Bio-Prospecting Xylose-Utilizing, Exopolysaccharide (EPS)-Producing Bacteria and EPS Quantification through Submerged Fermentation using Xylose as the Major Carbon Source


  • Antia, U. Department of Microbiology, Akwa Ibom State University, Mkpat Enin, 524106, Nigeria
  • Stephen, N. Department of Microbiology, Akwa Ibom State University, Mkpat Enin, 524106, Nigeria
  • Umoh, V. Department of Microbiology, Akwa Ibom State University, Mkpat Enin, 524106, Nigeria
  • Bassey, M. Department of Microbiology, Akwa Ibom State University, Mkpat Enin, 524106, Nigeria
  • Udo, I. Department of Microbiology, University of Uyo, Uyo, 520101, Nigeria
  • Adeleke, A. J. Department of Microbiology, Modibbo Adama University, Yola, 640230, Nigeria



Bioprospecting, exopolysaccharides, lignocellulosic biomass, submerged fermentation, xylose


Study’s Novelty Excerpt

  • This study presents a novel investigation into the ability of bacteria to utilize xylose, a pentose sugar, for exo-polysaccharide (EPS) production, addressing a significant gap in current research which predominantly focuses on hexose-utilizing bacteria.
  • By isolating and identifying EPS-producing bacteria from diverse environmental sources, the research highlights the potential of species such as Enterobacter cloacae and Klebsiella oxytoca to produce significant quantities of EPS using xylose as the sole carbon source.
  • The findings demonstrate the feasibility of employing alternative carbon sources for EPS production, with implications for enhanced biotechnological applications across multiple industries.

Full Abstract

Many microorganisms are capable of producing Exo-polysaccharides (EPS) while utilizing simple sugars and hexoses. These EPS found applications in various fields, such as agricultural biotechnology, pharmaceuticals, textiles, and food industries. However, there is a lack of studies on EPS-elaborating bacteria that can utilize pentoses like xylose. Therefore, the utilization of alternative carbon sources for EPS production has become a focus of recent research. This study aimed to prospect bacteria that can utilize xylose for EPS production. Samples from agricultural soil, dump sites, saline soil, cement-contaminated soil, fresh cow milk, cow dung, and yogurt were serially diluted and cultured in a salt-based medium with xylose as the primary carbon source. Slimy and mucoid colonies were selected as potential EPS-producing isolates and identified morphologically and biochemically using the VITEK 2 Automated identification system. The quantification of EPS production by these isolates was conducted through submerged fermentation with xylose as the sole carbon source. The mean heterotrophic bacterial count of xylose-utilizing bacteria ranged from 2.1x106 CFU to 3.5x108 CFU per gram of the samples analyzed. The slimy and mucoid colonies were identified as members of the genera Staphylococcus, Enterobacter, Kocuria, Klebsiella, Enterococcus, Serratia, and Burkholderia. The quantities of EPS produced by the isolates ranged from 0.04 g/L to 2.0 g/L, with E. cloacae D1, E. cloacae D2, K. oxytoca D2, and K. oxytoca G1 elaborating the highest amount of EPS. Bacterial isolates capable of utilizing xylose for EPS production were obtained from various sources, showing potential for further optimization


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Abdeshahian, P., Ascencio, J. J., Philippini, R. R., Antunes, F. A. F., Dos Santos, J. C., and da Silva, S. S. (2020). Utilization of sugarcane straw for production of β-glucan biopolymer by Lasiodiplodia theobromae CCT 3966 in batch fermentation process. Bioresource Technology, 314: 123716.

AMAO, J. A., Omojasola, P. F. and Barooah, M. (2019). Isolation and characterization of some exopolysaccharide producing bacteria from cassava peel heaps. Scientific African, 4: e00093.

Antia, U. E., Akan, O. D., Stephen, N. U., Eno-Ibanga, C. K., and Akpan, N. G. (2018). Isolation and Screening of Yeast Isolates Indigenous Palm Wine for Ethanol Production. Philippine Journal of Science, 147(3).

Anita, U. E., Stephen, N. U., Onilude, A. A., and Ibanga, I. A. (2019). Studies of the Nutritional, Environmental Effects and Repressive Nature of Simple Sugars on the Production of endo-β-mannanase by Aspergillus flavus PT7 on Solid State Fermentation. Journal of Advanced Biology, 21(4): 1-12.

Antia, U. E., Stephen, N. U., Onilude, A. A., Udo, I. O. M., & Amande, T. J. (2023).Bioconvertibility of mannan-containing polysaccharides to bioethanol: A comparative study of palm kernel cake and copra meal feedstocks. Biomass Conversion and Biorefinery, 13(6): 5175-5186.

Chowdhury, S. (2012). Heterotrophic bacteria in drinking water distribution system: are view. Environmental monitoring and assessment, 184: 6087-6137.

Donot, F., Fontana, A., Baccou, J. C., and Schorr-Galindo, S. (2012). Microbial exopolysaccharides: main examples of synthesis, excretion, genetics and extraction. Carbohydrate Polymers, 87(2): 951-962.

Fretias, F., Alves, V. D., and Reis, M. A. (2011). Advances in bacterial exopolysaccharides:from production to biotechnological applications. Trends in biotechnology, 29(8): 388-398.

George, A. E., Antia, U. E., Adeleke, A. J., and Fatunla, O. K. (2023). Optimisation of Polyhydroxy Butyrate Production by Lysinibacillus fusiformis and Metabacillus indicus isolated from Spent Engine-oil Contaminated Soil. UMYU Journal of Microbiology Research (UJMR), 8(2): 30-39.

Jazini, M. H., Fereydouni, E., and Karimi, K. (2017). Microbial xanthan gum production from alkali pretreated rice straw. RSC advances, 7(6): 3507-3514.

Jesus, M., Araújo, P., Silva, T., Reis, E., Ruzene, D., Silva, D., and Padilha, F. (2014). Evaluation of production of xanthan gum utilizing the corn cob liquor as a carbon source in different strains of Xanthomonas campestres. In BMC Proceedings, 8(4): 1-2.

Kumar, A. S., Mody, K., and Jha, B. (2007). Bacterial exopolysaccharidesa perception. Journal of Basic Microbiology, 47: 103.

Ling, T.K, Liu, Z. K. and Cheng, A.F. (2003). Evaluation of the VITEK 2 system for rapid direct identification and susceptibility testing of gram negative bacilli from positive blood cultures. Journal of Clinical Microbiology, 41: 4705-4707.

Mende, S., Rohm, H., and Jaros, D. (2016). Influence of exopolysaccharides on the structure, texture, stability and sensory properties of yoghurt and related products. International Dairy Journal, 52: 57-71.

Mishra, A. and Jha, B. (2013). Microbial Exopolysacchrides. In: Rosenberg, E, DeLong, E. F., Thompson, F., Lory, S., Stackebrandt, E. (Eds.), The Prokaryotes: Applied Bacteriology and Biotechnology, 4th ed. Springer Berlin Heidelberg, pp. 179-192

Moscovici, M. (2015). Present and future medical applications of microbial exopolysaccharides. Frontiers in Microbiology, 6, 137616.

Mu'Minah, Baharuddin, Subair, H., and Fahruddin. (2015). Isolation and Screening Bacterial Exopolysaccharide (EPS) from Potato Rhizosphere in Highland and the Potential as a Producer Indole Acetic Acid (IAA). Procedia Food Science, 3: 74-81.

Nadzir, M. M., Nurhayati, R. W., Idris, F. N., and Nguyen, M. H. (2021). Biomedical Applications of Bacterial Exopolysaccharides: A Review. Polymers, 13(4).

Nguyen, T., Nguyen, T., Bui, C., Hong, T., Hoang, K., and Nguyen, T. (2020). Exopolysaccharide production by lactic acid bacteria: The manipulation of environmental stresses for industrial applications. AIMS Microbiology, 6(4): 451-469.

Netrusov, A.I., Liyaskina, E. V., Kurgaeva, I. V., Liyaskina, A. U., Yang, G., Revin, V. V. (2023) Exopolysaccharides Producing Bacteria: A Review. Microorganisms, 11(6): 1541. PMID: 37375041; PMCID: PMC10304161.

Oner, E. T. (2013). Microbial production of extracellular polysaccharides from biomass. In: Z. Fang, (Ed) Pretreatment techniques for biofuels and biorefineries. Springer, Berlin, pp 35-56.

Onilude, A. A., Fadahunsi, I. F., Antia, U. E., Garuba, E. O., & Ja'afaru, M. I. (2012). Characterization of crude alkaline β-mannosidase produced by Bacillus sp. 3A isolated from degraded palm kernel cake. AU Journal of Technology, 15(3).

Palomba, S., Cavella, S., Torrieri, E., Piccolo, A., Mazzei, P., Blaiotta, G., Ventorino, V., Pepe, O. (2012). Polyphasic screening, homopolysaccharide composition, and viscoelastic behavior of wheat sourdough from a Leuconostoc lactis and Lactobacillus curvatus exopolysaccharide-producing starter culture. Applied Environmental Microbiology, 78: 2737-47.

Ramírez-Castillo, M.L. and Uribelarrea, Jean-Louis. (2004). Improved process for exopolysaccharide production by Klebsiella pneumoniae sp. pneumoniae by a fed-batch strategy. Biotechnology letters, 26: 1301-6.

Suryawanshi, N., Naik, S. and Jujjawarapu, S. (2022). Exopolysaccharides and their Applications in Food Processing Industries. Food Science and Applied Biotechnology, 5: 22. 10.30721/fsab2022.v5.i1.165.

Torres, C. A., Antunes, S., Ricardo, A. R., Grandfils, C., Alves, V. D., Freitas, F., and Reis, M. A. (2012). Study of the interactive effect of temperature and pH on exopolysaccharide production by Enterobacter

A47 using multivariate statistical analysis. Bioresource Technology, 119: 148-156.

Ventorino, V., Nicolaus, B., Di Donato P., Pagliano, G., Poli, A., Robertiello, A., Iavarone V. and Pepe. O. (2019). Bioprospecting of exopolysaccharide-producing bacteria from different natural ecosystems for biopolymer synthesis from vinasse. Chemical and Biological Technologies in Agriculture, 6: 1-9.

Vijayabaskar, P., Babinastarlin, S., Shankar, T., Sivakumar, T., and Anandapandian, K. T. K. (2011). Quantification and characterization of exopolysaccharides from Bacillus subtilis (MTCC 121). Advance Biological Resource, 5(2): 71-76.

Wang, D., Ju, X., Zhou, D., and Wei, G. (2014). Efficient production of pullulan using rice hull hydrolysate by adaptive laboratory evolution of Aureobasidium pullulans. Bioresource Technology, 164: 12-19.

Wu, J., Zhang, Y., Ye, L., and Wang, C. (2021). The anti-cancer effects and mechanisms of lactic acid bacteria exopolysaccharides in vitro: A review. Carbohydrate polymers, 253: 117308.

Zhao, Z., Xian, M., Liu, M., and Zhao, G. (2020). Biochemical routes for uptake and Conversion of xylose by microorganisms. Biotechnology for biofuels, 13: 1-12.




How to Cite

Antia, U., Stephen, N., Umoh, V., Bassey, M., Udo, I., & Adeleke, A. J. (2024). Bio-Prospecting Xylose-Utilizing, Exopolysaccharide (EPS)-Producing Bacteria and EPS Quantification through Submerged Fermentation using Xylose as the Major Carbon Source. UMYU Journal of Microbiology Research (UJMR), 13–21.