Studies on Bioethanol Production from Rice Stalk using Co-cultures of Aspergillus niger and Saccharomyces cerevisiae

Charanchi, A. S; Ado, S. A.; Ameh, J. B.; Musa, B; Hussaini, I. M.

doi:10.47430/ujmr.1832.014

Authors

Charanchi, A. S Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria.
Ado, S. A. Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria.
Ameh, J. B. Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria.
Musa, B Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria.
Hussaini, I. M. Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria.

DOI:

https://doi.org/10.47430/ujmr.1832.014

Keywords:

Aspergillus niger, Bioethanol, Hydrogen peroxide, Rice stalk, Saccharomyces cerevisiae, Sulfuric acid

Abstract

Bioethanol production from lignocellulosics as an alternative automobile and engine fuelhas received a considerable attention from researchers worldwide. In the current work, Bioethanol was produced from sulfuric acid and alkaline hydrogen peroxide treated rice stalk employing simultaneous saccharification and fermentation process using co-cultures of isolated and characterized strains of A. niger and S. cerevisiae. The proximate composition of the substrate was determined following standard procedures described by Association of Official Analytical Chemist. The composition of the substrate treated with sulfuric acid was moisture (4.95%), ash (4.75%), fats (4.50%),protein (5.25%), fibre (50.90%),carbohydrates (80.55%), while that of alkaline peroxide-treatment was moisture (3.65%),ash (5.10%), fats (6.60%),protein (7.00%), fibre (38.65%) andcarbohydrates (77.65%). At optimal fermentation conditions of 35ºC temperature, 5.0 pH, 4% substrate concentration, 300rpm agitation rate and 4 days fermentation period and after determining the quantity of the ethanol produced using specific gravity method, a maximum of 5.06g/100ml and 3.91 g/100ml of ethanol was obtained from sulfuric acid and hydrogen peroxide treated rice stalk respectively. The qualitative analysis using FTIR-Spectrophotometry shows the absorbance peaks of the ethanol functional groups from all the ethanol samples produced and the functional groups had their absorption peaks within their normal ranges of 3100-3600cm^-1, 2800-3000cm^-1 and 1600-1675cm^-1 for hydroxyl, alkane and alkene functional groups respectively.

Downloads

Download data is not yet available.

References

A. O. A. C (2010). Official Methods of Analysis of Chemistry, (18th Ed.) Washington, D.C. Association of Official Analytical Chemists, 10-12 pp.

Ado, S. A., Olutokun, G. B., Ameh, J. B., Yabaya, A. (2010). Bioconversion of cassava starch to ethanol in a simultaneous saccharification and fermentation process by cocultures of Aspergillus nigerand Saccharomyces cerevisiae. Science World Journal, 4(1): 19-22.

https://doi.org/10.4314/swj.v4i1.51832

Aiyejagbara, M. O. (2015). Production of bioethanol from elephant grass (pennisetum purpureum) stem. Unpublished M.Sc. thesis, Ahmadu Bello University, Zaria, 117 pp.

Ameh, J. B., Okagbue, R. N. and Ahmad, A. A. (1989). Isolation and characterization of localyeast strains for ethanol production.NigerianJournal of Technology Research,1:47-52.

Arifa T., Madiha A., and Tasnim F. (2010). Effect of cultural conditions on ethanol production by locally isolated saccharomyces cerevisiae bio-07. Jounal of Applied Pharmaceutics, 3(2): 72-78.

https://doi.org/10.21065/19204159.2.72

Balat, M., Balat, H. and Öz, C. (2008). Progress in bioethanol processing. Progress in Energy and Combustion Science, 34: 551-573.

https://doi.org/10.1016/j.pecs.2007.11.001

Carlo, R. C., Richard, S., Nazim, C. and David, B. L. (2008). Third generation biofuels via directcellulose fermentation "a review". International Journal of Molecular Sciences, 9: 1342-1360.

https://doi.org/10.3390/ijms9071342

Emeka E. I. (2001). Essential principles of physics. Eric education consult and publishers. Revised edition, 13-19 pp.

Feldmann, Horst (2010). Yeast. Molecular and Cell biology. Wiley-Blackwell. ISBN 352732609X.

Hader, C. P., Juan, R. A., José, Z. M. (2013). Simultaneous saccharification and fermentation of cassava stems. Dyna, 80 (180): 97-104. [9]A. O. A. C (2010). Official Methods of Analysis of Chemistry, (18th Ed.) Washington, D.C. Association of Official Analytical Chemists.

Hamelinck, C. N., Hooijdonk, V. G. and Faaij, A. P. C. (2005). Ethanol from lignocellulosic biomass: techno- economic performance in short-, middle- and long term. Biomass and Bioenergy,28: 384-410.

https://doi.org/10.1016/j.biombioe.2004.09.002

Harun, R. and Danquah, M. K. (2010). Influence of acid pre-treatment on microalgal biomass for bioethanol production. Process Biochemistry, 46 (1): 304-309.

https://doi.org/10.1016/j.procbio.2010.08.027

Lakkana, L., Pongthep, A., Pattana, L. and Prasit, J. (2012). Repeated-Batch Ethanol Production from Sweet Sorghum Juice by Saccharomyces cerevisiae Immobilized on Sweet Sorghum Stalks. Energies, 5: 1215-1228. Ojumu, T. V., Solomon, B. O., Betiku, E., Layoku, S. K. and Amigun, B. (2003).

https://doi.org/10.3390/en5041215

Cellulose production by Aspergillus flavusin isolate NSPR101 fermented in sawdust, corn corb and bagasse. African Journal of Biotechnology, 2(6):150-152.

https://doi.org/10.5897/AJB2003.000-1030

Omoniyi, T. E., Olorunnisola, A. O., (2014). Experimental Characterisation of Bagasse Biomass Material for Energy Production. International Journal of Engineering and Technology, 4(10).

Ratnasri, P. V., Lakshmi, B. K. M., Ambika, K. D. and Hemalatha, K. P. J. (2014). Isolation,characterization of Aspergillus fumigatus and optimization of cultural conditions for amylase production. International Journal of Research in Engineering and Technology, 3(02): 457-463.

https://doi.org/10.15623/ijret.2014.0302080

Rivera, E. C., Rabelo, S. C., Garcia, D. R., Macielfilho, R., Costa, A. C., (2010). Enzymatic hydrolysis of sugarcane bagasse for bioethanol production: Determining optimal enzyme loading using neural networks. Journal of Chemical Technology and Biotechnoloy, 85: 983-992.

https://doi.org/10.1002/jctb.2391

Saha, B. C., Iten, L. B., Cotta, M. A., Wu, Y. V. (2005). Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol. Process Biochemistry, 40, 3693-3700.

https://doi.org/10.1016/j.procbio.2005.04.006

Taherzadeh, M. J. and Niklasson, C. (2004). Ethanol from lignocellulosic materials: Pretreatment, acid and enzymatic hydrolysis, and fermentation. American Chemical Society Symposium Series, 889: 49-68.

https://doi.org/10.1021/bk-2004-0889.ch003

Wyman, C. E., (1996). Handbook on bioethanol: production and utilization. Taylor &Francis: Washington DC, USA.

Xiang, Q., Kim J., S. and Lee, Y. Y. (2003). A comprehensive kinetic model for dilute acid hydrolysis of cellulose. Applied Biochemistry and Biotechnology, 105: 108.

https://doi.org/10.1007/978-1-4612-0057-4_27

Zheng, Y., Pan, Z. and Zhang, R. (2009). Overview of biomass pretreatment for cellulosic ethanol production. International Journal of Biological Engineering, 2: 51-68.

Studies on Bioethanol Production from Rice Stalk using Co-cultures of Aspergillus niger and Saccharomyces cerevisiae

Authors

DOI:

Keywords:

Abstract

Downloads

References

Downloads

Published

How to Cite

Issue

Section

License

Most read articles by the same author(s)

Make a Submission