Antimicrobial Activity of Acacia Nilotica and Ziziphus Mauritiana against Clinical Isolates of Escherichia Coli and Klebsiella Aerogenes

Usman, A.; Ahmad, M.; Hamza, M. M.; Hussaini I. M.; Sanusi, S. B.; Innocent, A. A

doi:10.47430/ujmr.2382.001

Authors

Usman, A. Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna. https://orcid.org/0009-0005-8238-3651
Ahmad, M. Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna
Hamza, M. M. Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna
Hussaini I. M. Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria
Sanusi, S. B. Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna
Innocent, A. A Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna.

DOI:

https://doi.org/10.47430/ujmr.2382.001

Keywords:

Klebsiella aerogenes, Escherichia coli, Acacia nilotica, Ziziphus mauritania, antibacterial activity

Abstract

Klebsiella aerogenes is an opportunistic pathogen linked to several nosocomial illnesses, including pneumonia, sepsis, and infection of the urinary tract. Escherichia coli is frequently the source of numerous common bacterial illnesses, including bacteraemia, cholecystitis, cholangitis, urinary tract infections (UTI), traveler's diarrhoea, and other medical conditions such as meningitis in babies and pneumonia. This study aimed at determining the antimicrobial activity of Acacia nilotica and Ziziphus mauritania on clinical isolate of E. coli and K. aerogenes. The phytochemical constituents of Z. mauritiana and A. nilotica were determined. E. coli and K. aerogenes isolates were obtained from the Barau Dikko Teaching Hospital, Kaduna and reconfirmed using standard microbiological techniques. The antibacterial activity of Z. mauritiana and A. nilotica extracts against the isolates was determined using Agar well diffusion assay, the MIC and MBC was also determined and recorded. The findings of this study revealed that tannins, quinone, phenols, terpenoids, and steroids are present in Z. mauritiana extract while tannins, saponin, quinones, and terpenoids in A. nilotica extract. There was no activity of Z. mauritiana against any of the test isolates at all concentrations used in this study. Whereas A. nilotica exhibited an antibacterial activity against both E. coli and K. aerogenes recording respectively a zone of inhibition of 24 mm and 25 mm with MIC and MBC value of 600 mg/ml. This research displayed an antibacterial activity of A. nilotica and no activity of Z. mauritiana against E. coli and K. aerogenes.

Downloads

Download data is not yet available.

References

Abd'quadri-Abojukoro, A. N., Nkadimeng, S. M., McGaw, L. J. and Nsahlai, I. V. (2022). Phytochemical composition and cytotoxicity of ethanolic extracts of some selected plants. Journal of Applied Animal Research, 50 (1): 656-665. https://doi.org/10.1080/09712119.2022.2119979

Abeer, M., Ali, H. and Sanaa, O.Y. (2007). Antibacterial activity of Acacia nilotica extracts against some bacterial isolated from specimen. Research journal of medicinal plants,1(1): 25-28. https://doi.org/10.3923/rjmp.2007.25.28

Adeeyo, A., Odiyo, O.J. and Odelade, K. (2018). Chemical profiling and antimicrobial properties of phyto-active extracts from Terminalia glaucescens stem against water microbial contaminants. The Open Biotechnology Journal, 12(1). https://doi.org/10.2174/1874070701812010001

Alduraihem, N. S., Bhat, R. S., Al-Zahrani, S. A., Elnagar, D. M., Alobaid, H. M., and Daghestani, M. H. (2023). Anticancer and antimicrobial activity of silver nanoparticles synthesized from pods of Acacia nilotica. Processes, 11(2): 301. https://doi.org/10.3390/pr11020301

Ali, G., Farshad, A., Azizollah Y. and Taghavi, M.S. (2012). Nasim Behnoud Medicinal and Nutritional Properties of Ziziphus jujuba Mill. in Traditional Persian Medicine and Modern Phytotherapy Crescent. Journal of Medical and Biological Sciences, 6(2): 146-150

Alsayari, A. and Wahab, S. (2021). Genus ziziphus for the treatment of chronic inflammatory diseases. Saudi Journal of Biological Sciences, 28(12): 6897-6914. https://doi.org/10.1016/j.sjbs.2021.07.076

Andrew, J. M. (2001). Determination of Minimum Inhibitory Concentrations. Journal of Antimicrobial Chemotherapy, 48(1): 5-16. https://doi.org/10.1093/jac/48.suppl_1.5

Cheesbrough, M. (2012). District Laboratory Practice in Tropical Countries Part 2. Cambridge University Press, USA. 47-57.

CLSI (2012). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Approved Standard, 9th ed., CLSI document M07-A9. Clinical and Laboratory Standards Institute, 950.

Cubero, R. E., Carvajal‐Miranda, Y., Rodríguez, G., Álvarez‐Valverde, V., & Jiménez‐Bonilla, P. (2023). Antioxidant and antimicrobial activity of two Costa Rican cultivars of Ber (ziziphus mauritiana): An underexploited crop in the American tropic. Food Science and Nutrition. https://doi.org/10.1002/fsn3.3317

Daoudi, Y., Daoudi, S., Bennacer, S. S., Mohamed, K., Michael, H., Bouras, N. and Sabaou, N. (2017). Effect of culture media on the agar diffusion method for testing antibacterial activity against Escherichia coli (HO2). Journal of research science, (10): 1112-7163

Dhama, K.., Tiwari, R., and Chakrabort S. (2014). Evidence based antibacterial potentials of medicinal plants and herbs countering bacterial pathogens especially in the era of emerging drug resistance: an integrated update. International Journal of Pharmacology, 10(1): 1-4. https://doi.org/10.3923/ijp.2014.1.43

Farzana, M., Tharique, I.A. and Sultana, A. (2014). A review if ethnomedicine, phytochemical and pharmacological activities of Acacia nilotica. Journal of pharmacology and phytochemistry, 3: 84-90.

Garg, R., Anand, N., & Kumar, D. (2016). Pyrolysis of Babool seeds (acacia nilotica) in a fixed bed reactor and bio-oil characterization. Renewable Energy, 96: 167-171. https://doi.org/10.1016/j.renene.2016.04.059

Gu, H., Cai, Q., Dai, X., Wang, H., Xu, W., Cao, X., and Ye, Y. (2022). A case report of Klebsiella aerogenes-caused lumbar spine infection identified by metagenome next-generation sequencing. BMC Infectious Diseases, 22(1). https://doi.org/10.1186/s12879-022-07583-0

Haris, N.G., Taura, D.W, Abubakar, A.I., Yahaya, S.M., Amed, I., Aisha, S.B, (2017). Phytochemical Screening and Antibacterial Activity of Mentha piperita leaves extract on Uropathogenic Escherichia coli. Ewemen Journal of Microbial Research, 4(1): 51-58.

Hussain, S., Butt, S. Z., & Khurram Shahzad Munawar. (2021). Phytochemistry of ziziphus mauritiana; its nutritional and pharmaceutical potential. Scientific Inquiry and Review, 5(2). https://doi.org/10.32350/sir.52.01

Khamsatul, M. L., Fatimatul, M., Taslim, E., Mardi, S., Eko, S., Yunin, H., and Irsad, R. (2019). Antibacterial Activity of Leaves Extract of Bukkol (Ziziphus mauritania Lam) against E. coli and S. aureus. International Conference on Basic Sciences and Its Applications, 182.

Magnini, R. D., Hilou, A., Millogo-Koné, H., Pagès, J. M. and Davin-Regli, A. (2020). Acacia senegal extract rejuvenates the activity of Phenicols on selected Enterobacteriaceae multi drug resistant strains. Antibiotics, 9(6): 323. https://doi.org/10.3390/antibiotics9060323

Mengesha Y. A. (2015). Phytochemical extraction and screening of bio active compounds from black cumin (Nigella sativa) seeds extract. American Journal of Life Sciences, 3(5): 358. https://doi.org/10.11648/j.ajls.20150305.14

Nuria, M., Faizatun, A. and Sumantri (2009). Uji aktivitas antibakteri ekstrak etanol daun jarakpagar (Jattopha curcas L) terhadap bakteri Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan Salmonella typhi ATCC 1408, Mediagro, 26:2,26-37.

Omololu A. J., Omololu, O. O., Adekanye, N., Owolabi, T. A. and Shesha, A. (2017). Antimicrobial susceptibility pattern of escherichia coli isolates from clinical sources at tertiary health care setting, Ile Ife, Southwestern Nigeria. European Journal of Experimental Biology, 07(01). https://doi.org/10.21767/2248-9215.100005

Parveen, S., Bukhari, N., Nazir, M., Qureshi, W. A., Yaqoob, A., and Shahid, M. (2023). Phytochemical analysis, in-vitro biological activities and Pearson correlation of total polyphenolic content with antioxidant activities of Ziziphus mauritiana fruit pulp and seed during different ripening stages. South African Journal of Botany, 157: 346-354. https://doi.org/10.1016/j.sajb.2023.03.064

Saroinsong, M. S., Kandou, E. F., Papu, A. and Singkoh, F. O. (2014). Uji Daya HambatEkstrak Metanol Beberapa Jenis Porifera Terhadap Bakteri Escherichia coli and Staphylococcus aureus, 3:129-133. https://doi.org/10.35799/jm.3.2.2014.5989

Shukla, R. S., & Sharma, V. (2023). Validating the antimicrobial potentiality of peptides from pods of acacia nilotica willd.ex delile: A spotlight on bacterial fauna. Toxicology International: 111-119. https://doi.org/10.18311/ti/2023/v30i1/31892

Srivastava, M., Kumar, G., Mohan, R. and Malhotra, S. (2014). Phytochemical studies and antimicrobial activity of babool seeds. Journal of Scientific and Industrial Research., 73: 724-728

Wesevich, A., Sutton, G., Ruffin, F., Park, L. P., Fouts, D. E., Fowler, V. G., and Thaden, J. T. (2020). Newly named Klebsiella Aerogenes (formerly Enterobacter aerogenes) is associated with poor clinical outcomes relative to other enterobacter species in patients with bloodstream infection. Journal of Clinical Microbiology, 58(9). https://doi.org/10.1128/JCM.00582-20

Yusof, S. A. M., & Saat, R. (2017). Phytochemical Analysis and Bioactivity Studies of Ziziphus Mauritiana (Twigs and Leaves). Journal of Academia UiTM Negeri Sembilan, 5: 17-26.

Antimicrobial Activity of Acacia Nilotica and Ziziphus Mauritiana against Clinical Isolates of Escherichia Coli and Klebsiella Aerogenes

Authors

DOI:

Keywords:

Abstract

Downloads

References

Downloads

Published

How to Cite

Issue

Section

License

Most read articles by the same author(s)

Make a Submission