Disparity of Biofilm Formation in Salmonella typhimurium on Glass and Wood Surfaces at Diverse Incubation Temperatures

Abstract

Salmonella Typhimurium is a pathogenic gram-negative bacterium that commonly causes food- borne diseases known as gastroenteritis. Biofilm can exist on many surfaces in food preparation

e.g glass, wood, plastic, metal and other food items. The aim of this study was to evaluate and quantify the biofilm formation of S. typhimurium on food contact surfaces (glass and wood) at different intervals (24, 48 and 72 hours) and different incubation temperatures (10, 28 and 37 oC). The inoculum used was 10⁸ CFU ml^-1 of S. typhimurium. Formation of biofilm was quantified using beads vortex and agar plate count method. Biofilm of S. typhimurium was formed on both surfaces with preference more to glass surfaces, but all the densities of biofilm formed can cause infections. On Glass surfaces a total of 1.62x10⁹ CFU ml^-1 S. typhimurium was achieved as the highest biofilm density. Thirty seven degree was the most preferred incubation temperature of S. typhimurium cells, through which the highest biofilm cells were formed and 10^oC incubation temperature supported the least biofilm densities. The result showed that, the biofilm formation of S. typhimurium was influenced by incubation temperatures and increases with time.