Screening for Plasmid Mediated Antibiotic Resistance among Multi Drug Resistant Bacteria Isolated from Patients with Lower Respiratory Tract Infections Attending some Hospitals in Kano Metropolis
DOI:
https://doi.org/10.47430/ujmr.2493.034Keywords:
Lower Respiratory Tract Infections, MDR Bacteria, Plasmids, KanoAbstract
Study’s Novelty/Excerpt
- This study investigates the prevalence and plasmid-mediated resistance of multidrug-resistant (MDR) bacteria isolated from patients with lower respiratory tract infections (LRTIs), revealing significant findings pertinent to public health.
- The research demonstrates a high prevalence of MDR isolates (20%) and highlights the substantial role of plasmids, with 67.6% of MDR isolates containing plasmids, and 80% losing drug resistance post-plasmid curing.
- These results underscore the necessity for treatment options based on antibiotic susceptibility testing and continuous plasmid profiling to combat plasmid-mediated resistance effectively, marking a critical advancement in the management of LRTIs.
Full Abstract
Lower Respiratory tract infections (LRTIs) are among the serious infections in humans that have been worsening by the emergence of drug resistant bacteria and aggravated by plasmids transfer. The study aimed to screen Multidrug-resistant (MDR) bacteria isolated from patients with LRTIs for the presence of Plasmid. Sputum samples (400) were collected from patients with LRTIs and processed using standard microbiological procedures to isolate lower respiratory tract bacteria. The identified isolates were subjected to antibacterial susceptibility testing using the disc diffusion method. Multidrug resistant isolates were further subjected to plasmid curing using Acridine orange and the cured isolates were tested for loss of drug resistance. Among the 400 samples, 185 (46.2 %) harbored significant bacterial growth, with 83 (44.9%) Gram-positive and 102 (55.1%) Gram-negative bacteria. Bacteria isolated include Escherichia coli (29), Klebsiella pneumonia (46), Moraxella catarrhalis (13), Pseudomonas aerugonosa (14), Staphylococcus aureus(24) and Streptococcus pneumoniae (59). A higher infection rate was recorded in males (46.99%) and patients aged 41-50 (52.59%). The highest resistance was exhibited by Klebsiella pneumoniae (93%) against cefuroxime, followed by Streptococcus pneumoniae against oxacillin (75%). Of the 185 isolates, 37 (20%) were MDR, out of which 25 (67.6%) isolates had Plasmids. Following curing, 20 (80%) of the isolates were cured, and only 5 (20%) retained their plasmids. Most importantly, Escherichia coli and Klebsiella pneumonia were found to be sensitive to levofloxacin, gentamicin, and imipenem but retained their resistance to Cefuroxime, while Streptococcus pneumoniae was found to be sensitive to levofloxacin only with the highest resistance to oxacillin, doxycycline, and erythromycin. The study establishes a high prevalence of MDR isolates among LRTIs, with some exhibiting plasmid-mediated resistance, and therefore recommends treatment options to be solely based on antibiotic susceptibility testing and continuous plasmid profiling to detect plasmid-mediated resistance to enable appropriate drug administration.
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References
Agbagwa, O. E., Otokunefor, T. V. and Frank-Peterside, N. (2012). Plasmid profile analysis of bacteria isolated from selected honey samples in Nigeria. Journal of Animal Science Advances, 2: 338-343.
Ahmad, J. N., Yadava, S., and Ahmad, S. (1993). Elimination of R factors among Escherichia coli strains with special reference to norfloxacin. Indian Medical Journal, 7( 5): 115–122.
Akindele, P. O. and Afolayan, C. O. (2017). Plasmid profile of multidrug resistant bacteria isolated from wound swabs from hospital patients in Akure, Nigeria. Asian Journal of Medicine and Health, 2 (3): 1–13. https://doi.org/10.9734/AJMAH/2017/30973
Akingbade O. A., Ogiogwa, J. I., Okerentugba, P. O., Innocent-Adiele, H. C.and Nwanze, J.C. (2012). Prevalence and antibiotic susceptibility pattern of bacterial agents involved in lower respiratory tract infections in Abeokuta, Ogun States, Nigeria. Report Opinion, 4: 25-30.
Amadi, A. N., Dan- Hassan, M. A., Okoye, N. O., Ejiofor, I. C. and Aminu, T. (2013). Studies on Pollution Harzards of Shallow Hand-Drug Wells in Erena and Environs, North- Central Nigeria. Environment and Natural Resources Research, 3 (2): 69- 77. https://doi.org/10.5539/enrr.v3n2p69
Ayobola, E. D., Oscar, W. O and Ejovwokoghene, E. F. (2021). Occurrence of plasmid mediated fluoroquinolone resistance genes amongst enteric bacteria isolated from human and animal sources in Delta state, Nigeria. AIMS Microbiology, 7 (1): 75–95,. https://doi.org/10.3934/microbiol.2021006
Brown, T. A. (2000). Essential Molecular Biology, a Practical approach. Oxford University Press.2nd Edition Pp 69-102. https://doi.org/10.1093/oso/9780199636426.001.0001
Cheesbrough, M. (2006). District Laboratory Practice in tropical Countries. Part II, 2 (Ed). Cambridge University Press, New York, PP: 65-67. https://doi.org/10.1017/CBO9780511543470
CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 30th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute.
David, P. C., and Nanette, J. P. (2013). Molecular Biology: International Union of Biochemistry and Molecular Biology, Academic Press Cell, 2nd Edition edition,.
Fair, R. J. and Tor, Y. (2014). Antibiotics and bacterial resistance in the 21st century. Perspectives in Medicinal Chemistry, 6: 25–64. https://doi.org/10.4137/PMC.S14459
Freifeld, D. (1983). Molecular Biology: A Comprehensive Introduction to Prokaryotes and Eukaryotes, Science Books international, Van Nostrand Reinhold Company.
Grohmann, E., Muth, G., Espinosa, M. (2003). Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev, 67:277–301. https://doi.org/10.1128/MMBR.67.2.277-301.2003
Henderson, R. and Sundaren, T. (1982). Cluster Sampling to assess immunization coverage : a review of experience with a simplified sampling method. Bulletin of the world Health Medicine. Corpus ID: 45908102
Ineta, B. E. L., Madu, E. P., Abdulhadi, A. S. A., Ibrahim, H. I. M. (2002). Antibiotic susceptibility and plasmid profile of clinical isolates of Escherichia coli. Biomed Res 2018; 29:3303-3310. 29 Integrated pharmacology, 2nded: Edinburgh : Mosby. https://doi.org/10.4066/biomedicalresearch.29-18-927
Kalgo, Z.M., Amin, B.M., Muhammed, B. and Saka, H.K. (2023). Prevalence and risk factors for Lower Respiratory Tract Infection: a Multicenter study, at Kebbi State, Nigeria. International Journal of Advanced Health Science and Technology, 3, (1):https://doi.org/10.35882/ijahst.v3i1.170
Kumar, R., Lalit, D., Ritvik, A., Siddhartha, S. and Kathryn, E. L. (2021). Incidence, risk factors and viral aetiology of community acquired acute lower respiratory tract infection among older adults in rural North India. J. Glob. Health, 11: 04027. https://doi.org/10.7189/jogh.11.04027.
Lakhundi, S. and Zhang, K. (2018). Methicillin-resistant Staphylococcus aureus: molecular characterization, evolution, and epidemiology. Clinical Microbiology Reviews, 31 (4). https://doi.org/10.1128/CMR.00020-18
Livak, K. J.(1984). Organisation and mapping of a sequence on the Drosophila melanogaster X and Y Chromosomes that is transcribed during spermatogenesis. Genetics, 107 (4): 611-634. https://doi.org/10.1093/genetics/107.4.611
Munita, J. M. and Arias, C. A. (2016). Mechanisms of Antibiotic Resistance. Microbiology Spectrum, 4(2). https://doi.org/10.1128/microbiolspec.VMBF-0016-2015
Nikaido, H. (2009). Multidrug resistance in bacteria. Annual Review of Biochemistry, 78 (1): 119–146. https://doi.org/10.1146/annurev.biochem.78.082907.145923
Nowicki, J., Murray, M.T. (2020). Bronchitis and Pneumonia. Textbook of Natural Medicine; Elsevier: Amsterdam, The Netherlands, pp. 1196–1201. https://doi.org/10.1016/B978-0-323-43044-9.00155-2
Oleghe, P. O., Odimegwu, D. C., Udotia, E. and Esimore, C. O. (2011). Multidrug resistance bacterial isolates recovered from herbal medicinal preparations in southeastern setting, Nigeria. Journal of Rural and Tropical Public Health, 10: 70–75.
Panda, S., Prema, N. B. and Ramani, T. V. (2012). Lower Respiratory tract infection. Bacteriological Profile and antibiogram pattern. Int J Cur Res Rev, 4: 149-155
Patwardhan, R. B., Dhakephalkar, P. K., Chopade, B. A., Dhavale, D. D. and Bhonde, R. R. (2018). Purification and characterization of an active principle, lawsone, responsible for the plasmid curing activity of Plumbagozeylanica root extracts. Frontiers in Microbiology, 9: 2618. https://doi.org/10.3389/fmicb.2018.02618
Peterson, E. and Kaur, P. (2018). Antibiotic Resistance Mechanisms in Bacteria: Relationships Between Resistance Determinants of Antibiotic Producers, Environmental Bacteria, and Clinical Pathogens. Frontiers in Microbiology. 9: 2928. https://doi.org/10.3389/fmicb.2018.02928
Prestinaci, F., Pezzotti, P. and Pantosti, A. (2015). Antimicrobial resistance: a global multifaceted phenomenon. Pathogens and Global Health, 109 (7): 309–318. https://doi.org/10.1179/2047773215Y.0000000030
Reboucas, R. H., Viana de Sousa, O., Sousa, L. A., Roger, F. V., Carvalho, P. B., Fernandes, R. H. V. (2010). Antimicrobial resistance profile of Vibrio species isolated from marine shrimp farming environments (Litopenaeusvannamei) at Cear’a, Brazil. Environ Res 2011; 111:21-24. https://doi.org/10.1016/j.envres.2010.09.012.
Shahidullah, M. S., Yusuf, M. A., Khatun, Z., Ara, U. K. M. N. and Mitul, M. T. (2012). Antibiotic sensitivity pattern of bacterial isolates from different clinical specimens: experience at NICVD, Dhaka. Cardiovascular Journal, 5 (1): 67–72. https://doi.org/10.3329/cardio.v5i1.12276
Taura, D. W., Hassan, A., Yayo, A. M. and Takalmawa, H. (2013). Bacterial isolates of the respiratory tract infection and their current sensitivity pattern among patients attending Aminu Kano Teaching Hospital Kano Nigeria. Int. Res. J. Microbiol. 4(9): 226-231.
Torres, A., Cilloniz, C., Niederman, M. S., Menéndez, R., Chalmers, J.D., Wunderink, R. G. and van der Poll, T. (2021). Pneumonia. Nat. Rev. Dis. Primers 7, (25). https://doi.org/10.1038/s41572-021-00259-0
Troeger, C., Blacker, B., Khalil, I.A., Rao, P.C., Cao, J. (2018). Estimates of the global, regional, and national morbidity, mortality, and aetiologies of lower respiratory infections in 195 countries, 1990–2016: a systematic analysis for the Global Burden of Disease Study 2016. The Lancetinfectdis,18 (11): 1191-1210.
Winokur, P. L., A. Brueggemann, A. and DeSalvo, D. L. (2000). Animal and human multidrug-resistant, Cephalosporin-Resistant Salmonella Isolates expressing a plasmid-mediated CMY-2 AmpC β-Lactamase. Antimicrobial Agents and Chemotherapy, 44 (10): 2777–2783. https://doi.org/10.1128/AAC.44.10.2777-2783.2000
Woerther, P. L., Burdet, C., Chachaty, E. and Andremont, A. (2013). A Trends in Human fecal carriage of extended-spectrum B-lactamases in the community: towards the globalization of CTX-M. Clinical Microbiology Reviews. 26 (4): 744-758. https://doi.org/10.1128/CMR.00023-13
Yang, H., Chen, H., Yang, Q., Chen, M. and Wang, H. (2008). High prevalence of plasmid-mediated quinolone resistance Genesqnrandaac (6)-Ib-crin clinical isolates of Enterobacteriaceae from nine teaching hospitals in China, AntimicrobialAgentsandChemotherapy, 52 (12): 4268–4273. https://doi.org/10.1128/AAC.00830-08
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