Evaluation of Stool Concentration Techniques in the Detection of some Parasites among Almajiri School Children in Sokoto Metropolis

Authors

  • Garba, I. Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto. https://orcid.org/0000-0001-6232-5044
  • Umar, A.I. Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto.
  • Tijjani, M.B. Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria.
  • Aliyu, M.S. Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria.
  • Doko, M.H.I. Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria.
  • Raji, M.I.O. Department of Pharmaceutics and Pharmaceutical Microbiology, Usmanu Danfodiyo University, Sokoto.
  • Udefi, A.C. Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto.

DOI:

https://doi.org/10.47430/ujmr.2381.016

Keywords:

Almajiri school, Concentration, Techniques, Sokoto, Stool

Abstract

Infection with intestinal parasites is one of the main health issues in poor nations. It is estimated to affect 3.5 billion people worldwide, and 450 million people are thought to be ill as a result of such illnesses, with children making up the majority of those who are ill. By using several stool concentration techniques, this study was aimed at identifying parasite ova in the stool of Almajiri school children in Sokoto.  The Formol-ether concentration technique, which is regarded as the gold-standard, Sheather's sugar floating technique, and saline floatation technique were employed to detect parasite ova in stool samples taken from Almajiri school children. A total of 137 stool samples were collected from Almajiri school children and each sample was examined by these concentration techniques. A prevalence of 37.9% of intestinal helminthiasis was observed by Formol-ether concentration technique. The sensitivity and specificity of Formol-Ether concentration technique were 96.2% and 97.8% respectively, followed by Sheather’s sugar floating technique 88.5% and 93.8% and the lowest sensitivity and specificity of 76.9% and 90% respectively were obtained in the saline floatation technique. There was a high prevalence of intestinal helminthiasis among Al-majiri school children, sheather’s sugar floatation technique was as sensitive as the Formol-ether concentration technique and more sensitive than the Saline floatation technique. Therefore, Sheather’s sugar floatation technique is recommended as a better alternative in the diagnosis of intestinal helminthiasis.

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References

Acharya, T. (2012). Formal-ether sedimentation technique for the concentration of stool parasites and Laboratory diagnosis of parasitic disease. Retrieved on 2nd March,2018, From http://.microbeonline.com/formol-ether-sedimentation-techniques/.

Baratloo, A., Hosseini, M., Ahmed Negida, A. and El Ashal, G. (2015). Simple Definition and Calculation of Accuracy, Sensitivity and Specificity, Emergency 3(2): 48-49.

Centers for Disease Control and Prevention (CDC). (2013). Soil-transmitted helminths: Global health division of parasitic diseases. Available at www.cdc.gov/parasites/sth/.Retrieved on 3rd March, 2018.

Centers for Disease Control and Prevention (CDC). (2017). Helminths, Soil-Transmitted: Infectious diseases related to travel. Available at www.cdc.gov/parasites/sth/.Retrieved on 3rd March, 2018.

Cheesbrough, M. (2009). District laboratory practice in tropical countries(part-1) (2ndedn). England: Cambridge University Press, pp. 209-235

Chigozie, J., Kelvin, O., E., Patrick, G., O., Nelsonm, C., A. and Emmanuel, A., (2007). Soil transmitted Helminth infection in school children in Eastern Nigeria; the public health implication. International Journal of third world medicine. (4)1.https://doi.org/10.5580/3f7

Enimien O. J., Fana S. A. and Emmanuel W. B., (2014). Intestinal helminthic infection in Numan (Northeast Nigeria). International Journal of Innovation and Applied Studies5(2): 102-105.

Gashaw F., Aemero M., Legesse M., Petros B., Teklehaimanot T., Medhin G., Berhe N., Mekonnen Y. and Erko B. (2015). Prevalence of Intestinal Helminth infection among school children In Maksegnit and Enfranz Towns, Northwestern Ethiopia, with Emphasis on Schistosoma mansoni Infection. Parasites and Vectors.8(567): 1-8.https://doi.org/10.1186/s13071-015-1178-6

Hedley, L., Robert, L. and Wani, S. (2015). Helminth infections: diagnosis and treatment. The pharmaceutical journal.295(962): 1-3.

Ibrahim, T. (1997) Sample size determination In Research Methodology and Dissertation writing for health and allied health professionals (1stEdn). Nigeria: Lucas, A.O, pp. 74-75.

Iduh, M. U Mohammed K., Isah I., Ashcroft O. F., Garba M. K and Nataala, S. U (2021). Gastro Intestinal Helminths among Hausa-Fulani inWamakko and Tambuwal Local Government Area of Sokoto, Nigeria. International Journal of Pathogen Research, 7(4): 22-37;https://doi.org/10.9734/ijpr/2021/v7i430189

Jamala U.L., Eze N.C. and Nduka F.O. (2018). Prevalence and intensity of intestinal helminth infections and associated risk factors among school-aged children in Abua/Odual Local Government Area, River state. Journal of Applied Life Sciences International.16(2): 1-7.https://doi.org/10.9734/JALSI/2018/39106

Manser, M.M., Saez, A.C.S. and Chiodini, P.L. (2016). Faecal parasitology: concentration methodology needs to be better standardized. Neglected Tropical Diseases10(4): 1-16.https://doi.org/10.1371/journal.pntd.0004579

Mergani H.M., Mohammed A.S., Nawed K., Meraj B. and Khan A. (2014). Detection of Intestinal Protozoa by using Different methods.Journal of dentistry and medical research; 2(2): 28-32.https://doi.org/10.4103/2348-1471.143326

Mohammed, M., Vantsawa P.A., Abdullahi, U.Y. and Muktar, M.D. (2015). Nutritional Status and Prevalence of Intestinal Schistosomiasis among Al-majiriPopulation in Kawo District of Kaduna Metropolis, Kaduna State-Nigeria.Journal of Bacteriology and Parasitology, 6(4): DOI: 10.4172/2155-9597.1000237.https://doi.org/10.4172/2155-9597.1000237

Obiukwu, M., O.,Umeanaeto, P., U.,Eneanya, C., I. and Nwaorgu, G., O. (2008), Prevalence of gastrointestinal helminths in school children in Mbaukwu, Anambra State, Nigeria.Nigeria journal of parasitology, 29(1), pp 15-19.https://doi.org/10.4314/njpar.v29i1.37897

Odinaka, K.K., Nwolisa, E.C., Mbanefo, F., Iheakaram,A.C. and Okolo, S. (2015). "Prevalence and Pattern of Soil-Transmitted Helminthic Infection among Primary School Children in a Rural Community in Imo State, Nigeria", Journal of Tropical Medicine, vol. 4https://doi.org/10.1155/2015/349439

Parameshwarappa, K.D., Chandrakanth C. and Sunil, B. (2012). The prevalence of intestinal parasitic infestations and the evaluation of different concentration techniques of stool examination.Journal of clinical and diagnostic research.6(7): 1188-1191.

Rojekittikhun, W., Mahittikorn, A., Prummongkol, S., Puangsa-art S., Chaisiri, K. and Kusolsuk T. (2015). Evaluation of Sugar Flotation and Formalin-Ether Concentration Techniques in the Examination of GI Parasites of Refuge Dogs and Cats in Kanchanaburi Province, Thailand. Journal Of Tropical Medicine and Parasitology, 38(1): 17-24.

Salam N. and Azam S. (2017). Prevalence and distribution of soil-transmitted helminth infections in India: Journal of Public Health. 17(201): 1-12.https://doi.org/10.1186/s12889-017-4113-2

Tegegne Y., Wondmagegn T., Worku L. and Zeleke A.J. (2018). Prevalence of Intestinal Parasites and Associated Factors among Pulmonary Tuberculosis Suspected Patients Attending University of Gondar, Northwest Ethiopia: Journal of Parasitology Research 2018(9372145): 1-6.https://doi.org/10.1155/2018/9372145

Tille, P.M. and Forbes, B.A. (2014). Bailey and Scott's Diagnostic Microbiology(13thedn) St. Louis, Missouri: Elsevier, pp. 656-663.

Wichit, R., Aongart, M., Samrerng, P., Supalarp, P., Kittipong, C. and Teera, K. (2015). Evaluation of Sugar Flotation and Formalin-Ether Concentration Techniques in the Examination of GI Parasites of Refuge Dogs and Cats in Kanchanaburi Province, Thailand.Journal of Tropical Medical Parasitology, 38: 17-24.

Yahaya, A., Yakubu, B., T. and Aisha, I. (2015). Prevalence of intestinal Parasitic Helminths from Finger nails of "Almajiris" in Birnin Kudu local Government Area, Jigawa state, Nigeria. International Journal of Tropical Disease & Health, 8(2): 66-74https://doi.org/10.9734/IJTDH/2015/14186

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Published

30-06-2023

How to Cite

Garba, I., Umar, A.I., Tijjani, M.B., Aliyu, M.S., Doko, M.H.I., Raji, M.I.O., & Udefi, A.C. (2023). Evaluation of Stool Concentration Techniques in the Detection of some Parasites among Almajiri School Children in Sokoto Metropolis. UMYU Journal of Microbiology Research (UJMR), 8(1), 127–133. https://doi.org/10.47430/ujmr.2381.016

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