study. Journal of Clinical Microbiology.39:438-444.
https://doi.org/10.1128/JCM.39.2.438-444.2001
Swedres- Svarm (2014). Consumption of antibiotics and occurrence of antibiotic resistance in
Sweden.2014.http://www.sva.se/globala ssets/redesign2011/pdf/om_sva/publikati oner/swedres_svarm2014.pdf. Accessed 8June 2017.
Szmolka, A. and Nagy, B. (2013). Multidrug resistant commensal Escherichia coli in animals and its impact for public health. Front Microbiology. 4:258. https://doi.org/10.3389/fmicb.2013.0025 8 PMID: 24027562
https://doi.org/10.3389/fmicb.2013.00258
Thenmozhi, S., Rajeswari, P., Suresh, K. B. T., Saipriyanga, V. and Kalpana, M. (2014). Multi-Drug Resistant Patterns of Biofilm Forming Aeromonas Hydrophila from Urine Samples International Journal of Pharmaceutical Sciences and Research. In: Intentional Journal of Pharmaceutical Sciences Research. 5(7): pp. 2908-2918.
[Internet]. [cited 5 Jul 2019]. Available: http://ijpsr.com/bft-article/multi-drug- resistant-patterns-of-biofilm- formingaeromonas-hydrophila-from-urine- samples/?view=fulltext
Walsh, C. (2003). Where will new antibiotics come from? Nat Rev Microbiol 1: 65-70.
https://doi.org/10.1038/nrmicro727
Warren, J. W., Abrutyn, E., Hebel, J. R., Johnson, J. R., Schaeffer, A. J. and Stamm, W. E. (1999). Guidelines for antimicrobial treatment of uncomplicated acute bacterial cystitis and acute pyelonephritis in women. Clinical Infectious Diseases, 29(4), 745-759.
https://doi.org/10.1086/520427
Wenzel, R. P., Bearman, G. and Edmond, M. B. (2008). Screening for MRSA: a flawed hospital infection control intervention. Infectious Control Hospital Epidemiology 29: 1012-1018.
https://doi.org/10.1086/593120
Williams, N. S., Bulstrode, C. J. K. and O'Connell, P. R. O. (2008), editors. 25th ed. London: United Kingdom: Edward Arnold Publishers; Bailey & Love's Short Practice of Surgery.;1329-30.
https://doi.org/10.1111/j.1468-5906.2008.00400.x
https://doi.org/10.1111/j.1468-5906.2008.00385.x
Williamson, D. A., Lim, A., Wiles, S., Roberts, S. A. and Freeman, J. T. (2013). Population-based incidence and comparative demographics of community- associated and healthcare-associated Escherichia coli bloodstream infection in Auckland, New Zealand, 2005-2011. BMC Infect Dis 13: 385-392.
https://doi.org/10.1186/1471-2334-13-385
Zhang, L. and Foxman, B. (2003). Molecular epidemiology of Escherichia coli mediated urinary tract infections. Frontiers in Bioscience;8: e235-e244.
Zhuge, X., Ji, Y., Tang, F., Sun, Y., Jiang, M. and Hu, W. (2017). Population Structure and Antimicrobial Resistance Traits of Avian-origin. PMID: 31059196.
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