Determination of Antibiotic Resistance Patterns of Beta-Lactamase-Producing Escherichia coli Strains from Kindirmo in Nasarawa Town, Nasarawa State, Nigeria
DOI:
https://doi.org/10.47430/ujmr.2493.024Keywords:
Antibiotic Resistance, Beta-lactamase, E. coli, Kindirmo, Nasarawa State, NigeriaAbstract
Study’s Novelty/Excerpt
- This study examined the antibiotic resistance patterns of beta-lactamase-producing Escherichia coli in Kindirmo, a traditional fermented milk product, in Nasarawa town, Nasarawa State, Nigeria.
- It identifies the prevalence of beta-lactamase-producing E. coli in Kindirmo, highlighting a 5.92% contamination rate and revealing complete resistance to six antibiotics among the isolates.
- The study underscores the public health risks associated with consuming Kindirmo and calls for stringent hygiene measures during its production and sale to protect consumers.
Full Abstract
This study investigates the antibiotic resistance patterns of beta-lactamase-producing Escherichia coli in Kindirmo in Nasarawa town, Nasarawa State, Nigeria. Over five months, 169 samples were collected from four sampling points. Escherichia coli was isolated using the streak-plating method and identified through biochemical tests. All isolates tested positive for beta-lactamase production. The agar-disc diffusion technique assessed the beta-lactamase-producing E. coli strains' susceptibility to 10 antibiotics. Out of 169 samples, 10 were contaminated with E. coli, resulting in a prevalence of 5.92%. The isolates showed complete resistance to six antibiotics, while 70% and 50% demonstrated susceptibility to ofloxacin, pefloxacin, and gentamicin, respectively. Six antibiotic-resistance phenotypes were identified among the E. coli isolates. The presence of beta-lactamase-producing E. coli in Kindirmo suggests that consuming the product in the area poses a risk to consumers. Authorities should enforce basic hygiene requirements during production and sales to safeguard public health.
Downloads
References
Abike, T.O., Olufunke, O.A. and Oriade, K.D. (2015). The prevalence of multiple resistant Escherichiacoli serotypes in cow raw milk samples and traditional dairy products in Osun state, Nigeria. British Microbiology Research Journal, 5:117-125. https://doi.org/10.9734/BMRJ/2015/12868
Agersø, Y. and Aarestrup, F.M. (2013). Voluntary ban on cephalosporin use in Danish pig production has effectively reduced extended- spectrum cephalosporinase-producing Escherichia coli in slaughter pigs. Journal of Antimicrobial Chemotherapy, 68: 569–572. https://doi.org/10.1093/jac/dks427
Akarsh, K.L., Prejit, Asha, K., Vergis, J., Vinod, V.K. and Andrews, H.P. (2019). Occurrence and characterisation of extended-spectrum ß-lactamase producing E. coli and Salmonella species from raw milk samples in Wayanad District, Kerala, India. International Journal of Current Microbiology and Applied Sciences, 8(12): 2089-2098. https://doi.org/10.20546/ijcmas.2019.812.247
Badri, A.M., Ibrahim, I.T., Mohamed, S.G., Garbi, M.I., Kabbashi, A.S. and Arbab, M.H. (2018). Prevalence of extended spectrum beta lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae isolated from raw milk samples in Al Jazirah State, Sudan. Molecular Biology, 7(1):1000201. https://doi.org/10.4172/2168-9547.1000201
Bush K. and Jacoby, G.A. (2010). Updated functional classification of ß-lactamases. Antimicrobial Agents and Chemotherapy, 54(3):969-976. https://doi.org/10.1128/AAC.01009-09
Cheesbrough, M. (2010). District Laboratory Manual for Tropical Countries (Low Price Edition). Cambridge University Press, London. pp. 65-158.
Clinical and Laboratory Standards Institute (CLSI) (2016). Performance Standards for Antimicrobial Susceptibility Testing (26th ed.). CLSI Supplement M100S. Wayne PA, USA.
Dahms, C., Hubner, N.O., Kossow, A., Mellmann, A., Dittmann, K. and Kramer, A. (2015). Occurrence of ESBL-producing Escherichia coli in livestock and farm workers in Mecklenburg-Western Pomerania Germany. PLoS ONE, 10: e0143326. https://doi.org/10.1371/journal.pone.0143326
Dohmen, W., Bontem, M.J.M., Bos, M.E.M., van Marm, S., Scharringa, J., Wagenaar, J.A. and Heederik, D.J.J. (2015). Carriage of extended- spectrum β-lactamases in pig farmers is associated with occurrence in pigs. Clinical Microbiology and Infections, 21: 917–923. https://doi.org/10.1016/j.cmi.2015.05.032
Food and Drug Administration (FDA) of the United States. Centre for Food Safety and Applied Nutrition. http://www.fda.gov/educationresourcelibrary. Accessed on: March 14, 2023.
Liu, X., Liu, H., Wang, L., Peng, Q., Li, Y., Zhou, H. and Li, Q. (2018). Molecular characterisation of extended-spectrum beta-lactamase-producing multidrug resistant Escherichia coli from Swine in Northwest China. Frontiers in Microbiology, 9: 1756. https://doi.org/10.3389/fmicb.2018.01756
Gandra, S., Barter, D. M. and Laxminarayan, R. (2014). Economic burden of antibiotic resistance: How much do we really know? Clinical Microbiology and Infection, 20: 973–980. https://doi.org/10.1111/1469-0691.12798
Kamaruzzaman, E.A., Abdul Aziz, S., Bitrus, A.A., Zakaria, Z. and Hassan, L. (2020). Occurrence and characteristics of extended-spectrum ß-lactamase-producing Escherichia coli from dairy cattle, milk, and farm environments in Peninsular Malaysia. Pathogens, 9:1007. https://doi.org/10.3390/pathogens9121007
Khan, J., Musaddiq, M. and Budhlani, G.N. (2014). Antibiotic resistant of pathogenic bacteria isolation from milk and milk products in Akola city of Maharashtra. India Journal of Life Science, 4:59-62.
Liu, L., Oza, S., Hogan, D., Perin, J., Rudan, I., Lawn, J. E., Cousins, S., Matters, C. and Black R.E. (2015). Global regional, and national causes of child mortality in 2000-2013, with projections to inform post-2015 priorities: an updated systematic Analysis. The Lancet, 385: 430440. https://doi.org/10.1016/S0140-6736(14)61698-6
Lucey, J.A. (2015). Nutritional and food science, raw milk consumption, risks and benefits. Available at https//www.Cdr. wisc.ed/sites/default /files/PDFs/director corner/Raw milk consumption Risks. Benefits accessed on March 14, 2023. https://doi.org/10.1097/NT.0000000000000108
Nahar, A., Islam, A.K.M.A, Islam, M.N., Khan, M.K., Khan, M.S., Rahman, A.K.M.A. and Alam, M.M. (2023). Molecular characterisation and antibiotic resistance profile of ESBL-producing Escherichia coli isolated from healthy cow raw milk in smallholder dairy farms in Bangladesh. Veterinary World, 16(6):1333-1339.https://doi.org/10.14202/vetworld.2023.1333-1339
National Bureau of Statistics (2009). Federal Republic of Nigeria, 2006 population census. http://nigeriastatgov.ng/connection/pop2006.pdf. Accessed on March 14, 2023.
Rashid, M., Kotwal, S.K., Malik, M.A and Singh, M. (2013). Prevalence, genetic profile of virulence determinants and multidrug resistance of E. coli isolates from foods of animal origin. Veterinary World, 6(3):139-142. https://doi.org/10.5455/vetworld.2013.139-142
Reuben, C.R., Okolocha, E.C., Bello, M. and Tanimu, H. (2013). Occurrence and antibiogram of Escherichia coli O157:H7 in locally fermented milk (Nono) sold under market conditions in Nasarawa State, Nigeria. International Journal of Science and Research, 2(2):591-598.
van den Bunt, B., Liakopoulos, D., Mevius, J., Geurts, Y., Fluit, A.C., Bonten, M.J.M., Mughini-Gras, L. and Pelt van, W. (2017). ESBL/AmpC-producting Enterobacteriaceae in households with children of preschool age: Prevalence, risk factor and co-carriage. Journal of Antimicrobial Chemotherapy, 72: 589–595. https://doi.org/10.1093/jac/dkw443
Veenemans, J., Overdevest, I.T., Snelders, E., Willemsen, I., Hendriks, Y., Adesokan, A., Doran, G., Bruso, S., Rolfe, A. and Petterson, A. (2014). Next generation sequencing for typing and detection of resistance genes: Performance of a new commercial method during an outbreak of extended spectrum beta-lactamase- producing Escherichia coli. Journal of Clinical Microbiology, 52: 2454–2460. https://doi.org/10.1128/JCM.00313-14
Wu, G., Day, M.J., Mafura, M.T., Nunez-Garcia, J., Fenner, J.J., Sharma, M., Essen-Zandbergen, A., Rodrι´guez, I., Dierikx, C. and Kadlec, K. (2013). Comparative analysis of ESBL- positive Escherichia coli isolates from animals and humans from the UK, The Netherlands and Germany. PLoS ONE, 8: e75392. https://doi.org/10.1371/journal.pone.0075392
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 UMYU Journal of Microbiology Research (UJMR)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
