Molecular Docking of Phytocompounds from Mentha Piperita Leaf Extract as Promising Inhibitory Agents against Candida Albicans’s Glucosamine-6-Phosphate Synthase

Shafiu Aliyu; Abdulkadir Magashi Magaji; Muhammed Yusha’u; Aminu Aliyu; Saifullahi Gambo; Abubakar Kabiru; Sanusi Sani

doi:10.47430/ujmr.2493.038

Authors

Shafiu Aliyu Department of Microbiology, Federal University Gusau, Zamfara State, Nigeria https://orcid.org/0009-0006-2789-240X
Abdulkadir Magashi Magaji Department of Microbiology, Bayero University Kano, Nigeria
Muhammed Yusha’u Department of Microbiology, Bayero University Kano, Nigeria https://orcid.org/0000-0002-4327-7451
Aminu Aliyu Department of Microbiology, Federal University Gusau, Zamfara State, Nigeria https://orcid.org/0000-0002-3074-4065
Saifullahi Gambo Department of Microbiology, Federal University Gusau, Zamfara State, Nigeria https://orcid.org/0009-0005-9996-0208
Abubakar Kabiru Department of Microbiology, Federal University Gusau, Zamfara State, Nigeria https://orcid.org/0009-0002-7072-746X
Sanusi Sani Department of Microbiology, Federal University Gusau, Zamfara State, Nigeria https://orcid.org/0009-0000-2565-8587

DOI:

https://doi.org/10.47430/ujmr.2493.038

Keywords:

Molecular Docking, Phytocompounds, Mentha piperita, Candida albicans, Chromatography, Retention Factor RF

Abstract

Study’s Novelty/Excerpt

This study identifies and assesses the active phytochemical components of Mentha piperita (peppermint) leaf extract that inhibit the glucosamine-6-phosphate synthase activity of Candida albicans.
Utilizing advanced techniques such as HPLC-DAD and Bioautography Agar Overlay, the research successfully isolated bioactive compounds including rutin, chlorogenic acid, neohesperidin, rosmarinic acid, and eriodictyol.
The identification of these specific compounds and their inhibitory effects on a key enzyme in Candida albicans underscores the potential of Mentha piperita as a source of novel antifungal agents.

Full Abstract

The Mentha piperita plant, also known as peppermint, is a perennial aromatic herb grown throughout most of the world and has long been utilized in traditional medicine. It is a member of the Lamiaceae family. This study aimed to assess and determine the active phytochemical components of Mentha piperita leaf extract that inhibit the glucosamine-6-phosphate synthase activity of Candida albicans. The plant materials were collected from Janyau, Gada Biyu area along Sokoto Road, Gusau, Zamfara state, Nigeria, and identified at the herbarium section of the Biological science Department, Federal University Gusau. The plant leaf was extracted using the soxhlet method and analyses the phytochemical constituents' presence. To identify the bioactive compounds in the extracts of Mentha piperita, thin layer chromatography (TLC) was used on glass slides coated with silica gel (0.2mm Kiesel-gel 60 F254, Merck). The resulting fraction was then analyzed using HPLC-DAD analysis and the Bioautography Agar Overlay Technique. Phytochemical analysis results showed the presence of flavonoids, glycosides, phenol, tannins, saponins, and alkaloids as secondary metabolites. Thin layer chromatography (TLC) separation of aqueous extracts provided one compound with an R_fvalue of 0.52, while methanol and n-hexane extract showed two compounds, each with R_fvalues of 0.79 and 0.74, 0.72 and 0.70. As bioautography shows, the most bioactive component among the five components is M1. In conclusion, the bioactive constituents identified by high-performance liquid chromatography are rutin, Chlorogenic acid, Neohesperidin, Rosmarinic acid, and Eriodictyol.

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Molecular Docking of Phytocompounds from Mentha Piperita Leaf Extract as Promising Inhibitory Agents against Candida Albicans’s Glucosamine-6-Phosphate Synthase

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