Assessing the Antibacterial Properties of Aloe vera (Linn) and Aloe schweinfurthii (Baker) against some Plant Pathogenic Bacteria
DOI:
https://doi.org/10.47430/ujmr.2491.020Keywords:
Aloe vera, Aloe schweinfurthii, Leaf extract, Antimicrobial activities, Bacterial pathogensAbstract
Study’s Novelty/Excerpt
- This study assesses antibacterial efficacy of Aloe vera and Aloe schweinfurthii leaf extracts against plant pathogenic bacteria, highlighting significant differences in effectiveness between aqueous and ethanolic extracts.
- The findings reveal that A. schweinfurthii extracts, particularly the ethanolic form, demonstrate superior antibacterial activity compared to A. vera, with inhibition zones up to 14.06mm.
- Additionally, the identification of diverse phytochemicals such as alkaloids, tannins, and flavonoids in the extracts underscores their potential as novel antibacterial agents for managing plant pathogenic bacteria.
Full Abstract
The study aimed to assess the effectiveness of Aloe vera and Aloe schweinfurthii leaf extracts against plant pathogenic bacteria. Aqueous (cold and hot) and ethanolic extracts from both plants were tested on Xanthomonas axonopodis, Pseudomonas syringae, and Pseudomonas aeruginosa. Results showed antibacterial properties in both extracts, with varying inhibition zones ranging from 0.01 to 14.06mm. The most effective was the ethanolic extract from powdered A. schweinfurthii at 0.05g/ml, while hot water extracts from both species were the least effective. Phytochemical screening revealed alkaloids, tannins, saponins, flavonoids, cardiac glycosides, phytates, and oxalates in both extracts. The study concluded that A. schweinfurthii outperformed A. vera in managing the plant pathogenic bacteria, suggesting potential new antibacterial agents.
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References
Abd-Alla, M. H., Bashandy, S. R., Schnell, S. and Ratering, S. (2011). Isolation and characterization of Serratia rubidaea from dark brown spots of tomato fruits. Phytoparasitica, 39, 175-183. https://doi.org/10.1007/s12600-011-0148-6.
Adamu, H.M., Abayeh, O.J. and Agho, M.O. (2007). Phytochemical screening and antimicrobial activity of the extracts of Delarium microcarpum and Ziziphus mucronata. Nigerian Journal of Botany. 20(2): 327-334.
Alejo, A.O., Ajayi, A.M. and Akinyele, B. O. (2019). Comparative Efficacy of Aloe vera (Linn) and Aloe schweinfurthii (Baker) Powdered Leaf Extracts in the Control of Some Plant Fungal Pathogens. International Journal of Biochemistry Research & Review. 25(2): 1-9, 2019; Article no.IJBCRR.47190. https://doi.org/10.9734/ijbcrr/2019/v25i230072
Arbab, S., Ullah, H., Weiwei, W., Wei, X., Ahmad, S. U., Wu, L. and Zhang, J. (2021). Comparative study of antimicrobial action of Aloe vera and antibiotics against different bacterial isolates from skin infection. Veterinary Medicine and Science, 7(5), 2061-2067. https://doi.org/10.1002/vms3.488
Block, A., Li, G., Fu, Z. Q., and Alfano, J. R. (2008). Phytopathogen type III effector weaponry and their plant targets. Current opinion in plant biology, 11(4), 396-403. https://doi.org/10.1016/j.pbi.2008.06.007
Cushmie, T.P. and Lamb, A.J. (2005). Antimicrobial activities of flavonoids. International Journal of Antimicrobial Agents, 26 (5): 343-356. https://doi.org/10.1016/j.ijantimicag.2005.09.002
Danish, P., Ali, Q., Hafeez, M. M., and Malik, A. (2020). Antifungal and Antibacterial Activity of Aloe vera plant extract. Biological and Clinical Sciences Research Journal, 2020(1). https://doi.org/10.54112/bcsrj.v2020i1.4
Eno, I.P. (2011). Field trial on the potential of Anacardium occidentalis for the control of cowpea anthracnose disease in Nigeria. A MSc Thesis Presented to the Department of Plant Health Management, Michael Okpara University of Agriculture, Umudike.
Enyiukwu, D. N., Awurum, A. N. and Nwaneri, J. A. (2014). Efficacy of plant-derived pesticides in the control of myco-induced postharvest rots of tubers and agricultural products: A review. Net Journal of Agricultural Science, 2(1), 30-46. https://doi.org/10.15580/GJMA.2014.3.0521014241
Gadd, G. M. (2007). Geomycology: biogeochemical transformations of rocks, minerals, metals and radionuclides by fungi, bioweathering and bioremediation. Mycological research, 111(1), 3-49. https://doi.org/10.1016/j.mycres.2006.12.001
Gao, J., Wang, Y., Wang, C. W. and Lu, B. H. (2014). First report of bacterial root rot of ginseng caused by Pseudomonas aeruginosa in China. Plant Disease, 98(11), 1577-1577. https://doi.org/10.1094/PDIS-03-14-0276-PDN.
George, F., Kareem, Z., Harrinder, P., Makkar, S. and Becker, K. (2002). ʻʻThe biological action of saponins in animal system.’’ British Journal of Nutrition. 88(6): 587-605.Germplasm Resources Information Network, United States Department of Agriculture. https://doi.org/10.1079/BJN2002725
Goodell, B., Jellison, J., Liu, J., Daniel, G., Paszczynski, A., Fekete, F. and Xu, G. (1997). Low molecular weight chelators and phenolic compounds isolated from wood decay fungi and their role in the fungal biodegradation of wood. Journal of Biotechnology, 53(2-3), 133-162. https://doi.org/10.1016/S0168-1656(97)01681-7
Grąz, M. (2024). Role of oxalic acid in fungal and bacterial metabolism and its biotechnological potential. World Journal of Microbiology and Biotechnology, 40(6), 178. https://doi.org/10.1007/s11274-024-03973-5
Gurjar, M.S., Ali, S., Akhtar, M. and Singh, K.S. (2012). Efficacy of plant extracts in plant disease management. Agricultural Sciences, 3(3):425. https://doi.org/10.4236/as.2012.33050
Herraiz, T. and Galisteo, J. (2004). Endogenous and dietary indoles: a class of antioxidants and radical scavengers in the ABTS assay. Free Radical Res 38:323-331. https://doi.org/10.1080/10611860310001648167
Holstege C.P. and Kirk M.A. (2022). Cyanide and Hydrogen Sulfide. In: Nelson LS, Howland M, Lewin NA, Smith SW, Goldfrank LR, Hoffman RS. eds. Goldfrank’s Toxicologic Emergencies, 11e. McGraw Hill; 2019. Accessed November 01, 2022. https://accessemergencymedicine.mhmedical.com/book.aspx?bookid=2569
Igbinosa, O.O., Igbinosa, E.O. and Aiyegoro, O.A. (2009). Antimicrobial activity and phytochemical screening of stem bark extracts of Jatropha curcas (Linn). Afr. J. Pharm. Pharmacol. 3(2): 58-62.
Janusz, G., Pawlik, A., Sulej, J., Świderska-Burek, U., Jarosz-Wilkołazka, A. and Paszczyński, A. (2017). Lignin degradation: microorganisms, enzymes involved, genomes analysis and evolution. FEMS microbiology reviews, 41(6), 941-962. https://doi.org/10.1093/femsre/fux049
Johns Hopkins University, Centre for Health Security, 2022. Factsheet Cyanide. Updated November 2, 2022. Factsheet | Cyanide (centerforhealthsecurity.org)
Jothi, J. S., Karmoker, P. and Sarower, K. (2014). Quality assessment of mized fruit squash: physico-chemical analysis, senory evaluation and storage studies. Journal of the Bangladesh Agricultural University, 12(452-2016-35642), 195-201. ISSN 1810-3030. https://doi.org/10.3329/jbau.v12i1.21412
Kana, H.A., Aliyu, I.A. and Chammang, H.B. (2012). Review on neglected and underutilized root and tuber crops as food security in achieving the millennium development goals in Nigeria. J Agric Vet Sci, 4:27-33.
Kumar, S., Jakhar, D. S., and Singh, R. (2017). Evaluating Antimicrobial Activity of Aloe vera Plant Extract in Human life. Biomed. J. Sci. Tech. Res, 1, 1854-1856. https://doi.org/10.26717/BJSTR.2017.01.000565
Kwak, A. M., Lee, I. K., Lee, S. Y., Yun, B. S. and Kang, H. W. (2016). Oxalic acid from Lentinula edodes culture filtrate: antimicrobial activity on phytopathogenic bacteria and qualitative and quantitative analyses. Mycobiology, 44(4), 338-342. https://doi.org/10.5941/MYCO.2016.44.4.338
Liu, R.H. (2000). Supplement quick fix fails to deliver. Food Technol Int 1:71-72.
Nascimento, G. G., Locatelli, J., Freitas, P. C. and Silva, G. L. (2000). Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology, 31(4): 247-256. https://doi.org/10.1590/S1517-83822000000400003
Odeleye, O.M. (2004). “Comparative chemical and biological analyses of Aloe schweinfurthiiand Aloe vera for laxative activity. M.Sc. Thesis, Obafemi Awolowo University, Nigeria.
Ogbuewu, I.P. (2008). Physiological responses of rabbits fed graded levels of neem (Azadirachta indica) leaf meal. M.Sc. Thesis, Federal University of Technology, Owerri.
Oshomoh, E., Idu, M., and Emeka-Katandu, I. E. (2014). Phytochemical Constituents and Antimicrobial Investigation of the Aqueous Extracts of the Gel and Leaf of Aloe vera (Aloe barbadensis MILLER) on selected Microorganisms. Nigerian Journal of Life Sciences (ISSN: 2276-7029), 4(2), 181-191. https://doi.org/10.52417/njls.v4i2.198
Salami, O. A. and Popoola, O. O. (2007). Thermal control of some post-harvest rot pathogens of Irish potato (Solanum tuberosum L.). Journal of Agricultural Sciences (Belgrade), 52(1): 17- 31. https://doi.org/10.2298/JAS0701017S
Salawu, K. M., Ajaiyeoba, E. O., Ogbole, O. O., Adeniji, J. A., Faleye, T. C., and Agunu, A. (2017). Antioxidant, Brine Shrimp Lethality, and Antiproliferative Properties of Gel and Leaf Extracts of Aloe schweinfurthii and Aloe vera. Journal of herbs, spices & medicinal plants, 23(4), 263-271. https://doi.org/10.1080/10496475.2017.1318328
Salawu, K. M., Oyerinde, A. A., Aliyu, A. and Obajemihi, O. I. (2020). Growth inhibitory properties and antimicrobial evaluation of Aloe schweinfurthii (Baker) leaf rind extract. Journal of Pharmacy & Bioresources, 17(2), 174-179. https://doi.org/10.4314/jpb.v17i2.11
Salisu, B. D., and Shema, M. (2019). Phytochemical Screening and Antimicrobial Activity of Aqueous Stem Extract of Aloe vera on Some Common Pathogenic Bacteria. UMYU Journal of Microbiology Research (UJMR), 4(2), 49-56. https://doi.org/10.47430/ujmr.1942.009
Sibanda, T. and Okoh, A. I. (2007). The challenges of overcoming antibiotic resistance: Plant extracts as potential sources of antimicrobial and resistance modifying agents. African Journal of Biotechnology, 6(25).
Sofowora, A. (1993). Screening plants for bioactive agents. Medicinal Plants and Traditional Medicinal in Africa. 2nd Ed. Spectrum Books Ltd, Sunshine House, Ibadan, Nigeria, 134-156.
Sosa, E. (2007). Experimental philosophy and philosophical intuition. Philosophical studies, 132(1), 99-107. https://doi.org/10.1007/s11098-006-9050-3
Tan, P.V., Enow-Orock, G.E., Dimo, T., Nyasse, B. and Kimbu, S.F. (2006). Evaluation of the anti-ulcer and toxicity profile of Aloe buettneri in laboratory animals. Afr. J. Trad. CAM. (3):8–20. https://doi.org/10.4314/ajtcam.v3i2.31152
US Centers for Disease Control and Prevention (2022). Facts about cyanide. Updated April 4, 2018. Accessed November 1, 2022. https://emergency.cdc.gov/agent/cyanide/basics/facts.asp
Vidaver, A. K. and Lambrecht, P. A. (2004). Bacteria as plant pathogens. The plant health Instructor, 1-11. https://doi.org/10.1094/PHI-I-2004-0809-01
Vucenik, I. and Shamsuddin, A.M. (2003). Cancer inhibition by inositol hexaphosphate (1p6) and inosotol from laboratory to the clinic. Journal of Nutrition. 133(11): 3774-3784. https://doi.org/10.1093/jn/133.11.3778S
Zinsou, V. A., Afouda, L. A. C., Zoumarou-Wallis, N., Pate-Bata, T., Dossou, L., Götz, M. and Winter, S. (2015). Occurrence and characterisation of Xanthomonas axonopodis pv. glycines, causing bacterial pustules on soybean in Guinea Savanna of Benin. African Crop Science Journal, 23(3): 203-210. ISSN 1021-9730/2015.
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Copyright (c) 2024 Ayokunle Oluwaseun Alejo, Ayodele Martins Ajayi, Benjamin Oluwole Akinyele
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