Evaluation of Aflatoxin-Producing Fungi in Indoor Air of Warehouses and Houses of Farmers in Giwa, Kaduna State Nigeria
DOI:
https://doi.org/10.47430/ujmr.2272.012Keywords:
Aflatoxin-producing Fungi, Maize, Groundnut, Warehouse, Indoor Air, Aflatoxin-biosynthesis genesAbstract
The health risks associated with ingesting food contaminated with mycotoxins, particularly aflatoxin-contaminated staple foods like maize and other cereals, have been widely studied. However, there is little knowledge about the role of inhalation of pathogenic fungi as bioaerosols in contaminated air from handling crops as an occupational health risk. This paper presents a study aimed at determining the level of airborne aflatoxin-producing fungi in the indoor air of grain stores in the Giwa community of Kaduna State. Indoor air was sampled using the settling plate technique from grain stores, warehouses and living rooms. Metrological data of the studied area were collected from the Institute of Agricultural Research, ABU, Zaria. Airborne mycofloral concentrations were determined, and colonies of Aspergillus flavus were identified. The isolates were screened for aflatoxin production on Neutral Red Desiccated Coconut Agar (NRDCA). Selected aflatoxin-producing fungal isolates were screened for the presence of aflD (nor-1), aflM (ver-1) and AflR genes by PCR. Sampling was done once every month from October to December 2020. Mycofloral concentrations were in the range of 2.77x103−4.05x103 and 1.55x103−2.17x103CFUm-3for grain stores and living rooms respectively. A total of twelve (12) strains of A. flavus were isolated from the indoor air of the grain stores and warehouses while none was obtained from the living room. Eleven (11) isolates were confirmed to be aflatoxigenic on NRDCA, presenting 30 CFUm-3 of the indoor air mycofloral composition. The aflD, aflM and aflR were amplified with aflD being the most detected gene from all the aflatoxin-producing mould isolates of Aspergillus species. The mycofloral concentrations in the grain stores were higher than those in the living room and, in all the sampling sites, exceeded the limit of the total mycofloral concentration of 500 CFUm-3 for agricultural and industrial environments. There were significant differences (p<0.05) in the indoor mycofloral concentrations between the grain stores/warehouses and the living room. The presence of aflatoxigenic strains of A. flavus in the stores indicates that grain handlers and traders are at risk of occupational exposure to aflatoxigenic fungi and aflatoxins. Hence, they should wear protective materials for their safety while working in such stores.
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References
American Conference of Governmental Industrial Hygeinists (ACGIH) (2011). Threshold Limit Values for Chemical Substances, Physical Agents and Biological Exposure Indices. Cincinnati, OH. ISBN:9781607260196.
Adhikari, A., Reponen, T., Lee, S. A., Grinshpun, S.A., (2004). Assessment of human exposure to airborne fungi in agricultural confinements: personal inhalable sampling versus stationary sampling. Annals of Agricultural and Environmental Medicine, 11:269- 277.
Agriopoulou, S., Stamatelopoulou, E. and Varzakas, T. (2020). Advances in Occurrence, Importance and mycotoxin Control Strateggies: Prevention and Detoxification in Foods. Foods, 9(137):1- 48. https://doi.org/10.3390/foods9020137
Aleksic, B., Draghi, M., Ritoux, S., Bailly, S., Lacroix, M., Oswald, I. P., Bailly, J. D. and Robine, E. (2017) Aerosolization of mycotoxins after growth of toxinogenic fungi on wallpaper. Applied Environmental Microbiology,83:e01001- 17. https://doi.org/10.1128/AEM.01001-17
Alkadri, D., Rubert, J., Prodi, A., Pisi, A., Manes, J. and Soler, C. (2014). Natural co-occurrence of mycotoxins in wheat grains from Italy and Syria. Food Chemistry, 157:111-118. https://doi.org/10.1016/j.foodchem.2014.01.052
Amuzie, C. J., Harkema, J. R. and Pestka, J. J. (2008). Tissue distribution and proinflammatory cytokine induction by the trichothecene deoxynivalenol in the mouse: Comparison of nasal versus Oral exposure. Toxicology, 248:39-44. https://doi.org/10.1016/j.tox.2008.03.005
Atanda, O. O., Ogunrinu1, M. C. and Olorunfemi, F. M. (2011).A neutral red desiccated coconut agar for rapid detection of aflatoxigenic fungi and visual determination of aflatoxins. World Mycotoxin Journal, 4(2):147- 155. https://doi.org/10.3920/WMJ2010.1241
Anaya, M., Gamez-Espinosa, E., Falco A. S. et al., (2019). Characterization of indoor Air mycobiota of two locals in a food industry, Cuba. Air Quality, Atmosphere and Health, 12(7):797- 805. https://doi.org/10.1007/s11869-019-00707-7
Atanda1, O. O., Ogunrinu1, M. C. and Olorunfemi, F. M. (2011).A neutral red desiccated coconut agar for rapid detection of aflatoxigenic fungi and visual determination of aflatoxins. World Mycotoxin Journal, 4(2):147-155. https://doi.org/10.3920/WMJ2010.1241
Awad, A. H. and Mawla, H. A. (2012). Sedimentation with the Omeliansky formula as an accepted technique for quantifying airborne fungi. PolicyJournal of EnvironmentalStudies,21:1539-1541.
Benkerroum, N. (2020). Chronic and Acute Toxicities of Aflatoxins: Mechanisms of Action. International Journal of Environmental Research Public Health. 17:423- 463. https://doi.org/10.3390/ijerph17020423
Bennett, J. W. and Klich, M. (2003) Mycotoxins. Clinical Microbiology Review; 16: 497-516. https://doi.org/10.1128/CMR.16.3.497-516.2003
Criseo, G., Bagnara, A. and Bisignano, G. (2001). Differentiation of aflatoxin-producing and non-producing strain of Aspergillus flavus group. Journal of Applied Microbiology, 33: 291 − 295. https://doi.org/10.1046/j.1472-765X.2001.00998.x
Davari, E., Mohsenzadeh, M., Mohammadi, Gh.and Rezaeian-Doloei, R. (2015). Characterization of aflatoxigenic Aspergillus flavus and A. parasiticus strain isolates from animal feedstuffs in northeastern Iran. Iranian Journal of Veterinary Research,2(16):150- 155.
Degen, G. H. (2011) Tools for investigating workplace-related risks from mycotoxin exposure. World Mycotoxin Journal,4:315-27. https://doi.org/10.3920/WMJ2011.1295
Dooso, O. R., Okoth S., Wachira, P. and Mutiga, S. (2019).Genetic Profiling of Aspergillus Isolates with Varying Aflatoxin Production Potential from Different Maize-Growing Regions of Kenya. Toxins,11(467):1- 19. https://doi.org/10.3390/toxins11080467
Halstensen, A. S. (2008) Species-specific Fungal DNA in Airborne Dust as Surrogate forOccupational Mycotoxin Exposure?International Journal ofMolecular Sciences9:2543- 2555. https://doi.org/10.3390/ijms9122543
Hocking, D. A. (2007). Food Microbiology: Fundermentals and Frontiers, 3rd Edition. ASM Press, Washington, D. C. pp. 25.
Hussain A., Afzal, A., Muhammad, I. and Kausar A. M. (2015) Molecular Detection of aflatoxin producing strains of aspergillus flavus from peanut. Turkish journal of agriculture- Food Science and Technology, 3(5):335- 341. https://doi.org/10.24925/turjaf.v3i5.335-341.123
Lanier, C., Richard, E., Heutte, N. etal. (2010). Airborne molds and mycotoxins associated with handling of corn silage and oilseed cakes in agricultural environment Elsevier; Atmospheric Environment,44:1980- 1986. https://doi.org/10.1016/j.atmosenv.2010.02.040
Marin, S., Ramos, A. J., Cano-Sancho, G. et al. (2013) Mycotoxins: occurrence, toxicology, and exposure assessment. FoodChemistry and Toxicology,60:218-237. https://doi.org/10.1016/j.fct.2013.07.047
Mayer, S., Twarużek, M., Błajet-Kosicka, A. and Grajewski, J. (2016).Occupational exposure to mould and microbial metabolites during onion sortinginsights into an overlooked workplace. Environment Monitoring Assessement,188(154):1- 10. https://doi.org/10.1007/s10661-016-5150-5
Mayer, S. (2016). Occupational Exposure to Mycotoxin and Preventive Measures. Environmental mycotoxin in public Health. Pp 325 - 341. https://doi.org/10.1016/B978-0-12-411471-5.00019-3
QIAgen (2022). DNA mini kit Handbook, Available at www.qiagen.com/ch/search/products accessed on 20th March 2022.
Shittu, A. I., Njoku, L. k. and Adesuyi, A. A. (2019). Indoor air quality and Microbiological Assessment of the Nigerian University Campus in Lagos. Ecological Safety and Balanced used of Resources,1(19):94- 103. https://doi.org/10.31471/2415-3184-2019-1(19)-94-103
Udomkun, P., Wiredu, A. N., Nagle, M., Muller, J., Vanlauwe, B. and Bandyopadhyay, R. (2017). Innovative Technologies to Manage Aflatoxins in Foods and Feeds, and the Profitability of Application. Food Control,76:127-138. https://doi.org/10.1016/j.foodcont.2017.01.008
Veigas, S., Veigas, C. and Oppliger, A. (2018). Occupational Exposure to Mycotoxin;Current Knowledge and Prospects. Annals of Work Exposure and Health, xx(xx):1-19.
Volenik, M., Rozman, V., Kalinovic, I. et al. (2007). Influence of Relative Humidity and Temperature on the Changes in Grain Moisture, in Stored Soybean and Maize. Agriculturae Conspectus Scientificus, 72(2):215- 219.
Yu, J., Chang, P. K., Kenneth, C. E. et al., (2004). Clustered Pathway Genes in Aflatoxin Biosynthesis. Applied and Environmental Microbiology,3(70):1253 - 1262. https://doi.org/10.1128/AEM.70.3.1253-1262.2004
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