Molecular Characterization of Agents of Cutaneous Leishmaniasis in Dutsin-Ma Local Government Area of Katsina State, Northwestern, Nigeria

Authors

  • Enimie, O. E. Department of Biotechnology, Nigerian Defence Academy, PMB 2109, Kaduna State, Nigeria
  • Umar, Y. A. Department of Biological Sciences, Nigerian Defence Academy, PMB 2109, Kaduna State, Nigeria
  • Sani, A. M. Department of Biotechnology, Nigerian Defence Academy, PMB 2109, Kaduna State, Nigeria
  • Nkechi, E. L. Department of Biotechnology, Nigerian Defence Academy, PMB 2109, Kaduna State, Nigeria
  • Sherifat, A-B, B. Department of Biotechnology, Nigerian Defence Academy, PMB 2109, Kaduna State, Nigeria

DOI:

https://doi.org/10.47430/ujmr.2493.008

Keywords:

Leishmaniasis, NTD, patients, species, molecular characterization

Abstract

Study’s Novelty Excerpt

  • This study provides novel insights into the epidemiology of cutaneous leishmaniasis (CL) in Dutsin-ma LGA, Katsina State, Nigeria, by identifying Leishmania major as the predominant species through molecular characterization.
  • The research uniquely combines molecular techniques with socio-demographic data, revealing a significant prevalence of CL lesions predominantly among male farmers.
  • These findings underscore the endemic nature of CL in the region and highlight specific occupational and demographic risk factors associated with the disease.

Full Abstract

Leishmaniasis is one of man's neglected tropical diseases (NTD), caused by a protozoan parasite of the Leishmania genus. The dixenous life cycle of Leishmania species consists of the vertebrate and vector stages resulting in the differentiation of metabolic processes, morphological forms, and remodelling of genes in the parasite. To identify the species of Leishmania responsible for cutaneous leishmaniasis (CL) in Dutsin-ma LGA of Katsina state, Nigeria, a purposive sampling technique was employed. Samples were collected from individuals with suspected CL lesions and subjected to molecular characterization to confirm the Leishmania species. Questionnaires were also used to gather socio-demographic and behavioral data. Molecular characterization involved amplifying the ITS rDNA genes and applying the restriction fragment length polymorphism (RFLP) technique. The results revealed that 85.7% of the individuals screened had lesions associated with Leishmania infection, with a higher prevalence in males (71.4%) compared to females (14.3%). Among the infected individuals, 71.46% were farmers, and one was a student. The lesions were predominantly found on the legs (57.1%) and hand/arm (28.6%), with no lesions detected on other body parts. The ITS rDNA genes were successfully amplified from six patients, confirming Leishmania major as the predominant species causing CL through RFLP analysis. In conclusion, CL caused by L. major is endemic in Dutsin-ma LGA, Katsina state, in the Northwestern zone of Nigeria

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References

Aghai-Maybodi, M., Eslami, G., Tohidfar, M., Fattahi Bafghi, A., Hosseini, S. S., Ahmadian, S. and Elloumi, M. (2018). Molecular Characterization of Clinical Leishmania major Isolates Harboring ITS1 Homology Similar to the One in Crithidia spp. Journal Of Isfahan Medical School, Volume 36(500), 1261-1266.

Al-Nahhas, S. A. and Kaldas, R. M. (2013). Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis. ISRN Parasitology,Volume 2013, Article ID 308726: 1-5. http://dx.doi.org/10.5402/2013/308726

Ben-Ahmed, K., Bouratbine, A., & El-Aroui, M. A. (2010). Generalized Linear spatial Models in epidemiology: A case study of zoonotic cutaneous leishmaniasis in Tunisia. Journal of Applied Statistics, Volume 37(1): 159-170. https://doi.org/10.1080/02664760802684169

Calvopiña, M. and Hashiguchi, R. A. Y. (2004). Epidemiology of leishmaniasis in Ecuador: current status of knowledge - a review. Memórias do Instituto Oswaldo Cruz, 99: 663-672. https://doi.org/10.1590/S0074-02762004000700001

Davies, C. R.,Wanos-Cuentos, E. A., Sharp, S. J., Canales, J., Leon, E., Alvarez, E., Roncal, N. and Dye, C. (1997). Cutaneous leishmaniasis in the Peruvian Andes: Factors associated with variabilityin clinical symptoms, response to treatment and parasites isolation rate. Clinical Infectious Diseases, Volume 25(2): 302-310. https://doi.org/10.1086/514535

Desjeux, P.(2001). The increase of risk factors for leishmaniasis worldwide. Transaction of the Royal Society for Tropical Medicine and Hygiene, Volume 95: 239-243. https://doi.org/10.1016/S0035-9203(01)90223-8

Desjeux, P. (2004a). Focus: Leishmaniasis. Nature Reviews Microbiology, Volume 2(9): 692-692. https://doi.org/10.1038/nrmicro981

Guerra-Silveira, F. and Abad-Franch, F. (2013). Sex bias in infectious disease epidemiology: patterns and processes. PLoS One, 8: e62390. https://doi.org/10.1371/journal.pone.0062390

Hijjawi N, Kanani K A, Rasheed M, Atoum M, Abdel-Dayem M, and Irhimeh M R. (2016). Molecular Diagnosis and Identification of Leishmania Species in Jordan from Saved Dry Samples. BioMed Research International, Volume 2016, Article ID 6871739. https://doi.org/10.1155/2016/6871739

Igbe, M., Duhlinska, D. and Agwale, S. (2006). Epidemiological Survey of Cutaneous Leishmaniasis in Jos East L.G.A. Of Plateau State Nigeria. The Internet Journal of Parasitic Diseases, Volume 4 (1), Pp 1-3.

Isa, A., Umar, Y, A. and Appah, J. (2017). Species Composition of Phlebotomine Sandfly (Diptera:Psychodidae) Vectors of Leishmaniasis in Katsina State, Northern Nigeria. International Journal of Scientific & Engineering Research, Volume 8 (11): 1786-1793.

Khalil, E. A. G., Khidir, S. A., Musa, A. M., Musa, B. Y., Elfaki, M. E. E., Elkadaru, A. M. Y. and El-Hassan, A. M. (2013). Post-kala-azar dermal leishmaniasis: a paradigm of paradoxical immune reconstitution syndrome in non-HIV/AIDS patients. Journal of Tropical Medicine, 2013. https://doi.org/10.1155/2013/275253

Laurent, T., Van der Auwera, G., Hide, M., Mertens, P., Quispe-Tintaya, W., Deborggraeve, S. and Dujardin, J. C. (2009). Identification of Old World Leishmania spp. by specific polymerase chain reaction amplification of cysteine proteinase B genes and rapid dipstick detection. Diagnostic Microbiology and Infectious Disease, Volume 63(2): 173-181. https://doi.org/10.1016/j.diagmicrobio.2008.10.015

Mandell, G. L., Bennett, J. E. and Dolin. R. (2010). Mandell, Douglas, and Bennett's principles and practice of infectious diseases. 7th edition (vols 1 and 2) Edited by Mandell GL, Bennett JE, and Dolin R. Churchill Livingstone. Pp 4028. ISBN 978-0-443-06839-3.

Mouttaki, T., Morales-Yuste, M., Merino-Espinosa, G., Chiheb, S., Hassan Fellah, Martin-Sanchez J. and Riyad, M. (2014). Molecular diagnosis of cutaneous leishmaniasis and identification of the causative Leishmania species in Morocco by using three PCR-based assays. Parasites & Vectors, Volume 7:420. https://doi.org/10.1186/1756-3305-7-420

Opara W.E.K. and Ameh I.G. (2005). Cutaneous leishmaniasis: A Report of its Treatment with Mectizan in Sokoto, Nigeria. Journal of Medical Sciences, Volume 5: 186-188. https://doi.org/10.3923/jms.2005.186.188

Reithinger, R., Dujardin, J. C., Louzir, H., Pirmez, C., Alexander, B. and Brooker, S. (2007). Cutaneous Leishmaniasis. Lancet Infectious Disease,Volume 7(9):581-596. https://doi.org/10.1016/S1473-3099(07)70209-8

Remadi L, Chargui N, Jime'nez M, Molina R, Haouas N, Gonza'lez E, et al. (2020) Molecular detection and identification of Leishmania DNA and blood meal analysis in Phlebotomus (Larroussius) species. PLoS Neglected Tropical Disease,Volume 14(3): e0008077. https://doi.org/10.1371/journal.pntd.0008077

Sakthianandeswaren, A., Foote, S. J. and Handman, E. (2009). The role of host genetics in leishmaniasis. Trends in parasitology, Volume 25(8), 383-391. https://doi.org/10.1016/j.pt.2009.05.004

Slami, G., Bafghi, A. F., Lotfi, M. H., Mirzaei, F., Ahmadi, S., Tajfirouzeh, A. A., Jafarizadeh, H., Pormazar, S. A. and Vakili, M. (2020). Isolation and molecular identification of Leishmania spp. agents in patients with cutaneous leishmaniasis in yazd province, endemic region of central Iran. Iran j public health, Volume49(5): 975-980. https://doi.org/10.18502/ijph.v49i5.3216

Sogand, A. (2017). The identification of new Leishmania using ITS1-rDNA gene. International journal of mosquito research, Volume 4(2): 44-51. Travi, B. L., Osorio, Y., Melby, P. C., Chandrasekar, B., Arteaga, L. and Saravia, N. G. (2002). Gender is a major determinant of the clinical evolution and immune response in hamsters infected with Leishmania spp. Infect Immun.,70(5): 2288-96. https://doi.org/10.1128/IAI.70.5.2288-2296.2002

Weigle, K. A., C. Santrich, F. Martinez, L. Valderrama, and N. G. Saravia. (1993). Epidemiology of cutaneous leishmaniasis in Colombia: environmental and behavioral risk factors for infection, clinical manifestations, and pathogenicity. J. Infect. Dis., 168:709-714. https://doi.org/10.1093/infdis/168.3.709

World Health Organization. (2020). Ending the neglect to attain the Sustainable Development Goals A road map for neglected tropical diseases 2021-2030. WHO; Geneva.

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Published

09-06-2024

How to Cite

Enimie, O. E., Umar, Y. A., Sani, A. M., Nkechi, E. L., & Sherifat, A-B, B. (2024). Molecular Characterization of Agents of Cutaneous Leishmaniasis in Dutsin-Ma Local Government Area of Katsina State, Northwestern, Nigeria. UMYU Journal of Microbiology Research (UJMR), 58–64. https://doi.org/10.47430/ujmr.2493.008