Antibacterial Activities and Phytochemical Screening of Extracts of Flower of Azadiracthta indica (Neem) against Some Selected Clinical Isolates
DOI:
https://doi.org/10.47430/ujmr.1611.009Keywords:
Phytochemistry, Azadirachta indica, extracts, Clinical isolates, MIC, MBCAbstract
A study on the phytochemistry and antibacterial effects of petroleum ether, chloroform and methanol extract of flower of Azadirachta indica on five clinical isolates viz: Proteus mirabilis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae was carried out using deep well agar diffusion and broth dilution methods. The results obtained showed that methanol flower extract possessed the highest antibacterial activity against P. miravilis with inhibition zone of 23mm at the concentration of 4000μg/ml. The minimum inhibitory concentration (MIC) of the petroleum ether flower extract was 62.5μg/ml for all the test organisms while P. miravilis, S. aureus and K. pneumoniae had MIC value 62.5μg/ml with the exception of E. coli and P. aeruginosa.Methanol flower extract recorded 62.5μg/ml against P. miravilis, S. aureus. While E. coli, P. aeruginosa and K. pneumoniae had no MIC value across all the concentrations. the minimum bactericidal concentration (MBC) for the petroleum ether extracts was 125μg/ml, for chloroform extracts was 250μg/ml and for methanol extracts was 62.5μg/ml. Phytochemical analysis of the flower extracts showed the presence of reducing sugars, alkaloids, tannins, flavonoids, resins, and saponins, in all the extracts. Petroleum ether flower extract (PFE) showed antibacterial activities against all the test organisms while Methanol flower extract (MFE) had antibacterial activities against only two test organisms.
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References
Ahmad I, Memood, Z. and Mohammad, F. (1998): Screening of some Indian medicinal plants for their antimicrobial properties. Journal of Ethnopharmacol. 62:183-193.
https://doi.org/10.1016/S0378-8741(98)00055-5
Alade, P.I., and Irobi, O.N. (1993): Antimicrobial activities of crude leaf extracts of Acalyphawilkensian. Journal of Ethnopharmacology 39, 171-174.
https://doi.org/10.1016/0378-8741(93)90033-2
Baker C.N and Thormsberg C.H (1983): Inoculum standardization in antimicrobial susceptibility tests: Evaluation of overnight age culture. Journal of Clinical Microbiology, 17: 140-457.
https://doi.org/10.1128/jcm.17.3.450-457.1983
Biswas, K.I; Chattopadhyay, A and Banerijee Y,A. (2002): Biological activities and Medicinal properties of neem (Azadirachtaindica). Bandopadhyay, U, Curr Sci., 82:1336-1345.
Brain, K.R. and Tuner, T.D. (1975):The practical evaluation of phytopharmacheuticals. Wright Scientectica Publishers, Bristol.Pp57-58.
Boakye-Yiadom K, Fiagbe, N and Ayim ,S. (1987): Antimicrobial properties of some West African medicinal plants
IV. Antimicrobial of xylopic acid and constituents of the fruits of Xylopia athiopica (Anuonaceae). Lloydia40: 543-545.
Chattopadhyay R.R, Chattopadhyay R.N., and Maitra, S, K .(1993).Possible mechanism of anti-inflammatory activity of Azadirachtaindicaleaf extract.Indian J. Pharmacol. 25(2):99-100.
Chattopadhyay, R.R. (1996). Possible mechanism of anti-inflammatory activity of Azadirachtaindicaleaf extract: Part IV. Gen. Pharmacol. 27(3):431-434.
https://doi.org/10.1016/0306-3623(95)02070-5
Chattopadhyay,R.R, and Bandyopadhyay M (2005). Effect of Azadirachtaindicaleaf extract on serum lipid profile changes in normal and streptozotocin induced diabetic rats. African Journal of Biomedicine. Res. 8:101-104.
https://doi.org/10.4314/ajbr.v8i2.35769
Cheesborough, M. (2002): Biochemical tests to identify bacteria in laboratory practice in tropical countries. Cheesborough M. (ed).Cambridge Edition. pp. 63-87.
Cheesborough, M. (2012): Summary of the Clinical and Laboratory features of microorganisms. Cheesbrough M.(ed).cambridge edition pp.157- 233.
Ciulie, I. (1984):Methadology for the analysis of vegetables and drugs. Chemical Industry Division, UNIDO Romania. Pp57-58.
Clinical and Laboratory Standards Institute (2006): Methods for Dilution El- Mahmoodet al.,1421 of Antimicrobial Susceptibility Tests for bacteria that grow aerobically.Approved Standards,7thedn MG7-A7
Cunha, B.A. (2001):Antibiotic side effects. Med. Clinics North Am. 85:149-185.
https://doi.org/10.1016/S0025-7125(05)70309-6
Davis, J. (1994): Inactivation of antibiotic and the dissemination of resistance genes. Science 264, 375-382.
https://doi.org/10.1126/science.8153624
De, N and Ifeoma, E. (2002): Antimicrobial effect of components of the bark extracts of the neem (Azadirachtaindica A. juss). J. Technol.Dev.,8:23-28.
Diamond,R.D. (1993): The growing problem of mycoses in patients infested with human immunodeficiency virus. Review of Infectious Diseases 13, 480-486.
https://doi.org/10.1093/clinids/13.3.480
EL-Mahmood, A.M, Doughari J.H, and Ladan, N. (2008): Antimicrobial screening of stem bark extracts ofVitellariaparadoxa against some enteric pathogenic microorganisms. African Journal of Pharmacology.2(5): 089-094.
El Olemy, M.M; AL-Muhtadi, F.J.andAfif, (1994):Experimental Phytochemistry. A laboratory Manual. King Saud University press.8-9
Ergene, A.Guler, P.Tan, S; Mirici, S; Hamzaoglu, E. and Duran, A. (2006): Antibacterial and Antifungal Activity of Heracleum spondylium subsp. Artvinense.African Journal ofBiotechology. 5(11): 1087-1089.
Evans, M.W. (1995): Textbook on Pharmacognocy. 13th Edition.Bailiere-Tindal,London.
Ganguli, S. (2002). "Neem: A therapeutic for all seasons". Current Science 82(11):1304.
Harborne, J. B. (1973):Phytochemical Methods: A guide to Modern Techniques of Plant Analysis. 1stedition. Chapman and Hall Ltd. London.160
Hassain-Eshrat, H.M.A. (2002): Hypoglycaemic, hypolipidemic and antioxidant properties of curcumin from Curcuma longa, Linn. And partially purified product from Abroma auguta,Linn, in streptozotocin induced diabetes. Indian J. Clin. Biochem. 17(2): 33-43
https://doi.org/10.1007/BF02867969
Hugo, W.B; Russell A.D. (1983):Pharmaceutical Micrbiology 3rd Edition.Blackwell scientific publications. pp. 140-163.
Ketkar,A.Y and Ketkar CM (1995): Various uses of neem products: Medicinal uses including pharmacology in Asia, in H. Schmutterer (Ed). pp. 518-525.
https://doi.org/10.1002/3527603980.ch6
Mulligen, M.E., Murry-Leisure, K.A., Ribner, B.S., Standiford, H.C., John, J.F., Karvick, J.A., Kauffman, C.A., Yu, V.L., (1993): Methicillin resistant Staphylococcus aureus.
Natarajan, V; Veugopal, P.V and Menon, T. (2003): Effect of Azadirachtaindica(neem) on the growth pattern of dermatophytes. Indian J. Med.Microbiol., 21: 98-101.
https://doi.org/10.1016/S0255-0857(21)03129-7
Odugbemi, T. (2006). Medicinal Plants as Antimicrobials In: Outline and pictures of medicinal plants from Nigeria. University of Lagos Press. pp. 53-64
Ogbonnia, S.O, Enwuru N.V, Onyemenen E.U, Oyedele G.A, and Enwuru, C.A. (2008): Phytochemical evaluation and antibacterial profile of TreculiaAfricanaDecne bark extract on gastrointestinal bacterial pathogrns.mAfr. J. Biotechnol., 7(10): 1385-1389.
Piddock, K.J.V and Wise, R. (1989): Mechanisms of resistance to quinolones and clinical perspective. Journal of Antimicrobial Chemotherapy 23, 475-483
https://doi.org/10.1093/jac/23.4.475
Rao DVR, Sing K, Chopra, P, Chabra P, and Ramanujahi G (1986):In-vitro bactericidal activity of neem oil. Indian J. Med. Res., 84: 314-316
Rinaldi, M.G. (1991): Problems in the diagnosis of invasive fungal diseases. Review of Infectious Diseases 13, 493-495.
https://doi.org/10.1093/clinids/13.3.493
Robin, E.H ; Anril, W; Alexander, M; Loeto, M and Keith, K. (1998): Nasopharyngeal carriage and antimicrobial resistance in isolates of Streptococcus pneumoniae and Heamophilus influenzaeType b in children under 5 years of age in Botswana. International Journal of Infectious Diseases 3 (1), 18-25.
https://doi.org/10.1016/S1201-9712(98)90090-X
Saham, D.F, and Washington D.A. (1990): Antimicrobial susceptibility test dilution methods: In manuals of clinical of microbiology Lennette E. H. 5th edition, America-Society for Microbiology Washington D. C, pp. 1105-1106.
Singh, M; Chaudhry, M.A; Yadava, J.N.S; and Sanyal, S.C.(1992): The spectrum of antibiotic resistance in human and veterinary isolates of Escherichia coli collected from 1984-1986 in Northern India. Journal of Antimicrobial Chemotherapy 29, 159-168.
https://doi.org/10.1093/jac/29.2.159
Sofowora, S.A. (1984):Medicinal plants and Traditional Medicine in Africa. Spectrum books Ltd book ltd. 1st edition. 150-151 and 162-172.
Sofowora, A. (1993): Medicinal plants and Traditional Medicine in Africa. Spectrum book Limited, Ibadan, Nigeria.Pp385-388.
Subapriya, R. and Nagini S. (2005): Medicinal properties of neem leaves: areview. Curr Med Chem Anticancer Agents., 5(2):149-6.
https://doi.org/10.2174/1568011053174828
Vermani, K. andGarg S (2002).Herbal Medicines for Sexually Transmitted Diseases and AIDS.Journal of Ethnopharmacology. 80:49-66.
https://doi.org/10.1016/S0378-8741(02)00009-0
WHO (1993): Summary of WHO guidelines for the assessment of herbal medicines. Herbal Gram 28:13-14.
WHO (2003): Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health importance in developing world Genera. pp. 103-322.
Yaqoup, S.O. Shami, E.A, Ahmed, B. and Asha, Z.E.(2007): Antimicrobial activity of some medicinal plants against some Gram positive, Gram negative and fungi. Department of Molecular Biology, EL Neelain University, Sudan. www.astf.net Accessed on 20th August 2009
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