In vitro Antimicrobial Activity of Some Plant Essential Oils against Organisms Associated with Urinary Tract Infection and Roasted Meat
DOI:
https://doi.org/10.47430/ujmr.1611.005Keywords:
Antimicrobial activity, essential oils, UTI, Roasted meatAbstract
In search for alternative ways to control microbial infections, plant essential oils from clove, fenugreek, garlic, neem, eucalyptus and lemongrass were extracted using Soxhlet apparatus and petroleum ether as a solvent. Phytochemical screening of the oils/extracts revealead the presence of alkaloids, flavonoids, saponins, reducing sugars, steroids, tannins, glycosides and triterpenoids. The oils/extracts were then evaluated for their in vitro antimicrobial properties against Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus and Candida albicans of Urinary Tract Infection and meat (suya) origins using disc diffusion and broth dilution techniques. Essential oil from clove was found to have the strongest inhibitory effect being able to inhibit growth of all assayed organisms followed by lemongrass extract, garlic oil, eucalyptus extract and neem oil while essential oil from fenugreek showed no inhibitory effect against all organisms tested. Highest and lowest susceptibility to these oils/extracts was demonstrated by S. aureus (mean zone= 11.7mm) and E. coli (mean zone= 5.8mm) respectively. Organisms isolated from urine showed more resistance than those isolated from meat. The LC50 analysis of the oils/extracts showed relatively high cytotoxic effects against brine shrimps, (<100 ppm). Maximum mortalities took place at a concentration of 1000 ppm whereas least mortalities were at 10 ppm concentration, which means that lethality of the oils/extracts was concentration-dependant.
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Adepoju, T. F., Olawale, O., Okunola, A. A. and Olatunji, E. M. (2014). Solvent Extraction of Oil from Soursop Oil Seeds and its Quality Characterization. International Journal of Sustainable Energy and Environmental Research. 3(2): 82.
Ahmadi, L., Mirza, M. and Shahmir, F. (2002). The Volatile Constituents of Artemisia marschaliana Sprengel and its Secretory Elements. Flavour Fragrance Journal. 17: 141.
https://doi.org/10.1002/ffj.1055
Anjaneyulu, A. S. R., Thomas, R. And Kondaiah, N. (2007). Technologies for Value added Buffalo Meat Products. A Review. American Journal of Food Technology. 2(3): 104-114.
https://doi.org/10.3923/ajft.2007.104.114
Barbosa, L. N., Ral, V. L. M., Fernandes, A. A. H., Ushimaru, P. I., Probst, I. S. and Fernandes, A. J. (2009). Essential Oils Against Foodborne Pathogens and Spoilage Bacteria in Minced Meat. Foodborne Pathogens and Disease. 6(6): 726.
https://doi.org/10.1089/fpd.2009.0282
Brain, K. R. and Turner, T. D. (1975). The Practical Evaluation of Phytopharmaceuticals. Wright Scientenchiea. Bristol. Pp 57-58.
Buchanan, R. E. and Gibbons, N. E. (1974). Bergy's Manual of Determinative Bacteriology. 8th edition. Williams and Wilkins Co., Baltimore.
Cheesbrough, M. (2006). District Laboratory Practice in Tropical Countries Part 2. (2nd edn). Cambridge University Press. Pp 35-244.
https://doi.org/10.1017/CBO9780511543470
Ciulci, I. (1994). Methodology for the Analysis of Vegetable Drugs. Chemical Industries Branch. Division of Industrial Operations, UNIDO. Romania. Pp 82.
Corthout, B., Piefers, L. and Cleays, M. (1992). Antiviral Caffeoylester from Spondias Mombin. Phytochemistry. 31: 1979.
https://doi.org/10.1016/0031-9422(92)80344-E
Fawole, M. O. and Oso, B. A. (2007). Laboratory Manual of Microbiology. 5th edition. Spectrum Books Ltd, Ibadan.
Food Safety Authority of Ireland (2014). Guidelines for the interpretation of results of microbiological testing of ready-to-eat foods placed on the market.
Gafar, M. K., Itodo, A. U., Warra, A. A. and Abdullahi, L. (2012). Extraction and Physicochemical Determination of Garlic (Allium sativum L.) oil. International Journal of Food and Nutrition Science. 1(2): 4-6.
Hablemariam, S., Gray, A. I. and Waterman, P. G. (1993). A New Antibacterial Sesqueterpene from Premma Oligotrichia. Journal of Natural Products. 56: 140-143.
https://doi.org/10.1021/np50091a022
Haman, D. O. (1997). Microbiology of Meat.Food Technology. 23(6): 66.
https://doi.org/10.1046/j.1365-2958.1997.3981777.x
https://doi.org/10.1016/S0167-7012(97)90333-X
Hamza, I. S., Ahmed, S. H. and Aoda, H. (2009). Study of the Antimicrobial Activity of Lemongrass Leaf Extracts. Ministry of Science and Technology. Pp 206.
Harborne, J. B. (1984). Phytochemical Methods: A Guide to Modern Technique of Plant Analysis. 2nd edn. Chapman and Hall, London. Pp 282.
Hassan, I. A., Emun, H. O. And Adekunle, E.O. (2014). Microbiological Quality of Readyto Eat Barbecue Meat (suya) Sold on the Streets of Lagos State. International Journal of Advances in Pharmacy, Biology and Chemistry. 3 (4): 973-5.
Hsueh, P. R., Chen, M. L., Sun, C. C., Chen, W. H., Pan, H. J., Yang, L. S., Chang, S. C., Ho, S. W., Lee, C. Y., Hsueh, W. C. and Luh, K. T. (2002). Antimicrobial Drug Resistance in Pathogens Causing Nosocomial Infections at a University Hospital in Taiwan, 1981- 1999. EmergingInfectious Diseases. 8(1): 63-67.
https://doi.org/10.3201/eid0801.000454
Kang, C. I., Kim, S. H., Bang, J. W., Kim, H. B., Kim, N. J., Kim, E. C., Oh, M. D. and Choe, K. W. (2006). Community Acquired versus Nosocomial K. pneumoniae Bacteremia: Clinical Features, Treatment Outcomes and Clinical Implications of Antimicrobial Resistance. Journal of Korean Medical Science 21: 818-822.
https://doi.org/10.3346/jkms.2006.21.5.816
Khan, R., Islam, B., Akram, M., Shakil, S., Ahmad, A., Ali, S. M., Siddiqui, M. and Khan, A. U. (2009). Antimicrobial Activity of Five Herbal Extracts Against Multi Drug Resistant (MDR) Strains of Bacteria and Fungus of Clinical Origin. Molecules. 14:586-593.
https://doi.org/10.3390/molecules14020586
Krishnaraju, A. V., Rao, T. V. N., Sundararaju, D., Vanisree, M., Tsay, H. and Subbaraju, G. V. (2005).
Assessment of Bioactivity of Indian Medicinal Plants Using Brine Shrimp (Artemia salina) Lethality Assay. International Journal of Applied Science and Engineering. 3(2): 125-134.
Manikandan, C. and Amsath, A. (2016). Prevalence and Distribution of Bacteria and Fungi Isolated from Patients with UTI in Pattukkoccai, Tamilnadu, India. International Journal of Pure and Applied Zoology. 1(3): 213-222.
Meyer, B. N., Ferrigni, N. R., Putnam, J.E., Jacobsen L. B., Nichols D. E. and Mclaughlin, J. L. (1982). Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents. Plant Med. 45: 31-34.
https://doi.org/10.1055/s-2007-971236
Mukhtar, M. D. and Tukur, A. (1999). In vitro Screening for Activity of Pistia stratiotes Extracts. NISEB J. 1(1): 51-60.
National Committee for Clinical Laboratory Standards (NCCLS, 2008). Performance Standards for Antimicrobial Susceptibility Testing: Ninth Informational Supplement. NCCLS document M1090-S9. National Committee for Clinical Laboratory Standards, Wayne, PA.
Olowa, L. F. and Nuneza, O. M. (2013). Brine shrimp Lethality Assay of the Ethanolic Extracts of Three Selected Species of Medicinal Plants from Iligan City, Phillipines. Int. res. J. biological Sci. 2(11): 75.
Owokotomo, I. A., Ekundayo, O. and Dina, O. (2012). Occimum gratissimum: The Brine Shrimps Lethality of a New Chemotype Grown in South Western Nigeria. Global Journal of Science Frontier Research Chemistry. 12(6): 46.
Phan, T. T., Wang, L., See, P., Grayer, R.J., Chan, S. Y. and Lee, S. T. (2001). Phenolic Compounds of Chromolaenaodorata Protect Cultured Skin Cells fom Oxidative Damage: Implication of Cutaneous Wound Healing. Biological and Pharmaceutical Bulletin. 24(12): 1373-1379.
https://doi.org/10.1248/bpb.24.1373
Potdar, K., Gharpure, M. and Wadher, B. (2015). Antimicrobial Activity of E. camaldulensis and C. gigantean Against Various Pathogens. International Journal of Applied Biology and Pharmaceutical Technology. 6(3): 106.39.
Ramachandran, S., Vansikrishna, M., Gowtham, K. V., Heera, B. and Dhanaraju, M. D. (2010).Assessment of Cytotoxic Activity of Agave Cantula using Brine Shrimp Bioassay. Asian Journal of Scientific Research. 4: 90-94.
https://doi.org/10.3923/ajsr.2011.90.94
Richards, M. J., Edwards, J. R., Culver, D. H. and Gaynes, R. P. (1999). Nosocomial Infections in Medical Intensive Care Units in the U.S. National Nosocomial Infections SurvellanceSystem. America.
https://doi.org/10.1542/peds.103.4.e39
Ruden, H., Gastmeier, P. and Daschner, F. D. (1997). Nosocomial and Community Acquired Infections in Germany. Summary of the Results of the First National Prevalence Study (NIDEP). Infection. 25(4): 199.
https://doi.org/10.1007/BF01713142
Sachetti, G., Maletti, S.M., Muzzoli, M., Scaglianti, M., Manfredini, S., Radice,M. and Bruni, R. (2005). Comparative Evaluation of 11 Essential Oils of Different Origin as Functional Antioxidants, Antiradicals and Antimicrobials in Foods. FoodChemistry. 91:621.
https://doi.org/10.1016/j.foodchem.2004.06.031
Scalbert, A. (1999). Antimicrobial Properties of Tannins. Phytochemistry. 30(12): 3875-3883.
https://doi.org/10.1016/0031-9422(91)83426-L
Saide-Alboronoz, J. J., Konipe, C., Maurano, E. A., Beran, W. G. (1995).Contamination of pork carcass during slaughter, fabrication and chilled storage. Journal of Food Protection 58:993-997.
https://doi.org/10.4315/0362-028X-58.9.993
Serban, E. S., Ionescu, M., Matinca, D., Maier, C. S. and Bojita, M. T. (2011). Screening of the antibacterial and antifungal activity of eight volatile essential oils. Farmacia. 59(3): 440-444. Singh, B. R.,
Singh, V., Singh, R. K. and Ebibeni, N. (2011). Antimicrobial Activity of
Lemongrass (C. Citratus) Against Microorganisms of Environmental, Clinical and Food Origin. International Research Jurnal of Pharmacy and Pharmacology. 1 (19):234.
Sofowora, A. (1984). Medicine Plants and Traditional Medicine in Africa. John Wiley and Chichester.
Yusuf, A. M., Abdulhamid, T. H. and Hussain, I. (2012). Isolation and Identification of Bacteria Associated with Balangu (Roasted Meat Product) Sold in Bauchi, Nigeria. IOSR Journal of Pharmacy. 2(6): 38-39.
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