Phenotypic Identification and Antibiotics Susceptibility Profile of Staphylococcus aureus from Surgical Equipment and Hospital Environment in Lokoja, Kogi State, Nigeria
DOI:
https://doi.org/10.47430/ujmr.2272.002Keywords:
Staphylococcus aureus, antimicrobial susceptibility profile, surgical equipment, Hospital environment, LokojaAbstract
Staphylococcus aureus is one of the prominent causes of hospital-acquired bacteremia. Despite the availability of anti-staphylococcal antibiotics, hospital acquired S. aureus bacteremia is still a major problem with considerable morbidity and mortality. Therefore, the aim of this study was to isolate, identify and determine the Antibiotics susceptibility profile of Staphylococcus aureus from the surfaces of surgical equipment and environment of major public and private hospitals in Lokoja, Kogi State, Nigeria using colonial characteristics, microscopy and conventional biochemical techniques. The Antibiotics susceptibility profile of the isolates was determined in accordance with the Guidelines of Clinical and Laboratory Standard Institute (CLSI). A total of three hundred and fifty (350) swab samples comprising of fourty (40) from surgical equipment and three hundred and ten (310) from the environment were collected from three (3) different public and private hospitals within Lokoja metropolis. The results obtained showed that 110(31.4%) of samples from the hospital environment were confirmed positive for Staphylococcus aureus with Hospital A constituting 30(8.6%), Hospital B had 59(16.8%) and Hospital C recorded 21 (6.0%). Of the 19 selected S. aureus isolates for antimicrobial susceptibility screening, 10.52% and 5.26% were intermediately resistant to Norfloxacin and Chloramphenicol respectively. Furthermore, the screened S. aureus isolates showed 100% susceptible to Ciprofloxacin, Gentamicin and Erythromycin; 94.73% susceptible to Chloramphenicol and 89.47% susceptible to Levoflaxin. The result also revealed 100% resistance to Penicillin and 15.78% resistance to Rifampicin. The high presence of Staphylococcus aureus in the hospital environment is a potential threat to the health of the patients and the public as this organism has been implicated in several human diseases, especially hospital- acquired bacteremia. Therefore, improved personal and public hygienic practices within the hospitals are required to reduce the high presence of S. aureus and other pathogenic microorganisms.
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Al-Zoubi, M. S., Al-Tayyar, I. A., Hussein, E., Al Jabali, A., & Khudairat, S. (2015). Antimicrobial susceptibility pattern of Staphylococcus aureus isolated from clinical specimens in Northern area of Jordan. Iranian journal of microbiology, 7(5), 265.
Aminu, M., Usman-Sani, H., and Usman, M. A. (2014). Characterization and determination of antibiotic susceptibility pattern of bacteria isolated from some fomites in a teaching hospital in northern Nigeria. African Journal of Microbiology Research, 8(8), 814-818. https://doi.org/10.5897/AJMR2013.6512
Asbell, P. A., Sahm, D. F., Shaw, M., Draghi, D. C., and Brown, N. P. (2008). Increasing prevalence of methicillin resistance in serious ocular infections caused by Staphylococcus aureus in the United States: 2000 to 2005. Journal of Cataract and Refractive Surgery, 34(5), 814-818. https://doi.org/10.1016/j.jcrs.2008.01.016
Buba, F. N., Ojinnaka, O. C., Ndukwu, R. I., Agbaje, G. I., &Orofin, Z. O. (2021). Assessment of flood vulnerability in some communities in Lokoja, Kogi State, Nigeria, using participatory geographic information systems. International journal of disaster risk reduction, 55, 102111. https://doi.org/10.1016/j.ijdrr.2021.102111
Calfee, D. P., Salgado, C. D., Milstone, A. M., Harris, A. D., Kuhar, D. T., Moody, J., ... & Yokoe, D. S. (2014). Strategies to prevent methicillin-resistant Staphylococcus aureus transmission and infection in acute care hospitals: 2014 update. Infection Control & Hospital Epidemiology, 35(7), 772-796. https://doi.org/10.1086/676534
Carey, A. J., Saiman, L., & Polin, R. A. (2008). Hospital-acquired infections in the NICU: epidemiology for the new millennium. Clinics in perinatology, 35(1), 223-249. https://doi.org/10.1016/j.clp.2007.11.014
Chinedum, I. E. (2005). Microbial resistance to antibiotics. African journal of Biotechnology, 4(13),1606-1611 https://doi.org/10.4314/ajfand.v4i13.71776
Clinical and Laboratory Standard Institute (CLSI) (2016). Performance Standards for Antimicrobial Susceptibility Testing. 26th edition. CLSI Supplement M100S. Wayne PA, USA.
Filius, P. M., and Gyssens, I. C. (2002). Impact of increasing antimicrobial resistance on wound management. American journal of clinical dermatology, 3(1), 1-7. https://doi.org/10.2165/00128071-200203010-00001
Ghias, W., Sharif, M., Yazdani, F. A., & Rabbani, M. (2016). Isolation and identification of Methicillin and Vancomycin resistance Staphylococcus aureus from pus samples of injured skin patients in Lahore, Pakistan. Biomedical Letters, 2(2), 103-112.
Gibb, B. P., & Hadjiargyrou, M. (2021). Bacteriophage therapy for bone and joint infections: an instructional review. The bone & joint journal, 103(2), 234-244. https://doi.org/10.1302/0301-620X.103B2.BJJ-2020-0452.R2
Holmes, A., Ganner, M., McGuane, S., Pitt, T. L., Cookson, B. D., and Kearns, A. M. (2005). Staphylococcus aureus isolates carrying Panton-Valentine leucocidin genes in England and Wales: frequency, characterization, and association with clinical disease. Journal of clinical microbiology, 43(5), 2384-2390. https://doi.org/10.1128/JCM.43.5.2384-2390.2005
Lee, A. S., De Lencastre, H., Garau, J., Kluytmans, J., Malhotra-Kumar, S., Peschel, A., & Harbarth, S. (2018). Methicillin-resistant Staphylococcus aureus. Nature reviews Disease primers, 4(1), 1-23. https://doi.org/10.1038/nrdp.2018.33
Omololu, J., & Bamidele, K. F. (2017). Antimicrobial Susceptibility Pattern of S. aureus and Salmonella sp isolated from Poultry Feed Sold in Ile Ife, Nigeria. Archives of Clinical Microbiology, 8(3), 0-0.
Omololu-Aso, J., Kolawole, D. O., Omololu-Aso, O. O., Dan Ajisebutu, S. O. (2011). Antibiotics sensitivity pattern of Staphylococcus aureus from fomites in the Obafemi Awolowo University Teaching Hospital Complex (OAUTHC) Nigeria. International Journal of Medical Sciences, 3, 32-6.
Onwubiko, N. E., and Sadiq, N. M. (2011). Antibiotic sensitivity pattern of Staphylococcus aureus from clinical isolates in a tertiary health institution in Kano, Northwestern Nigeria. Pan African Medical Journal, 8(1). https://doi.org/10.4314/pamj.v8i1.71050
Pal, S., Sayana, A., Joshi, A., & Juyal, D. (2019). Staphylococcus aureus: A predominant cause of surgical site infections in a rural healthcare setup of Uttarakh and. Journal of Family Medicine and Primary Care, 8(11), 3600. https://doi.org/10.4103/jfmpc.jfmpc_521_19
Rantala, S. (2014). Streptococcus dysgalactiae subsp. equisimilis bacteremia: an emerging infection. European journal of clinical microbiology & infectious diseases, 33(8), 1303-1310. https://doi.org/10.1007/s10096-014-2092-0
Sadeghi, J., & Mansouri, S. (2014). Molecular characterization and antibiotic resistance of clinical isolates of methicillin‐resistant Staphylococcus aureus obtained from Southeast of Iran (Kerman). Apmis, 122(5), 405-411. https://doi.org/10.1111/apm.12158
Sarmukaddam, S.B and Gerald, S.G. (2004). Validity of assumption while determining sample size. Indian Journal of Community Medicine, 2:87-91.
Suleiman, A.B., Makolo, D., Ahmad, A.E., Tahir, M.I., and Dikwa, K.B. (2019). Phenotypic Characterization of Staphylococcus aureus isolated from cases of bovine mastitis in parts of Plateau State, Nigeria. Nigerian Journal of Microbiology, 33(1):4587-4596.
Wertheim, H. F., Melles, D. C., Vos, M. C., van Leeuwen, W., van Belkum, A., Verbrugh, H. A., and Nouwen, J. L. (2005). The role of nasal carriage in Staphylococcus aureus infections. The Lancet infectious diseases, 5(12), 751-762. https://doi.org/10.1016/S1473-3099(05)70295-4
Xiong, W., Sun, Y., Ding, X., Wang, M., and Zeng, Z. (2015). Selective pressure of antibiotics on ARGs and bacterial communities in manure-polluted freshwater-sediment microcosms. Frontiers in Microbiology, 6, 194. https://doi.org/10.3389/fmicb.2015.00194
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