In Vivo Evaluation of Acute and Subacute Toxicity of Jatropha curcas Seed Oil
DOI:
https://doi.org/10.47430/ujmr.2381.018Keywords:
In vivo, Jatropha curcas, seed oil, toxicityAbstract
Medicinal plants are regarded as safe because of their natural origin, nevertheless, they can contain toxic substances that can exert adverse effects. This study aims to evaluate acute and subacute toxicity of Jatropha curcas seed oil using modified Lorke method and 28 days repeated dosing of grouped rats with normal saline and -10, 300, 600 mg/kg body weight of the seed oil. At the end of the experimentation, haematological and biochemical analysis of blood samples and the histopathology of the liver and kidney of rats in each group were evaluated. The Lethal Dose (LD50) of J. curcas seed oil was lower than 5000mg/kg b.wt. Haematological and biochemical analysis showed a dose-dependent decrease in the Hemoglobin, Packed Cell Volume and Red Blood Cells, an increase in the level of Total White Blood Count and Platelet Count, Aspartate aminotransferase, Alanine aminotransferase, Alkaline phosphatase, urea and creatinine in the treated groups, while the histological evaluation revealed distortion in liver and kidney cytoarchitecture of rats administered with 600 mg/kg b.wt of the seed oil. The oil was found less toxic at the acute phase but there was toxicity manifestation in subacute phase causing adverse effects on haematological, biochemical parameters and the tissues of the kidney and liver. The study suggests that the seed oil can be used, but caution should be exercised when using it at high doses for prolonged periods.
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Abdel Aziz, A. D., Sayed, A. A., Ahmed, A. H. and Dalia M. M. S. (2014). The ameliorative effect of L-carnitine on experimentally induced liver cirrhosis in male albino. Journal of American Science, 10, (11), 8-18. http://www.jofamericanscience.org.
Abdel-safy, S., Nasr, S., Abdel, R., and Habeeb, S. (2011). Effect of various levels of dietary Jatropha curcas seed meal on rabbits infested by the adult ticks of Hyalomma marginatum I. animal performance, anti-tick feeding and haemogram. Tropical Animal Health Production, 43, 347https://doi.org/10.1007/s11250-010-9696-x
Abdul, W. M, Hajrah, N. H., Sabir, J. S., Al-Garni, S. M., Sabir, M. J., Kabli, S. A., Saini, K. S. and Bora, R. S. (2018). Therapeutic role of Ricinus communis L. and its bioactive compounds in disease prevention and treatment. Asian Pacific Journal of Tropical Medicine, 11, 177-85. Retrieved from http://www.apjtm.org/text.asp?2018/11/3/177/228431https://doi.org/10.4103/1995-7645.228431
Agbogidi, O. M., Akparobi, S. O. and Eruotor, P. G. (2013). Health and environmental benefits of Jatropha curcas Linn. Journal of Applied Science Rep, 1(2), 36-39
Arsad, S., Esa, N, Hamzah, H., and Othman, F. (2013). Evaluation of acute, subacute and subchronic oral toxicity of Rhaphidophora decursiva (Roxb.) schott extract in male Sprague dawley rats. Journal of Medicinal Plant Research, 7, 3030-3040
Asare, G. A., Bugyei, K., Sittie, A., Yahaya, E. S., Gyan, B., Adjei, S., Addo, P., Wiredu, E. K., Adjei, D. N., and Nyark, A. K. (2012). Genotoxicity, cytotoxicity and toxicological evaluation of whole plant extracts of the medicinal plant Phyllanthus niruri (Phyllanthaceae). Genetics and Molecular Research, 11 (1), 100-111. doi: 10.4238/2012https://doi.org/10.4238/2012.January.13.3
Asep, S., Hening, H., Gema, S. P., Gigih, S., Widya, M. C., and Sahidin, S. (2017). Anticancer activity of jatrophone an isolated compound from Jatropha gossypifolia plant against hepatocellular cancer cell Hep G2 1886. Biomedical Pharmacology Journal, 10(2), 8-11https://doi.org/10.13005/bpj/1154
Awasthy, V., Vadlamudi, V., Koley, K., Awasthy, B. and Singh, P. (2010). Biochemical changes after short-term oral exposure of Jatropha curcas seeds in Wistar rats. Toxicology international, 17, 67-70https://doi.org/10.4103/0971-6580.72673
Awotunde, O. S., Adewoye, S. O., Dhanabal, P. S. and Hawumbad, J. (2019). Subacute toxicity study of aqueous root extract of Terminalia schimperiana in male Wistar rats. Toxicology Reports, 6: 825-832.https://doi.org/10.1016/j.toxrep.2019.07.006
Azuibike, C. N., Mitchell, N. C., Okwuosa, P. U. Achukwu, T. C. and Maduka, O. C. (2015). Acute toxicity and histopathological Effects of crude aqueous extract of Jatropha curcas leaves in mice. Journal of Medicinal Plant, 9(7), 340-346.https://doi.org/10.3923/rjmp.2015.340.346
Ciappin, A. L., Ferreira, A. F., Pereira, R. I., Sousa, R. T., Matos, S. F., Melo-Reis, R. P., Gonçalves, J. P., Cardoso Bailão, E. F. and Almeida, M. L. (2017). Toxicity of Jatropha curcas L. latex in Allium cepa. Bioscience Journal, 33(5), 1295-1304.https://doi.org/10.14393/BJ-v33n5a2017-33835
Cotter, S. M. (2022). Red blood cells in animals. Retrieved from https://www.msdvetmanual.com/circulatory-system/hematopoietic-system introduction/red-blood-cells-in-animals
Dada, E. O., Ekundayo, F. O. and Makanjuola, O. O. (2014). Antibacterial activities of Jatropha curcas (Linn) on coliforms isolated from surface waters in Akure, Nigeria. International Journal of Biomedical Science, 10 (1), 25-30
Daniyan, S. Y., Abalaka, M. E., Elemba, O. M. and Aransiola, M. E. (2011). In vitro antimicrobial activities and phytochemical screening of Jatropha curcas seed extract. International Research Journal of Pharmacy 2 (7), 60-64. Retrieved from http://www.irjponline.com
Emejulu, A. A., Alisi, C. S., Onwuliri, V. A. and Awurum, A. P. (2017). Effect of ethanol leaf extract of Jatropha curcas on some liver and kidney function biomarkers of normal rabbits. Futo Journal Series (FUTOJNLS), 3(2), 213 - 224. Retrieved from http://www.futojnls.orgd
Fábio, S. M., Angelina, L. C., Ednaldo, C. R. and Luciane, M. A. (2017). Factors that influence in Jatropha curcas L. latex production. Bragantia, 77 (11), 4-8.https://doi.org/10.1590/1678-4499.2016468
Foy, B. H., Sundt, T. M., Carlson, J. C. T., Aaron, D. A. and John, M. H. (2022). Human acute inflammatory recovery is defined by co-regulatory dynamics of white blood cell and platelet populations. Nature Communications 13, 4705.https://doi.org/10.1038/s41467-022-32222-2
Gadir, A., Onsa, T. O., Ali, W. E. M., El Badwi, S. M., Adam, S. E. I. (2003). Comparative toxicity of Croton macrostachys, Jatropha curcas and Piper abyssinica seeds in Nubian goats. Small Ruminant Research 48, 61-67https://doi.org/10.1016/S0921-4488(02)00181-5
Gupta, A., Kumar, A., Agarwal, A., Osawa, M. and Verma, A. (2016). Acute accidental mass poisoning by Jatropha curcas in Agra, North India. Egyptian Journal of Forensic Sciences, 6(4) ,496-500https://doi.org/10.1016/j.ejfs.2016.04.002
Hadi, T., Jalal, H., Mohammad, R. P., Hannaneh, G. and Hossain, K. T. (2019). Evaluation of the acute and sub-acute toxicity of the black caraway seed essential oil in Wistar rats. Toxicology Reports 6, 869-874. Retrieved from www.elsevier.com/locate/toxrephttps://doi.org/10.1016/j.toxrep.2019.08.010
Kuhn, V., Diederich, L. T. C., Keller, S., Kramer, C. M., Lückstädt, W., Panknin, C., Suvorava, T., Isakson, B. E., Kelm, M. and Cortese-Krott, M. M. (2017). Red Blood Cell Function and Dysfunction: Redox Regulation, Nitric Oxide Metabolism, Anemia. Antioxidants and Redox Signaling, 26(13), 718-742. doi: 10.1089/ars.2016.6954https://doi.org/10.1089/ars.2016.6954
Kumar, V., Singh, S.P., Shukla, A. and Chaudhary, J.K. (2014). Evaluation of subacute toxicity of Jatropha curcas seeds and seed oil toxicity in rats. Indian Journal of Veterinary Pathology, 38(2), 88-93https://doi.org/10.5958/0973-970X.2014.01145.6
Mahe, A., Muhammad, Y. Y., Ibrahim, A., Muhammad, A., Adam, I. K., Atiku, M. K. and Imam, A. A. (2017). In vivo and in vitro toxicity studies of crude and partially purified leaf extracts of Jatropha curcas in Wistar Albino Rats. Journal of Pharmaceutical Research International ,20(2), 1-16. doi:10.9734/JPRI/2017/38137https://doi.org/10.9734/JPRI/2017/38137
Manal, A. H., Muastafa, A. K., and Ayyad, W. A. (2018). Study the Acute & Sub Acute toxicity of Ricinus communis Linn. ethanol extract of seed in Albino mice. International Journal of Scientific Research and Management (IJSRM), 6(2), 37-45. doi: 10.18535/ijsrm/v6i2.mp03https://doi.org/10.18535/ijsrm/v6i2.mp03
Million, L., Abay, M., Solomon, A. M., Wondwossen, E., and Bekeshon, G. (2019). Acute and subacute toxicity of methanol extract of Syzygium guineense leaves on the histology of liver and kidney and biochemical compositions of blood in rats. Evidence-Based Complementary and Alternative Medicine, Article ID 5702159. doi: 10.1155/2019/570215https://doi.org/10.1155/2019/5702159
Mohammed, B.S and Awatif, A.M. (2018). Antibacterial activity and fatty acid composition of sudanese castor bean (Ricinus communis L) seed oil. Arabian Journal of Medicinal and Aromatic Plants, 4(1), 1-8. Retrieved from http://www.ajmap.info
Moshobane, M. C., Wium, C. and Mokgola, L. V. (2017). Acute poisoning in children from Jatropha curcas seeds. South African Journal of Child Health, 11(3),149-150. doi:10.7196/SAJCH.2017.v11i3.1401https://doi.org/10.7196/SAJCH.2017.v11i3.1401
Muhammad, B. Y., Alhassan, A. J and Jaafaru, I. J. (2015). Toxicity study of Ricinus communis Linn seed suspension in female Wistar Albino rats. International Journal of Biochemistry Research and Review, 7(3), 139-147. doi:10.9734/IJBcRR/2015/17511https://doi.org/10.9734/IJBCRR/2015/17511
Nwankpa, P., Ekweogu, C. N., Egwurugwu, J. N., Chukwuemeka, O. G. and Etteh, C. C. (2018). Assessment of kidney function indices in male Albino Wistar rats administered ethanol stem extract of Dennettia tripetala (pepper fruit). Biochemistry and Pharmacology, 7, 242. doi: 10.4172/2167-0501.1000242https://doi.org/10.4172/2167-0501.1000242
Olapeju, A. O., Amao, H. A., Ernesto, F. O., Taglialatelu, S., Heinz, S. C. and Werner, M. E. G. (2011). Cytotoxicity activity of crude extracts as well as pure components from Jatropha plant species used extensively in traditional medicine. Evidence-Based Complementary and Alternative Medicine, Article ID 134954. doi: 10.1155/2011/134954https://doi.org/10.1155/2011/134954
Roger Blench. (2013). Nupe Trees and Plants: Their Names and Use. Retrieved from http://www.rogerblench.info
Saganuwan, A. S. (2019). Effects of therapeutic and toxic agents on erythrocytes of different species of animals. In Anil Tombak (Eds.), Erythrocyte. Intechopen Limited. doi:105772/intechopen.85865
Sawadogo, S., Sanou, S. D., Dabiré, A. P., Belemtougri G. R., Sawadogo L., Leiris, J. de, Tanguy, S., and Boucher, F. (2018). In vivo evaluation of Jatropha curcas Linn (Euphorbiaceae) leaves acute and subacute toxicity in mice. Journal of Scientific Research, 10 (2), 187-193. doi: http://dx.doi.org/10.3229/jsr.v10i2.35267https://doi.org/10.3329/jsr.v10i2.35267
Shukla, A. and Singh, S. (2013) Evaluation and development of therapeutic management of subacute toxicity of Jatropha curcas seed oil in goats. Veterinary World, 6(11), 852-856. doi: 10.14202/vetworld.2013.852-856https://doi.org/10.14202/vetworld.2013.852-856
Shukla, A., Singh, S. P. and Tiwari, S. (2015). Transformation of toxic potential of Jatropha curcas (Ratanjyot) into protein source: A mini-review. Journal of Advanced Veterinary and Animal Research, 2(2), 89-94. doi: 10.5455/javar.2015.b82https://doi.org/10.5455/javar.2015.b82
Sreelakshmi,M. S., Abraham, J. M., Nair, N. D., Manomohan, C. B., Usha, P. T. A. and Vishnurahav, R. B. (2017). Effect of salt and lime treated Jatropha (Jatropha curcas) deoiled seed cake on growth and haematological parameters of broiler chicken. The Indian Veterinary Journal, 94 (12), 40-42
Temesgen, B. G. (2016). Chemical composition, bio-diesel potential and uses of Jatropha curcas L. (Euphorbiaceae). American Journal of Agriculture and Forestry, 4 (2), 35-48https://doi.org/10.11648/j.ajaf.20160402.15
Ting, R. P., Casas, E. V., Peralta, E. K. and Elauria, J. C. (2012). Design, fabrication and optimization of Jatropha sheller. International Journal of Optimization and Control: Theories and Application, 2(2), 113-127https://doi.org/10.11121/ijocta.01.2012.0075
Warra, A. A. and Abubakar, A. (2015). GC-MS analysis of hexane extract of Jatropha Curcas L. seed oil. World Journal of Biomedicine and Pharmaceutical Sciences, 1: 15-21
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