In vitro Activities of 6 Antimicrobial Agents against Bacterial Isolates from Cases of Neonatal Meningitis in Kano, Nigeria
DOI:
https://doi.org/10.47430/ujmr.1721.033Keywords:
Prevalence, aetiologic agents, neonates, meningitis antibiotics, resistanceAbstract
Meningitis is inflammation of meninges, which affects all age groups from the newborn to elderly and occurs more commonly during the first. It is usually caused by viral, bacterial or fungal pathogens. The objective of the study was to determine the antimicrobial susceptibility of bacterial isolates from cases neonatal meningitis at Murtala Muhammad Specialist Hospital (MMSH), Kano, Nigeria. Sixty neonates with suspected meningitis were enrolled of whom 10 were excluded due to contraindications to lumbar puncture (LP) or failed LP. Microscopy and culture were performed on all the fifty samples. The antibiotic susceptibility testing of the bacterial isolates from cases of neonatal meningitis to ampicillin (10µg), amoxycillin (25µg), ceftriaxone (30µg), ceftazidime (10µg), chlorampenicol (30µg) and gentamicin (10µg) was performed using the modified disc diffusion method (modified Kirby-Bauer technique). Bacteria were isolated in cerebrospinal of six neonates (12.0%), of which three isolates were Streptococcus pneumoniae, and the other 3 isolates were Escherichia coli, Pseudomonas aeruginosa, Klebsella pneumoniae and Acinetobacter. All isolated bacteria were resistant to ampicillin, amoxycillin, chlorampenicol and gentamicin but were sensitive to ceftriaxone and ceftazidime except for one Pseudomonas aeruginosa isolate which was resistant to ceftriaxone. It is recommended that neonates in Kano and its environment presenting with specific signs/symptoms of bacterial meningitis should be empirically treated with cephalosporins as first line therapy as confirmatory bacteriological tests are undertaken.
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References
Andargachew M, Afework K, Belay T (2005). Bacterial isolates from cerebrospinal fluids and their antibiotic susceptibility patterns in Gondar University Teaching Hospital, Northwest Ethiopia. Ethiop. J. Health Dev, 19(2): 161-164.
https://doi.org/10.4314/ejhd.v19i2.9986
Bell A.H, Brown D, Hallieday H L, Mcclure G, and McRied M. (1989). Meningitis in newborn 14 years review, Archive of diseases in childhood. 64:873-874.
https://doi.org/10.1136/adc.64.6.873
Clinical and Laboratory Standard Institute (2005). Performance Standard for antimicrobial susceptibility testing, 15th informational supplement, M100-515, Wayne, P: CLSI, 2005.
Clinical and Laboratory Standards Institute (2007). Performance Standards for Antimicrobial Disk Susceptibility Testing: seventeenth informational supplement. CLSI document M100-S17 [ISBN 1-56238-625-5]. Clinical and
Laboratory Standards Institute, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA. 27(1): 104-126.
Gebremariam A.( 1998). Neonatal meningitis in Addis Ababa: a 10-year review. Ann Trop paediatr, 18(4):279 - 83.
https://doi.org/10.1080/02724936.1998.11747960
Heath P T, NikYuoff N K, Baker C J. (2003). Neonatal meningitis, Pediatric Infectious Disease Unit. Arch Dis Child fetal neonatal, 88:173-178.
https://doi.org/10.1136/fn.88.3.F173
Jones ME, Draghi DC, Karlowsky JA, Sahm F, Badley JS (2004). Prevalence of antimicrobial resistance in bacteria isolated from central nervous system specimens as reported by US hospital laboratories from 200 to 2002. Ann Clin Microbial Antimicrobial, 3:3.
Laving A.M.R, Musoke R.N, Wasunna A.O. and Revathi G. (2003). Neonatal Bacterial Meningitis at the Newborn Unit of Kenyatta National Hospital; East African Medical Journal, 80(9): 456 - 462.
https://doi.org/10.4314/eamj.v80i9.8742
Luzia P.A.S, Laura G.C, Fernanda Q, Camila V. N, Rita L. (2007). Prevalence of newborn bacterial meningitis and sepsis during the pregnancy period for public health care system participants in Salvador, Bahia, Brazil. Braz J Infect Dis, 11(2): 1413-8670.
https://doi.org/10.1590/S1413-86702007000200021
Melese A. (2011). A Retrospective Study on the prevalence, etiological agents and associated risk factors for neonatal meningitis infection for the last 10 years (2001-2010) at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia. AAU- ETD. 4:19-32.
Morena E, Prats G, Sabata M, Perez T, Johnson JR, Andrew A (2006) Quionolone, Fluroquinolone and trimethoparim/sulfanethoxaosole resistance in relation to virulence determinants and phylogenetic background among uropathogenic Escherchia coli. J. Antimicrobial chemother, 57:204-211.
https://doi.org/10.1093/jac/dki468
Roberton N.R.C. (2012). Infection in the newborn. In: Roberton NRC, ed. Textbook of neonatology, 5thedn. Singapore, Churchill Livingstone, 39: 1013-1064.
Saez Llorens X, McCracken GH (2003). Bacterial meningitis in children. Lancet, 361:2139-2148.
https://doi.org/10.1016/S0140-6736(03)13693-8
Thaver D, Zaidi AK. (2009). Burden of neonatal infections in developing countries: a review of evidence from community-based studies. Pediatr Infect Dis J. 28(1): 3-9
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