Isolation and Identification of Fungi from Some Selected Vegetables in Kankara Local Government Area, Katsina State

Authors

  • Samaila Samaila Yaradua Centre for Biodiversity and Conservation, Department of Biology, Umaru Musa Yaradua University, P.M.B 2218, Katsina, Nigeria
  • Shamsuddeen Lawal Kankara Centre for Biodiversity and Conservation, Department of Biology, Umaru Musa Yaradua University, P.M.B 2218, Katsina, Nigeria
  • Murtala Yusuf Department of Biology, Al-Qalam University, Katsina, Nigeria

DOI:

https://doi.org/10.47430/ujmr.1831.012

Keywords:

Fungal species, Identification, Isolation, Kankara, Vegetables

Abstract

Samples of vegetables (infected and healthy) were investigated in this study to identify and isolate fungal species growing present. The vegetables are Tomato (Lycopersicum esculentum), Pepper (Capsicum annum), Cucumber (Cucumis sativus) and Onion (Allium cepa) which were collected from local farms around Kankara local government area. Potato dextrose agar (PDA) was used for isolation of the fungi and morphological features and fungal identification key were used to identify the isolated organisms. All the vegetables collected showed the presence of fungi. The fungi isolated are Aspergillus flavus, Rhizopus stolonifer, Aspergillus niger, Aspergillus parasiticus, Penicillium digitatum., Penicillium citrinum and Mucor spp. among all, Penicillium citrinum, Aspergillus niger., and Aspergillus. parasiticus had the highest rate of occurrence followed by Penicillium digitatum and Aspergillus flavus while Mucor spp. and Rhizopus stolonifer were the least.

Downloads

Download data is not yet available.

References

Adebayo-Tayo, B.C., Odu, N.N., Esen, C.U. and Okonko I.O. (2012). Microorganisms Associated With Spoilage Of Stored Vegetables In Uyo Metropolis, AkwaIbom State, Nigeria. Nature and Science 10(3):23-32

Al-Hindi, R.R., Al-Najada, A.R., Mohamed, S.A. (2011). Isolation and identification of some fruit spoilage fungi: Screening of plant cell wall degrading enzymes. African Journal ofMicrobiology Research, 5(4): 443-448.

Asagbra, A. and Oyewole O.B. (2002). Fermentation studies on carrot juice processed to table wine. Nig Foo 30,74-7.

Baiyewu et al., (2007). This is in correlation with the work of Nijis et al., (1997) who reported that Aspergillus spp. is the predominant organism associated with the spoilage of orange. The isolation of Aspergillus flavus, Penicilliuum citrinum and Aspergillus parasiticus from Tomato and Cucumber (healthy and infected) is also congruent with findings of Efiuvwevwere, (2000) who reported that Aspergillus spp. are responsible for the rotting of pineapple. The isolation of Rhizopus spp. and Mucor spp. from Tomato and Cucumber (infected) agreed with work of Efiuvwevwere, (2000); Nijis et al., (1997); Purseglove, (1977), who reported that Fusarium spp. and Rhizopus spp. are responsible for the soft rot of tomato.

Baiyewu, R.A., Amusa, N.A., Ayoola, O.A. and Babalola, O.O. (2007). Survey of the postharvest diseases and aflatoxin contamination of marketed Pawpaw fruit (Carica papaya L.) in South Western Nigeria

Barth, M., Hankinson, T.R., Zhuang, H. and Breidt, F. (2009). Microbiological Spoilage of Fruits and Vegetables.

https://doi.org/10.1007/978-1-4419-0826-1_6

W.H. Sperber, M.P. Doyle (eds.), Compendium of the Microbiological Spoilage of Foods and Beverages, Food Microbiology and Food Safety. Springer Science Business Media, LLC, Pp135-183.

Burnett, S. and Beuchat, L. (2001) Foodborne pathogens: human pathogens associated with raw produce and unpasteurized juices, and difficulties in decontamination. Journal of Industrial Microbiology & Biotechnology. 27, 104-10.

https://doi.org/10.1038/sj.jim.7000199

Chukwuka, K.S., Okonko, I.O. and Adekunle A.A. (2010). Microbial Ecology of Organisms Causing Pawpaw (Carica Papaya L.) Fruit Decay in Oyo State, Nigeria. American- Eurasian Journal of Toxicological Sciences, 2(1): 43-50

Domsch, K.H., Gams, W. and Anderson, T.H. (1993) Compedium of Soil Fungi. Academic Press. London, p. 860.

Eaton, D.L., Groopman, J.D., editors (1994). The Toxicology of Aflatoxins: Human Health, Veterinary, and Agricultural Significance. San Diego, CA: Academic Press.

https://doi.org/10.1016/B978-0-12-228255-3.50005-2

https://doi.org/10.1016/B978-0-12-228255-3.50004-0

https://doi.org/10.1016/B978-0-12-228255-3.50008-8

Efiuvwevwere, B.J.O. (2000). Microbial spoilage agents of tomato and assorted fruits and vegetable (An illustrated references book). Paragraphics of lishing company, Port Harcourt, Pp: 1-39.

Hung, H.C., Joshipura, K.J.,Jiang, R., Hu, F.B.,Hunter, D.,Smith-Warner

S.A.,Colditz, G.A., Rosner, B.,Spiegelman, D., and Willett W.C. (2004).Fruit and vegetable intake and risk of major chronic disease. 96(21):1577-84

https://doi.org/10.1093/jnci/djh296

ICMSF (International Commission on Microbiological Specification for Foods) (1998).Microorganisms in foods, Microbial Ecology of food commodities. New York, Blackie Academics and Professional 6: 617.

James, M. J., Martin J. L., and David A. G. (2005) Modern Food Microbiology Modern FoodMicrobiology.7thNewYork,NYAvailabl eat:http://works.bepress.com/david_gol den/ 13/

Klich, M.A. (2002) Identification of Common Aspergillus Species. Centraal bureau voor Schim, Utrecht.

Krogh, P., 1992. Adverse Effect of Mycotoxins on Human Health. In: Seed Pathology, Mathur, S.B. and J. Jorgensen (Eds.). Copenhagen, Denmark, pp: 149-157.

Merz, W. G., Roberts G. D. (1995). Detection and recovery of fungi from clinical specimens. In: Murray P R, Baron E J, Pfaller M A, Tenover F C, Yolken R H, editors. Manual of clinical microbiology. Washington, D.C: American Society for Microbiology;. pp. 709-722.

Monso, E.M. (2004). Occupational asthma in greenhouse workers, Curr. Opin. Pulm. Med., 10: 147-150. 30.

https://doi.org/10.1097/00063198-200403000-00010

Muhammad, S., Shehu, K. and Amusa, N.A. (2004). Survey of the market diseases and aflatoxin contamination of Tomato (Lycopersicon esculentum MILL) fruits in Sokoto North western Nigeria. Nutrition and Food Sci. (UK) 34:72-76.

https://doi.org/10.1108/00346650410529032

Nguyen-the, C. and Carlin, F. (1994). The microbiology of minimally processed fresh fruits and vegetables. Critical Reviews in Food Science & Nutrition 34(4): 371-401.

https://doi.org/10.1080/10408399409527668

Nijis, D. V., Egmerd, H.P. Rombouts, F.M. and Notermans, S.H.W. (1997). Identification of hazardous Fusarium secondary metabolites occurring in Food Raw Materials. Journal of food safety;17: 16-191

https://doi.org/10.1111/j.1745-4565.1997.tb00185.x

Peraica, M., Radic, B., Lucic, A. and Pavlovic M. (1999). Toxic effects of mycotoxins in humans Bull. World Health Org., 77, pp.754-763

Petzinger, E. and Weidenbach, A. (2002) Mycotoxins in the food chain: The role of ochratoxins. Livestock ProductionScience 76(3):245-250

https://doi.org/10.1016/S0301-6226(02)00124-0

Pitt J.I., Hocking A.D., (1997). Fungi and food spoliage. 2ndEd. Blackie Academic andProfessional, London, UK.

https://doi.org/10.1007/978-1-4615-6391-4

Prasad, T., (1992). Detection of Fungi in Stored Grains and Estimation of Mycotoxins. In: Seed Pathology, Mathur, S.B. and J. Jorgensen (Eds.). Technical Centre for Agricultural and Rural Co-operation, Denmark, pp: 175-181

Purseglove, W. (1977). Tropical Crops Dicotyledons vol. 1 and 2 ELBS, pp: 137-533.

Robert, A. S. and Janos V. (2007). Aspergillus systematics in the genomic era - Preface. Studies in Mycology 59(59)

https://doi.org/10.1016/S0166-0616(14)60167-6

Tournas, V.H. and Stack, M.E. (2001). Production of alternariol and alternariol methyl ether by Alternaria alternatagrown on fruits at various temperatures, Journal of Food Protection, 64: 528-532.

https://doi.org/10.4315/0362-028X-64.4.528

Yusuf, I.Z., Oyaweye, O.M., Yongab, K.A. and Pemu A.T. (2004). Bacteriological Quality Assessment of Salad Vegetables sold in Bauchi Metropolis. Nigeria Journal ofMicrobiology 18:316-320.

Downloads

Published

30-06-2018

How to Cite

Samaila Samaila Yaradua, Shamsuddeen Lawal Kankara, & Murtala Yusuf. (2018). Isolation and Identification of Fungi from Some Selected Vegetables in Kankara Local Government Area, Katsina State. UMYU Journal of Microbiology Research (UJMR), 3(1), 76–80. https://doi.org/10.47430/ujmr.1831.012