Assessment of the Microbial Quality of Food Sold in Some Restaurants within Katsina Metropolis
DOI:
https://doi.org/10.47430/ujmr.2382.007Keywords:
Antibiotic, Assay, Satisfactory, Specification, ICMSFAbstract
Foods are substances that when consumed provide the body with nutrients for growth and development. Foods for consumption should be within the acceptable limit of microbial load, exceeding it may lead to food-borne intoxication and illness. This study aimed to determine the microbial quality of commonly sold foods in some restaurants within Katsina metropolis. Physiochemical properties of the food namely; pH, temperature, colour, and taste were determined using a pH meter, thermometer, and organoleptic approach respectively. Conventional methods of identification were used to identify the isolates and a modified method by Kirby Bauer was employed to determine antimicrobial assays of the identified isolates. The results obtained showed that the physiochemical parameters of pH and temperature range between 5.50 to 7.40, and 30.0 oC to 32.0 oC respectively with a normal taste and colour. Total bacterial (1.04 x107 CFU/g) and fungal (4.8 x106 CFU/g) counts of the samples fall within the unsatisfactory limits set by the International Commission on Microbiological Specifications for Food (ICMSF). A total of nine (9) bacteria and five (5) fungi were identified from fifteen (15) samples examined. E. coli (26.31%) was most prevalent followed by Enterobacter aerogens and Staphylococcus epidermis (15.78%), Bacillus subtilis, and Salmonella spp (10.52%) while Proteus mirabilis, Micrococcus luteus, Pseudomonas aeroginosa, and Clostridium spp. have the least occurrence (5.26%). Aspergillus flavus (45.45%) was most prevalent among the fungal isolates followed by Mucor (27.27%) and Botrytis, Alternaria, and Fusarium chlamydosporum (9.09%) have the least occurrence. The antimicrobial assay shows that Gentamycin, levofloxacin, Erythromycin, and Ciprofloxacin were effective against Micrococcus luteus, Staphylococcus spp., Clostridium spp., and Bacillus subtilis. Norfloxacin and Amoxicillin were resisted by Micrococcus luteus and Staphylococcus spp., Ketoconazole and Fluconazole inhibited Mucor and Aspergillus flavus but were resisted by Alternaria, Botrytis, and Fusarium chlamydosporum. It was concluded that foods sold in some restaurants within the Katsina metropolis fall within the unsatisfactory limits of ICMSF standards, thus, improved strict hygiene compliance is recommended.
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Akpoka, A.O., Okwu, M.U., Imade, O.S., Enaigbe, A.A., Solanke, E. O., Erifeta, G.O. and Izevbuwa, E. O. (2019). Microbial assessment of ready-to-eat food and food contact surfaces in selected restaurants in Okada, south-south Nigeria, Bacterial empire. 2(3), 58-63. https://doi.org/10.36547/be.2019.2.3.58-63
Alex Logan., Alexandar Abigail and Aiden Samuel. (2017). The causes and Effects of foodborne illness (Food Poisoning). International Network Organization for Scientific Research (http://www.inosr.net/inosr-applied-sciences/ INOSR APPLIED SCIENCES3 (1): 9-15, 20179
Bintsis Thomas. (2017). a review foodborne pathogen, AIMS Microbiology Volume 3, Issue 3, 529-563. https://doi.org/10.3934/microbiol.2017.3.529
Canadian institute of food safet.(2022).https://www.foodsafety.ca/blog/food-safety-and-types-food-contamination
Croxen, M.A, Law, R. J, Scholz, R. (2013). Recent advances in understanding enteric pathogenic Escherichia coli. Clinical Microbiology. 26: 822-880. https://doi.org/10.1128/CMR.00022-13
David, R. J., Jaako, M. and Katie., A. M. (2012). The importance of food. International Journal of Advances in Scientific Research and Engineering;166(2):187-188. DOI:10.31695/ 2020.33736.
Fowoyo Patience Temitope, Ridwan Baba-Ali.(2015), Microbiological Assessment of Fast Foods Sold in Lokoja Metropolis, Nigeria. Open Access Library Journal, 2:e1541. http://dx.doi.org/10.4236 /oalib.1101541
Garcia, A., Fox, J.G. and Besser, T.E. (2010). Zoonotic enterohemorrhagic Escherichia coli: A one health perspective. 51: 221-232. https://doi.org/10.1093/ilar.51.3.221
Johnston, L.M., Moe, C.L., Moll, D., & Jaykus, L. (2006). The epidemiology of produce-associated outbreaks of foodborne disease.In: Microbial Hazard Identification in Fresh Fruits and Vegetables. New Jersey: john wiley and sons. https://doi.org/10.1002/0470007761.ch2
Lina A. Omar-Zahid. (2013). Atlas of food microbiology laboratory.1st electronic edition. pp. 22-24 and 26.
Lutpiatina, L. (2021). Pathogens Transmitted through Contaminated Rice. Recent Advances in Rice Research. https://doi.org/10.5772/intechopen.93757
Magar Sanjogta thapa. (2022). Factors affecting the growth of microorganisms in food. Accessed from https://microbenotes.com/factors-affecting-the-growth-of-microorganisms-in-food/ (10/08/2023)
Moral, U., Nagar, P., and Kaur, K. (2017). Growth of Different Types of Microorganism, Intrinsic and Extrinsic Factors of Microorganism and their Affects in Food: A Review International Journal Current Microbiological Application Science. 6(1), 290-298. https://doi.org/10.20546/ijcmas.2017.601.035
Murray, P.R., Rosenthal, K.S., and Faller, M.A.(2016). Medical Microbiology 8th edition. Elsevier International Canada
Nester, E. W., Andrew, D. G., Roberts, C. E., Pearshall, N.M. and Nester, M. T. (2004). Microbiology: A Human Perspective (fouth edition). McGraw Hill Companies Inc., New York, Pp. 802 - 803.
Nyenje, M.E., Odjadjare, C.E., Tanih, N.F., Green, E. and Ndip, R.N. (2012). Foodborne Pathogens Recovered from Ready-to-eat foods from roadside cafeterias and retail outlets in Alice, Eastern Cape Province, South Africa: Public Health Implications. International Journal Environmental Research Public Health, 9(8):2608-19. https://doi.org/10.3390/ijerph9082608
Oranusi, S and Okechukwu, I.O. (2013). Microbiological quality assessment of foods sold in students cafeterias. Global Research Journal of Microbiology, 3(1).1-7. http://www.globalresearchjournals.org/?a=journal&id=grjm
Rolfe, C., Daryaei, H. (2020). Intrinsic and Extrinsic Factors Affecting Microbial Growth in Food Systems. In: Demirci, A., Feng, H., Krishnamurthy, K. (eds) Food Safety Engineering. Food Engineering Series. Springer, Cham. https://doi.org/10.1007/978-3-030-42660-6_1
Sabuj, A.A.M., Haque Z.F., Younus, M.I., Pondit, A., Barua, N., Hossain, M.G., Islam, M.A. and Saha, S. (2020). Microbial risk assessment of ready-to-eat fast foods from different street vended restaurants, International Journal One Health, 6 (1):41-48. https://doi.org/10.14202/IJOH.2020.41-48
Varghese Naveena and Joy P.P. (2014). Pineapple Research Station (Kerala Agricultural University), Vazhakulam-686670, Muvattupuzha, Ernakulam, Kerala. https://www.researchgate.net/publication/306018042_Microbiology_Laboratory_Manual
Varghese Naveena, Joy P.P., and Divya B. (2015), Microbiological and chemical testing of parameters of foods at: https://www.researchgate.net/publication/306037462
Wogu, M. D., Omoruyi, M. I., Odeh, H. O. and Guobadia, J. N. (2010). Microbial load in ready-to-eat rice sold in Benin. City Journal of Microbiology and Antimicrobials, 3(2). 29-33, ISSN 2141-2308 Available online http://www.academicjournals.org/JMA
World Health Organization. (2021, November 17). Antimicrobial resistance. Retrieved from: https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance
World Health Organization. (2022). https://www.who.int/news-room/fact-sheets/detail/foodsafety#:~:text=An%20estimated%20600%20million%20%E2%80%93%20almost,healthy%20life%20years%20(DALYs).
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