Phytochemical Screening and Antibacterial Activity of Mangifera indica Extracts
DOI:
https://doi.org/10.47430/ujmr.1611.004Keywords:
Antibacterial activity, agar well diffusion, phytochemical tests, sensitivity and zone of inhibitionAbstract
Mangifera indica leaves are used for the treatments of various ailments in folklore medicine. This research was aimed to determine phytochemical composition and antibacterial activity of leaves extracts of Mangifera indica. Powdered leaves of Mangifera indica were extracted with water, ethanol and chloroform solvents via percolation method. The extracts were tested for antibacterial activity against clinical isolates of Salmonella Typhi, Salmonella Paratyphi A and Salmonella Paratyphi B using agarwell diffusion method. The extracts were further subjected to phytochemical tests for the presence of secondary metabolites using standard procedures. Results of the sensitivity test showed that highest zone of inhibition was observed in ethanolic extract with 13mm for S. Paratyphi A, 11mm for S. Paratyphi B, and 10mm for S. Typhi, followed by aqueous extract with 11mm for S. Paratyphi B and 10mm for S. Typhi. S. Paratyphi A, was resistant to both aqueous and chloroform extract of M. indica while S. Paratyphi B was onlyresistant to chloroform extract. Results of phytochemical screening indicated the presence of alkaloids, flavonoids, steroids, tannins and phenols.
Downloads
References
Aliyu, B. S. (2006): Some ethno-medicinal plants of the Savannah Regions of West Africa Description and phytochemicals. Triumph publishing company. Pp 135-152.
Ayoola, G.A., Coker, H.A.B., Adesegun, S.A., Adepoju-Bello, A.A., Obaweya, K., Ezennia, E.C. and Atangbayila, T.O. (2008). Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in SouthwesternNigeria. Tropical Journal of Pharmaceutical Research,7, 1019-1024.
https://doi.org/10.4314/tjpr.v7i3.14686
Brain, K.R. and Turner, T.D. (1975): The practical evaluation of phytochemicals.Wright Scientechina, Bristol: Pp.57 - 58.
Cheesbrough, M. (2006): Biochemical tests to identify bacteria. District Laboratory practice in Tropical Countries. Cheesbrough M (ed.), Cambridge, Part II: Pp 63-90.
https://doi.org/10.1017/CBO9780511543470
Ciulci, I. (1994): Methodology for the analysis of vegetable drugs. Chemical industries branch, Division of industrial operations. UNIDO, Romania: Pp.24 - 67.
https://doi.org/10.1007/978-1-349-13185-3_14
Dahiru, D., Malgwi, A. R. and Sambo, S. H. (2013): Growth Inhibitory Effect of Sennasiamea Leaves Extracts on Selected Microorganisms. American Journal of Medicine and Medical Sciences,3(5): 103-107.
Duraipandiyan, V., M., Ayyanar L. and Ignacimuthu S. (2006): Antimicrobial activity of some ethnomedicinal plants. Asian Journal of Microbiology; 5: 334-337.
Dweck, A.C. (2001): Article for cosmetics and toiletries magazine ethnobotanical plants from Africa. Black Medicare Ltd, Itshire, UK.
Fatope, A., O., Ibrahim, H. and Takeda, Y. (1993): Screening of higher plants reputed as pesticides using brine shrimp lethality bioassay. International Journal of Pharmacognosy,31: 250-256.
https://doi.org/10.3109/13880209309082949
Harborne, J. B. and Williams, C.A. (2000): Advances in flavonoid research since 1992. Phytochemistry Journal; 55: 481-504.
https://doi.org/10.1016/S0031-9422(00)00235-1
National Committee for Clinical Laboratory Standards (NCCLS, 2008): Performance standards for Antimicrobial Susceptibility Testing: Ninth Informational Supplement. NCCLS document M100-S9. National Committee for Clinical Laboratory Standards, Wayne, PA.
Odunbaku, O. A. and Ashidi, J.S. (2012):Antimicrobial effect of ethanol leaf extracts of selected medicinal plants on some human pathogenic microbes. International Journal of Science and Advanced Technology;2:8.
Pintu, K. D. and Arna, P. (2014):Effects of aqueous young leaves extract of Mangifera indicaon gm (-) microorganisms causing gastro- intestinal disorders. Asian Journal of Plant Science and Research;4(1): 23- 27.
Pritesh, R. D. and Zara S. (2015):Preliminary studies on phytochemicals and cytotoxicactivity of methanolic rhizome extract of Hedychiumcoronarium. Journal of Pharmacognosy and Phytochemistry; 4(1): 136-139
Sandhya, B., Thomas, S., Isabel, W. and Shenbagarathai, R. (2006): Ethnomedicinal plants used by the Valaiyan community of Piranmalai hills (reserved forest).Journal of Complementary and alternative medicines,3: 101-114.
https://doi.org/10.4314/ajtcam.v3i1.31145
Shah, K., Patel, M., Patel, R. and Parmar, R. (2010): Pharmacognosy Review, 4:42-48.
https://doi.org/10.4103/0973-7847.65325
Shanjida, I., T., Hossain, M. I. and Tasneema, I. (2015): Antimicrobial activities of Psidium guajava, Carica papaya and Mangifera indica against some grampositive and gram negative bacteria. Journal of Pharmacognosy and Phytochemistry3(6): 125-129.
Sofowara, A. (1993): Medicinal plants and traditional medicine in Africa. Spectrum Books. Nigeria. 2nd Ed. Pp 10-158.
Wauters, G., Charlier, J. and Janssens, M. (1995): Agglutination of pYV + Yersinia enterocolitica strains by agglutinin from Mangifera indica. Journal of Clinical Microbiology,33:772-774.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 UMYU Journal of Microbiology Research (UJMR)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
