Isolation, Identification and Screening of Humic Acid Producing Fungi from Soil Environment of Oil palm (Elaeisguineensis) Associated with Empty Fruit Bunches

Authors

  • Hassan Musa Kawata Department of Microbiology, Faculty of Pure and Applied Sciences, Kwara State University, Malete, P.M.B 1530, Kwara State, 23431, Nigeria. https://orcid.org/0009-0009-5640-4729
  • P F Omojasola Department of Microbiology, Faculty of Life Sciences, University of Ilorin, Ilorin 1515, Nigeria.
  • A E Ajiboye Department of Microbiology, Faculty of Pure and Applied Sciences, Kwara State University, Malete, P.M.B 1530, Kwara State, 23431, Nigeria.
  • M R Adedayo Department of Microbiology, Faculty of Pure and Applied Sciences, Kwara State University, Malete, P.M.B 1530, Kwara State, 23431, Nigeria.
  • Sheriffdeen Issa Bale Department of Microbiology, Faculty of Sciences, Federal University of Health Sciences, Ila-Orangun, P.M.B 204, Osun Nigeria. https://orcid.org/0000-0002-3876-4634

DOI:

https://doi.org/10.47430/ujmr.2382.019

Keywords:

Humic acid, Fungi, Fermentation, Waste management

Abstract

The demand for sustainable and sustainable biotechnological processes has developed as a result of a growing interest in converting agricultural waste into valuable products. In this study, fungal strainswere isolated from soil environment ofoil palm empty fruit bunchesandscreened for humic acid production. In Osun state, Nigeria, soil sample was taken from a local palm oil mill. Different colonies of fungi were obtained for further research using the serial dilution plating procedure. Plate screening was used to evaluate the fungal biomass as well as colony diameter of the fungal isolates, and a submerged fermentation test was used to measure the amount of humic acid concentration the isolates produced. Fungal isolates were identified by using molecular methods and morphological analysis. The fungi species Aspergillusniger, Rhizopusstolonifer, and Penicilliumchrysogenum were isolated from the soil environment. When compared to Rhizopusstolonifer, Aspergillusniger grew more superiorly in terms of zone diameter (26.5 1.50 mm) and biomass (2.23 0.16 g), while Penicilliumchrysogenum showed no growth. Throughout the fermentation process, Aspergillusnigeralso produced a greater quantity of humic acid, with concentrations varying from 0.70 mg/l to 2.20 mg/l. Aspergillusniger strain HR18's existence was confirmed by molecular analysis. This experiment proved that Aspergillusniger can efficiently produce humic acid from OPEFB, providing a useful method for waste utilization and sustainable practices.

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References

Adewale G. A., Victor T. A., Ebuka C. E. and Kingsley O. I. (2023). Co-carbonization of Waste Biomass with expanded Polystyrene for Enhanced Biochar Production. International Journal of Engineering and Technology, 14 (635-643), https://doi.org/10.1080/17597269.2022.2161133

Al-Jaradi, A., Al-Mahmooli, I., Janke, R., Maharachchikumbura, S., Al-Saady, N., and Al-Sadi, A. M. (2018). Isolation and Identification of Pathogenic Fungi and Oomycetes associated with Beans and Cowpea root Diseases in Oman. Journal of Agricultural Bioscience, 6, e6064. https://doi.org/10.7717/peerj.6064

Ariana, A. and Candra, K. P. (2017). Isolation and Characterization of Lignocellulolytic Microbes from oil palm Empty Fruit Bunches (EFB). Journal of Environment and Sustainability, 1(1), 1–9. https://doi.org/10.22515/sustinere.jes.v10i23.2

Awogbemi, O., Freddie, I., and Idoko, O.E. (2020). Effect of usage on the fatty acid composition and Properties of Neat Palm oil, waste palm oil, and waste palm oil methyl ester. International Journal of Engineering and Technology, 9, 110-. https://doi.org/10.14419/ijet.v9i1.29557

Badis, A., Ferradji, F. Z., Boucherit, A., Fodil, D. and Boutoumi, H. (2019). Characterization and Biodegradation of soil Humic Acids and Preliminary Identification of Decolorizing Actinomycetes at Mitidja Plain Soil (Algeria). African Journal of Microbiology Research, 3(13), 997-1007. https://doi.org/10.5897/AJMR.9000148

Harun, R., Jason, W. S., Cherrington, T., and Danquah, M. (2016). Microalgal Biomass as Cellulosic Fermentation Feedstock for Bioethanol Production. Renewable and Sustainable Energy Reviews. https://doi.org/10.1016/j.rser.2010.07.071

Kluczek-Turpeinen, B., Steffen, K., Tuomela, M., Hatakka, A., and Hofrichter, M. (2005). Modification of Humic acids by the Compost-dwelling Deutermycete paecilomyces Inflatus. Applied Microbiology and Biotechnology, 66(4), 443-449. https://doi.org/10.1007/s00253-004-1677-4

Motta F. L. and Santana M.H.A (2023). Solid-state Fermentation for Humic Acids Production by a Trichoderma reesei Strain using an Oil Palm Empty Fruit Bunch as the Substrate. Journal of Applied Biochemical Biotechnology.172:2205–2217. https://doi.org/10.1007/s12010-013-0668-2.

Motta, F. L., and Santana, M. H. A. (2012). Biomass Production from Trichoderma viride in Nonconventional Oat Medium. 28, 1245–1250. https://doi.org/10.1002/btpr.1578.

Motta, F. L., and Santana, M. H. A. (2013). Production of Humic Acids from oil palm Empty Fruit Bunch by Submerged Fermentation with Trichoderma viride: Cellulosic Substrates and Nitrogen Sources. 29, 631–637. https://doi.org/10.1002/btpr.1715.

Naranjo, L., Urbina, H., De Sisto, A. and Leon, V. (2007). Isolation of autochthonous non-white rot Fungi with Potential for Enzymatic Upgrading of Venezuelan Extra-heavy crude oil. Journal of Biocatalysis and Biotransformation, 25(2-4), 341–349. https://doi.org/10.1080/10242420701379908

Rahim, I., Suherman, S., and Nasaruddin, A. (2019). The Diversity of Rot Fungi from Cocoa Plantation and Its Ability to Grow on Carbon Source Media. Planta Tropika: Journal of Agriculture, 7, 125–129. https://doi.org/10.18196/pt.2019.102.125-129

Saeed, Q., Xiukang, W., Haider, F. U., Kučerik, J., Mumtaz, M. Z., Holatko, J., Naseem, M., Kintl, A., Ejaz, M., Naveed, M., Brtnicky, M., and Mustafa, A. (2021). Rhizosphere Bacteria in Plant Growth Promotion, Biocontrol, and Bioremediation of Contaminated Sites: A Comprehensive Review of Effects and Mechanisms. International Journal of Molecular Sciences, 22(19), 10529. https://doi.org/10.3390/ijms221910529

Sulaiman, F., Abdullah, N., Gerhauser, H., and Shariff, A. (2011). An Outlook of Malaysian Energy, Oil Palm Industry and Its Utilization of Wastes as Useful Resources. Biomass Bioenergy, 35, 3775–3786. https://doi.org/10.1016/j.biombioe.2011.06.018

Tamura K., Stecher G., and Kumar S. (2021). MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution https://doi.org/10.1093/molbev/msab120.

Tao, H., Sheddon, J.L., Slade, E.M., Ludovic, H., Cul, J.R. and Willis, K.J. (2018). Application of Oil Palm Empty Fruit Bunch effects on Soil Biota and Functions: A case study in Sumatra, Indonesia. Agriculture, Ecosystem and Environment; Vol. 56 p105-113. https://doi.org/10.1016/j.agee.2017.12.012

Victor, O. D., Adegboyega, O.F. and Victor, O.A.(2021). Morphological and Molecular Characterization of Aspergillus niger Causing Postharvest rot of White Yam (Dioscorea rotundataPoir), Archives of Phytopathology and Plant Protection. 54:19-20, 2356-2374, https://doi.org/10.1080/03235408.2021.1983365

Volpi, M. P. C., Corzo, I. J. M., Bastos, R. G., & Santana, M. H. A. (2019). Production of Humic Acids by Solid-state Fermentation of Trichoderma reesei in Raw Oil Palm Empty Fruit Bunch Fibers. Journal of Biotechnology. 9(11), 393. https://doi.org/10.1007/s13205-019-1925-z

Volpi, M. P. C., Santos, V. S., Badan, A. P. R., Santana, M. H., and Bastos, R. G. (2018). The Role of Lignocellulosic Composition and Residual Lipids in Empty Fruit Bunches on the Production of Humic Acids in Submerged Fermentations. Journal of Applied Biochemical Biotechnology, 3, 957–964. https://doi.org/10.1007/s12010-018-2850-z.

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Published

30-12-2023

How to Cite

Kawata, H. M., Omojasola, P. F., Ajiboye, A. E., Adedayo, M. R., & Bale, S. I. (2023). Isolation, Identification and Screening of Humic Acid Producing Fungi from Soil Environment of Oil palm (Elaeisguineensis) Associated with Empty Fruit Bunches. UMYU Journal of Microbiology Research (UJMR), 8(2), 165–173. https://doi.org/10.47430/ujmr.2382.019

Issue

Vol. 8 No. 2 (2023): UMYU Journal of Microbiology Research (UJMR), Volume 8, Number 2, December 2023

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