Development of New Media for Production of Bacteriocin from Weissella cibaria man1
DOI:
https://doi.org/10.47430/ujmr.2492.027Keywords:
Antimicrobial, Antimicrobial Resistance, Lactic acid bacteria, biosynthesisAbstract
Study’s Excerpt:
• A novel, cost-effective medium was developed to enhance bacteriocin production from Weissella cibaria man1.
• New media were formulated using cheap, locally sourced ingredients like apple, cashew, and tiger nut.
• Bacteriocin production in the APC medium was higher and cheaper than commercial MRS broth.
• Optimal pH (5-6) significantly enhanced bacteriocin yield, improving its potential for medicinal use.
• This study offers a sustainable approach to bacteriocin production, aiding antibiotic resistance control.
Full Abstract:
According to the World Health Organization, antibiotic resistance is one of the top ten global health challenges. Bacteriocins from lactic acid bacteria are widely considered viable alternatives to antibiotics. However, one of the obstacles to bacteriocin commercialization is low yield. In order to circumvent this challenge, it is essential to enhance our understanding of the factors that impact bacteriocin production. Therefore, a study aimed at developing cost-effective media for bacteriocin production from Weissella cibaria man1 was carried out. Media were developed from cheap indigenously sourced materials. Bacteriocin production was measured by agar well diffusion assay. Multiple cost-effective media for the efficient production of bacteriocin from Weissella cibaria man1 were developed. It was discovered that culture media had significant effect (p ˂ 0.05) on the production of bacteriocin by Weissella cibaria man1. One of the newly developed media, APC, produced the highest level of bacteriocin. The APC medium produced bacteriocin at a level significantly higher than the commercial medium, MRS broth. Moreover, bacteriocin production in 6 additional, newly developed media namely, DAT, BAE, DAC, CAN, PIN, RIG was as high as that of MRS broth. Bacteriocin synthesis by Weissella cibaria man1 was greatly influenced by the starting pH of the APC culture medium. Furthermore, optimum level of bacteriocin was observed at pH values of 5 and 6. The industrial production of bacteriocin from bacteriocinogenic lactic acid bacteria for use in medicinal applications will be substantially facilitated as a result of these discoveries.
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Copyright (c) 2024 Samson Baranzan Wayah, Koshy Philip, Amos Alvan
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