Antibacterial Potential of Endophytic Fungi Isolated from Psidium guajava (Guava) Leaf against Escherichia coli and Klebsiella pneumoniae

Authors

  • Hussaini, I. M. Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University Zaria, Nigeria https://orcid.org/0000-0003-1230-248X
  • Ibrahim, S. Department of Microbiology, Faculty of Life Sciences, AhmaduBello University Zaria, Nigeria
  • Usman, A. Department of Microbiology, Faculty of Science, Kaduna State University Kaduna, Nigeria
  • Musa, B. Department of Microbiology, Faculty of Life Sciences, AhmaduBello University Zaria, Nigeria https://orcid.org/0000-0001-6672-195X

DOI:

https://doi.org/10.47430/ujmr.2493.021

Keywords:

Endophytes, fungi, antibacterial, Escherichia coli, Klebsiella pneumoniae

Abstract

Study’s Novelty/Excerpt

  • This study investigates the antibacterial potential of endophytic fungi associated with Psidium guajava leaves against Escherichia coli and Klebsiella pneumoniae, revealing novel antimicrobial properties.
  • Through isolation and identification of eight endophytic fungi, four were found to exhibit significant antibacterial activity.
  • This work highlights the potential of endophytic fungi from P. guajava as a promising source of new antibacterial agents, contributing to the ongoing search for solutions to antimicrobial resistance.

Full Abstract

Antimicrobial resistance has been recognized as a major issue of public health concern and it remains a global threat of the health care system. Endophytic fungi associated with medicinal plants are reported as promising reservoir of novel antibiotics. The study aim was to determine the antibacterial potential of endophytic fungi associated with Psidium gaujava leaf against Escherichia coli and Klebsiella pneumoniae. Leaves of P. guajava were surface sterilized and inoculated on plates of Potato Dextrose Agar and incubated at room temperature. Endophytic fungal isolates that emerged were identified using their macroscopic (cultural) and microscopic characteristics. The endophytes were screen for antibacterial activity on E. coli and K. pneumoniae isolates. Antibacterial activity of endophytic fungi ethyl acetate extracts with antibacterial activity was also evaluated against isolates of E. coli and K. pneumoniae by agar well diffusion technique. Minimum Inhibitory Concentrations and Minimum Bactericidal Concentrations of the ethyl acetate extracts were also determined. Eight endophytic fungi were isolated from P. guajava leaves and four had antibacterial activity namely: Gonatobotrys sp. P21, Xylaria sp. P11, Fusarium sp. P22 and Trichoderma sp. P25. The extracts exhibited antibacterial activity with zones of inhibition ranging from 11.5 ± 0.5mm to 18.5 ± 1.5mm for E. coli and 12.5 ± 0.5mm to 21.0 ±1.0mm for K. pneumoniae. The MIC was found to be 500mg/mL and 250mg/mL for E. coli and K. pneumoniae respectively. Endophytic fungi isolated from P. gaujava leaf are potential source of novel antibacterial drug since they possess antibacterial activity against isolates of E. coli and K. pneumoniae.

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References

Biswas, B., Rogers, K., McLaughlin, F., Daniels, D., Yadav, A. (2013).Antimicrobial activities of leaf extracts of Guava (Psidiumguajava L.) on two gram-negative and gram-positive bacteria. International Journal of Microbiology, 746165. https://doi.org/10.1155/2013/746165

Carbungco, E.S., Pedroche, N.B., Panes, V.A. and De la Cruz, T.E. (2017). Identification and characterization of endophytic fungi associated with the leaves of Moringaoleifera Lam. ActaHorticulturae,1158, 373-380 https://doi.org/10.17660/ActaHortic.2017.1158.42

Digra, S. and Nonzom, S. (2023). An insight into endophytic antimicrobial compounds: An updated analysis. Plant Biotechnology Report, 1–31. https://doi.org/10.1007/s11816-023-00824-x

Gouda, S., Das, G., Sen, S.K., Shin, H.S. and Patra, J.K. (2016). Endophytes: A Treasure House of Bioactive Compounds of Medicinal Importance. Frontier in Microbiology, 7:1538. https://doi.org/10.3389/fmicb.2016.01538

Gupta, A., Meshram, V., Gupta, M., Goyal, S., Qureshi, K.A., Jaremko, M. and Shukla, K.K. (2023). Fungal Endophytes: Microfactories of Novel Bioactive Compounds with Therapeutic Interventions; A Comprehensive Review on the Biotechnological Developments in the Field of Fungal Endophytic Biology over the Last Decade. Biomolecules, 13, 1038.https://doi.org/10.3390/biom13071038

Hilario, F., Vanessa , M., Chapla, A. R., Araujo., Paulo T., Sano,T., Bauaba, M. and Lourdes, C.S. (2017). Antimicrobial Screening of Endophytic Fungi Isolated from the Aerial Parts of Paepalanthuschiquitensis (Eriocaulaceae) Led to the Isolation of Secondary Metabolites Produced by Fusariumfujikuroi, Journal of the Brazilian Chemical Society, 28(8): 1389-1395. https://doi.org/10.21577/0103-5053.20160312

Hussaini, I.M., Ahmed, H.S., Ahmad, H., Sulaiman, M.A. and Usman, A. (2022). Preliminary Screening for Antibacterial Activity of Endophytic Fungi isolated from Azadirachtaindica and MenthapiperitaPhyllosphere against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Algerian Journal of Biosciences, 03(02): 056–060. https://doi.org/10.57056/ajb.v3i2.57

Kaneria, M. and Chanda, S. (2011). Phytochemical and PharmacognosticEvalution of leaves of Psidiumguajava L. (Myrtaceae), Pharmacognosy Journal, 3(23): 41 - 45. https://doi.org/10.5530/pj.2011.23.6

López-Pérez PM, Grimsey E, Bourne L, Mikut R and Hilpert K (2017).Screening and Optimizing Antimicrobial Peptides by Using SPOT-Synthesis.Frontiers in Chemistry, 5:25. https://doi.org/10.3389/fchem.2017.00025

Marcellano, J.P., Collanto, A.S. and Fuentes, R.G. (2017).Antibacterial Activity of Endophytic Fungi Isolated from the Bark of Cinnamomummercadoi.Pharmacogn J, 9(3):405-9. https://doi.org/10.5530/pj.2017.3.69

Meshram, V., Elazar, M., Maymon, M., Sharma, G., Shawahna, R., Charuvi, D. and Freeman, S. (2023). EndophyticFusariumclavum confers growth and salt tolerance in Cucumismelo. Environmental and Experimental Botany, 206: 105–153. https://doi.org/10.1016/j.envexpbot.2022.105153

O’Neill, J. (2016). Tackling Drug-Resistant Infections Globally: Final Report and Recommendations Review on Antimicrobial Resistance.

Santiago, S., Sun, L., Munro, M.H.G. and Santhanam, J. (2014).Polyketide and benzopyran compounds of an endophytic fungus isolated from Cinnamomummollissimum: biological activity and structure. Asian Pacific Journal of Tropical Biomedicine, 4(8): 627-632. https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0030

Savitha, D., Rita, M., Sonam, V., Varuni, G., Kirti, P. and Basavaraj, H. (2012). Phytochemical analysis, antimicrobial and antitumour screening of endophytes of TinosporacordifoliaInternational Journal of Pharmaceutical and Biological Science,3(4): 533 – 540

Susilawati, Amalia, E., Oktariana, D. and Khairunnisa, M.S. (2018). Antibacterial Activity of Endophytic Fungi Isolated from the Leaves of JambuBiji (Psidiumguajava L.) Journal of Physics: Conference Series 1095 (2018) 012041. https://doi.org/10.1088/1742-6596/1095/1/012041

Tchamgoue, E.N., Fanche, S.A.Y., Ndjakou, B.L., Matei, F. andNyegue, M.A. (2020). Diversity of Endophytic Fungi of Psidiumguajava (Myrtaceae) and Their Antagonistic Activity against Two Banana Pathogens.20(11): 86-101, https://doi.org/10.9734/JAMB/2020/v20i1130304

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Published

29-06-2024

How to Cite

Hussaini, I. M., Ibrahim, S., Usman, A., & Musa, B. (2024). Antibacterial Potential of Endophytic Fungi Isolated from Psidium guajava (Guava) Leaf against Escherichia coli and Klebsiella pneumoniae. UMYU Journal of Microbiology Research (UJMR), 9(3), 180–186. https://doi.org/10.47430/ujmr.2493.021