Antibacterial Activity and Phytochemical Screening of Stem Bark Extracts Of Adansonia Digitata on Some Clinical Isolates
DOI:
https://doi.org/10.47430/ujmr.1831.001Keywords:
Adansonia digitata, Extracts, Phytochemical screening, Antibacterial activity, Minimum Inhibitory Concentration, Minimum Bactericidal ConcentrationAbstract
Adansonia digitata stem bark extract is been used in the treatment of stomach upset, diarrhoea, dysentery, antioxidant, antimaleria, antiinflammation. This work was aimed at determine the phytochemical constituents and the antibacterial activities of petroleum ether, ethanol and aqueous crude extracts of the stem bark from Adansonia digitata on some clinical isolates. The Dried stem barks powdered of Adansonia digitata were extracted using three solvents. The crude extracts were subjected to Phytochemical screening using standard procedureThe crude extracts were further tested for their antibacterial activity using disc diffusion method against Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aerugi nosa, Klebsiella pneumonia, Escherichia coli and Proteusmirabilis. MICs were determined using micro broth dilution technique. Acute toxicity study was carried out using albino Wister rats. The ethanol extract as the highest percentage yields of 5.14%. The result of phytochemical screening shows the presence of tannins, saponins, alkaloids, flavonoids, triterpenoids, glycosides, steroids and reducing sugar. The results of antibacterial activity show that all the isolates showedsignificant susceptibility except Escherichia coli that was resistance to all the test concentratio n of the three extracts. The MIC and MBC of the different extract ranged from 500µg/ml to 2000 µg/ml and 1000µg/ml to 4000µg/ml respectively. The result of this study showed that the stem bark extract of Adansonia digitata contain bioactive constituents which are responsible for the antibacterial activity and may have the potential for the production of drugs against some clinical isolates.
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