Potentials of Acremonium butyri fungus in pre-treatment and hydrolysis using Rice husk substrate for biofuel production: A short communication
DOI:
https://doi.org/10.47430/ujmr.2052.018Keywords:
Acremonium butyri, Rice Husk, Pre-treatment, Hydrolysis, BiofuelAbstract
There is increasing interest in the area of biofuel production due to fear of fossil fuel extinction as a result of over exploitation and crises. During biofuel production, substrate needs to undergo pre-treatment and hydrolysis where acids and alkali are mostly used. In this study, Acremonium butyri was used for both pre-treatment and hydrolysis. Structural compositions of the rice husk were determined. Extractives, hemicellulose and Lignin content was determined via extraction using Soxhlet extractor while cellulose was determined as the difference from the extractives, hemicelluloses and lignin. Acremonium butyri was isolated from dried roots of Piliostigma reticulatum (Kalgo) by keeping the roots in a clean plastic for a period of 7 days on moist environment after which fungal growth appeared. The growth was aseptically transferred on to prepared S.D.A plate and kept at room temperature. The fungal growth was identified as based on the physical and microscopic characteristics. About 50g of rice husk was mixed with 500 ml of distilled water in the ration of 1:10 to obtain homogenous slurry and then inoculated with 2ml of prepared Acremonium butyri solution and incubated at room temperature for up to
3 weeks with frequent shaking at certain intervals. Reducing sugar test was carried out to determine the reducing sugar released with UV-VIS spectrophotometer. The results obtained indicate that rice husk contained 32%, 30%, 29% and 8.4% of cellulose, hemicelluloses, extractives and lignin respectively. And a total of 0.936g/l of reducing sugar was released after 3 weeks of pre-treatment. The results implies that Acremonium butyri separated the component of rice husk (pre-treatment) as well as break down cellulose and hemicelluloses into its monomers (hydrolysis) thereby releasing sugar. Hence, Acremonium butyri is a good microorganism for biological pre-tretment and hydrolysis.
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References
Abdullahi, A. A., Aliero, B. L., Aliero, A. A., Zuru, A.A and Rabah, A. B. (2017). Optimization of Ethanol Production from Switchgrass (Panicum virgatum L.) Grown in Sokoto Geoecological Zone of Nigeria. International Journal of Chemistry, Pharmacy and Technology, 2(1), 43-56.
Adesina, F. C and Onilude A. A (2013). Isolation, identification and screening of xylanase and glucanase-producing microfungi from degrading wood in Nigeria. African Journal of Agricultural Research. 8 (34), pp. 4414-4421
https://doi.org/10.5897/AJAR2013.6993
Adila, M. M., Jeng, S. L., Nasir, S. A., Hashim,H. and Shin, W. H (2019) Characteristics of Cellulose, Hemicellulose and Lignin of MD2 Pineapple Biomass. Journal of Chemical Engineering Transactions, 72. 79-82
Anne, K. (2017). Development of pretreatment technology and enzymatic hydrolysis for biorefineries. Doctoral dissertation for the degree of Doctor of Science in Technology to be presented with due permission of the School of Chemical Technology for public examination and debate in Auditorium V1 in Material technology building, at Aalto University School of Chemical Technology. Unpublished.
Anwar, M., Bambang, P and Nita, A (2017). Lignin Isolation Process from Rice Husk by Alkaline Hydrogen Peroxide: Lignin and Silica Extracted. AIP Conference Proceedings 1823, 020013.
Anwar, Z., Gulfraz, M. and Irshad, M (2014) Agro-industrial Lignocellulosic biomass a Key to Unlock the Future Bio- energy: A Brief Review. Journal of Radiation Research and Applied Sciences 7(2), 163-173.
https://doi.org/10.1016/j.jrras.2014.02.003
Ayeni A. O., Hymore F. K., Mudliar S. N., Deskmukh S. C., Satpute D. B., Omoleye J. A. and Pandey R. A. (2013). Hydrogen peroxide and lime based oxidative pretreatment of wood waste to enhance enzymatic hydrolysis for a biorefinery: process parameters optimization using response surface methodology. Journal of Fuel, 106, 187-194.
https://doi.org/10.1016/j.fuel.2012.12.078
Ayeni, A. O., Adeeyo, O. A., Oyinlola, M. O. and Temitayo E. O (2015) Compositional Analysis of Lignocellulosic Materials: Evaluation of an Economically Viable Method Suitable for Woody and Non-Woody Biomass. American Journal of Engineering Research, 4(4). 14-19
Banerjee, S., Mudliar, S., Sen, R., Giri, B., Satpute, D., Chakrabarti, T., and Pandey, R.A. (2010). Commercializing Lignocellulosic Bioethanol: Technology Bottlenecks and Possible Remedies. Biofuels Bioproducts and Biorefining. 4, 77-93.
https://doi.org/10.1002/bbb.188
Ben-iwo, J., Manovic, V., and Longhurst, P. (2016). Biomass resources and biofuels potential for the production of transportation fuels in Nigeria. Renewable and Sustainable Energy Reviews, 63, 172-192. https://doi.org/10.1016/j.rser.2016.05050
https://doi.org/10.1016/j.rser.2016.05.050
Bisaria, V.S. (1991). Bioprocessing of Agro- residues to glucose and chemicals in Bioconversion of Waste Materials to Industrial Products. Martin, A.M. (Ed.), Elsevier, London, Pp. 210-213.
Blasi C. D., Signorelli G., Di Russo C., and Rea G. (1991). Product distribution from pyrolysis of wood and agricultural residues, Industrial Engineering Chemistry Research, 38(6), 2216-2224.
https://doi.org/10.1021/ie980711u
Bonelba, P. and Ferrara, M. (2007). Bioethanol via enzymatic Hydrolysis of Cellulosic Biomass. Accessed fromhttp://www.foa.org/biotech/seminaro ct2007.html
Duff, S. J. B. and Murray, W. D., (1996). Bioconversion of Forest Products Industry Waste Cellulosics to Fuel Ethanol: a review. Journal of Bioresource Technology. 55, 1-33.
https://doi.org/10.1016/0960-8524(95)00122-0
Ezeoha1, S. L., Anyanwu, C. N. and Nwakaire,J. N. (2017). The Prospects, Impacts, and Research Challenges of Enhanced Cellulosic Ethanol Production: A Review. Nigerian Journal of Technology 36(1), 267-275.
https://doi.org/10.4314/njt.v36i1.32
Ezenwanne, V. C. (2014). Fermentation Products of Rice Husks, by Bacteria and Fungi Isolates from Rice Husk Dump Site. A dissertation submitted to University of Nigeria Nsukka in partial fulfillment of the Requirements for the award of degree of master of Science (m.sc.) In industrial biochemistry. Pp 17-25. Not punlished
Iyang, E. J. (2014) Evaluation of Pre-treatmemt Methods for Enzymatic Saccarification of Alstonia boonei Wood Sawdust and Fermentation Using Saccharomyses cereviciae and Zymomonas mobilis for Ethyl Alcohol Production. Masters Dissertation Submitted to University of Nigeria Nsukka. Not Published.
Jie, D. M. S. (2014). Butanol Production from Lignocellulosic Biomass and Agriculture Residues by Acetone-Butanol-Ethanol Fermentation. A Dissertation Presented in Partial Fulfillment of the Requirements for the Degree, Doctor of Philosophy in the Graduate School of the Ohio State University. 210-255. Unpublished.
Khamaiseh, E. I., Hamid, A. A., Abdeshahian, P., Mohtar, W., Yusoff, W., and Kalil,M. S. (2014). Enhanced Butanol Production by Clostridium acetobutylicum NCIMB 13357 Grown on Date Fruit as Carbon Source in P2 Medium. The Scientific World Journa. Hindawi Publishing Corporation. http://dx.doi.org/10.1155/2014/39575 4
https://doi.org/10.1155/2014/395754
Li, S., Xu S., Liu S., Yang C and Lu Q (2004). Fast pyrolysis of biomass in free-fall reactor for hydrogen-rich gas. Journal of Fuel Process Technology, 85(8-10), 1201-1211.
https://doi.org/10.1016/j.fuproc.2003.11.043
Lin L., Yan R., Liu Y and Jiang W. (2010). In- depth investigation of enzymatic hydrolysis of biomass wastes based on three major components: cellulose, hemicellulose, and lignin, Bioresource Technology, 101 (21), 8217-8223.
https://doi.org/10.1016/j.biortech.2010.05.084
Lynd, L., Weimer, P., Van, W., Pretorius, l., (2001). Micobial Cellulose Utilization: Fundamentals and Biotechnology. Journal of Microbology and Moleculer Biology 66:506-577
https://doi.org/10.1128/MMBR.66.3.506-577.2002
Mahapatra, M. K., and Kumar, A. (2017). A Short Review on Biobutanol , a Second Generation Biofuel Production from Lignocellulosic Biomass, Journal of Clean Energy Technologies. 5(1), 27-30. https://doi.org/10.18178/jocet.2017.5. 1.338
https://doi.org/10.18178/JOCET.2017.5.1.338
Miller G. L (1959). Use of dinitrosalicylic acid reagent for determination of reducing. Journal of Analytical Chemistry. 31: 426
https://doi.org/10.1021/ac60147a030
Negi, S. and Banerjee, R. (2006). Optimization of Amylase and Protease Production from Aspergillus awamori in Single Bioreactor Through EVOP Factorial Design Technique. Journal of Food Technology and Biotechnology. 44 (2),257-261.
Neves, M. A., Kimura, T., Shimizu, N. and Nakajima, M. (2007). State of the art and future trends of bioethanol production. Dynamic Biochemistry, Process Biotechnology and Molecular Biology 1 (1), 1-14.
Noha, S. E. (2015). Utilization of Energy from Residual Biomass of Rice Husk in Egypt. A Doctoral Thesis Submitted to university of Granada. Unpublished.
Okano, K., Kitagawa, M., Sasaki, Y. and Watanabe, T. (2005). Conversion of Japanese Red Cedar (Cryptomeria japonica) into a Feed for Ruminants by White-Rot Basidiomycetes. Journal of Animal Feed Science Technology, 120: 235-243.
https://doi.org/10.1016/j.anifeedsci.2005.02.023
Oyeleke, S. B. and Manga, S. B. (2008). Essentials of Laboratory Practical in Microbiology. First Edition, Tolbest Publishers, Minna, Nigeria. 36-67.
Oyeleke, S. B., Shnada, H. A. and Egwim, E. (2010). Production and Characterization of Amylase produced by Bacillus megaterium Isolated from a Local Yam Peels Dumpsite in Minna, Niger State. Journal of Microbiology and Antimicrobials 2(7), 88-92.
Park, E.Y., Anah, P. N. and Okuda, N. (2004). Bioconversion of waste office paper to L(+)-lactic acid by the filamentous fungus Rhizopus oryzae, Journal of Bioresource Technology.
https://doi.org/10.1016/j.biortech.2003.08.017
Rabah, A. B., Oyeleke, S. B., Manga, S. B. and Hassan, L. G. (2011). Utilization of millet and guinea corn husks for Bioethanol production. African Journal Microbiological Resource; 5(31): 5721-5724.
https://doi.org/10.5897/AJMR11.1127
Rajesh, A., Rajesh, E., Rajendran, R., and Jeyachandrans, N., (2008). Production of Bioethanol from Cellulosic Cotton Waste through Microbial Extracellular Enzymatic Hydrolysis and Fermentation. Electronic Journal of Environment Agricultural and Food Chemistry. 7(6); 2984-2994.
Rowell, R. M. ( 2012) Handbook of wood chemistry and wood composites, CRC press, Boca Raton, Florida, USA.
https://doi.org/10.1201/b12487
Saha, B. C., Iten, L. B., Cotta, M. A. and Wu, Y. V. (2005). Dilute Acid Pretreatment, Enzymatic Saccharification and Fermentation of Wheat Straw to Ethanol. Journal of Process Biochemistry 40: 3693-3700.
https://doi.org/10.1016/j.procbio.2005.04.006
Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D. and Crocker, D. (2008). Determination of Structural Carbohydrates and Lignin in Biomass, Colorado, Laboratory. Analytical Procedure.
Sticlen, M. B. (2007). Feedstock Crop Genetic Engineering for Alcohol fuels. Journal of Crop Science. 47: 2238-2248.
https://doi.org/10.2135/cropsci2007.04.0212
Sun, Y., Cheng, J. (2002). Hydrolysis of Lignocellulosic Materials for Ethanol Production: A Review. Journal of Bioresouce and Technology 83, 1-11.
https://doi.org/10.1016/S0960-8524(01)00212-7
Sun, Y. and Jiayang, C. (2001). Hydrolysis of Lignocellulosic Materials for Ethanol production: A Review. Journal of Bioresource Technology 83 1-11
https://doi.org/10.1016/S0960-8524(01)00212-7
Teymouri, F., Laureano-Pérez, l., Alizadeh, H. and Dale, B. E. (2004). Ammonia Fiber Explosion Treatment of Corn Stover. Applied Biochemistry and Biotechnology 115(1-3): 951-963.
https://doi.org/10.1385/ABAB:115:1-3:0951
Wang, A., Wang, Y., Jiang, T., Li, L., Ma, C., and Xu, P. (2010). Production of 2,3- butanediol from corncob molasses, a waste by-product in xylitol production. Journal of Applied Microbiology and Biotechnology, 87(3): 965-970. DOI: 10.1007/s00253-010-2557-8
https://doi.org/10.1007/s00253-010-2557-8
Williams, P. T. and Nugranad, N. (2000). Comparison of products from the pyrolysis and catalytic pyrolysis of rice husks. Journal of Energy. 25:493-513.
https://doi.org/10.1016/S0360-5442(00)00009-8
Yang, B., Dai, Z. Y., Ding, S. Y., and Wyman, C.E. (2011). Enzymatic Hydrolysis of Cellulosic Biomass. Biofuels 2(4), 421- 450.
https://doi.org/10.4155/bfs.11.116
Yang, M. (2015). The use of lignocellulosic biomass for fermentative butanol production in biorefining processes. Dissertationes Forestales 202, 49. Available at:http://dx.doi.org/10.14214/df.202.
https://doi.org/10.14214/df.202
Zulkefflizan, J., Madihah, M. S. and Adibah, Y. (2017). Potential Use of Lignobiomass for Sugar Production. Journal of Applied Biotechnology and Bioengineering. 3 (6).
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