Use of Tomato Juice Supplemented With Glucose as a Medium for Growing Fungi
DOI:
https://doi.org/10.47430/ujmr.1721.030Keywords:
tomato juice, fungi, PDA, glucoseAbstract
In this study, the possibility of using tomato juice as an alternative medium for growing fungi was tested. Four isolates; Penicillium sp., Aspergillus sp., Fusarium sp. and Candida sp. were inoculated on Tomato Juice Agar (TJA), Tomato Juice Agar supplemented with 1g of glucose (TJA+1g Glc), Tomato Juice Agar supplemented with 2g of glucose (TJA + 2g Glc) and Potato Dextrose Agar (PDA) as control medium. The diameters of the fungal colonies were measured at 24 hours intervals for four days. The fungal growths on the test media were compared with those on PDA. The results for Fusarium sp., Penicillium sp. and Asprgillus sp. were significantly higher (P < 0.05) on TJA than PDA. This study shows that tomato juice agar (TJA) is suitable for the growth of all the tested fungi and can be used as an alternative medium for growing fungi.
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References
Adesemoye, A.O.and Adedire C.O. (2005).Use of cereals as basal medium for the formulation of alternative culture media for media Word Journal of Microbiology and Biotechnology, 21: 329-336.
https://doi.org/10.1007/s11274-004-3907-4
Adesina, G.O. and Akinyosoye, F.A. (2006). Effect of fresh and fermenting cassava on the Growth of microbial isolates from cassava. Nigerian Journal of Microbiology, 20:971-979.
Alves, S. H., Loreto, E.S., Linares, C.E., Silvera, L.A., Pereira, D.I.B. and Santurio, J.M. (2006). Comparison among tomato juice agar with other three media for differentiation of Candida dubliniensis from Candida albicans. Reversed institute of medical tropical San panlo, 48(3): 119-121.
https://doi.org/10.1590/S0036-46652006000300001
Black,J.G. (2004). Microbiology, Principle and Explorations. 6th ed. John Wiley and Sons, inc. Marymount University, Arlingtons, Virginia. Pp. 308-313.
Famurewa, O and David, O.M. (2008). Formulation and evaluation of dehydrated microbiological media from Avocado pear (Peasea americana). Research Journal of Microbiology, 3:326-330.
https://doi.org/10.3923/jm.2008.326.330
Ikenebomen, M.J. and Chikwendu, A.E. (1997).Aspergillus niger biomass production in Cassava whey medium.Nigerian Journal of Microbiology, 2:51-63.
Jeyaseelam E.C.,Tharmila,S.and Thavaranjit,A.C. (2011). Preliminary screening of alternative culture media for the growth of some selected fungi, Scholar Research library. 3 (3):389-393.
Ogundana, S.K. and Odah. I. N. (1984). Growth and sporulation of some fungi in local food stuff waste. Nigerian Food Journal, 2:141-144.
Rajasab, A.H. (2007). Parkia Powder Agar- a new medium for fungal culture. Current Science.92(10):1359-1360.
Ramalingam, S.T., Pereira, S and Pereira, C.T. (2007).Modern Biology for secondary school. Revised ed. Africana first publishers limited, Onitsha, Nigeria. 410 Pp.
Rinaldi, M.G. (1982). Use of potato flakes agar in clinical mycology. Journal of Clinical Microbiology, 15(1); 1159-1160.
https://doi.org/10.1128/jcm.15.6.1159-1160.1982
Samson,R.A., Hoekstra, E.S., Frisvad, J.C. and Filtenborg, O. (1996). Introduction to food- borne fungi. 5th ed. Baarn, Central Bureau voorshimmel cultures. 322Pp.
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