Antibiotic Susceptibility Patterns of Pseudomonas spp. Isolated from Soil Samples
DOI:
https://doi.org/10.47430/ujmr.1832.005Keywords:
Antibiotic, Mutation, Pseudomonas, Resistance and SusceptibilityAbstract
Antimicrobial resistance is one of the major problems facing the health sector. This has to do with many factors including changes in metabolic pathways and transfer of virulence genes among bacterial species among others. Therefore, this study aimed at testing the antibiotic susceptibility patterns of Pseudomonas spp. isolated from soil samples using the mastering-S (M13 and M14 disc). In this study, the susceptibility of 25 pseudomonas isolated from soil environment were tested using the mastering-S (13 and 14 disc). Overnight KB* cultures were used to inoculate KB* plates using the spread plate technique before placing the antibiotic disc on the surface of the agar and incubated at 20oC for 48hours. Antibiotic susceptibility was recorded based on the diameter of zone of inhibition. A diameter of 8mm and above was recorded as positive (susceptible) and a value of less than 8mm as negative (resistance). The results show that, all 25 isolates (100%) tested resistant to at least eight (8) antibiotics, while all the strains were susceptible to 10 µg Gentamicin (GM). Similarly, at least 8% were susceptible to 25 µg Chloramphenicol (C), 96% are susceptible to 10 µg Streptomycin (S) and about 92%, 40% and 48% of the isolates were found to be susceptible to 25 µg Tetracycline (T), 25 µg Colistin Sulphate (CO) and 200 µg Sulphatriad (ST) respectively. Therefore, the study concludes that different species of pseudomonad may respond to antibiotic differently and this should be considered especially when selecting drug of choice in medical settings.
Downloads
References
Amadi, E. S., Ugbogu, O, C. and Ogugbue, C. J. (2018). Principles of general microbiology. WEBSmedia communication limited edition, owerri Nigeria; First Edition.
Bossis, E., Lemanceau, P., Latour, X. and Gardan,L. (2000). The taxonomy of Pseudomonas fluorescens and Pseudomonas putida: current status and need for revision. Agronomie, 20(1), pp.51-63.
https://doi.org/10.1051/agro:2000112
CDC.(2013). The antibiotic resistance crisis. https://www.ncbi.nlm.nih.gov/pmc/artic les/PMC4378521/
EARS-Net .(2013). European Centre for Disease Prevention, and Control.Antimicrobial resistance surveillance in Europe 2012.Annual Report of the European Antimicrobial Resistance Surveillance Network (EARS-Net). Stockholm
Ghane, M. and Azimi, Z.(2014). Isolation, identification and antimicrobial susceptibility of Pseudomonas spp. isolated from hospital environment in Tonekabon, North of Iran. Journal of Applied & Environmental Microbiology, 2(4), pp.97-101.
Janda, J. M. and Abbott, S. L. (2002). Bacterial identification for publication: when is enough enough?. Journal of clinical microbiology, 40(6), pp.1887-1891.
https://doi.org/10.1128/JCM.40.6.1887-1891.2002
Kelch, W. J. and Lee, J. S. (1978).Antibiotic Resistance Patterns of Gram-Negative Bacteria Isolated from Envirorunmental Sources. Applied and Environmental Microbiology.36(3). 450-456
https://doi.org/10.1128/aem.36.3.450-456.1978
Li, J., Xie, S., Ahmed, S., Wang, F., Gu, Y., Zhang, C., Chai, X., Wu, Y., Cai, J. and Cheng, G. (2017). Antimicrobial activity and resistance: influencing factors. Frontiers in pharmacology, 8, p.364.
https://doi.org/10.3389/fphar.2017.00364
Line, L., Alhede, M., Kolpen, M., Kühl, M., Ciofu, O., Bjarnsholt, T., Moser, C., Toyofuku, M., Nomura, N., Høiby, N. and Jensen, P.O. (2014). Physiological levels of nitrate support anoxic growth by denitrification of Pseudomonas aeruginosa at growth rates reported in cystic fibrosis lungs and sputum. Frontiers in microbiology, 5, p.554.
https://doi.org/10.3389/fmicb.2014.00554
Livermore, D. M. (2002). Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: our worst nightmare?. Clinical infectious diseases, 34(5), pp.634-640.
https://doi.org/10.1086/338782
Luczkiewicz, A., Kotlarska, E., Artichowicz, W., Tarasewicz, K. and Fudala-Ksiazek, S. (2015). Antimicrobial resistance of Pseudomonas spp. isolated from wastewater and wastewater-impacted marine coastal zone. Environmental Science and Pollution Research, 22(24), pp.19823-19834.
https://doi.org/10.1007/s11356-015-5098-y
Moore, E. R., Mau, M., Arnscheidt, A., Böttger, E. C., Hutson, R. A., Collins, M. D., Van De Peer, Y., De Wachter, R. and Timmis, K.N. (1996). The Determination and Comparison of the 16S rRNA Gene Sequences of Species of the Genus Pseudomonas (sensu stricto and Estimation of the Natural Intrageneric Relationships. Systematic and Applied Microbiology, 19(4), pp.478-492.Van Delden, C. (2004). Virulence factors in Pseudomonas aeruginosa. In Virulence and Gene Regulation (pp. 3-45). Springer, Boston, MA.
https://doi.org/10.1016/S0723-2020(96)80021-X
Yamamoto, S., Kasai, H., Arnold, D. L., Jackson, R. W., Vivian, A. and Harayama, S. (2000). Phylogeny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes. Microbiology, 146(10), pp.2385- 2394.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 UMYU Journal of Microbiology Research (UJMR)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
