Antibacterial Efficacy of Citrullus lanatus (Watermelon) Seeds Extracts against Wound Infecting Staphyloccocus aureus and Pseudomonas Aeruginosa

Authors

  • Musa, B. Department of Microbiology, Kaduna State University, Kaduna, Nigeria
  • Shimfuo, B. Y. Department of Microbiology, Kaduna State University, Kaduna, Nigeria
  • Muhammad, J. Department of Microbiology, Kaduna State University, Kaduna, Nigeria
  • Bako, G. D. Department of Microbiology, Kaduna State University, Kaduna, Nigeria
  • Sanusi, S. B. Department of Microbiology, Kaduna State University, Kaduna, Nigeria

DOI:

https://doi.org/10.47430/ujmr.25103.019

Keywords:

Phytochemical, Antibacterial, Citrullus lanatus seeds, Staphylococcus aureus, Pseudomonas aeruginosa

Abstract

Watermelon seeds, originally from vine plants, are rich in nutrients and bioactive compounds.  This study aimed to assess the antibacterial efficacy of Citrullus lanatus (Watermelon) seed extracts against wound-infecting Staphylococcus aureus and Pseudomonas aeruginosa.  Preliminary phytochemical screening of the ethanol and aqueous extracts of the watermelon seed was carried out using standard analytical methods.  The two extracts were screened for antibacterial activity against S. aureus and P. aeruginosa, isolated from wound swabs, using both agar well diffusion and broth dilution assays.  The result of the phytochemical screening revealed the presence of saponins, steroids, tannins, phenols, quinones, and terpenoids in the ethanol extract, while saponins, steroids, quinones, phenols, terpenoids, and tannins were present in the aqueous extract.  The inhibitory zone of the ethanol extract against S. aureus ranged between 12.25±3.18 mm to 15.50±0.71 mm, while that of P. aeruginosa ranged between 12.80±0.00 mm to 15.25±1.06 mm at 50 and 100 (mg/mL), respectively.  The inhibitory zone of the aqueous extract against the clinical isolate of S. aureus ranged between 11.50±0.71 mm to 11.75±2.87 mm at 50 and 100 (mg/mL), respectively, while P.  aeruginosa exhibited inhibitory activity of 14.40±0.00 mm at a concentration of 100 mg/mL only.  The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC), on average, were 50 mg/mL and 100 mg/mL, respectively.  The observed inhibitory activity of the ethanol and aqueous extracts of the seed against the clinical isolates could be due to the presence of phytochemical components within the seed.

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References

Adebayo, A. H., Ibrahim, M. A., & Ibrahim, H. (2017). Phytochemical screening and antioxidant activity of Citrullus lanatus seed extract. Journal of Pharmacy and Pharmacology, 69(8), 1083–1092.

Adunola, A. T., Chidimma, A. L., Olatunde, D. S., & Peter, O. A. (2015). Antibacterial activity of watermelon (Citrullus lanatus) seed against selected microorganisms. African Journal of Biotechnology, 14, 1224–1229. https://doi.org/10.5897/AJB2014.14101

Alemu, F., Tilahun, A., & Ellas, E. (2017). In vitro antimicrobial activity screening of Punica granatum extracts against some human pathogens. Molecular Medicine: Current Aspects, 1, 1–10.

Athanasiadis, V., Chatzimitakos, T., Kalompatsios, D., Kotsou, K., Mantiniotou, M., & Lalas, S. I. (2023). Recent advances in the antibacterial activities of Citrullus lanatus (watermelon) by-products. Applied Sciences, 13(19), 11063. https://doi.org/10.3390/app131911063

Athar, A., Ghazi, A., Chourasiya, O., & Karadbhajne, V. Y. (2020). Watermelon seed oil extraction, analytical studies, modification and utilization of cosmetic industries. International Research Journal of Engineering and Technology (IRJET), 7(2), 1–15.

Baker, T. P., Corwin, B., & Jeft, L. W. (2012). Watermelon bacterial fruit biotechnology. European Journal of Medical Plant, 1(4), 171–179.

Bello, H. S., Ismail, H. Y., Goje, M. H., & Manga, H. K. (2016). Antimicrobial activity of Citrullus lanatus (Watermelon) seeds on some selected bacteria. Journal of Biotechnology Research, 2(6), 39–43.

Benmeziane, F., Arkoub, L., Hassan, K. A., & Zeghad, H. (2023). Evaluation of antibacterial activity of aqueous extract and essential oil from garlic against some pathogenic bacteria. International Food Research Journal, 25(2), 561–564.

Bereksi, M. S., Hussaine, H., Bekhchi, C., & Abdelouahid, D. E. (2018). Evaluation of antibacterial activity of some medicinal plants extracts commonly used in Algerian traditional medicine against some pathogenic bacteria. Pharmacognosy Journal, 10(3). https://doi.org/10.5530/pj.2018.3.83

Braide, W., Oddiong, I. J., & Oranusi, S. (2012). Phytochemical and antibacterial properties of the seed of watermelon (Citrullus lanatus). Prime Journal of Microbiology Research, 2(3), 99–104.

Covington, B. C., Xu, F., & Seyedsayamdost, M. R. (2021). A natural product chemist’s guide to unlocking silent biosynthetic gene clusters. Annual Review of Biochemistry, 90, 763–788. https://doi.org/10.1146/annurev-biochem-081420-102432

Dewu, M. M., Aminu, M., Suleiman, A. B., Umar, U. A., & Ismail, M. (2023). Antibacterial activity of Citrullus lanatus seed extract against clinical isolates of Klebsiella pneumoniae and Staphylococcus aureus. Dutse Journal of Pure and Applied Sciences, 9(3b). https://doi.org/10.4314/dujopas.v9i3b.21

Guerrero-Alvarez, G. E., & Giraldo-Rivera, A. I. (2023). Antibacterial activity of seed extracts of various species of the family Annonaceae cultivated in Colombia. Revista Colombiana de Ciencias Hortícolas, 17(1), 1–10. https://doi.org/10.17584/rcch.2023v17i1.15689

Gupta, A., Singh, A., & Prasad, R. (2018). A review on watermelon (Citrullus lanatus) medicinal seeds. Journal of Pharmacognosy and Phytochemistry, 7(3), 2222–2225.

Hassan, L. E. A., Sirat, H. M., Yagi, S. M. A., Koko, W. S., & Abdelwahab, S. I. (2011). In vitro antimicrobial activities of chloroformic, hexane and ethanolic extracts of Citrullus lanatus var. citroides (Watermelon). Journal of Medicinal Plant Research, 5, 1338–1344.

Hlaing, S. S., Oo, T., & Win, K. K. (2020). Spore tetrad and pollen fertility of three cultivars of Citrullus lanatus (Thunb) Mastum & Nakai. *3rd Myanmar Korea Conference Research Journal, 3*(3), 815–821.

Ibrahim, M. A., Adebayo, A. H., & Ibrahim, H. (2017). Phytochemical analysis and antimicrobial activity of Citrullus lanatus seed extract. Journal of Food Science and Technology, 54(4), 1056–1065.

Mogana, R., Adhikari, M. N., Tzar, R., & Wiart, C. (2020). Antibacterial activities of the extracts, fractions and isolated compounds from Canarium patentinervium Miq. against bacterial clinical isolates. BMC Complementary Medicine and Therapies, 20(55), 1–11. https://doi.org/10.1186/s12906-020-2837-5

Nwankwo, I. U., Onwuakor, C. E., & Nwosu, V. C. (2024). Phytochemicals analysis and antibacterial activities of Citrullus lanatus seed against some pathogenic microorganisms. Global Journal of Medical Research, 14, 17–22.

Oyeleke, S. B., & Manga, S. B. (2008). Essentials of laboratory practical in microbiology. Tobest Publishers.

Patra, J. K., Das, S. K., & Thatol, H. (2020). Isolation, culture and biochemical characterization of microbes. In A practical guide to environmental biotechnology (pp. 83–133). Springer. https://doi.org/10.1007/978-981-15-6252-5_4

Raji-Idowu, F. O. O. (2023). Antibacterial activities of fenugreek oil and seed extracts on selected pathogenic bacteria and proximate composition of fenugreek seed. Nigerian Journal of Microbiology, 37(2), 6729–6735.

Recent advances in the antibacterial activities of Citrullus lanatus (watermelon) by-products. (2023). Applied Sciences. Retrieved from MDPI.

Roy, B., Das, S., & Bhattacharyya, S. (2023). Overview on old and new biochemical test for bacterial identification. Journal of Surgical Case Reports and Images, 6(20), 1–11. https://doi.org/10.31579/2690-1897/163

Saquib, S. A., AlQahtani, N. A., Ahmad, I., Kader, M. A., Al Shahrani, S. S., & Asiri, E. A. (2019). Evaluation and comparison of antibacterial efficacy of herbal extracts in combination with antibiotics on periodontal pathobionts: An in vitro microbiological study. Antibiotics, 8(3), 89. https://doi.org/10.3390/antibiotics8030089

Sharma, R., & Kaur, S. (2016). Antibacterial and phytochemical screening of trikuta—traditional food of western Rajasthan. Indian Journal of Traditional Knowledge, 5(1), 90–93.

Tettey, C. O., Neglo, D., Essuman, E. K., Kortei, N. K., & Boakye, A. A. (2021). Comparative antioxidant and antimicrobial activities of the peel, rind, pulp and seeds of watermelon. Scientific African, 11(5), 582–597. https://doi.org/10.1016/j.sciaf.2020.e00582

Tiwari, P., Kumar, B., Kaur, G., & Kaur, H. (2011). Phytochemical screening and extraction: A review. International Pharmaceutical Science, 1, 98–106.

Published

30-06-2025

How to Cite

Musa, B., Shimfuo, B. Y., Muhammad, J., Bako, G. D., & Sanusi, S. B. (2025). Antibacterial Efficacy of Citrullus lanatus (Watermelon) Seeds Extracts against Wound Infecting Staphyloccocus aureus and Pseudomonas Aeruginosa. UMYU Journal of Microbiology Research (UJMR), 10(3), 176–184. https://doi.org/10.47430/ujmr.25103.019

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