Evaluation of Phytochemical Constituents and Antimicrobial Activity of Calotropis procera Root Extract against some Pathogenic Microorganisms in Yola, Adamawa State, Nigeria

Authors

  • Shitu, A. S. Department of Microbiology, Faculty of Life Sciences, Modibbo Adama University, Yola, Adamawa State, Nigeria https://orcid.org/0000-0002-8131-9712
  • Salisu, Z. N. Faculty of Science, Department of Biological Sciences, Federal University Kashere, Gombe State, Nigeria
  • Johnson, A. A. Department of Microbiology, Faculty of Life Sciences, Modibbo Adama University, Yola, Adamawa State, Nigeria https://orcid.org/0000-0001-7586-5410

DOI:

https://doi.org/10.47430/ujmr.2493.023

Keywords:

Antimicrobial, Calotropis procera, Microorganisms, Phytochemical compounds, Root extracts

Abstract

Study’s Novelty/Excerpt

  • This study evaluates the antimicrobial potential of Calotropis procera root extracts, traditionally used in Nigerian medicine, by scientifically validating their therapeutic properties.
  • The research identified various phytochemical constituents, including tannin, saponin, alkaloids, phenolics, flavonoids, and reducing sugars, with terpenoids uniquely present in the aqueous extract.
  • The significant antimicrobial activity observed, particularly with the chloroform extract showing the largest inhibition zone against Staphylococcus aureus, underscores the potential of C. procera roots as a rich source of bioactive compounds for developing alternative treatments for infectious diseases.

Full Abstract

Calotropis procera has been traditionally used for medicinal purposes in Nigeria, with various parts of the plant utilized by traditional healers without scientific validation of their therapeutic properties. Therefore, this study sought to assess the phytochemical constituents and antimicrobial efficacy of root extracts from C. procera against pathogenic microorganisms. The extraction of C. procera roots was carried out using aqueous, chloroform, and n-hexane solvents, and the qualitative analysis of the phytochemical constituents was conducted following standard procedures. The antimicrobial activity of the extracts was evaluated against Staphylococcus aureus, Salmonella typhi, Klebsiella pneumoniae, Candida albicans, and Aspergillus flavus through the agar well diffusion method. Phytochemical screening identified the presence of tannin, saponin, alkaloids, phenolics, flavonoids, and reducing sugars in all extracts, with terpenoids exclusively found in the aqueous extract and cardiac glycosides absent in all extracts. The extracts demonstrated significant antimicrobial activity against all tested pathogens, with the chloroform extract exhibiting the largest inhibition zone (23.50±0.38 mm) against S. aureus, while the n-hexane extract displayed the smallest inhibition zone (6.38±0.52 mm) against A. flavus. Minimum inhibitory concentration (MIC) values were determined as 25 mg/ml for the chloroform extract against S. aureus and S. typhi, and 50 mg/ml for both aqueous and n-hexane extracts against K. pneumoniae and C. albicans. The minimum bactericidal concentration (MBC) was established at 100 mg/ml for S. aureus, S. typhi, and all extracts. The root extracts of C. procera exhibited promising antimicrobial effects against S. aureus and S. typhi, indicating its potential as a rich source of bioactive compounds and a viable alternative to antibiotics for treating infectious diseases.

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References

Akindele, P. O., Fatunla, O. A., Ibrahim, K. A., & Afolayan, C. O. (2017). Antibacterial and phytochemical screening of Calotropis procera leaf extracts against vancomycin and methicillin-resistant bacteria isolated from wound samples in hospital patients. Journal of Complementary and Alternative Medical Research, 1-14. https://doi.org/10.9734/JOCAMR/2017/30975

Al Sulaibi, M. A., Thiemann, C. and Thiemann T. (2020). Chemical constituents and uses of Calotropis procera and Calotropis gigantea–a review (Part I–the plants as material and energy resources). Open Chemistry Journal, 7:1–15. https://doi.org/10.2174/1874842202007010001

Al-Rowaily, S. L., Abd-ElGawad, A. M., Assaeed, A. M., Elgamal, A. M., El Gendy, A. E. N. G. and Mohamed T. A. (2020). Essential oil of Calotropis procera: comparative chemical profiles, antimicrobial activity, and allelopathic potential on weeds. Molecules, 25:5203. https://doi.org/10.3390/molecules25215203

Aly, A. A., Ali, H. G. M. and Eliwa, N. E. R. (2019). Phytochemical screening, anthocyaninnns and antimicrobial activities in some berries fruits. Journal of Food Measurement and Characterization, 13: 911-920. https://doi.org/10.1007/s11694-018-0005-0

Behbani, B. A., Nehjad, A. A., Hojjati, M., Vasiee, A. and Mehrnia, M. A. (2023). Identification of phytochemical, antioxidant, anticancer and antimicrobial potential of Calotropis procera leaf aqueous extract. Scientific reports, 13:14716. https://doi.org/10.1038/s41598-023-42086-1

Bello, S.A., Ayofe, T.A., Yakub, M.F., Jamiu, A.T. (2020). Comparative analysis of the antimicrobial potential of stem and fruit extracts of Calotropis procera. Pharmacognosy Research, 12:368-74. https://doi.org/10.4103/pr.pr_58_20

Clinical and Laboratory Standards Institute (2021). Performance standards for antimicrobial susceptibility testing, twenty-second informational supplement, CLSI, Document M100 S27, CLSI, Wayne, Pa, USA.

Elmarie, V. W. and Jonah, C.P. (2001).Purification and identification of active antibacterial component in Carpobrotus edulis L. Journal of Ethnopharmacology, 76: 87-91. https://doi.org/10.1016/S0378-8741(01)00197-0

Ezekwesili-Ofili, J. O., and Okaka, A. N. C. (2019). Herbal Medicines in African Traditional Medicine. In Chapter 10, IntechOpen.

Falana, M. B. and Nurudeen, Q. O. (2020). Evaluation of phytochemical constituents and in vitro antimicrobial activities of leaf extracts of Calotropis procera against certain human pathogens. Notulae Scientia Biologicae, 12(2): 208– 221. https://doi.org/10.15835/nsb12210699

Jamiu, A.T. and Bello, S.A. (2018). Biosynthesis of silver nanoparticles using azadirachta indica leaf extract and assessment of its antibacterial activity on some pathogenic enteric bacteria. International Journal of Novel Research in Life Science, 5:25 31.

Kareem, S. O., Akpan, I. and Ojo, O. P. (2008). Antimicrobial Activities of Calotropis Procera on Selected Pathogenic Microorganisms. African Journal of Biomedical Research, 11:105 110.

Kawo, A. H., Mustapha, A., Abdullahi, B. A., Rogo, L. D., Gaiya, Z. A., Kumurya AS (2009).Phytochemical properties and antibacterial activities of the leaf and latex extracts of Calotropis procera (Ait.F.). Bayero Journal of Pure and Applied Sciences, 2(1): 34-40. https://doi.org/10.4314/bajopas.v2i1.58453

Kitula, R.A. (2007). Use of medicinal plants for human health in Udzungwa Mountains Forests: A case study of New Dabaga Ulongambi Forest Reserve, Tanzania. Journal of Ethnobiology and Ethnomedicine, 3:7. https://doi.org/10.1186/1746-4269-3-7

Leonard G. O., Edeghagba, B., Abiola, O.M., Elijah, A.I. (2013). Antimicrobial activity of extracts of Jatropha curcas and Calotropis procera leaves against pathogenic isolates from motorcycle helmets in Lagos metropolis. International Journal of Current Microbiology and Applied Sciences, 2(12): 29.

Makhuvele, R., Naidu, K., Gbashi, S., Thipe, V.C., Adebo, O.A. and Njobeh, P.B. (2020). The use of plant extracts and their phytochemicals for control of toxigenic fungi and mycotoxins. Heliyon, 6(10):e05291. https://doi.org/10.1016/j.heliyon.2020.e05291

Marasini, B.P, Baral, P., Aryal, P., Ghimire, K.R., Neupane, S., Dahal, N., et al. (2015). Evaluation of the antibacterial activity of some traditionally used medicinal plants against human pathogenic bacteria. Biomedical Research Institute, 265425. https://doi.org/10.1155/2015/265425

Mehmood T., Arshad H., Nawaz S., Ullah A., Hafeez A. and Anwar F. (2020). Pharmaceutical potential and phenolics profiling of leaves and bark of Calotropis procera about extraction solvents. Pharmaceutical Chemistry Journal, 54 631–641. https://doi.org/10.1007/s11094-020-02250-7

Narayani, M., Johnson, M., Sivaraman, A., Janakiraman, N. (2012). Phytochemical and Antibacterial Studies on Jatropha curcas L. Journal of Chemical and Pharmaceutical Research, 4(5): 2639- 2642.

National Bureau of Statistics (2021). Reports for 2021, Federal government of Nigeria.

Nejhad, A.A., Behbahani, B.A., Hojjati, M., Vasiee, A. and Mehrnia, M. A. (2023). Identification of phytochemical, antioxidant, anticancer and antimicrobial potential of Calotropis procera leaf aqueous extract. Scientific Reports, 13:14716. https://doi.org/10.1038/s41598-023-42086-1

Nenaah, G. Antimicrobial activity of Calotropis procera Ait. (Asclepiadaceae) and isolation of four flavonoid glycosides as the active constituents. World Journal of Microbiology and Biotechnology, 29: 1255–1262 (2013). https://doi.org/10.1007/s11274-013-1288-2

Obeta, M. U., Ikeagwulonu, R. C., Ohanube, G. A. K., and Jwanse, I. R. (2020). Some Igbo Indigenous Plants with Anti-COVID-19 Properties. In Alternative Medicine Update. IntechOpen, 10-15.

Quazi, S., Mathur, K., Arora, S. and Wing, P. (2013). Calotropis procera: An overview of its phytochemistry and pharmacology. Indian Journal of Drugs, 1:63–69 (2013). https://doi.org/10.1155/2013/691372

Saadabi, A. M. A., Ali, N. M., Mohammed, H. I., Alsafi, F. N. and Mustafa, H. B. (2012). An in vitro Antimicrobial activity of Calotropis procera (Ait). R. Br. Extracts on certain groups of pathogenic microorganisms. Research Journal of Medical Sciences, 6 (1):13-17. https://doi.org/10.3923/rjmsci.2012.13.17

Wadhwani, B. D., Mali, D., Vyas, P., Nair, R. and Khandelwal, P. (2021). A review on phytochemical constituents and pharmacological potential of Calotropis procera. RSC Advances, 11:35854–35878. https://doi.org/10.1039/D1RA06703F

WHO. Antibiotics resistance. WHO; (2022). Accessed April 21, 2022 https://www.who.int/news-room/fact-sheets/detail/antibiotic-resistance

Yesmin, M. N., Uddin, S., Mubassara, S. and Akond, M.E. (2008). Antioxidant and antibacterial activities of Calotropis procera. AM-Eurasian Journal of Agric and Environmental Science, 4:550-553.

Yissa, T.D., Okunowo, W.O., Afolayan, R.I., Agboola, A.R., Lukman, H.Y., Suleiman, A., and Majiyebo, A.J. (2022). Phytochemical compositions and antimicrobial activity of leaf extracts of Calotropis procera against food spoilage microorganisms. AROC in Natural Products Research, 1(2): 36-46. https://doi.org/10.53858/arocnpr01023646

Zhang, Q., Lin, L. and Ye, W. (2018). Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine, 13: 20.

Zhu, Y., Huang, W.E., Yang, Q. (2022). Clinical perspective of antimicrobial resistance in bacteria. The opinion on recent clinic antibiotics abuse. Infection Drug Resistance, 15: 735- 746. https://doi.org/10.1093/jacamr/dlac103

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Published

29-06-2024

How to Cite

Shitu, A. S., Salisu, Z. N., & Johnson, A. A. (2024). Evaluation of Phytochemical Constituents and Antimicrobial Activity of Calotropis procera Root Extract against some Pathogenic Microorganisms in Yola, Adamawa State, Nigeria. UMYU Journal of Microbiology Research (UJMR), 9(3), 194–200. https://doi.org/10.47430/ujmr.2493.023