Effect of Oil Pollution on the Antioxidant Activity of the Methanolic Extracts of Polluted and Unpolluted Leaves of Hyptis suaveolens (L)
DOI:
https://doi.org/10.47430/ujmr.2381.010Keywords:
Hyptis suaveolens,, Anti-oxidants,, DPPH,, PollutantsAbstract
Hyptis suaveolens (L.) Poit, an aromatic weed known as pignut or daddoya-ta-daji in the Hausa language, has a longstanding history of usage as food and medicine in Kaduna state. This plant is interestingly found thriving in environments with or without oil pollution. In our modern industrial society, it's practically inevitable that these medicinal plants come in contact with oil contamination. This is largely because oil pipelines, often aged and susceptible to corrosion or illicit activities leading to leakages, are scattered across urban and rural settlements, influencing the plants' phytochemical composition. This study is focused on examining if oil pollution alters the antioxidant activity of this plant. An evaluation of the antioxidant properties of methanol extracts from Hyptis suaveolens (L.) leaves, both from polluted and unpolluted areas, was carried out using the 2,2-Diphenyl-1-Picrylhydrazyl-hydrate (DPPH) free radical scavenging assay. The outcomes showed that the extracts have significant (p < 0.05) concentration-dependent antioxidant activity. Yet, the unpolluted H. suaveolens leaves' methanol extract exhibited superior DPPH radical scavenging action compared to the extract from the polluted leaves. The median inhibitory concentrations (IC50) for the polluted and unpolluted leaves' methanol extracts were 35 µg/ml and 20 µg/ml respectively. Therefore, the contamination had a detrimental impact on the plant extract's antioxidant capacity.
Downloads
References
Al Mayyahi S.O.M (2018), Assessment of Heavy Metals Pollution in the Sediments Surrounding of Badra Oil Field, Wasit, Iraq. Plant Archives, 18(2): 1889-1894. e-ISSN : 2581 - 6063(online), ISSN: 0972 - 5210
Akimat, E.K., Omwenga, G.I., Moriasi, G.A., and Ngug, M.P. (2021). Antioxidant, Anti-Inflammatory, Acute Oral Toxicity, and Qualitative Phytochemistry of The Aqueous Root Extract of Launaea cornuta (Hochst. Ex Oliv. & Hiern.). Journal of Evidence-Based Integrative Medicine; 26: 1-12;https://doi.org/10.1177/2515690X211064585
Brand-Williams, W., Cuvelier, M.E., and Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Food Science and Technology; 28: 25 - 30.https://doi.org/10.1016/S0023-6438(95)80008-5
Al Mayyahi S.O.M (2018), Assessment of Heavy Metals Pollution in the Sediments Surrounding of Badra Oil Field, Wasit, Iraq. Plant.Archives, 18(2) : 1889-1894. e-ISSN : 2581 - 6063(online), ISSN: 0972 - 5210
Gjorgieva, D.A., Panovska, T.K., Ruskovska, T., Andonovska, K.B., Stafilov, T. (2013). Mineral mutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals. Physiology and Molecular Biology of Plants; 19(4): 499 - 507,https://doi.org/10.1007/s12298-013-0196-0
Cacique, A.P, Barbosa, E.S, Paulino de Pin,G, and Silverio, F. (2020). Maceration Extraction Conditions for Determining the Phenolic Compounds and the Antioxidant Activity of Catharantus roseus (L) G.Don. Science and Agrotechnology, 44: e017420,2020.http://dx.doi.org/10.1590/1412-7054202044017420
Guchu, B.M., Machocho, A.K., Mwihia, S.K. and Ngugi, M.P. (2020). In Vitro Antioxidant Activities of Methanolic Extracts of Caesalpinia volkensii Harms., Vernonia lasiopus O. Hoffm., and Acacia hockii De Wild. Hindawi Evidence-Based Complementary and Alternative Medicine. Volume 2020, Article ID 3586268, 10 pages.https://doi.org/10.1155/2020/3586268
Kozłowska, M., Scibisz, I., Przybył, J.L., Laudy, A.E., Majewska, E., Tarnowska, K., Małajowicz, J., Ziarno, M. (2022). Antioxidant and Antibacterial Activity of Extracts from Selected Plant Material. Applied Sciences; 12:9871.https://doi.org/10.3390/app12199871
León, J.A.M., Santisteban, A.G., Fuentes, D.P., Puebla, Y.G., and Rodríguez, E.T. (2018). In vitro anti-inflammatory activity of aqueous, ethanolic and ethereal extracts of rhizomes, leaves and stems of Anredera vesicaria. Journal of Analytical and Pharmaceutical Research; 7(2):459-461.https://doi.org/10.15406/japlr.2018.07.00266
Moriasi, G.A., Ireri, A.M., Nelson, E.M., and Ngugi, M.P. (2021). In vivo anti-inflammatory, anti-nociceptive, and in vitro antioxidant efficacy, and acute oral toxicity effects of the aqueous and methanolic stem bark extracts of Lonchocarpus eriocalyx (Harms.). Heliyon 7 (2021) e07145.https://doi.org/10.1016/j.heliyon.2021.e07145
Nakalembe, L., Kasolo, J.N., Nyatia, E., Lubega, A. and Bbosa, G.S. (2019). Analgesic and Anti-Inflammatory Activity of Total Crude Leaf Extract of Phytolacca dodecandra in Wistar Albino Rats. Neuroscience and Medicine, 10:259-271.https://doi.org/10.4236/nm.2019.103020
Oumarou M.K.; Younoussa L. and Nukenine (2018). Toxic effect of Chenopodium Ambrosoides, Hyptis Suaveolens and Lippia adoensis leaf methanol extracts and essential oils against fourth instar larvae of Anopheles gambiac (Diptera: culicidae). IJMR, 5(1): 61-62
Radwan, A.M., Reyad, N.F., Donia, E.R.M., and Ganaie, M.A. (2018). Comparative studies on the effect of environmental pollution on secondary metabolite contents and genotoxicity of two plants in Asir area, Saudi Arabia. Tropical Journal of Pharmaceutical Research; 17(8): 1599 - 1605.https://doi.org/10.4314/tjpr.v17i8.19
Rodríguez-Yoldi, M.J. (2021). Anti-Inflammatory and Antioxidant Properties of Plant Extracts. Antioxidants; 10: 921.https://doi.org/10.3390/antiox10060921
Runde, M., Kubmarawa, D., and Maina, H.M. (2015). Compositional analysis and anti-oxidant assessment of essential oil of some aromatic plants obtained from North-Eastern Nigeria. Research Journal of Chemical Sciences; 5(10): 7 - 12
Shekhar, T., and Anju, G. (2014). Antioxidant activity by DPPH Radical Scavenging Method of Ageratum conyzoides Linn, Leaves. Ameriacn Journal of Ethnomedicine; 1(4): 244 - 249
Ukom, A.N., Ndudim, S., and Nwanagba, L.N. (2019). Effect of fermentation periods on the nutrient quality and sensory acceptability of African Yam Beans (Sphenostylis sternocarpa) porridge. Nigerian Journal of Biotechnology; 36(1): 9 - 16,https://doi.org/10.4314/njb.v36i1.2
Yahaya M.F.; Kubmarawa D.; Yelwa J.M; and Runde M. (2018). Antioxidant and antimicrobial activity of essential oils extracted from aromatic plants. World Scientific News. An International Journal. WSN 111. 13-25
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 UMYU Journal of Microbiology Research
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
