Antibiotic Susceptibility Profile and Prevalence of AmpC among Clinical Bacterial Isolates obtained From Northwestern Nigeria
DOI:
https://doi.org/10.47430/ujmr.2162.010Keywords:
Antibiotics,, Bacteria,, Isolates,, AmpC,, Northwest,, Nigeria,, MDRAbstract
Antibiotic Resistance is spreading all over the world conferring multiple resistant in the treatment and management of life threatening infections. Cephalosporins are antibiotics prescribed daily for a wide variety of infections in Nigerian hospitals. The production of bla-AmpC enzymes by many Enterobacteriaceae conferred resistance to such class of antibiotics. The aim of the study is to determine the antibiotic susceptibility and prevalence of AmpC among clinical bacterial isolates obtained from Northwestern Nigeria. A total of 1000 clinical bacterial isolates were collected from seven states of north western Nigeria and were screened Phenotypically for AmpC production using Disk Approximation test. Antibiotic sensitivity test was performed according to clinical and laboratory standard Institutes guidelines (CLSI). Acinetobacter baumanii, Serratia liquefaciens and Providence sp showed 100% resistance to third generation cephalosporin antibiotics as well as Levofloxacin, Impinem and Tigercycline, moderate susceptibility was observed with Colistin, followed by Proteus Mirabilis (85%) resistance to Ceftizoxime. Tigercycline and Colistin were the most active drugs against the Isolates. The prevalence of AmpC production among the isolates showed that Acinetobacter baumanii, Aeromonas sp., Providence sp., Serratia liquefaciens and Citrobacter freundi had the highest prevalence of 100% each. Enterobacter clocae had least value of 50%. Similarly, high Multi-Drug Resistance (MDR) 100% was shown by A. baumannii, Aeromonas sp, Providence sp and S. liquefaciens with least values from Staphylococcus aureus (33.1%). The occurrence of AmpC was higher among male patients with prevalence rate of 54.1%, p-value >0.001.The occurrence of these resistance conferring enzymes was chronologically sensitive as its prevalence is more pronounced among patients aged >60 years (64.5%) with least value among patients aged 21-30 years (23.1%), (p-value>0.001). The trend in the prevalence of AmpC production and MDR resistance among the states of Northwestern Nigeria is in the following order: Sokoto˃Kaduna˃Katsina˃Kebbi>Kano˃Jigawa˃Zamfara. The results from this study implied that, AmpC production is on the increase in Northwestern Nigeria and that the spread of these resistance conferring enzymes among bacterial isolates is an issue of public health concerned. Therefore, proper monitoring and surveillance for proper prevention and infection control may limit the further spread of these isolates.
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References
Abdallah. M., Alhababi. R.,Alqudah. N., Aldyyat.B., and Alharthy A., (2018). First report of carbapenems-resistance Providencia stuartii in Saudi Arabia. J. New Microbes and New infections. Vol. 26 Nov. 2018. Pages 107-109.
https://doi.org/10.1016/j.nmni.2018.05.009
https://doi.org/10.1016/j.nmni.2018.09.007
Abdelrahman A., Mona A., Heba. R.,Abdelhafiz. H., and Engy. A., (2020). Antibiotic resistance and Extended-spectrum β- lactamase in Escherichia coli isolates from imported 1-day-old chicks, ducklings, and turkey pouts. Journal Vet World. pg 1037-1044. Epub 2020 Jun 10.Doi:10.14202/vetworld.2020.
https://doi.org/10.14202/vetworld.2020.1037-1044
Agouri SR., (2014). Genetic Characterization of MBL Positive Pseudomonas and Enterobacteriaceae. Cardiff University publishers (2014) pg 12-18, school of medicine, Cardiff University United Kingdom. Corpus ID; 83537201. Orca-Mwe.cf.Ac.uk.
Anderson.D J.,Kelly P.,Sandra. I., Berríos-Torres.D., Bratzler.W., Patchen.E., Dellinger. LG., Ann-Christine. N.,Lisa. S., Deborah. S.Y., Lisa. L., Maragakis. K., and Keith.S.,(2014): Strategies to Prevent Surgical Site Infections in Acute Care Hospitals: 2014 J Infection Control & Hospital Epidemiology, Issue S2: A Compendium of Strategies to Prevent Healthcare-Associated Infections in Acute Care Hospitals: 2014 Updates , Vol;35pp. S66 - S88September 2014 ,. DOIhttps://doi.org/10.1017/S0899823X00193869.
https://doi.org/10.1017/S0899823X00193869
Atieh. S.M., Babak S., Abbas. T., Ardakani. A., Moravveji. M.E., Mostafa.H.R., andMansoor.N., (2015). Multidrug-resistance Escherichia coli andKlebseilla pneumoniae isolated from Patients in Kashan Iran. Jundishapur Journal of Microbiology 8(10),2015.
https://doi.org/10.5812/jjm.27517
Bokaeian. M., Shahram. S.Z., Morteza. S.B., and Alireza A.M.(2014). Frequency of PER, VER, SHV, TEM and CTX-M Genes in Resistant strains of Pseudomonas aureginosa producing extended spectrum β-lactamases. Jundishapur J. microbial. 2014 Dec. 8:8(1): e13783. Doi: 10.5812?jjm.13783. ecollection 2015 Jan.
https://doi.org/10.5812/jjm.13783
Critchley. I. A.,Cotroneo. N.,Pucci. M., and Mendes. R., (2019).The burden of antimicrobial resistance among urinary tract isolates of Escherichia coli in the United States in 2017. J PloS One2019 Dec 10;14(12)https://doi.org/10.1371/journal.plonSOne. e0220265
https://doi.org/10.1371/journal.pone.0220265
Dancer. S., Raeside. J., and Boothma. M., (2002). Environmental organisms from different hospital wards. British Journal of Infection Control, vol.3,4:pp.10-14. Aug 1 2002.
https://doi.org/10.1177/175717740200300405
Duwadi. K., Sujan. K., Sanjib. A., and Sanjeep. S., (2020). Bacterial Ethiology of wound Exudates in Tertiary care Cancer Patients and Antibiogram of the Isolates. Infectious Diseases: Research and Treatment 13:1-8 august 2020. Doi:10,1177/1178633720952077.
https://doi.org/10.1177/1178633720952077
Egbule.O.S., and E.E. Odih.E.E., (2020). Prevalence of Extended-Spectrum Beta-Lactameses (ESBLs)and Metallo-Beta-lactamases (MBLs) Among Healthy and Hospitalized ChildreninAbraka and Eku Communities, Delta State, Nigeria. Nigeria journal of Basic and Applied Science 9June, 2020), 28(1):07-14. DOI: http://dx.doi.org/10.4314/injbas.v28i1.2.
https://doi.org/10.4314/njbas.v28i1.2
Fair.R.J and Tor. Y., (2014). Antibiotics and Bacterial Resistance in 21st Century. J PerspectMedicin Chem.2014; 6:25-64. Doi: 10.4137/PMC.S14459.
https://doi.org/10.4137/PMC.S14459
Flor. Y. R-C, Adriana.C.,Moreno.F., Francisco. M.D., Josee. H., and Alma. L.G.,(2018). An evaluation of Multidrug-resistant Escherichia coli isolates from Aguascalients Mexico: cross-sectional study. J. Ann.Clin. Microb 22018 July. 24; 17(1)34. Doi: 10.1186/s12941-018-0286-5.
https://doi.org/10.1186/s12941-018-0286-5
Fouch.S.,(2015). Molecular epidemiological study of extended spectrum beta lactamase (ESBL) Producing bacteria from teaching and district hospitals within Hampshire UK. https/ethos.bl.uk/orderDetails.do?uin+uk.bl.ethos.692939.
Gashe. F., Eshetu. M., Mekidim. M., andGemechu. Z., (2018). Antimicrobial resistance Profile of Different Clinical Isolates against Third- Generation Cephalosporins. Journal of Pharmaceutics. Volume 2018, Article ID 5070742, 7pages, 2018. https://doi.org/10.1155/2018/5070742.
https://doi.org/10.1155/2018/5070742
Grovera. N., Brig. A.K., Sahnib. C.,and Bhattacharya. S (2012). Therapeutic challenges of ESBLS and Ampc beta-lactamase producers in a tertiary care center. Medical Journal Armed Forces India (2013), 4-10, 69(1).
https://doi.org/10.1016/j.mjafi.2012.02.001
Gunjan.G., Vibhor.T., and Purva. M., (2014). Detection of AmpC β-Lactamases in gram-negative bacteria. Journal of laboratory physicians 6(01), 001-006, 2014.
https://doi.org/10.4103/0974-2727.129082
Hadi. M., and Mohammad. R., (2008)
Prevalence of extended-spectrum β-lactamase-producing Escherichia coli in a tertiary care hospital in Tehran, IranInternational Journal of Antimicrobial Agents (2008). 13(2).
Kapil A. (2005). The challenge of Antibiotic resistance need to contemplate. The Indian journal of Medical Research. Published 1 febuary 2005; 121(2):83-91. PMID 15756040.
Kiran. D.,Sujan. K., Sanjib. A., and Sanjeep.S., (2020). Bacterial Ethiology of wound Exudates in Tertiary care Cancer Patients and Antibiogram of the Isolates. Infectious Diseases: Research and Treatment 13:1-8 august 2020. Doi:10,1177/1178633720952077.
Kotra. LP.,Haddad. J., Liano-Sotelo.B., and Shabriar. M., (2002). Aminoglycosides modified by resistance enzymes display diminished binding to the bacterial ribosomal aminocl-tRNA site. J Chemistry & Biology 9 (4), 455-463, 2002- Elsevier.
https://doi.org/10.1016/S1074-5521(02)00125-4
Levy. C., de La Rocque. F., and Cohen. R., (2009). Epidemiology of pediatric bacterial meningitis in France Médecine et Maladies Infectieuses, (2009), 39(7-8)
https://doi.org/10.1016/j.medmal.2009.03.006
Mofolorunsho.K. C., Hannah. O., Ocheni. R..Aminu. F., Cornelius. A., Omatola. O., and Olowonibi. O., (2021).Prevalence and antimicrobial susceptibility of extended-spectrum beta lactamases-producing Escherichia coli and Klebsiella pneumoniae isolated in selected hospitals of Anyigba, Nigeria. J African Health Sciences (2021)Vol. 21 No. 2 (2021)
https://doi.org/10.4314/ahs.v21i2.4
O'Andrea. M.M., Fraziano. M.,Thaller. M.C., and Rossolili. G.M.,(2019). The urgent need for Novel Antimicrobial agents and strategies to fight Antibiotic Resistance. J. Antibiotics8 (4),254,2019.
https://doi.org/10.3390/antibiotics8040254
O'Neill..J., (2016). Tackling drug-resistance infections globally: final report and recommendations. Publisher; Government of the United Kingdom 2016. Analysis and policy Observatory (APO).
Okoche. D., Asiimwe. B.B., Katabazi.F.A.,Kato. L., Najjuka.C.F .,(2015). Prevalence and Characterization of Carbapenem-Resistant Enterobacteriaceae Isolated from Mulago National Referral Hospital, Uganda. Journal.pubmed. PLoS One. 2015
https://doi.org/10.1371/journal.pone.0135745
(8): e0135745 2015 Aug 18;10(8):e0135745. doi: 10.1371/.
https://doi.org/10.1371/journal.pone.0135745
Pitout. J.J.,Laupland.K.B., and Church. D.L.,(2008). Community-onset extended-spectrum β-lactamase (ESBL) producing Escherichia coli: Importance of international travel. Journal of Infection (2008) 57(6) 441-448. ISSN: 0163-4453
https://doi.org/10.1016/j.jinf.2008.09.034
Prestinaci. F., Pezzotti.P., and Pantoshi. A.(2015). Antimicrobial resistance: a global multifaceted phenomenon. J Pathog Glob Health.October, 2015;109(7):309-318. Doi:10.1179/2047773215Y.0000000030
https://doi.org/10.1179/2047773215Y.0000000030
Ramirez.C.F., Adriana. C., Moreno.F., Francisco. M.D.,Josee. H., Alma. L., and Guerrero.B., (2018). An evaluation of Multidrug-resistant Escherichia coli isolates from Aguascalients Mexico: cross-sectional study. J. Ann.Clin. Microb 22018 July. 24;17(1)34. Doi:10.1186/s12941-018-0286-5.
https://doi.org/10.1186/s12941-018-0286-5
Saarwar. S., Tarique. S., Waris. U.,and Anwaar. A.K., (2019). Cephalosporin resistance in community acquired Spontaneous Bacterial Peritonitis. Pakistan Journal of Medical Sciences 35(1),4,2019.
https://doi.org/10.12669/pjms.35.1.17
Sebastian. S., Tom. A. Annie.B., Joyal. A., and Joshy. M., (2021). Antibiotic resistance in Escherichia coli isolates from poultry environment and UTI patients in Kerala, India: A comparison study. J comparative Immunology, Microbiology and Infectious Diseases 2021), 75. DOI1 0.1016/j.cimid.2021.101614
https://doi.org/10.1016/j.cimid.2021.101614
WHO's first global report on antibiotic resistance reveals serious, worldwide threat to public health (2014). Saudi Medical Journal 2014 vol.35(7)
World Health Organization (WHO) (2014). ANTIMICROBIAL RESISTANCE Global Report on surveillance 2014.VI.04. SECTION. Survillance of antimicrobial drug resistance in diseases-specific programmes..pg 77-169
World Health Organization (WHO) (2017). Fight Antimicrobial Resistance- it's in your hands; call from the World Health Organization for 5th may 2017 academic. Oup
Yusuf. I., Arzai. A.H., Umah. A., Magaji. N., Salis. A., Tukur. M., Haruna. M., and Hamid. K.M., (2011). Prevalence of extended spectrum beta lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumoniae in tuberculosis patients in Kano, Nigeria.
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