Diagnosis of Malaria among Children in Sokoto: A Comparison of Microscopy and Rapid Diagnostic Tests
DOI:
https://doi.org/10.47430/ujmr.2491.029Keywords:
Children, Malaria, Microscopy, Rapid Diagnostic TestsAbstract
Study’s Novelty/Excerpt
- This study compares the utility, sensitivity, specificity, and predictive values of microscopy with that of Rapid Diagnostic Tests (RDTs) in diagnosing malaria among children in Specialist Hospital Sokoto.
- The novelty lies in its focus on a specific pediatric population in a resource-limited setting, providing valuable data on the comparative effectiveness of these diagnostic methods in real-world clinical practice.
- The findings highlight the higher positivity rate of microscopy, advocating for its continued use alongside RDTs to ensure accurate malaria diagnosis and optimal patient care in similar environments.
Full Abstract
Malaria is a life-threatening disease primarily found in tropical countries, and it is the leading cause of morbidity and mortality among children. Diagnosis of malaria depends largely on clinical presentations and laboratory diagnosis. Microscopy is the gold standard for laboratory malaria diagnosis but requires adequate training and time compared to Rapid Diagnostic Tests (RDTs). The study compared the utility, sensitivity, specificity, and predictive values between microscopy and RDTs in diagnosing malaria among children accessing care in Specialist Hospital Sokoto. A total of 367 blood samples of consented children who met the study inclusion criteria were examined. All samples were screened for malaria using RDT thin and thick blood films. Of the 367 samples assessed, RDT was positive for 202 (55.0%) and negative for 165 (45.0%), while microscopy was positive for 235 (64.1%) and negative for 132 (35.9%), a non-statistically significant (χ2 = 0.090, P = 0.922) difference was observed when both positive tests were compared. The Rapid diagnostic tests (RDTs) showed a sensitivity of 85.95% and a specificity of 83.33%. This study confirms the higher positivity rate of microscopy to RDTs in diagnosing malaria. As such, RDTs are useful for rapid malaria diagnosis, especially in resource-limited settings; microscopy should be encouraged as much as possible for children to avoid missing any positive cases.
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References
Abanyie, F. A., Arguin, P. M., and Gutman, J. (2011). State of malaria diagnostic testing at clinical laboratories in the United States: A nationwide survey. Malaria Journal; 10(340): 1-10. https://doi.org/10.1186/1475-2875-10-340
Abeku, T. A., Kristan, M., Jones, C., Beard, J., Mueller, D. H., and Okia, M. (2008). Determinants of the accuracy of rapid diagnostic tests in malaria case management: Evidence from low and moderate transmission settings in the East African highlands. Malaria Journal: 7(202); 1-10. https://doi.org/10.1186/1475-2875-7-202
Acheampong, D. O., Appiah, M. G., Boamponsem, L. K., ABoampong, J. N. and Afoakwah, R. (2011). The efficacy of Rapid Diagnostic Test (RDT) in diagnosing Plasmodium falciparum malaria in some selected Health facilities in the cape coast metropolis of Ghana. Advanced Applied Science and Research; 2(1): 348-356
Amexo, M., Tolhurst, R., Barnish, G. and Bates, I. (2004). Malaria misdiagnosis: Effects on the poor and vulnerable. Lancet: 364(9448); 1896-1898. https://doi.org/10.1016/S0140-6736(04)17446-1
Azikiwe, C. C., Ifezulike, C. C., Siminialayi, I. M., Amazu, L. U., Enye, J. C. and Nwakwunite, O. E. (2012). A comparative laboratory diagnosis of malaria: Microscopy versus Rapid Diagnostic Test kits. Asian Pacific Journal of Tropical Medicine; 2(4): 307-310. https://doi.org/10.1016/S2221-1691(12)60029-X
Batwala, V., Magnussen, P. and Nuwaha, F. (2011). Comparative feasibility of implementing Rapid Diagnostic Test and Microscopy for parasitological Diagnosis of malaria in Uganda. Malaria Journal; 19(10): 373. https://doi.org/10.1186/1475-2875-10-373
Cheesbrough, M. (2006). District Laboratory Practice in Tropical Countries Part 1 Cambridge, 2nd Edition. Published by Press Syndicate of the University of Cambridge: 247-258. https://doi.org/10.1017/CBO9780511543470
Elechi, H. A., Rabasa, A. I., Bashir, M. F., Gofama, M. M., Ibrahim, H. A. and Askira, U. M. (2015). Uncomplicated malaria in children: The place of Rapid Diagnostic Test. Niger Medical Journal; 56(2): 85-90. https://doi.org/10.4103/0300-1652.150686
Faucher, J. F., Aubouy, A., Beheton, T., Makoutode, P., Abiou, G. and Doritchamou, J. (2010). What would PCR assessment change in the management of fevers in a malaria endemic area? A school based study in Benin in children with and without fever. Malaria journal 9: 224. https://doi.org/10.1186/1475-2875-9-224
Garba, I. B., Aminu, S. M., Abdullahi, M., Bassey, E., Ibrahim, Y., Nura, K. B., Akeem, O. A. and Taofik, K. (2016). Diagnosis of malaria: A comparison between microscopy and Rapid Diagnostic Test (RDT) among under-five children at Gusau Nigeria. Sub-Saharan Africa Journal of Medicine: 3(2); 96-101. https://doi.org/10.4103/2384-5147.184371
Gilles, H. M. and Warrell, D. A. (1993). In: Bruce-Chwatt’s Essential Malariology, 3rd Edition Edward Arnold, 19-124
Harchut, K., Standley, C., Dobson, A., Klaassen, B., Rambaud-Althaus, C. and Althaus, F. (2013). Over-diagnosis of malaria by microscopy in the Kilombero Valley, Southern Tanzania: An evaluation of the utility and cost-effectiveness of Rapid Diagnostic Tests. Malaria Journal: 12(159); 1-9. https://doi.org/10.1186/1475-2875-12-159
Kurup, R. and Marks, R. (2012). A comparison of microscopic examination and Rapid Diagnostic Tests (RDT) used in Guyana to diagnose malaria. Malaria Reports: 2(2); 1-5. https://doi.org/10.4081/malaria.2012.e2
Mohammed, K., Aminu, U., Spencer, T. H. I., Ibrahim, K. K., Ikeh, E. I., Nataala, S. U. and Garba M. K. (2017). Comparison and Evaluation of Asymptomatic Malaria Parasitaemia among Pregnant Women Attending Specialists Hospital, Sokoto, North-Western, Nigeria. International Journal of tropical disease and Health 23(4): 1-9. https://doi.org/10.9734/IJTDH/2017/32599
National Population Commission (2012), National Malaria Control Programme, and ICF International. Nigeria Malaria Indicator Survey, Abuja; 2012.
Ocheje, A. J. and Dogara, M. M. (2016). Prevalence of malaria and risk factors among patients attending Dutse General Hospital, Jigawa State, Nigeria. International Research Journal of Public and Environmental Health: 3(11); 270-277
Ojurongbe, O., Adegbosin, O. O., Taiwo, S. S., Alli, O. A., Olowe, O. A., Ojurongbe, T. A. Bolaji O. S., Adeyeba, O. A. (2013). Assessment of clinical diagnosis, microscopy, Rapid Diagnostic Tests, and Polymerase chain reaction in the diagnosis of Plasmodium falciparum in Nigeria. Malaria Research and Treatment :308-369. https://doi.org/10.1155/2013/308069
Reyburn, H., Mbakilwa, H., Mwangi, R., Mwerinde, O., and Olomi, R. (2007) Rapid Diagnostic tests compared with malaria microscopy for guiding outpatient treatment of febrile illness in Tanzania: Randomised trial. Biomedical Journal: 334; 403. https://doi.org/10.1136/bmj.39073.496829.AE
Sani, U. M., Jiya, N. M. and Ahmed, H. (2013). Evaluation of a malaria Rapid Diagnostic Test among febrile children in Sokoto, Nigeria. International Journal of Medical lab Science 3:434-440
Taofiq, S., Bunza, M. D., Majeed, Q., Abubakar, M. B. and Ladan, M. U. (2017). Study on Snail Vectors of Helminth Disease Agents along Rima River Valley at Kwalkwalawa Village, Wamakko Local Government Area, Sokoto State, Nigeria. Tropical Medicine journal 2(1): 1-5. https://doi.org/10.36876/smjhrt.1011
Uzochukwu, B. S., Obikeze, E. N., Onwujekwe, O. E., Onoka, C. A. and Griffiths, U. K. (2009). Cost-effectiveness analysis of Rapid Diagnostic Test, microscopy and syndromic approach in the diagnosis of malaria in Nigeria: Implications for scaling-up deployment of ACT. Malaria Journal; 8: 265. https://doi.org/10.1186/1475-2875-8-265
Weber, M. W., Zimmermann, U., Van Hensbroek, M. B., Frenkel, J. and Palmer, A. (1999) Renal involvement in Gambian children with cerebral or mild malaria. Tropical Medicine and International Health:4; 390-394. https://doi.org/10.1046/j.1365-3156.1999.00409.x
WHO (2010). Guidelines for the Treatment of Malaria. 2nd edition Geneva, Switzerland.
WHO (2014) World Malaria Report 2013. World Health Organization, Geneva, Switzerland
Wongsrichanalai, C., Barcus, M. J., Muth, S., Sutamihardja, A. and Wernsdorfer, W. H. (2007). A review of malaria diagnostic tools: Microscopy and Rapid Diagnostic Test (RDT). American Journal of Tropical Medicine and Hygiene; 77 (6):119 127. https://doi.org/10.4269/ajtmh.2007.77.119
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