Prevalence of Enterobacteriaceae from Clinical Isolates in Federal Teaching Hospital Gombe, Nigeria
DOI:
https://doi.org/10.47430/ujmr.2491.027Keywords:
Pathogens, Enterobacteriaceae, prevalence, clinical samples, identificationAbstract
Study’s Novelty/Excerpt
- This study investigates the prevalence of Enterobacteriaceae in clinical samples from the Federal Teaching Hospital Gombe, providing a comprehensive analysis of 420 non-duplicate isolates over a four-month period.
- The research uniquely identifies the distribution of various Enterobacteriaceae species, with Escherichia coli being the most prevalent, and correlates these findings with the types of clinical specimens, revealing urine as the most common source.
- This work underscores the necessity for further research into the antimicrobial resistance profiles of these isolates, aiming to inform more effective treatment strategies for bacterial infections in the region.
Full Abstract
Enterobacteriaceae is a family of Gram-negative, oxidase-negative, and catalase-positive bacteria mostly found in Humans and animals' intestines. Some of these organisms are enteric opportunistic pathogens associated with urinary tract infections, respiratory tract infections, and wound infections, whereas others are regularly pathogenic for humans. This study aimed to determine the prevalence of Enterobacteriaceae in clinical samples from Federal Teaching Hospital Gombe between August 2022 and November 2022. A total of 420 non-duplicate isolates from various clinical samples were analyzed in the study. The isolates were identified based on cultural characteristics, Gram staining, and standard biochemical tests. Out of the 420 isolates identified, Escherichia coli was the most prevalent with 163(38.8%) isolates, followed by Klebsiella pneumoniae with 69(16.4%), Klebsiella oxytoca with 61(14.5%), Proteus spp. 28(6.7%), Citrobacter spp. 27(6.4%), Enterobacter spp. 22(5.2%), Serratia marcescens 13(3.1%), Providencia spp. 12(2.9%), Yersinia enterocolitica 11(2.6%), Morganella morganii 3(0.7%) and Salmonella spp. with 2(0.5%). Based on clinical specimens, urine had the highest percentage of isolates with 53.4%, followed by wound swab (19.1%), stool (10.1%), High vaginal swab (6.8%), Endocervical swab (3.6%), sputum (3.1%), blood (1.4%), Cerebrospinal fluid (1.0%) and semen (1.0%) and then pleural fluid (0.5%). In conclusion, Enterobacteriaceae clinical isolates were highly prevalent in Federal Teaching Hospital Gombe. Further research to assess the antimicrobial resistance profile of these clinical bacterial organisms in the study area is recommended for effective treatment options for bacterial infections.
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References
Archibald, L. K., & Jarvis, W. R. (2011). Health care–associated infection outbreak investigations by the Centers for Disease Control and Prevention, 1946–2005. American journal of epidemiology, 174(suppl_11), S47-S64. https://doi.org/10.1093/aje/kwr310
Bhatia, R. & Ichhpujani, R. L. (Ed.) (2008). Essentials of Medical Microbiology Fourth Edition. Jaypee Brothers Medical Publishers (P) LTD, India. P. 222. https://doi.org/10.5005/jp/books/10281_39
Carroll, K. C., Butel, J. S., Morse, S. A. & Mietzner, T. (Ed.) (2016). Jawetz, Melnick & Adelberg's Medical Microbiology 27th Edition. Mc Graw-Hill Education, p. 231.
CDC, 2006: New Sudy Profiles Women's use of Healthcare, National Center for Health Statistics. Health, United States, 2006 With Chartbook on Trends in the Health of Americans. Hyattsville, MD: 2006.
Cheesbrough, M. (Ed.) (2006). District Laboratory Practice in Tropical Countries Second Edition. Cambridge University Press, New York, pp. 65-70. https://doi.org/10.1017/CBO9780511543470
Eltai, N. O., Al Thani, A. A., Al-Ansari, K., Deshmukh, A. S., Wehedy, E., Al-Hadidi, S. H., & Yassine, H. M. (2018). Molecular Characterization of Extended-spectrum β -lactamases Enterobacteriaceae Causing Lower Urinary Tract Infection among Pediatric Population. Antimicrobial Resistance & Infection Control, 7(1), 90. https://doi.org/10.1186/s13756-018-0381-6
Ferri, M., Ranucci, E., Romagnoli, P., & Giaccone, V. (2017). Antimicrobial resistance: A global emergingthreat to public health systems. Critical reviews in food science and nutrition, 57(13), 2857-2876. https://doi.org/10.1080/10408398.2015.1077192
Garba, L., Abdulfatah, A., Adamu, M. T., Ismail, H., Yusuf, I., & Dahiru, A. S. (2021a). Evaluation of Antibacterial Potency of Endophytic Fungi Isolated from Mentha piperita. International journal of Innovative Approaches in Science Research, 5(4), 251-262. https://doi.org/10.29329/ijiasr.2021.414.6
Garba, L., Muhammad, M. B., Adamu, M. T., Isa, S., Abdullahi, M. M., & Yarma, A. A. (2021b). Potential in vitro Inhibitory Effects of Moringa oleifera Leaf Extracts on Extended-spectrum β-Lactamase Producing Bacteria. Journal of Fundamental and Applied Sciences, 13(1), 137-150. http://dx.doi.org/10.4314/jfas.v13i1.8
Garba, L., Yushaâ, M., Abdullahi, M. M., Abubakar, M. U., Inuwa, A. B., Isa, S., & Adamu, M. T. (2018).Effectiveness of Double Discs Synergy Test in the Confirmation of Extended Spectrum β-lactamas e(ESβL) Production. Journal of Biochemistry, Microbiology and Biotechnology, 6(2), 15-18. https://doi.org/10.54987/jobimb.v6i2.428
Gill, A. A., Singh, S., Thapliyal, N., & Karpoormath, R. (2019). Nanomaterial-based optical and electrochemical techniques for detection of methicillin-resistant Staphylococcus aureus: a review. Microchimica Acta, 186, 1-19. https://doi.org/10.1007/s00604-018-3186-7
Gillespie, S. H. and Hawkey, P. M. (Ed.) (2006). Principles and Practice of Clinical Bacteriology Second Edition. John Wiley & Sons Ltd, The Atrium, Southern Gate, Chiichester, West Sussex PO19 8SQ, England, p. 341
Hamid, O. M., Gumaa, S. A., Ibrahim A. and Bayoumi, M. (2019). Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae: Hospital-Acquired Urinary Tract Infections, Khartoum-Sudan. Annals of Microbiology and Research, 3(1):76-82. https://doi.org/10.36959/958/572
Hapsari, A. P., Wahyuni, C. U., & Mudjianto, D. (2018). Knowledge of surveillance officers on identification of healthcare-associated infections in surabaya. Jurnal Berkala Epidemiologi, 6(2), 130. https://doi.org/10.20473/jbe.V6I22018.130-138
Janda, J. M., & Abbott, S. L. (2020). The Changing Face of the Family Enterobacteriaceae (Order: "Enterobacterales" ): New Members, Taxonomic Issues, Geographic Expansion, and New Diseases and Disease Syndromes. Clinical Microbiology Reviews, 34(2), e00174-20. https://doi.org/10.1128/CMR.00174-20
Michael, G., Garba, L., Adamu, M.T.,Manga, M.M.,Yarma, A.A. & Isa, S. (2023a).Detection of Multidrug-Resistant Acinetobacter baumannii among Gram-Negative Bacteria Isolated from Clinical Samples. International Journal of Innovative Approaches in Science Research, 7(4), 101-117. https://doi.org/10.29329/ijiasr.2023.629.2
Michael, G., Garba, L., Adamu, M.T., Saleh, Z.M., Isa, S. & Manga, M.M.(2023b). Identification of Multiple Drug-Resistance Genes in Clinical Isolates of Acinetobacter baumannii. Journal of Biochemistry, Microbiology and Biotechnology, 11(2), 61-65. https://doi.org/10.54987/jobimb.862
Mohamed, E. S., Khairy, R. M. M., & Abdelrahim, S. S. (2020). Prevalence and Molecular Characteristics of ESBL and AmpC β -lactamase Producing Enterobacteriaceae Strains Isolated from UTIs in Egypt. Antimicrobial Resistance & Infection Control, 9(1), 198. https://doi.org/10.1186/s13756-020-00856-w
Morfín-Otero, R., Mendoza-Olazarán, S., Silva-Sánchez, J., Rodríguez-Noriega, E., Laca-Díaz, J., Tinoco-Carrillo, P., Petersen, L., López, P., Reyna-Flores, F., Alcantar-Curiel, D., Garza-Ramos, U., & Garza-González, E. (2013). Characterization of Enterobacteriaceae Isolates Obtained from a Tertiary Care Hospital in Mexico, Which Produces Extended-Spectrum β-Lactamase. Microbial Drug Resistance, 19(5), 378–383. https://doi.org/10.1089/mdr.2012.0263
Nimer N. A. (2022). Nosocomial Infection and Antibiotic-Resistant Threat in the Middle East. Infection and Drug Resistance, 15: 631-639. https://doi.org/10.2147/IDR.S351755
Prasad, N. P., Maharjan, P., Joshi, G. & Raj, P. K. (2016). Emerging Perils of Extended-spectrum β-lactamase Producing Enterobacteriaceae Clinical Isolates in a Teaching Hospital of Nepal. Biomed Research International, volume 2016, ID 1782835: 1-7. https://doi.org/10.1155/2016/1782835
Saba N., Balwan W.K. (2023). Study of frequency, epidemiology of nosocomial infections in healthcare centres. Saudi Journal of Pathology and Microbiology, 8(7):160-6. https://doi.org/10.36348/sjpm.2023.v08i07.001
Teklu, D. S., Negeri, A. A., Legese, M. H., Bedada, T. L., Woldemariam, H. K., & Tullu, K. D. (2019). Extended-spectrum Beta-lactamase Production and Multi-drug Resistance among Enterobacteriaceae Isolated in Addis Ababa, Ethiopia. Antimicrobial Resistance & Infection Control, 8(1), 39. https://doi.org/10.1186/s13756-019-0488-4
Thenmozhi, S., Moorthy, K., Sureshkumar, B. T., & Suresh, M. (2014). Antibiotic Resistance Mechanism of ESBL Producing Enterobacteriaceae in Clinical Field: A Review. International Journal of Pure and Applied Bioscience, 2(3): 207-226 (2014).
Tolulope, A. A., Temitayo, A. A., Wumi, B. O. & Oladehinde, A. O. (2020). Prevalence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Gram-Negative Pathogens from Patients in a Tertiary Care Hospital in Nigeria. Journal of Medical Science and Clinical Research, 8(5) 01-10. https://doi.org/10.18535/jmscr/v8i5.01
Tarigan, G. E., Nawan, N., & Toemon, A. I. (2023). Identification and Resistance Testing of Bacteria Causing Nosocomial Infections in Surgery Inpatient Rooms. Disease Prevention and Public Health Journal, 17(1), 100-108. https://doi.org/10.12928/dpphj.v17i1.6875
Tula, M. Y. & Iyoha, O. (2020). Phenotypic Detection of Extended-spectrum β-lactamases (ESβLs) among Enterobacteriaceae and Pseudomonmas aeruginosa from Mubi, Adamawa State, Nigeria. Highland Medical Research Journal, 20(1): 10-15. https://doi.org/10.3126/tujm.v7i0.33801
Vaiyapuri, M., Joseph, T. C., Rao, B. M., Lalitha, K. V., & Prasad, M. M. (2019). Methicillin‐resistant Staphylococcus aureus in seafood: prevalence, laboratory detection, clonal nature, and control in seafood chain. Journal of food science, 84(12), 3341-3351. DOI: 10.1111/1750-3841.14915
Yang W, Yao H, Xi C, Ye X, Chen Q, Zhang J, Yu J, Hu J. (2023). Prevalence and Clinical Characteristics of Bacterial Pneumonia in Neurosurgical Emergency Center Patients: A Retrospective Study Spanning 13 Years at a Tertiary Center. Microorganisms, 11(8):1992. https://doi.org/10.3390/microorganisms11081992
Yusha’u, M., Aliyu, H., Kumurya, A., & Suleiman, K. (2010). Prevalence of Extended-spectrum β-lactamases (ESBLs) among Enterobacteriaceae in Murtala Mohammed Specialist Hospital, Kano, Nigeria. Bayero Journal of Pure and Applied Sciences, 3(1) 169-172. https://doi.org/10.4314/bajopas.v3i1.58756
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Copyright (c) 2024 Saleh, Z. M., Umar, A. T., Baba, F. A., Adamu, M. T., Yarma, A. A., Isa, S., Manga, M. M., Lawal Garba
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