Isolation and Antibiogram of Staphylococcus aureus from Powdered Milk Sold in Samaru Market, Zaria

Authors

  • Madika, A. Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria-Nigeria
  • Musa, B., Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria-Nigeria
  • Sulaiman, M.A. Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria-Nigeria
  • Hussaini, I.M. Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria-Nigeria
  • Jimmy, G.G. Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria-Nigeria

DOI:

https://doi.org/10.47430/ujmr.2051.012

Keywords:

Isolation, Powdered milk, Staphylococcus aureus, Antibiogram, Multiple antibiotic resistance index (MARI)

Abstract

Milk is a nutritive food that serves as an excellent growth medium for wide variety of microbes. This study was conducted to determine the occurrence of Staphylococcus aureus in powdered milk sold in Samaru market, Zaria, and their antibiotic susceptibility patterns. Fifty (50) powdered milk samples were collected from different shop vendors. The samples were used for isolation of Staphylococcus aureus by cultural method. Staphylococcal count was determined and the colonies observed were identified based on colonial morphology, microscopic and biochemical characterization. The antibiogram of the isolates were also determined using Kirby-Bauer disc diffusion method. The highest Staphylococcal mean count of 1.47x104 CFU/g was observed in powdered milk samples displayed outside the shop in sacks and the lowest count of 1.22x104 CFU/g was observed for samples displayed inside the shop in sacks. Fifteen (15) samples were positive for Staphylococcus aureus giving a percentage occurrence of 30%, with samples displayed outside the shop in sacks having the highest occurrence (42.9%) while the least (16.67%) occurrence was observed in those displayed inside the shop in sacks. The antibiogram revealed a high susceptibility to Ciprofloxacin (100%), Gentamicin (93.33%), Erythromycin and Chloramphenicol (73.33%). While other isolates showed high resistance to Norfloxacin (66.67%) and Ampicillin-cloxacillin (26.67%). Five (5) isolates were resistant to multiple antibiotics with multiple antibiotic resistance index (MARI) ranging from 0.3-0.4. The study revealed high occurrence of Staphylococcus aureus in powdered milk and its resistance to commonly used antibiotics possess a public health risk to consumers of these products

 

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References

Aboh, E.A., Giwa, F.J. and Giwa, A. (2015). Microbiological assessment of well waters in Samaru, Zaria, Kaduna State, Nigeria. Annals of African Medicine,14(1): 32-38

https://doi.org/10.4103/1596-3519.148732

Afroz, H., Sultana, F., Fakruddin, M., Kamrunnahar, Khan, Z.U.M. and Datta,S. (2013). Isolation of Escherichia coli and Staphylococcus aureus from full cream powder milk sold under market conditions at Dhaka, Bangladesh and their antibiotic susceptibility. Journal of Advanced Scientific Research, 4(3):27-31

https://doi.org/10.12980/APJTB.4.2014B85

Agboke, A.A., Osonwa, U.E., Opurum, C.C. and Ibezim, E.C. (2011). Evaluation of microbiology quality of some soybean milk products consumed in Nigeria. Prime Research on Medicine, 1(2):25-30

https://doi.org/10.4314/jpb.v8i1.6

Ahmed, S. and Anwar, M.N. (2006). Microbial Contaminants of Dried Powder Milk Available in Local Markets of Bangladesh. Bangladesh Journal of Microbiology, 23(2):162- 164

https://doi.org/10.3329/bjm.v23i2.885

Ateba, C.N., Mbewe, M., Moneoang, M.S. and Bezuidenhout, C.C. (2010). Antibiotic- resistant Staphylococcus aureus isolated from milk in the Mafikeng Area, North West province, South Africa.South African Journal of Science,106(11/12):1-6

https://doi.org/10.4102/sajs.v106i11/12.243

Ayele, Y., Gutema, F.D., Edao, B.M., Girma, R.,Tufa, T.B., Beyene, T.J., Tadesse, F., Geloye, M. and Beyi, A.F. (2017). Assessment of Staphylococcus aureus along milk value chain and its public health importance in Sebeta, central Oromia, Ethiopia. BMC Microbiology, 17:141DOI 10.1186/s12866-017-1048-9

https://doi.org/10.1186/s12866-017-1048-9

Bashar, T. and Malek, M.A. (2006). Prevalence of microbial contaminants of some milk and milk products available in different markets of Dhaka. Bangladesh Journal of Microbiology, 23(1): 75-77

Cheesbrough, M. (2009). District Laboratory Practices in Tropical Countries. Part two, 2 Ed., Cambridge University Press, United Kingdom

Clinical and Laboratory Standards Institute (CLSI) (2015). Performance standardsfor antimicrobial susceptibility testing; twenty-fifth informational supplement, M100-S25, 35(3): 64-71

Foods Standards Australia New Zealand (2018). Compendium of Microbiological Criteria for Food.P10https://www.foodstandards.g ov.au/publications/Documents/Comped ium%20of20Microbiological%20Criteria/ Compendium revised-Jan-2018.pdf. Accessed on 29th October, 2019

Furtula, V.,Jackson, C.R., Farell, E.G., Barrett, J.B.,Hiott, L.M. and Chambers, P.A. (2013).Antimicrobial Resistance in Enterococcus spp.Isolated from Environmental Samples in anArea of Intensive Poultry Production. International Journal of Environmental Research and Public Health, 10:1020- 1036

https://doi.org/10.3390/ijerph10031020

Le Loir, Y., Baron, F., and Gautier, M. (2003). Staphylococcus aureus and food poisoning. Genetics and Molecular Research, 2(1): 63-76

Mailard, J. Y. (2002). Bacterial target sites for biocideaction. Journal of Applied Microbiology, 82:53-60

Okpo, N.O., Abdullahi, I.O., Whong, C.M.Z. and Ameh, J.B. (2016). Occurrence and antibiogram of Staphylococcus aureusin dairy products consumed in parts of Kaduna state, Nigeria. Bayero Journal of Pure and Applied Sciences, 9(2): 225- 229

https://doi.org/10.4314/bajopas.v9i2.39

Poole, K. (2002). Mechanism of Bacterial Biocide and Antibiotic Resistance. Journal of Applied Microbiology,92: 55-64

https://doi.org/10.1046/j.1365-2672.92.5s1.8.x

Van Kessel, J.S., Karns, J. S., Gorski, L., McCluskey, B. J. and Perdue, M. L. (2004). Prevalence of Salmonellae, Listeria monocytogenes, and Fecal Coliforms in Bulk Tank Milk on US Dairies. Journal of Dairy Science,87:2822-2830

https://doi.org/10.3168/jds.S0022-0302(04)73410-4

Wouters, J.T.M., Ayad, E.H.E., Hugenholtz, J. and Smit, G. (2002). Microbesfrom raw milk for fermented dairy products. International Dairy Journal, 12(2-3):91- 109

https://doi.org/10.1016/S0958-6946(01)00151-0

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Published

30-06-2020

How to Cite

Madika, A., Musa, B., Sulaiman, M.A., Hussaini, I.M., & Jimmy, G.G. (2020). Isolation and Antibiogram of Staphylococcus aureus from Powdered Milk Sold in Samaru Market, Zaria. UMYU Journal of Microbiology Research (UJMR), 5(1), 77–81. https://doi.org/10.47430/ujmr.2051.012

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