Optimization of Culture Condition in the Production of Bioenzymes by Bacteria Isolated from Poultry Waste in Sokoto State, Nigeria
DOI:
https://doi.org/10.47430/ujmr.1942.004Keywords:
poultry wastes, cellulase, Banana peels, Bacteria, EnzymesAbstract
Poultry wastes obtained from a poultry farm in Sokoto metropolis were analyzed for cellulose producing bacteria. Bacillus megaterium, Bacillus laterosporus, and Bacillus amyloliquifeciens isolated were screened for their ability to produce cellulase enzyme. All the isolates showed cellulose activity by exhibiting a wide halo on caboxymethylcellulase medium (CMC).The fermentation process was optimized using the following parameters, inoculum size, pH, Substrate concentration, temperature, and incubation periods. Cellulase activity was determined using DNS method, Banana peels was used as a substrate for the production of the enzymes, this was analysed with atomic absorbtion spectrophotometer (AAS).Cellulase enzyme was produced at innoculum size 1, 2, 3, 4, and 5%. pH 3, 5, 7, 9, and 11. Substrate concentration 1g, 2g, 3g, 4g, and 5g. Temperature 35, 45, 55, 65, and 75, for 1, 2, 3, 3, and 5, days respectively. Bacillus laterosporus recorded the highest cellulase activity 0f 0.37mg/ml in 5% substrate concentration among all the isolates while Bacillus amyloliquefeciens recorded highest cellulose production at pH3 with 45mg/ml Bacillus laterosporus recorded highest activity of cellulose production with 0.71mg/ml Temperature was also studied in the cellulose production and Bacillus laterosporus showed highest activity at 75⁰C with activity of 0.66mg/ml. This study showed that Bacillus laterosporus was the best cellulase producing bacteria among all the isolates.
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Bairagi A., Sarkar Ghosh K., Sen S.K., and Ray A.K. (2002a). Enzyme producing bacterial flora isolated from fish digestive tracts. Aquaculture International 10: 109-121.
https://doi.org/10.1023/A:1021355406412
Bakare, M. K., Adewale I.O. Ajayi A. and Shonukan O.O. (2005). Purification and Charachterization of Cellulase from the wild-type and two Improved Mutants of Pseudomonas fluorescence, African journal of biotechnology, Vol.4 (9) pp 898-904
Belghith, H., Ellouz-Chaabouni, S. and Gargouri, A. (2001). Stabilization of Penicillium occitanis Cellulases by Spray Drying in Presence of Maltodextrin. Enzyme and Microbial Technology, 28, 253-258.
https://doi.org/10.1016/S0141-0229(00)00322-7
Bertrand,T.F., Fredrie,T. and Robert, N.(2004). Production and partial characterisation of a thermostable amylase from Ascomycetes yeast strain isolated from starchy soil.Mc Graw Hill inc.New York.PP 57-59.
Brijwani, K. and Vadlani, P.V. (2011). Cellulolytic Enzymes Production via Solid State Fermentation: Effect of Pretreatment Methods on Physiochemical Characteristics of Substrate. Enzyme Research,Vol 86 :134.
https://doi.org/10.4061/2011/860134
Combe E., Dermarne Y., Gueguen L., Ivorec-Szylit O., Meslin J.H., and Sacquet E. (1976). Some aspects of the relationships between gastrointestinal flora and host nutrition. World Review of Nutrition and Dietetics 24: 1-57.
https://doi.org/10.1159/000399403
Coral , G. Arikan, B,. Unaldi M.N. and Guvenmez., H.(2002) .Thermostable alkaline protease produced by an Aspergillus niger strain. Journals of Microbiology.53:491-498.
Danulat E., and Kausch, H. (1984). Chitinase activity in the digestive tract of the Cod, Gadus morhua (L.). Journal of Fish Biology 24: 125-133.
https://doi.org/10.1111/j.1095-8649.1984.tb04784.x
Denison, D.A. and Koehn ,RD. (1977). Cellulase activity of pronia Oedipus. Mycologia, 69:592-603.
https://doi.org/10.1080/00275514.1977.12020097
Garcia-Martinez., D.V. Shimuyo A., Madia A., and Deman A.L., (1980). Studies on cellulase production by Clostridium Thermocellum.European Journal Applied Microbiology and Biotechnology 9: 189-197.
https://doi.org/10.1007/BF00504485
Gauam, S.P. Bundela, P, S. Pandey, A.K. Jamaluddin Khan, Awasthin M.K. and Sarsanya S. (2011).Optimization for the production of cellulose enzyme from municipal solid waste residue by two novel cellulolytic fungi. Biotechnology research international. Vol. 2:11 Article ID 810428.PP8
https://doi.org/10.4061/2011/810425
Goldin B.R. (1986). In situ bacterial metabolism and colon mutagens. Annual Review of Microbiology 40: 367-393.
https://doi.org/10.1146/annurev.mi.40.100186.002055
Goodrich.D., and Morita R.Y. (1977). Bacterial chitinase in the stomach of marine fishes from Yaquina Bay, Oregon, USA. Marine Biology 41: 355-360.
https://doi.org/10.1007/BF00389101
Haapela, R., Parkkinen, E., Susminen, P., Unk [1] Abe J, Bergman FW, Obata K, and Hikuri S (1988). Production of raw starch digesting amylase by Aspergillus K-27. Applied. Microbiology. Biotechnoogyl. 27(1): 447- 450.
https://doi.org/10.1007/BF00451611
Immanuel G., Dhanusha R., Prena P., and Palavesan A. (2006) Effect of different growth parameters on endoglucanase enzyme activity by bacteria isolated from coir retting effluents of estuarine environments. International Journal of environmental Science and Technology. 3: 25-34.
https://doi.org/10.1007/BF03325904
Jeffries, T.W. (1996). Production and Applications of Cellulase Laboratory Procedures, 1-10.
Kang S.W., Park Y.S., Lee J.S.,Hong SI and Kim S.W. Production of cellulases and Hemicellulases by Aspergillusn niger KK2 from lignocellulosic biomass. BioresourceTechnology Vol. 91920 pp153-156.
https://doi.org/10.1016/S0960-8524(03)00172-X
Kasyap, P., Sabu A., Pandey A, and Szakacs G. (2002) Extracellular leglutaminase production by Zygosaccharomyces rouxii under solid state fermentation. process Biochem 38; 307-312.
https://doi.org/10.1016/S0032-9592(02)00060-2
Kelly, C.T., M.A., Tigue., E.M., Doyle and Fogarty M.M.(1997). Raw Starch degrading alkaline amylase of Bacillus SP. Journal of Industrial Microbiology., 15 : 446-448.
https://doi.org/10.1007/BF01569973
Kono, M., Matsui, T., and Shimizu C. (1987). Chitin-decomposing bacteria in digestive tracts of cultured red sea bream and Japanese eel. Bulletin of the Japanese Society of Scientific Fisheries (Nippon Suisan Gakkaishi) 53: 305-310.
https://doi.org/10.2331/suisan.53.305
Lynd, L.R., Weimer P.J., Vanzyl W.H. and Pretorius I.S. (2002). Microbial cellulose utilization: mixed culture of Aspergillus niger and Saccharomyces cerevisiae grown on Sorghum. Microbial molecular. Bioogyl Review. 66(3): 506-577.
https://doi.org/10.1128/MMBR.66.3.506-577.2002
Mandels, M., Hontz, L. and Nyostrom, J. (1974). Enzymatic hydrolysis of Waste Cellulose Biotechnology Bioeng 16: 1471-1493.
https://doi.org/10.1002/bit.260161105
McBee R.H., Clarke R.T.J., and Bauchop T. (1977). Fermentation in the hindgut. (eds.) Microbial ecology of the gut. Academic Press, London. Pp.185-222
Minami, Z., Kajinta, M., and Hibino S. (1972). Studies on the utilization of petro-yeast as diet of fresh water fish. The distribution of chitin-decomposing bacteria in digestive tracts. Bulletin of Japanese Society of Scientific Fisheries 38: 1013-1019.
https://doi.org/10.2331/suisan.38.1013
Moriarty, D.J.W. (1990) Interactions of microorganisms and aquatic animals, particularly the nutritional role of the gut flora., In; Lesel R. (ed) Microbiology in poikilotherms. Elsevier, Amsterdam. pp 217-222.
Nochur S.V., Robertsm, F. and Deman A.l. True cellulose production by clostridium grown on different carbon source. Journal of bacteriology Vol 15 6 pp 641-646.
https://doi.org/10.1007/BF00138556
Pranner, J. (1979). Environmental Microbiology and Waste Utilization G//AMV Proceedings (ed emejuaiwe), Academic Press, London, 67-69.
Rajoka. (2004). Influence of various fermentation variables on exo-glucanase production in cellulomonas flavigena. Electronic Journal of Biotechnology 43:15 22.
https://doi.org/10.2225/vol7-issue3-fulltext-2
Ramachandran, S., Patel A.K., Nanpiothiri, K. M., Francis F., Nagy V., Szakac S.G., Pandey A. (2004). Coconut oil cake a potential raw material for the production of amylase. Bioresource Technol 93;169-174.
https://doi.org/10.1016/j.biortech.2003.10.021
Ray, A.K., Bairagi A, Sarkar Ghosh k, and Sen S.K. (2007). Optimization of fermentation conditions for cellulose production by Bacillus subtilis CY5 and Bacillus circulans TP3. Isolated from Fish Gut, Acta ichthyologicaet piscatorial, Vol 37 (1) pp 47-53.
https://doi.org/10.3750/AIP2007.37.1.07
Saha A.K., and Ray A.K. (1998). Cellulase activity in rohu fingerlings. Aquaculture International 6: 281-291.
https://doi.org/10.1023/A:1009210929594
Saxena S., Bahadur J.,and Varma A. (1993). Cellulose and hemi-cellulose degrading bacteria from termite gut and mound soils of India. International Journal of Microbiology 33: 55-60.
Stewart J.C,and Parry J.B. (1981). Factors influencing the production of cellulase by Aspergillus fumigates (Fresenius). Journal of General Microbiology 125: 33-39.
https://doi.org/10.1099/00221287-125-1-33
Stickney R R., and Shunway S.E. (1974). Occurance of cellulose activity in the stomachs of fish, Journal of fish Biology 6:779-790.
https://doi.org/10.1111/j.1095-8649.1974.tb05120.x
Sugita H., Miyajima C., and Deguchi Y. (1991). The vitamin B12-producing ability of the intestinal microflora of freshwater fish. Aquaculture 92: 267-276.
https://doi.org/10.1016/0044-8486(91)90028-6
Trust T.J., Bull L.M., Currie B.R., and Buckley J.T. (1979). Obligate anaerobic bacteria in the gastrointestinal microflora of the grass carp. (Ctenopharyngodon idella), goldfish. (Carassius auratus), and rainbow trout (Salmo gairdneri). Journal of Fisheries Research Board of Canada 36:1174-1179.
https://doi.org/10.1139/f79-169
Wen, Z., Liao, W. and Chen, S. (2005). Production of Cellulase/β-Glucosidase by the Mixed Fungi Culture Trichoderma reesei and Aspergillus phoenicis on Dairy Manure. Process Biochemistry, 40, 3087-3094.
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