Molecular Docking of Phytocompounds from Mentha Piperita Leaf Extract as Promising Inhibitory Agents against Candida Albicans’s Glucosamine-6-Phosphate Synthase
DOI:
https://doi.org/10.47430/ujmr.2493.038Keywords:
Molecular Docking, Phytocompounds, Mentha piperita, Candida albicans, Chromatography, Retention Factor RFAbstract
Study’s Novelty/Excerpt
- This study identifies and assesses the active phytochemical components of Mentha piperita (peppermint) leaf extract that inhibit the glucosamine-6-phosphate synthase activity of Candida albicans.
- Utilizing advanced techniques such as HPLC-DAD and Bioautography Agar Overlay, the research successfully isolated bioactive compounds including rutin, chlorogenic acid, neohesperidin, rosmarinic acid, and eriodictyol.
- The identification of these specific compounds and their inhibitory effects on a key enzyme in Candida albicans underscores the potential of Mentha piperita as a source of novel antifungal agents.
Full Abstract
The Mentha piperita plant, also known as peppermint, is a perennial aromatic herb grown throughout most of the world and has long been utilized in traditional medicine. It is a member of the Lamiaceae family. This study aimed to assess and determine the active phytochemical components of Mentha piperita leaf extract that inhibit the glucosamine-6-phosphate synthase activity of Candida albicans. The plant materials were collected from Janyau, Gada Biyu area along Sokoto Road, Gusau, Zamfara state, Nigeria, and identified at the herbarium section of the Biological science Department, Federal University Gusau. The plant leaf was extracted using the soxhlet method and analyses the phytochemical constituents' presence. To identify the bioactive compounds in the extracts of Mentha piperita, thin layer chromatography (TLC) was used on glass slides coated with silica gel (0.2mm Kiesel-gel 60 F254, Merck). The resulting fraction was then analyzed using HPLC-DAD analysis and the Bioautography Agar Overlay Technique. Phytochemical analysis results showed the presence of flavonoids, glycosides, phenol, tannins, saponins, and alkaloids as secondary metabolites. Thin layer chromatography (TLC) separation of aqueous extracts provided one compound with an Rf value of 0.52, while methanol and n-hexane extract showed two compounds, each with Rf values of 0.79 and 0.74, 0.72 and 0.70. As bioautography shows, the most bioactive component among the five components is M1. In conclusion, the bioactive constituents identified by high-performance liquid chromatography are rutin, Chlorogenic acid, Neohesperidin, Rosmarinic acid, and Eriodictyol.
Downloads
References
Abd Rashed, A., Rathi, D. N. G., Ahmad Nasir, N. A. H., & Abd Rahman, A. Z. (2021). Antifungal properties of essential oils and their compounds for application in skin fungal infections: Conventional and nonconventional approaches. Molecules, 26(4), 1093. https://doi.org/10.3390/molecules26041093
Alqethami, A., and Aldhebiani, A. Y. (2021). Medicinal plants used in Jeddah, Saudi Arabia: phytochemical screening. Saudi Journal of Biological Sciences, 28(1), 805-812. https://doi.org/10.1016/j.sjbs.2020.11.013
Anwar, F., Abbas, A., Mehmood, T., Gilani, A. H., & Rehman, N. U. (2019). Mentha: A genus rich in vital nutra‐pharmaceuticals—A review. Phytotherapy Research, 33(10), 2548-2570. https://doi.org/10.1002/ptr.6423
Bipin K., and Kathmandu (2019) Phytochemical and Antibacterial Analysis of Mentha piperita (Peppermint)
Cajka, T., Smilowitz, J. T., & Fiehn, O. (2017). Validating quantitative untargeted lipidomics across nine liquid chromatography–high-resolution mass spectrometry platforms. Analytical chemistry, 89(22), 12360-12368. https://doi.org/10.1021/acs.analchem.7b03404
Cresset Flare© software, version 4.0 (https://www.cresset-group.com/flare/).
Demetrio, L., Valle j.r., Juliana janet, m., puzon, Esperanza, c., Cabrera and windell L. (2016). thin layer chromatography-bioautography and gas chromatography-mass spectrometry of antimicrobial leaf Extracts from Philippine piper betle L. against multidrug-resistant Bacteria. Evidence based complementary and alternative medicine. https://doi.org/10.1155/2016/4976791
El Jurdi, K., Al Souky, N., Melquist, S. J., Al-Hadeethi, D., Salyers, W. J., & El Zein, M. (2023). S682 Efficacy of Peppermint Oil in Irritable Bowel Syndrome: A Systematic Review and Meta-Analysis. Official journal of the American College of Gastroenterology| ACG, 118(10S), S498-S499. https://doi.org/10.14309/01.ajg.0000952368.73973.08
Farooq Anwar, F. A., Ali Abbas, A. A., Tahir Mehmood, T. M., Gilani, A. H., and Najeeb-ur Rehman, N. U. R. (2019). Mentha: a genus rich in vital nutra-pharmaceuticals-a review. https://doi.org/10.1002/ptr.6423
Fayed, M. A. (2019). Mentha Piperita L.-A promising dental care herb mainly against cariogenic bacteria. Universal Journal of Pharmaceutical Research, 4(3), 33-38. https://doi.org/10.22270/ujpr.v4i3.271
Gosh, A., Dashi, B.K., Roy, A., Mandala, B. and Chand, G. (2008). Antibacterial activity of some medicinal plant extracts. Journal of Natural Medicine; 62: 259-262.
Gurib-Fakim, A. (2006). Medicinal plants: traditions of yesterday and drugs of tomorrow. Molecular aspects of Medicine, 27(1), 1-93. https://doi.org/10.1016/j.mam.2005.07.008
Haris N. G., Abubakar A. I., Aisha S. B. and Sa’adu U. (2021) Phytochemical Constituent and Antifungal Potentials of Mentha piperita (Pepper Mint) Leaf Extract on Urophathogenic Candida albicans. Dutse Journal of Pure and Applied Sciences; 7(1): 1-13
Haris, N.G., Taura, D.W., Abubakar, A.I., Yahaya, S.M., Ahmed, I., Aisha, S.B. (2017). Phytochemical screening and antibacterial activity of Menthapiperita (pepper mint) leaves extracts on urophatogenic Escherichia coli. Journal of Microbial Research; 3(2): 51 – 58
Kanoma, M. S., & Abdulkadir, M. (2022). The impact of future climate change on water availability in Gusau, Zamfara State, Nigeria. Dutse J. Pure Appl. Sci, 7, 144-154. https://doi.org/10.4314/dujopas.v7i4a.15
Kouri, G., Tsimogiannis, D., B ardouki, H., &Oreopoullou, V. (2007).Extraction and analysis of antioxidant components from Origanum dictamnus. Innovative Food Science and Emerging Technologies, 8 (2), 155-162. 2007. https://doi.org/10.1016/j.ifset.2006.09.003
Malika, Y. L., Thilinika, D., Janendra, M. D., Saman, E., Herath, S. & Wijayasiri, W. (2017).Physiological traits determining tolerance to intermittent drought in Capsicum annum complex.Scientia Horticulture, 246 (2019) 21-33. https://doi.org/10.1016/j.scienta.2018.10.047
Mayorga, P., Pérez, K.R., Cruz, S.M., Caceres, A. (2010). Comparison of bioassays using the anostracan crustaceans Artemia salina and Thamnocephalus platyurus for plant extract toxicity screening. Brasil Journal of Pharmacy; 20: 897-903. https://doi.org/10.1590/S0102-695X2010005000029
Mfengwana. P.M.A and Mashele.S.S. (2016). Antimicrobial activity screening of philenoptera violacea (Klotzsch) schrire and xanthocercis zambesiaca (baker) dumaz-Le-Grand. Journal of pharmaceutical sciences and research; 8(10): 1132-1135
Mutlu-Ingok, A., Devecioglu, D., Dikmetas, D. N., Karbancioglu-Guler, F., & Capanoglu, E. (2020). Antibacterial, antifungal, antimycotoxigenic, and antioxidant activities of essential oils: An updated review. Molecules, 25(20), 4711. https://doi.org/10.3390/molecules25204711
Naiman, A. K., Dominic, M. P., Mark, R. S. Lauren, B. R., Lauren, B. S. Dale, W. E. Arthur, F. K. & Charles, H. M. (2016). Moderate-to-vigorous activity, indices of cognitive control, and academic achievement in preadolescents.The Journal of Pediatrics, 1 (173), 136-142. https://doi.org/10.1016/j.jpeds.2016.02.045
Pramila D.M., Xavier R., Marimuthu K., Kathiresan S., Khoo M.L., Senthil M., Sathya K. and Sreeramanan S. (2012). “Phytochemical analysis and antimicrobial potential of methanolic leaf extract of peppermint (Mentha piperita: Lamiaceae)”. Journal of Medicinal Plants Research; 6(2), 331-335 https//doi.10.5897/JMPR11.1232
Prasathkumar, M., Anisha, S., Dhrisya, C., Becky, R., & Sadhasivam, S. (2021). Therapeutic and pharmacological efficacy of selective Indian medicinal plants–a review. Phytomedicine Plus, 1(2), 100029.. https://doi.org/10.1016/j.phyplu.2021.100029
Raja, N. S. (2021). Epidemiology, risk factors, treatment and outcome of Candida bloodstream infections because of Candida albicans and Candida non‐albicans in two district general hospitals in the United Kingdom. International Journal of Clinical Practice, 75(1), e13655. https://doi.org/10.1111/ijcp.13655
Ram, S., More-Adate, P., Tagalpallewar, A. A., Pawar, A. T., Nagar, S., and Baheti, A. M. (2023). An in-silico investigation and network pharmacology based approach to explore the anti-breast-cancer potential of Tecteria coadunata (Wall.) C. Chr. Journal of Biomolecular Structure and Dynamics, 1-12. https://doi.org/10.1080/07391102.2023.2252091
Shi, L., Wen, Z., Song, Y., Wang, J., & Yu, D. (2022). Computational investigation of potent inhibitors against SARS-CoV-2 2′-O-methyltransferase (nsp16): structure-based pharmacophore modeling, molecular docking, molecular dynamics simulations and binding free energy calculations. Journal of Molecular Graphics and Modelling, 117, 108306. https://doi.org/10.1016/j.jmgm.2022.108306
Silva, H. (2020). A descriptive overview of the medical uses given to Mentha aromatic herbs throughout history. Biology, 9(12), 484. https://doi.org/10.3390/biology9120484
Taura, M. G., Muhammed, A. A. & Alqahtani, D. O. (2021). Prevalence of congenital heart disease among patients with down syndrome in Southwestern Saudi Arabia. Annal of African Medicine, 20 (4), 265-269. 2012. https://doi.org/10.4103/aam.aam_57_20
Tsakni, A., Chatzilazarou, A., Tsakali, E., Tsantes, A. G., Van Impe, J., & Houhoula, D. (2023). Identification of Bioactive Compounds in Plant Extracts of Greek Flora and Their Antimicrobial and Antioxidant Activity. Separations, 10(7), 373. https://doi.org/10.3390/separations10070373
Zengin, G., Stefanucci, A., Rodrigues, M. J., Mollica, A., Custodio, L., Aumeeruddy, M. Z., & Mahomoodally, M. F. (2019). Scrophularia lucida L. as a valuable source of bioactive compounds for pharmaceutical applications: In vitro antioxidant, anti-inflammatory, enzyme inhibitory properties, in silico studies, and HPLC profiles. Journal of Pharmaceutical and Biomedical Analysis, 162, 225-233.https://doi.org/10.1016
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 UMYU Journal of Microbiology Research (UJMR)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
