Phytochemical Analysis and Antifungal Activity of Leaves Extract of Vachellia seyal on Phytophthora infestants Isolated from rotten potatoes
DOI:
https://doi.org/10.47430/ujmr.2493.011Keywords:
Vachellia, Phytochemical/GC-MS Analysis, AntifungalAbstract
Study’s Novelty/Excerpt
- This study is novel as it provides the first detailed examination of the antifungal and phytochemical properties of methanolic extracts from Vachellia seyal leaves against Phytophthora infestans, a significant agricultural pathogen.
- By employing both GC-MS analysis and qualitative phytochemical screening, this research identifies a wide array of bioactive compounds, including terpenoids and flavonoids, which are linked to the extract's antifungal efficacy.
- Furthermore, the study highlights the superior inhibitory effect of the methanolic extract compared to ethyl acetate extracts, underscoring V. seyal's potential as a source of novel antifungal agents for managing fungal infections.
Full Abstract
Studies looking at the presence of potent antimicrobial compounds in plants have been sparked by the use of plants and their extracts as treatments for various illnesses. The study aimed to analyze the antifungal and phytochemical properties of a methanolic extract of Vachellia seyal leaves on P. infestant isolated from rotten potato tubers. Following drying, the leaves were extracted using methanol as the solvent using cold maceration and percolation. To check for the existence of bioactive components, the extract underwent GC-MS analysis and qualitative phytochemical screening. The antifungal inhibitory activity of the leaf extract was assessed using agar well diffusion techniques. The results of the phytochemical screening indicated the presence of secondary metabolites, including terpenoids, tannins, phenols, flavonoids, alkaloids, saponins, and steroids, in the leaves. Thirty-five (35) compounds were detected from the GC-MS study; based on their peak, six (6) were determined to be the most prevalent. These compounds include 9-Octadecenoic acid (Z)-, methyl ester (23.35%), Squalene (11.84%), 9,15-Octadecadienoic acid, methyl ester, (Z,Z)- (10.63%), Pentadecanoic acid, 14-methyl-, methyl ester (7.95%), (E)-9-Octadecenoic acid ethyl ester (7.19%) and Butylated Hydroxytoluene (1.85%). With a growth inhibition zone of 37mm, the results showed that the leaf extract of V. seyal was effective against P. infestant. The response of V. seyal may have been caused by the presence of phenolic compounds and acetic acid from the leaf extract. Additionally, methanolic crude extract was more effective than ethyl acetate extracts in controlling the activities of certain fungal pathogens. According to the study, V. seyal has antifungal activity and needs to be considered as a possible antifungal agent while looking for novel treatments for fungal infections.
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References
Aja, P.M., Okaka., A.N.C., Onu, P.M., Ibiam, U &Urako, .A.J. (2010). Phytochemical composition of Talinum triangulare (Water Leaf) Leaves. Parkistan Journal of Nutrition. 9 (6): pp.527-530.
Arbonnier, M. (2009). Arbres, arbustes et lianes des zones sèches d'Afrique de l'Ouest. Versailles Ed Quae, MHNH. 574.
Bodeker, G., Ghat, K.K.S., Burley, J.& Vantomme, P. (1997). Phytomedicinal forest harvest in the United States. Med Plants 11:147-58.
Bojaxa, A. R., Henry, J. &Roslie, S. (2010). Phytochemical and Pharmacognostic evaluation of some species in Fabaceae.International Journal of Biotechnology. 1(1): 12-15.
Brockwell, J., Searle, S.D. & Jeavons, A.C.(2005). Nitrogen fixation in acacias: an untapped resource for sustainable plantations, farm forestry and land reclamation. Australian Centre for International Agricultural Research (ACIAR). 132.
Chowdappa, P., Mohan Kumar, S. P., Sanjeev, S. & Singh, B. P. (2011). Integrated management of early and late blight of potato and tomato. ORP on leaf spot diseases series, 17.
Chowdappa, P., Nirmal Kumar, B. J., Madhura, S., Mohan Kumar, S. P., Myers, K. L., Fry, W. E. & Cooke, D. E. L. (2015). Severe outbreaks of late blight on potato and tomato in South India caused by recent changes in the Phytophthora infestans population. Plant Pathology, 64(1), 191-199. https://doi.org/10.1111/ppa.12228
Clement, B.A., Goff, C. M. & Forbes, T. D. A. (1997). Toxic amines and alkaloids from Acacia berlandieri. Phytochemistry. ; 46: 249–254.
Edeoga, H.O., Okwu, D.E. & Mbaebie, B.O. (2005). Phytochemical constituents of some Nigerian medicinal plants. A’fr.J. Biotechnol., 4(7): 685-688.
Fatou, D., Diegane, D., Agnieszka, K., Antoine, L. Q., Niokhor, B. & Dioumacor, F. (2015) Genetic and genomic diversity studies of Acacia symbionts in senegal reveal new species of mesorhizobium with a putative geographical pattern. J Plos One 10:1371-91.
Group, A.P. (2009). An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG III. Bot J Linn Soc. 161: 105-121.
Haridas, V., Higuchi, M., Jayatilake, G.S., Bailey, D., Mujoo, K., Blake, M.E., Arntzen, C.J. & Gutterman, J.U. (2001). Avicins: Triterpenoid saponins from Acacia victoriae (Bentham) induce apoptosis by mitochondrial perturbation. Proc. Natl. Acad. Sci. USA. ; 98:5821–5826.
Haverkort, A. J., Struik, P. C., Visser, R. G. F. & Jacobsen, E. (2009), "Applied biotechnology
Hubert, G.Y.J., Julienne, N., Charles, D.D., Daniel, F., Sandrine, P.T., Romain, F.F. &Henry, A.Z.P. (2013) Antifungal Potential and Phytochemical Analysis of Extracts from Seven Cameroonian Plants against Late Blight Pathogen Phytophthora infestans. International Journal of Current Microbiology and Applied Sciences, 2, 140-154.
Ikram, M.E., Eiman, S.E., Jihad, H.E. & Saad, M.H.A. (2016). Comparative studies of antioxidant and antimicrobial activities of Acacia seyal stem, stem wood and stem bark dry distillates. Am J PharmTech Res;6:440-50.
Jain, N., Sharma, M., Joshi, S. C., & Kaushik, U. (2018). Chemical composition, toxicity and antidermatophytic activity of essential oil of Trachyspermum ammi. Indian Journal of Pharmaceutical Sciences, 80(1), 135–142. https://doi.org/10.4172/pharmaceutical-sciences.1000338
Kamaraj, C. (2011). Larvicidal and repellent activity of medicinal plant extracts from Eastern Ghats of South India against malaria and filariasis vectors Asian Pac. J. Trop. Med.
Kubmarawa, D., Ajoku, G.A., Enwerem, N.M. & Okorie, D.A. (2007). Preliminary phytochemical and antimicrobial screening of 50 medicinal plants from Nigeria. Afr. J. Biotechnol. 6(14): 1690-1696
Lewis, G., Schrire, B., Mackinder, B.& Rico, L.(2013). Linear sequence of legume genera set in a phylogenetic context-a tool for collections management and taxon sampling. S Afr J Bot. 89: 76-84.
Levetin, E. (2004). An Atlas of fungal spores. Journal of Allergy and Clinical Immunology, 113(2): 366-368. https://doi.org/10.1016/j.jaci.2003.09.049
Mahesh, B. & Satish, S. (2008). Medicinal Plant against Plant and Human Pathogens. World Journal of Agricultural Sciences, 4 (5): 839-843.
Maher, T., Ahmad Raus, R., Daddiouaissa, D., Ahmad, F., Adzhar, N.S. & Latif, E.S.(2021).Mohammed, A. Medicinal Plants with Anti-Leukemic Effects: A Review. Molecules 26, 2741.
Mercy, K. A., Ijeoma, I., & Emmanuel, K. J. (2014). Anti-dermatophytic Activity of garlic ( Allium sativum ) extracts on some Dermatophytic fungi. International Letters of Natural Sciences, 19, 34–40. https://doi.org/10.56431/p-2o985a
Mekbib, S.B., Regnier, T.J.& Sivakumar, D.(2009). Evaluation of Ethiopian plant extracts, Acacia seyal and Withania somnifera, to control green mould and ensure quality maintenance of citrus (Citrus sinensis L.) Fruits. 64: 285-294.
Nowicki, M. (2013)."A simple dual stain for detailed investigations of plant-fungal pathogen interactions", Vegetable Crops Research Bulletin, 77: 61– 74.
Perez, C., Paul, M. & Bazerque, P. (1990). An antibiotic assay by the agar-well diffusion method. Acta. Biol. Med. Exp. 15:113-115.
Prabhahar. C., Saleshrani, K., Saranraj, P. & Tharmaraj, K. (2012). Studies on the antifungal activity of Turnera subulata and Acacia nilotica against pathogenic fungal pathogens. International Journal of Recent Scientific Research. 3(3):149 -154
Rathod, L. R. & Pawar, P. V. (2012). Antimicrobial activity of medicinal plant to control seed borne pathogen of soybean. Current Botany, 3(2): 10-12
Romeh, A.A. (2013). Phytochemicals from Ficus sycomorus L. leaves act as insecticides and acaricides. African Journal of Agricultural Research, 8(27), 3571–3579.
Sadiq, M.B., Hanpithakpong,W., Trning, J. & Anal, A.K. (2015). Screening of phytochemicals and in vitro evaluation of antibacterial and antioxidant activities of leaves, pods and bark extracts of Acacia nilotica (L.) Del. Ind. Crops Prod. 77, 873–882. https://doi.org/10.1016/j.indcrop.2015.09.067
Saleh, B., Hammoud, R. & Al-Mariri, A. (2015). Antimicrobial activity of Ficus sycomorus L.(Moraceae) leaf and stem-bark extracts against multidrug resistant human pathogens. Herba Polonica, 61(1), 39-49.
Seigler, D.S. (2003). Phytochemistry of Acacia sensu lato. Biochemical Systematics and Ecology, (31): 845– 873. https://doi.org/10.1016/S0305-1978(03)00082-6
Shinkafi, S. A. (2013). Antidermatophytic activities, Phytochemical screening and Chromatographic studies of Pergularia tomentosa L . and Mitracarpus scaber Zucc. (Leaves) Used in the Treatment of Dermatophytoses. Research Paper, 4(January), 29–37.
Shinkafi, S. A. (2014). Phytochemical analysis and chromatographic studies of Pergularia tomentosa L. and Mitracarpus scaber Zucc. Microbiology Research Journal International, 550-559.
Singh, R., Singh, S., Kumar, S. & Arora, S. (2007). Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn. Food Chem. Toxicol. ; 45:1216–1223.
Sofowora, A.(1993). Screening Plants for Bioactive Agents. In: Medicinal Plants and Traditional Medicinal in Africa. 2nd Ed. Spectrum Books Ltd, Sunshine House, Ibadan, Nigeria, pp. 134-156.
Thiombiano, A., Schmidt, M. & Dressler, S.(2012). Catalogue des plantes vasculaires du Burkina Faso. Boissiera: mémoires des Conservatoire et Jardin botaniques de la Ville de Genève. 65: 1-391.
Trease, G. E. &Evans, W.C. (1989).Pharmacognsy(13th ed) Baillere.Tindall London pp176-180.
Ushie, O.A., Onen, A.I., Ugbogu, O.C., Neji, P.A.&Olumide, V.B. (2016).Phytochemical Screening and Antimicrobial Activities of Leaf Extracts of Swieteniamacrophylla. Chem Search Journal 7(2): 64 – 69.
Wink, M. (2013). Evolution of secondary metabolites in legumes (Fabaceae). South Afr J Bot 89:164-75. https://doi.org/10.1016/j.sajb.2013.06.006
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