Determination of Antibacterial Activity of Psidium guajava Leaf Extract against Bacteria Isolated from Mobile Phones of Umaru Musa Yar’adua University, Katsina Community
DOI:
https://doi.org/10.47430/ujmr.2161.032Keywords:
Mobile phones, contaminants, Antibacterial activity, Psidium guajava, antibacterialAbstract
Mobile phones are increasingly becoming one of the indispensable accessories of professional and social daily life, although constant handling and usage in various environmental conditions makes it fomite. Thus, antibacterial activity of Psidium guajava leaf extract was evaluated against bacteria isolated from mobile phones within Umar Musa Yar’adua University, Katsina community. A total of sixty (60) mobile phones were randomly swabbed, twenty (20) each, from hawkers, students, and staff of the University community. These were used as candidates for isolation of bacterial contaminants using standard protocols. Variable concentrations (500mg/ml, 300mg/ml and 100mg/ml) of aqueous extract of the Psidium guajava were prepared and tested against mobile phones bacterial isolates using agar well diffusion method. Preliminary phytochemical screening revealed the presence of flavonoids, cardiac glycosides, saponins, tannins and terpenoids. Staphylococcus aureus (39.6%), Escherichia coli (29.7%), Klebsiella sp. (18.8%), Proteus sp. (8.3%) and Pseudomonas aeruginosa (4.2%) were the bacterial contaminants isolated and identified from the mobile phones. Aqueous leaf extract of the plant displayed promising antibacterial activity at 500mg/ml against all the isolates, with average zones of inhibition of 25.0 mm for S. aureus, Proteus sp., P. aeroginosa and 24mm, 6.0mm for E. coli and Klebsiella sp. respectively. The use of Psidium guajava leaf extract as candidate for production of antibacterial agent which can be used to disinfect mobile handsets is suggested.
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Akintobi, O. A., Onoh, C. C., Ogele, J. O., Idowu, A. A., Ojo, O. V. and Okonko, I. O. (2013). Antimicrobial activity of Zingiber officinale (ginger) extract against some selected pathogenic bacteria. Nature and Science, 11(1): 7 - 15.
Akinyemi, K. O., Audu, D. A. and Akintoye, O. C. (2009). The potential role of mobile phones in the spread of bacterial infections. Journal of Infectious Diseases, 3(8): 628 - 632.
https://doi.org/10.3855/jidc.556
Al-Abdalall, A. H. (2010). Isolation and identification of microbes associated with mobile phones in Dammam in eastern Saudi Arabia. Journal of Family and Community Medicine, 1 (11)
https://doi.org/10.4103/1319-1683.68783
Brady, R. R., Wasson, A., String, I., Mc-Allister, C. and Damani, N. N (2006). Is your phone bugged? The incidence of bacteria known to cause nosocomial infection in health care workers. Journal of Hospital Infections, 62(1): 123-125.
https://doi.org/10.1016/j.jhin.2005.05.005
Bukar, A., Mukhtar, M. D., and Hassan, A. S. (2009). Phytochemical screening and screening of antimicrobial activity of Senna siamea on Pseudomonas aeruginosa. Bayero University Journal of Pure and Applied Sciences, 2(1): 139 - 142.
https://doi.org/10.4314/bajopas.v2i1.58528
Chesebrough, M. (2001). Medical Laboratory Manual for Tropical Countries. Volume 11, Second Edition, University Press, Cambridge, Great Britain, 377pp.
Cockerel, franklin R., et al (2012). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard - ninth edition. CLSI. P. 12. ISBN 1-56238-784-7
Deeni, Y. and Hussain, H. (1991). Plants in Kano ethno medicine: Screening for antimicrobial activity. Pharmaceutical Biology 29(1): 15-56.
https://doi.org/10.3109/13880209109082849
Demello, M. T. and Genan, S. O. (1999). Inhibition of Staphylococcus aureus by aqueous Gioba extracts. Journal of Ethnopharmacology 68(1-3): 103-108.
https://doi.org/10.1016/S0378-8741(99)00048-3
Enass, G. S. (2015). Isolation, Identification and Determination Antimicrobial Susceptibility of Bacteria Isolated from Mobile Phones of Student. Journal of University of Anbar for Pure Science, 9(3): 6-9.
https://doi.org/10.37652/juaps.2015.127544
Famurewa, O. and David, O. M. (2009). Cellphones: A medium of transmission of bacterial pathogens. World Rural Observations 1(2): 69-72.
Farhana, J. A., Hossain, M. D. and Mowlah, A. (2016). Antibacterial effects of Guava (Psidium guajava) extracts against food borne pathogens. International Journal of Nutrition and Food Sciences, 6(1): 1-5 Gondar, E. (2015). Isolation and characterization of bacteria from mobile phones of students and employees at University of Gondar Ethiopia. Bulletin of Pharmaceutical Research, 5(3): 96-100.
https://doi.org/10.11648/j.ijnfs.20170601.11
Kawo, A. H. and Musa, A. M. (2013). Enumeration, isolation and antibiotic susceptibility profile of bacteria associated with mobile cellphones in a university environment. Nigerian Journal of Basic and Applied Sciences, 21(1): 39 - 44.
https://doi.org/10.4314/njbas.v21i1.6
Kenneth, E., Paul, T., Istifanus, N., Uba, U., Rejoice, A., Victor, O. and Mohammed, S. (2017). Phytochemical analysis and antibacterial activity of Psidium guajava L. leaf extracts. GSC Biological and Pharmaceutical Sciences, 1(2): 13-19.
https://doi.org/10.30574/gscbps.2017.1.2.0024
Koscova, J., Hurnikova, Z. and Pistl, J. (2018). Degree of bacterial contamination of mobile phones and computer keyboard surfaces and efficacy of disinfection with chlorhexidine digluconate and triclosan to its reduction. International Journal of Environmental Research and Public Health, 15(10): 2238.
https://doi.org/10.3390/ijerph15102238
Offor, C. E. (2015). Phytochemical and proximate analysis of P guajava leaves. Journal of Research in Pharmaceutical science, 2(1): 5 - 7.
Olayemi, A. B., and Opaleye, F. I (1999). Antibiotic resistance among Coliform bacteria isolated from hospital and urban waste waters. World Journal of Microbiology and Biotechnology, 6(1): 285 - 288.
https://doi.org/10.1007/BF01201298
Pandey, A. and Shweta, E. (2011). Antifungal properties of P guajava leaves against fruit and fruits against various pathogens. Journal of Pharmaceutical and Biomedical Science, 13(13): 1 - 6.
Pooja D. Gupta, Tannaz J. Birdi (2017). Development of botanicals to combat antibiotic resistance. Journal of Ayurveda and integrative medicine, 8(1): 266 - 275.
https://doi.org/10.1016/j.jaim.2017.05.004
Sofowora, A. (1993). Medicinal Plants and Traditional Medicine in Africa. Spectrum Books, Nigeria. Second Edition, 10 - 15.
Suganya, S., and Sumarthy, V. (2012). Isolation and identification of bacteria from covered and uncovered mobile phones. International Journal of Environmental Sciences, 3(1): 44 - 54.
Uboh, F. E., Okon, I. E. and Ekong, M. B. (2010). Effect of aqueous extract of Psidium guajava leaves on liver enzymes, histological integrity and hematological indices in rats. Gastroenterology Research 3(1): 3.
https://doi.org/10.4021/gr2010.02.174w
Ummasalma Aliyu Saulawa and Yakubu Muhammad (2017). Antibacterial activity of root and leaves extracts of Senna occidentalis. Katsina journal of natural and applied sciences, 6(1): 69 - 74
Uthman, L., Sleiman, A. and Abdel-Massih, R. M. (2019). Antimicrobial Activity of Polyphenol and Alkaloids in Middle Eastern Plants. Frontiers and Microbiology, 10(1): 911.
https://doi.org/10.3389/fmicb.2019.00911
Vieira, R. H. S. F., Rodrigues, D. P., Gonclaves, F. A., Menezes, F. G. R., Aragao, J. S. and Sousa O. U. (2001). Microbial effect of medicinal plant extracts (Psidium guajava Linn and Carica papaya Linn) upon bacteria isolated from fish muscle and known to induce diarrhea in children. Review in St Medical Tropical Sau Paulo 43: 145-148.
https://doi.org/10.1590/S0036-46652001000300005
Yusha'u, M., Bello, M. and Sule H. (2010). Isolation of bacteria and fungi from personal and public cellphones: A case of Bayero University, Kano (old campus). International Journal of Biomedical and Health Sciences, 6(1): 97 - 102.
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