Evaluation of the Performance of Widal Slide Agglutination Test Compared to Blood Culture and Evaluation of Interferon Gamma Response in the Diagnosis of Typhoid Fever

Authors

  • Mujahid, N.S. Department of Microbiology, Kano University of Science and Technology Wudil
  • Mohammed, Y. Department of Medical Microbiology and Parasitology, Bayero University Kano
  • Yusuf, I. Department of Pathology, Faculty of Clinical Sciences Bayero University Kano
  • Sani, N.M. Department of Microbiology and Biotechnology, Federal University Dutse
  • Abdulkadir, B. Department of Microbiology, Umaru Musa Yar’adua University Katsina
  • Nasiru, M.S Department of Medical Microbiology, Aminu Kano Teaching Hospital Kano
  • Wada, M. Department of Microbiology and Biotechnology, Federal University Dutse

DOI:

https://doi.org/10.47430/ujmr.2052.016

Keywords:

Typhoid, Widal test, Blood culture, Interferon gamma

Abstract

Typhoid fever remains a public health challenge in developing countries including Nigeria. Widal test is widely used for the diagnosis of typhoid fever due to its simplicity and short turnaround time. However, the specificity of this test has been debated. The aim of the study was to evaluate the performance of Widal test compared to blood culture and determine interferon gamma response among the study subjects. Blood samples were collected from 90 patients who complained of fever and other symptoms suggestive of typhoid fever. Widal slide agglutination test, automated blood culture and interferon gamma concentrations were conducted using rapid antibody detection kit, BACTEC and sandwich enzyme linked immunosorbent assay (ELISA) respectively. Of the 90 samples tested, 63 (70.0%) were positive for anti-Typhi O antigen while 42 (46.7%) were positive for anti-Typhi H antigen. Similarly, 18 (20%) of the blood samples were non- S. Typhi culture positive while 72 (80%) had no bacteria isolated. None of the cases had S. Typhi positive culture. With regards to interferon gamma, subjects with lower levels of 15pg/mL had no bacteria isolated from their blood. As the interferon gamma concentration increased, more subjects had non- S. Typhi bacteria isolated from their blood which shows the relationship between interferon gamma and bacteraemia. The study demonstrated that the use of Widal serology test in the diagnosis of typhoid fever may be erroneous as all the samples were found to be negative for S. Typhi using the gold standard culture methods while Interferon gamma concentration was statistically related to the isolation of non- S. Typhi in blood culture as such, could be a good marker for the development of an alternative screening test, possibly an interferon gamma based detection system for typhoid fever. However, further research is recommended to elucidate that.

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References

Abbas, H. H., Ali, K. H., Ibrahem, D. M., Ammar, F., Abd-alwahed, M. T., and Hafedh, H. (2014). Comparison study between typhoid fever and tuberculosis patients to induce and produce of Th-1 Journal of Experimental Biology, 4 (3):20-26.

Abdulkarim, I. A., and Mohammed, M. U. (2017).Spatio-temporal trends of typhoid fever among youths attending Muhammad Abdullahi Wase Specialist cytokines (IL-18 and IFN- γ). Europian 121 Hospital in Kano Metropolis, Nigeria. Bayero Journal of Pure and Applied Sciences, 10 (2): 115.

https://doi.org/10.4314/bajopas.v10i2.20

Ajibola, O., Mshelia, M. B., Gulumbe, B. H. and Eze, A. A. (2018). Typhoid Fever Diagnosis in Endemic Countries: A Clog in the Wheel of Progress? Medicina (Kaunas, Lithuania), 54 (2): 1-12.

https://doi.org/10.3390/medicina54020023

Akinyemi, K. O., Oyefolu, A. O. B., Mutiu, W. B., Iwalokun, B. A., Ayeni, E. S., Ajose,S. O., and Obaro, S. K. (2018). Typhoid fever: Tracking the trend in Nigeria. American Journal of Tropical Medicine and Hygine, 99 (3): 41-47.

https://doi.org/10.4269/ajtmh.18-0045

Andualem, G., Abebe, T., Kebede, N., Gebre- Selassie, S., Mihret A. and Alemayehu,H. (2014).A comparative study of Widal test with blood culture in the diagnosis of typhoid fever in febrile patients.BMC Research Notes, 7 (653): 1-6.

https://doi.org/10.1186/1756-0500-7-653

Anvarinejad, M., Pouladfar, G. R., Pourabbas, B., Amin Shahidi, M., Rafaatpour, N., Dehyadegari, M. A. (2016). Detection of Salmonella spp. with the BACTEC 9240 Automated Blood Culture System in 2008 - 2014 in Southern Iran (Shiraz): Biogrouping, MIC, and Antimicrobial Susceptibility Profiles of Isolates. Jundishapur Journal of Microbiology, 9 (4): 1-6.

https://doi.org/10.5812/jjm.26505

Bhatti, A. B., Ali, F., and Satti, S. A. (2015). Cross-Reactivity of Rapid Salmonella Typhi IgM Immunoassay in Dengue Fever without Co-Existing Infection. Cureus, 7 (12):1-9.

https://doi.org/10.7759/cureus.396

Chalya, P. L., Mabula, J. B., Koy, M., Kataraihya, J. B., Jaka, H., Mshana, S. E. (2012). Typhoid intestinal perforations at a University teaching hospital in Northwestern Tanzania: A surgical experience of 104 cases in a resource-limited setting. World Journal of Emergency Surgery, 7 (1): 4.

https://doi.org/10.1186/1749-7922-7-4

Chu, C., Anh, D. D., Son, N. H., Canh, D. G., Lin, F.-Y. C., Thiem, V. D., … Mao, N. D. (2011). The Vi Conjugate Typhoid Vaccine Is Safe, Elicits Protective Levels of IgG Anti-Vi, and Is Compatible with Routine Infant Vaccines. Clinical.Vaccine and Immunology, 18 (5), 730-735.

https://doi.org/10.1128/CVI.00532-10

Deksissa, T., and Gebremedhin, E. Z. (2019). A cross-sectional study of enteric fever among febrile patients at Ambo hospital: prevalence, risk factors, comparison of Widal test and stool culture and antimicrobials susceptibility pattern of isolates. BMC Infectious Diseases, 19 (288): 1-12.

https://doi.org/10.1186/s12879-019-3917-3

Fidan, I., Yesilyurt, E., Gurelik, F. C., Erdal, B., and Imir, T. (2008). Effects of recombinant interferon-γ on cytokine secretion from monocyte-derived macrophages infected with Salmonella typhi. Comparative Immunology, Microbiology and Infectious Diseases, 31 (6): 467-475.

https://doi.org/10.1016/j.cimid.2007.07.014

Gberikon, G. M., Olabode, V. B., and Ogbonna,I. O. (2019). Determination of Antibiotic Resistance of Salmonella and Molecular Confirmation of Drug Resistance Salmonella Species Isolated From Raw Chicken and Quail Eggs from Selected Farms in Jos ,International Journal of Innovative Research and Development, 8 (1): 37-42.

https://doi.org/10.24940/ijird/2019/v8/i1/JAN19012

Igiri, B. E., P.C., I., G.C., E., Jimoh, O., M.A.,S., and G.O, I. (2018). Diagnostic investigations and prevalence of enteric fever in Ahmadu Bello University Teaching Hospital Shika-Zaria ,. Clinical Microbiology and Infectious Diseases, 3 (1): 1-5.

https://doi.org/10.15761/CMID.1000131

Nas, F. ., Ali, M., and Yahaya, A. (2017). Malaria and Typhoid Fever Co-Infection Co Infection Among Febrile Patients in. Bayero Journal of Pure and Applied Sciences, 10 (2): 122-125.

https://doi.org/10.4314/bajopas.v10i2.21

Pham, O. H., and McSorley, S. J. (2015).Protective host immune responses to Salmonella infection.Future Microbiology, 10 (1):101-110.

https://doi.org/10.2217/fmb.14.98

Siegel, S. A. R., Cavanaugh, M., Ku, J. H., Kawamura, L. M., and Winthrop, K. L. (2018). Specificity of QuantiFERON-TB Plus, a New-Generation Interferon Gamma Release Assay. Journal of Clincal Microbiology, 56 (12):629-618.

https://doi.org/10.1128/JCM.00629-18

World Health Organization.(2018). WHO | Typhoid. Retrieved March 23, 2019, from https://www.who.int/mediacentre/fac tsheets/typhoid/en/

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Published

30-12-2020

How to Cite

Mujahid, N.S., Mohammed, Y., Yusuf, I., Sani, N.M., Abdulkadir, B., Nasiru, M.S, & Wada, M. (2020). Evaluation of the Performance of Widal Slide Agglutination Test Compared to Blood Culture and Evaluation of Interferon Gamma Response in the Diagnosis of Typhoid Fever. UMYU Journal of Microbiology Research (UJMR), 5(2), 117–122. https://doi.org/10.47430/ujmr.2052.016

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