Evaluation of Susceptibility Profiles of Some Bacteria to Flouroquinolones in Kano Metropolis, Nigeria

Authors

  • Mohammed, B. Department of Microbiology, Bayero University, Kano. P. M. B. 3011
  • Ahmad, R. H. Department of Microbiology, Bayero University, Kano. P. M. B. 3011

DOI:

https://doi.org/10.47430/ujmr.2051.005

Keywords:

flouroquinolones,, clinical bacterial isolates,, sensitivity and resistance profiles.

Abstract

Resistance to broad spectrum antibiotics such as flouroquinolones by bacteria is becoming a major threats towards the chemotherapy of some common pathogenic bacterial infections in the world especially the developing nations. In view of that, susceptibility profiles of some pathogenic bacteria to some flouroquinolones were evaluated using modified Kirby-Bauer Disc Diffusion Technique. Four flouroquinolones antibiotics; ofloxacin and ciprofloxacin (second generation), levofloxacin (third generation), moxifloxacin (fourth generation) were tested on five bacterial species (Staphyllococcusaureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis and Klebsiella pneumoniae) sourced from Aminu Kano Teaching Hospital, Kano, Nigeria, and re-confirmed at Microbiology Laboratory in Bayero University, Kano. The results of the study revealed that 8(26.7%) of E. faecalis isolates were sensitive levofloxacin, 6(20.0%) were saensitive to ciprofloxacin and 1(3.33%) were sensitive to ofloxacin, while 0(0.00%) was sensitive to moxifloxacin. Seven isolates (23.3%) of S. aureus were sensitive to levofloxacin, 15(50.0%) were sensitive to ciprofloxacin and 4(13.3%) were sensitive to ofloxacin, while none (100%) were sensitive to moxifloxacin. E. coli isolates were 96.6% resistant to levofloxacin, Klebsiella pneumoniae and P. aeruginosa isolates exhibited 76.6 and 50.0% resistance to the antibiotic respectively. There was significant difference in the performance of the four antibiotics used, ciprofloxacin better than others (p-value 0.00604). There was no significant difference in the percentages of sensitive bacterial species to the antibiotics used in the research (p-value 0.614), however, P. aeruginosa showed relatively higher number of sensitive isolates 30(40%). It can be concluded that 58% of the isolates were moderately sensitive to all the antibiotics in this study.

Downloads

Download data is not yet available.

References

Alkali, B., Iliyasu, G., Adamu, A. A., Abdurrazak, K., Muhammad, H. A., Faruk, S. and Agwu, E. (2019). Superbugs -Related Prolonged Admissions in Three Tertiarry Hospitals in Kano State, Nigeria. Pan African Journal of Medicine, 32 (106):100

https://doi.org/10.11604/pamj.2019.32.166.18481

Alekshun, M.N. and Levy, S.B. (2007).Mechanism, OF Antibacterial Multidrug Resistance. Cell 128; 1037 -1050.

https://doi.org/10.1016/j.cell.2007.03.004

Arora, D.R. and Arora, B.B. (2014).Textbook of Microbiology Fourth Edition, CBS Publishers and distributors PVT. 4D New Delhi, India. Pp. 218 - 220.

Bonke, R., Wacheck, S., Bumann, C. Thum, C. Stuber, E. Konig, M. Stephen, R., Fredriksson- Ahomaa, M. (2012). High Prevalence of Salmonella enteric subsydiarizone in tonsils of Sheep at Saughter.Food Research International 45(2), 880 - 884.

https://doi.org/10.1016/j.foodres.2011.01.050

Cheesbrough, M. (2006).District Laboratory Practice in Tropical Countries, Part 2, Published by Cambridge University Press, the Edinburg Building, Cambridge. Pp. 62 - 70.

https://doi.org/10.1017/CBO9780511543470

Criedrys-Kalemba, S. and Bilska, I. (1993). Analysis of Susceptibility of Bacteria Isolated from Clinical meternalbetween 1989 - 1991 to Ofloxacin. Med. Dosw. Mikrobial 43(1), 119.

Depinna, E.Threlfall, J. Levent, B., Hopkins,

K.L. Ward R., and Derek, J. (2005). Multidrug - Resistant Salmonella java Emerging Infection Disease, 11(1), 170.

https://doi.org/10.3201/eid1101.031092

Fred, C.T. (2006). Mechanisms of Antimicrobial Resistance in Bacteria.American Journal of Infection Control, 34 (5) 53 -510.

https://doi.org/10.1016/j.ajic.2006.05.219

Hooper (2001), Threlfall and Hopkins, 2005), Tenover (2006) and Alekshun and Levy (2007).

Gita, S., Tushar, A., Vishal, J. and Niranjan, N. (2012).Moxyloxacin Resistance, Intrinsic to Antibiotic or Related to mutation.American Academy of Optometry, 89(12). 1721 - 1724.

https://doi.org/10.1097/OPX.0b013e31827717d4

Hooper, D.C. (2003).Emerging mechanisms of Flouronolone and Resistance Emerg. Infect. Dis. 7(2), 337 - 41.

https://doi.org/10.3201/eid0702.010239

Hopkins, K.L. Davies, R.H. and Threlfall, E.J. (2005).Mechanisms of Quinolones Resistance in E. coli and Salmonella Recent Developments.International Journal of Antimicrobial Agents, 25(5), 358 - 373.

https://doi.org/10.1016/j.ijantimicag.2005.02.006

Jo, M. (2018).When Antibiotics Turn Toxic. Researchers are Asking Why Flouroquinolone Drugs Cause Rare Disabling Side Effects. Nature, 555(431):18481.

https://doi.org/10.1038/d41586-018-03267-5

Kato, J.I. Nishmura, Y. Imamura, R., Niki, H., Jiraga, S., Suzuki, H. (1990).New Topoisomerase Essential for Chromesome Segregation in E. coli. Cell, 63, 393 - 404.

https://doi.org/10.1016/0092-8674(90)90172-B

Oliphant, C.M. and Eray, M.G. (2002) Quinolones, a Comprehensive Review, AM. Fam. Physician , 65 (3): 455 - 64.

Reis, A.C.C. Santos, S.R.D. de Souze, S.C. Saldanha, M.C., Pitanga, V.P. Olveira,R.R. (2016). Ciprofloxacin Resistance Pattern among Bacteria Isolated from Patients with Community Acquired Urinary Text Infection. Rev. Inst. Med. Trop. Saopaulo, 58, 53.

https://doi.org/10.1590/S1678-9946201658053

Shamsuddeen, U. Abdulkadir, R.S, and Bassey,E. (2013).Incidence of various Vibrio Species in water from Different Source in Ja'en, Kano State of Nigeria. International Journal of Agriculture and Bioscience, 2 (5): 192-195.

Siegrist, H.H. Nepa, M.C. Jacquent, A. (1999). Susceptibility to Levofloxacin of Clinical Isolates of Bacteria from Intensive Care and Haematology/oncology Patients in Switzerland. A Multicentre Study. Journal of Antimicrobial Chemother43 (Supp. C, 51 - 4).

https://doi.org/10.1093/jac/43.suppl_3.51

Tenover, F.C. (2006). Mechanisms of Antimicrobial Resistance in Bacteria. Am. J. Med, 119 (6 Suppl. 1): 53 - 70.

https://doi.org/10.1016/j.amjmed.2006.03.011

Valery, N. C., Emiliya, V. N., Gabna, N. L. and Oleg, N. C. (2014). Flouroquinolones: Synthesis and Application . Fluorine in Heterocyclic Chemistry, 2;111-179.

https://doi.org/10.1007/978-3-319-04435-4_3

Willey, J.M. Sherwood, L. M. AND Woolverton,C.J. (2008). Prescott, Harley and Klein's Microbiology Seventh Edition, Published by McGraw-Hill 1221 Avenue, New York, NY10020. Pp. 847 - 849.

Downloads

Published

30-06-2020

How to Cite

Mohammed, B., & Ahmad, R. H. (2020). Evaluation of Susceptibility Profiles of Some Bacteria to Flouroquinolones in Kano Metropolis, Nigeria. UMYU Journal of Microbiology Research (UJMR), 5(1), 31–35. https://doi.org/10.47430/ujmr.2051.005